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页码: 7/1030 每页显示数量
Photoexcited Cryptochrome 1 Interacts With SPCHLESS to Regulate Stomatal Development in Arabidopsis
Authors: Li Chen 1 2, Xiaoli Cao , Yupeng Li 1 2, Minqing Liu 1, Yao Liu 1, Yan Guan 1 2, Jiaqi Ruan 1, Zhilei Mao 1 2, Wenxiu Wang 1 2, Hong-Quan Yang 1 2, Tongtong Guo
Journal: PLANT CELL AND ENVIRONMENT
PubMed ID: 39253954
Doi: 10.1111/pce.15123
In Situ Noninvasive Monitoring of Cell Secretions Based on MOFs/AAO Hybrid Membrane Induced Asymmetric Ion Transport
Authors: Zhaohan Wang , Yafeng Wu , Zhi Wang , Zhaoyan Tian , Yu Liu , Songqin Liu
Journal: Biosensors & bioelectronics
Doi: 10.1016/j.bios.2024.116735
Products & Services: Recombinant Proteins   
PICKLE-mediated nucleosome condensing drives H3K27me3 spreading for the inheritance of Polycomb memory during differentiation
Authors: Zhenwei Liang, ∙ Tao Zhu, ∙ Yaoguang Yu,∙ … ∙ Yuhai Cui ∙ Shangzhi Huang ∙ Chenlong Li
Journal: Molecular Cell
Doi: doi.org/10.1016/j.molcel.2024.08.018
Products & Services: Molecular Biology Reagents   
Epitope prime editing shields hematopoietic cells from CD123 immunotherapy for acute myeloid leukemia
Authors: Rui-Jin Ji , Guo-Hua Cao , Wei-Qiang Zhao , Mu-Yao Wang , Pan Gao, Yi-Zhou Zhang, Xue-Bin Wang, Hou-Yuan Qiu , Di-Di Chen, Xiao-Han Tong , Min Duan, Hao Yin, Ying Zhang
Journal: Cell Stem Cell
PubMed ID: 39353428
Doi: 10.1016/j.stem.2024.09.003
Products & Services: Synthetic sgRNA and crRNA Service   
Inhalation of Bioorthogonal Gene-Editable Spiky-Pollen Reprograms Tumor-Associated Macrophages for Lung Cancer Immunotherapy
Authors: Qianglan Lu , Ruiyue Chen , Fei Zeng, Zhiyong Liu, Yongchun Pan, Yanfeng Gao, Bangshun He and Yujun Song
Journal: Advanced Functional Materials
Doi: 10.1002/adfm.202408767
Products & Services: Synthetic sgRNA and crRNA Service   
Identification of an RNA-binding perturbing characteristic for thiopurine drugs and their derivatives to disrupt CELF1–RNA interaction
Authors: Yang Tan , Zhibo Zhao , Qingfang Han , Peipei Xu , Xiaopeng Shen , Yajun Jiang , Qiang Xu , Xingxin Wu
Journal: Nucleic Acids Research
PubMed ID: 39268573
Doi: 10.1093/nar/gkae788
Products & Services: Plasmid DNA Preparation   
Tuning the fluidity and protein corona of ultrasound-responsive liposomal nanovaccines to program T cell immunity in mice
Authors: Jia He , Chaoyu Wang , Xiao Fang , Junyao Li , Xueying Shen , Junxia Zhang , Cheng Peng , Hongjian Li , Sai Li , Jeffrey M Karp , Rui Kuai
Journal: Nature Communications
PubMed ID: 39284814
Doi: 10.1038/s41467-024-52104-z
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Structural characterization of the (deoxy)hypusination in Trichomonas vaginalis questions the bifunctionality of deoxyhypusine synthase
Authors: Elżbieta Wątor, Piotr Wilk, Paweł Kochanowski, Przemysław Grudnik
Journal: FEBS J
Doi: doi.org/10.1111/febs.17207
Products & Services: Gene Synthesis   
Pyroptotic-Spatiotemporally Selective Delivery of siRNA against Pyroptosis and Autoimmune Diseases
Authors: Zongyou Pan, Kaiwang Xu, Guanrui Huang, Haoran Hu, Huang Yang, Haotian Shen, Kaijie Qiu, Canlong Wang, Tengjing Xu, Xinning Yu, Jinhua Fang, Jiajie Wang, Yunting Lin, Jiacheng Dai, Yuting Zhong, Hongyun Song, Sunan Zhu, Siheng Wang, Zhuxing Zhou, Chuyue Sun, Zhaopeng Tang, Shiyao Liao, Guang Yang, Zhiyuan You, Xuesong Dai, Zhengwei Mao
Journal: Advanced Materials
PubMed ID: 39081086
Doi: 10.1002/adma.202407115
Products & Services: Recombinant Proteins   
SARS-CoV-2 ORF10 hijacking ubiquitination machinery reveals potential unique drug targeting sites
Authors: Kaixiang Zhu, Lili Song, Linyue Wang, Lei Hua, Ziyu Luo, Tongyun Wang, Bo Qin, Shuofeng Yuan, Xiaopan Gao, Wenyi Mi, Sheng Cui
Journal: Acta Pharmaceutica Sinica B
PubMed ID: 39309499
Doi: doi.org/10.1016/j.apsb.2024.05.018
Products & Services: Gene Synthesis   
Mitochondrial rewiring with small-molecule drug-free nanoassemblies unleashes anticancer immunity
Authors: Lulu Ren , Jianqin Wan , Xiaoyan Li , Jie Yao , Yan Ma , Fanchao Meng , Shusen Zheng , Weidong Han , Hangxiang Wang
Journal: Nature Communications
PubMed ID: 39227567
Doi: 10.1038/s41467-024-51945-y
Products & Services: Protein Reagents and Products   
Antimony Component Schottky Nanoheterojunctions as UltrasoundHeightened Pyroptosis Initiators for Sonocatalytic Immunotherapy
Authors: Jihu Nie, Nailin Yang , Shumin Sun , Li Wang , Zifan Pei, Jie Wu, Qiao Yu, Zhihui Han ,Youdong Chen , Liang Cheng
Journal: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
PubMed ID: 39305135
Doi: 10.1002/anie.202416426
EXTL3 and NPC1 are mammalian host factors for Autographa californica multiple nucleopolyhedrovirus infection
Authors: Yuege Huang , Hong Mei , Chunchen Deng , Wei Wang , Chao Yuan , Yan Nie , Jia-Da Li , Jia Liu
Journal: Nature Communications
PubMed ID: 39231976
Doi: 10.1038/s41467-024-52193-w
Rapid generation of long, chemically modified pegRNAs for prime editing
Authors: Xinlin Lei , Anhui Huang , Didi Chen , Xuebin Wang , Ruijin Ji , Jinlin Wang , Yizhou Zhang , Yuming Zhang , Shuhan Lu , Kun Zhang , Qiubing Chen , Ying Zhang , Hao Yin
Journal: Nature Biotechnology
PubMed ID: 39349835
Doi: doi.org/10.1038/s41587-024-02394-x
A bioorthogonal probiotic platform spatiotemporally releases nanobodies in deep tumor for cancer chemoimmunotherapy
Authors: Ruiyue Chen , Qianglan Lu , Fei Zeng , Shurong Qin , Yongchun Pan , Zhiyong Liu , Yuzhen Wang , Bangshun He , Wenjin Qi , Yujun Song
Journal: Materials Today
Doi: 10.1016/j.mattod.2024.09.002
Products & Services: Recombinant Proteins   
Open-Source Throttling of CD8+ T Cells in Brain with Low-Intensity Focused Ultrasound-Guided Sequential Delivery of CXCL10, IL-2, and aPD-L1 for Glioblastoma Immunotherapy
Authors: Lei Dong , Yini Zhu , Haoge Zhang , Lin Gao , Zhiqi Zhang , Xiaoxuan Xu , Leqian Ying , Lu Zhang , Yue Li , Zhengcheng Yun , Danqi Zhu , Chang Han , Tingting Xu , Hui Yang , Shenghong Ju , Xiaoyuan Chen , Haijun Zhang , Jinbing Xie
Journal: ADVANCED MATERIALS
PubMed ID: 39264011
Doi: 10.1002/adma.202407235
Products & Services: Recombinant Proteins   
Strontium-Modified porous attapulgite composite hydrogel scaffold with advanced angiogenic and osteogenic potential for bone defect repair
Authors: Chun Liu , Su Ni , Xiaoyu Wu , Linxiang Zhang , Ting Dai , Aiqin Wang , Hongbin Zhao
Journal: COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
Doi: doi.org/10.1016/j.compositesa.2024.108492
Identification of antimicrobial bioactive peptides from the camel milk protein lactoferrin: Molecular docking, molecular dynamic simulation, and in vitro study
Authors: Mohammed Alzain , Ehab M.M. Ali , Mazin Zamzami , Ishtiaq Qadri , Hani Choudhry , Kamel Chaieb , Bochra Kouidhi , Hisham N. Altayb
Journal: food and humanity
Doi: doi.org/10.1016/j.foohum.2024.100414
Products & Services: Peptide Synthesis   
Diffusiophoresis promotes phase separation and transport of biomolecular condensates
Authors: Viet Sang Doan , Ibraheem Alshareedah , Anurag Singh , Priya R Banerjee , Sangwoo Shin
Journal: Nature Communications
PubMed ID: 37461689
Doi: 10.1038/s41467-024-51840-6
Products & Services: Peptide Synthesis   
AntigenBoost: enhanced mRNA-based antigen expression through rational amino acid substitution
Authors: Yumiao Gao, Siran Zhu, Huichun Li, Xueting Hao, Wen Chen, Deng Pan, Zhikang Qian
Journal: BRIEFINGS IN BIOINFORMATICS
PubMed ID: 39400114
Doi: 10.1093/bib/bbae468
Products & Services: Gene Synthesis   
Arabidopsis cryptochromes interact with SOG1 to promote the repair of DNA double-strand breaks
Authors: Li Chen , Minqing Liu , Yupeng Li , Yan Guan, Jiaqi Ruan , Zhilei Mao , Wenxiu Wang , Hong-Quan Yang , Tongtong Guo
Journal: BBRC
Doi: 10.1016/j.bbrc.2024.150233
Products & Services: Catalog Antibody   
The 8-oxoguanine DNA glycosylase-synaptotagmin 7 pathway increases extracellular vesicle release and promotes tumour metastasis during oxidative stress
Authors: Ying Ma , Jiarong Guo , Haipeng Rao , Jingyu Xin , Xinyi Song , Rui Liu , Shan Shao , Jiajia Hou , Liyu Kong , Zhigang Hu , Lingfeng He , Feiyan Pan , Zhigang Guo
Journal: Journal of extracellular vesicles
PubMed ID: 39235072
Doi: 10.1002/jev2.12505
Products & Services: Gene Synthesis   
Clathrin-associated carriers enable recycling through a kiss-and-run mechanism
Authors: Jiachao Xu, Yu Liang, Nan Li, Song Dang, Amin Jiang, Yiqun Liu, Yuting Guo, Xiaoyu Yang, Yi Yuan, Xinyi Zhang, Yaran Yang, Yongtao Du, Anbing Shi, Xiaoyun Liu, Dong Li, Kangmin He
Journal: Nature Cell Biology
PubMed ID: 39300312
Doi: 10.1038/s41556-024-01499-4
Products & Services: Molecular Biology Reagents   
Chemoproteomic profiling unveils binding and functional diversity of endogenous proteins that interact with endogenous triplex DNA
Authors: Hongzhan Xu , Jing Ye # , Kui-Xing Zhang # , Qingxi Hu , Tongxiao Cui , Chong Tong , Mengqi Wang , Huichao Geng , Kun-Ming Shui , Yan Sun , Jian Wang , Xiaomeng Hou , Kai Zhang , Ran Xie , Yafei Yin , Nan Chen , Jia-Yu Chen
Journal: nature Chemistry
PubMed ID: 39223307
Doi: 10.1038/s41557-024-01609-7
Products & Services: Catalog Peptides   
OsFAD1–OsMYBR22 modulates clustered spikelet through regulating BRD3 in rice
Authors: Mingxing Cheng , Huanran Yuan , Ruihua Wang , Fengfeng Fan , Fengfeng Si , Xiong Luo , Wei Liu , Shaoqing Li
Journal: Journal of integrative plant biology
PubMed ID: 39279451
Doi: doi.org/10.1111/jipb.13775
Products & Services: Peptide Synthesis   
OsGRF6-OsYUCCA1/OsWRKY82 Signaling Cascade Upgrade Grain Yield and Bacterial Blight Resistance in Rice
Authors: Huanran Yuan, Mingxing Cheng, Fengfeng Fan , Xingfei Zheng , Ruihua Wang , Fengfeng Si , Xiong Luo, Nengwu Li 1, Shaoqing Li
Journal: Advanced Science
PubMed ID: 38923395
Doi: 10.1002/advs.202407733
Products & Services: Gene Synthesis   
Translation of Overlapping Open Reading Frames Promoted by Type 2 IRESs in Avian Calicivirus Genomes
Authors: Yani Arhab , Tatyana V Pestova , Christopher U T Hellen
Journal: Viruses
PubMed ID: 39339889
Doi: 10.3390/v16091413
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Discovery of alkaline laccases from basidiomycete fungi through machine learning-based approach
Authors: Xing Wan, Sazzad Shahrear, Shea Wen Chew, Francisco Vilaplana, Miia R Mäkelä
Journal: Biotechnol Biofuels Bioprod
PubMed ID: 39261970
Doi: 10.1186/s13068-024-02566-6
Products & Services: Gene Synthesis   
A two-pronged nanostrategy of iron metabolism disruption to synergize tumor therapy by triggering the paraptosis-apoptosis hybrid pathway
Authors: Huali Lei , Guanghui Hou , Lin Liu , Zifan Pei , Youdong Chen , Yujie Lu , Nalin Yang , Shumin Sun , Liang Cheng
Journal: ACS Nano
PubMed ID: 39121010
Doi: 10.1021/acsnano.4c06199
Products & Services: Peptide Synthesis   
25-Hydroxycholesterol inhibits Hantavirus infection by reprogramming cholesterol metabolism
Authors: Yamei Dang , Yuan Wang , Jing Wei , Hui Zhang , Qiqi Yang , Bin Wang , Jia Li , Chuantao Ye , Yang Chen , Peijun Han , Xiaolei Jin , Jia Wang , Xiaohui Bao , He Liu , Hongwei Ma , Liang Zhang , Linfeng Cheng , Yangchao Dong , Yinlan Bai , Yinghui Li , Yingfeng Lei , Zhikai Xu , Fanglin Zhang , Wei Ye
Journal: FREE RADICAL BIOLOGY AND MEDICINE
PubMed ID: 39209137
Doi: 10.1016/j.freeradbiomed.2024.08.029
Products & Services: Codon Optimization   
Activation of ATF3 via the integrated stress response pathway regulates innate immune response to restrict Zika virus
Authors: Pheonah Badu , Gabriele Baniulyte , Morgan A Sammons , Cara T Pager
Journal: Virology Journal
PubMed ID: 39212382
Doi: 10.1128/jvi.01055-24
Products & Services: CRISPR RNAs/Cas9 Proteins(INACTIVE)   
Reduced Cross-Protective Potential of Omicron Compared to Ancestral SARS-CoV-2 Spike Vaccines Against Potentially Zoonotic Coronaviruses
Authors: Tyler RennerMatthew StuibleBrian CassSylvie PerretJulie GuimondSimon Lord-DufourMichael J. McCluskieYves DurocherBassel Akache
Journal: npj Viruses
Doi: doi.org/10.21203/rs.3.rs-4791122/v1
Products & Services: Gene Synthesis   PCR Cloning and Subcloning   
Transcription elongation of the plant RNA polymerase IV is prone to backtracking
Authors: Chengli Fang, Kun Huang, Xiaoxian Wu, Hongwei Zhang, Zhanxi Gu, Jiawei Wang, Yu Zhang
Journal: Science Advances
PubMed ID: 39178250
Doi: 10.1126/sciadv.adq3087
A maternal brain hormone that builds bone
Authors: Muriel E Babey, William C Krause, Kun Chen, Candice B Herber, Zsofia Torok, Joni Nikkanen, Ruben Rodriguez, Xiao Zhang, Fernanda Castro-Navarro, Yuting Wang, Erika E Wheeler, Saul Villeda, J Kent Leach, Nancy E Lane, Erica L Scheller, Charles K F Chan, Thomas H Ambrosi, Holly A Ingraham
Journal: Nature
PubMed ID: 38987585
Products & Services: Peptide Synthesis   
Preparation of polyclonal antibody against thrombospondin 2 recombinant protein and its functional verification in pulmonary hypertension syndrome in broilers
Authors: Wen Peng, Chenxi Jiang, Gaofeng Cai, Liling Liu, Xiaoquan Guo, Xiaona Gao, Guyue Li, Zhanhong Zheng, Pei Liu, Ping Liu
Journal: International Journal of Biological Macromolecules
PubMed ID: 38906344
Doi: doi.org/10.1016/j.ijbiomac.2024.133284
Proteasome gene expression is controlled by coordinated functions of multiple transcription factors
Authors: Jennifer E Gilda, Asrafun Nahar, Dharanibalan Kasiviswanathan, Nadav Tropp, Tamar Gilinski, Tamar Lahav, Dina Alexandrovich, Yael Mandel-Gutfreund, Soyeon Park, Shenhav Shemer
Journal: J Cell Biol
PubMed ID: 38767572
Doi: 10.1083/jcb.202402046
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Secreted PTEN binds PLXDC2 on macrophages to drive antitumor immunity and tumor suppression
Authors: Cheng Zhang , Hong-Ming Ma , Shuai Wu , Jia-Ming Shen , Na Zhang , Yi-Lu Xu , Cheng-Xiao Li , Ping He , Meng-Kai Ge , Xi-Li Chu , Yu-Xue Zhang , Jun-Ke Zheng , Guo-Qiang Chen , Shao-Ming Shen
Journal: Developmental Cell
PubMed ID: 39197453
Doi: 10.1016/j.devcel.2024.08.003
Activation mechanisms of dimeric mechanosensitive OSCA/TMEM63 channels
Authors: Yuanyue Shan , Mengmeng Zhang , Meiyu Chen , Xinyi Guo , Ying Li , Mingfeng Zhang , Duanqing Pei
Journal: Nature Communications
PubMed ID: 39209849
Doi: 10.1038/s41467-024-51800-0.
Systematic identification of minor histocompatibility antigens predicts outcomes of allogeneic hematopoietic cell transplantation
Authors: Nicoletta Cieri 1 2 3, Nidhi Hookeri 1 2 4, Kari Stromhaug 1 2, Liang Li 2, Julia Keating 4, Paula Díaz-Fernández 5, Valle Gómez-García de Soria 6, Jonathan Stevens 7, Raphael Kfuri-Rubens 1 2, Yiren Shao 1 2 4, Kameron A Kooshesh 3, Kaila Powell 1, Helen Ji 1, Gabrielle M Hernandez 2, Jennifer Abelin 2, Susan Klaeger 2 8, Cleo Forman 1 4, Karl R Clauser 2, Siranush Sarkizova 2, David A Braun 1 2 3 9 10, Livius Penter 1 2 3 11, Haesook T Kim 4, William J Lane 3 7, Giacomo Oliveira 1 2 3, Leslie S Kean 3 12, Shuqiang Li 1 13, Kenneth J Livak 1 13, Steven A Carr 2, Derin B Keskin 1 2 3 13 14 15, Cecilia Muñoz-Calleja 5 16, Vincent T Ho 1 3 9, Jerome Ritz 1 3 9, Robert J Soiffer 1 3 9, Donna Neuberg 4, Chip Stewart 2, Gad Getz 2 3 17 18, Catherine J Wu 19 20 21 22
Journal: Nat Biotechnol
PubMed ID: 39169264
Doi: 10.1038/s41587-024-02348-3
Products & Services: Peptides and Peptide Library   
A metal anion strategy to induce pyroptosis combined with STING activation to synergistically amplify anti-tumor immunity
Authors: Zifan Pei , Nan Jiang , Fei Gong , Weihao Yang , Jiachen Xu , Bin Yu , Nailin Yang , Jie Wu , Huali Lei , Shumin Sun , Longxiao Li , Zhicheng Liu , Caifang Ni , Liang Cheng
Journal: Materials Today
Doi: 10.1016/j.mattod.2024.07.013
hypocretin-1/hypocretin receptor 1 regulates neuroplasticity and cognitive function through hippocampal lactate homeostasis in depressed model
Authors: Bing Chen , Kangyu Jin , Jingyi Dong , Shangping Cheng , Lingzhuo Kong , Shaohua Hu , Zuobing Chen, Jing Lu
Journal: Advanced Science
PubMed ID: 39119889
Doi: 10.1002/advs.202405354
A low-molecular-weight α-glucan from edible fungus Agaricus blazei Murrill activates macrophage TFEB-mediated antibacterial defense to combat implant-associated infection
Authors: Qishan Wang , Yuehong Wang , Yihao Liu , Kai Yuan , Yixuan Lin , Xian Qian , Hongyan Pei , Liangliang Weng , Kaijian Fan , Yihe Hu , Yiqi Yang
Journal: Carbohydrate Polymers
Doi: 10.1016/j.carbpol.2024.122659
Spatiotemporal coordination of antigen presentation and co-stimulatory signal for enhanced anti-tumor vaccination
Authors: Sha Peng , Yumeng Yan , Kenji Ogino , Guanghui Ma , Yufei Xia
Journal: Journal of controlled release
PubMed ID: 39153722
Doi: 10.1016/j.jconrel.2024.08.025.
Products & Services: Peptoid Synthesis   
Rupatadine-inhibited OTUD3 promotes DLBCL progression and immune evasion through deubiquitinating MYL12A and PD-L1
Authors: Ying Sui , Ziyang Shen, Xiaoyou Li 1, Ya Lu , SiTong Feng , Rong Ma, Jianzhong Wu , Changwen Jing, Zhuo Wang, Jifeng Feng , Haixia Cao
Journal: Cell death & disease
PubMed ID: 39097608
Doi: 10.038/s41419-024-06941-x
siRNA nanoparticle targeting Usp20 lowers lipid levels and ameliorates metabolic syndrome in mice
Authors: Yi Ding 1, Qiu-Bing Chen , Hui Xu , Dilare Adi , Yi-Wen Ding , Wen-Jun Luo , Wen-Zhuo Zhu , Jia-Chen Xu , Xiaolu Zhao , Xiong-Jie Shi , Jie Luo , Hao Yin , Xiao-Yi Lu
Journal: Journal of lipid research
PubMed ID: 39173829
Doi: 10.1016/j.jlr.2024.100626
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Channelrhodopsins with distinct chromophores and binding patterns
Authors: Yuanyue Shan , Liping Zhao , Meiyu Chen , Xiao Li , Mingfeng Zhang , Duanqing Pei
Journal: Nature Communications
PubMed ID: 39181878
Doi: 10.1038/s41467-024-51811-x
Human mitochondrial carriers of the SLC25 family function as monomers exchanging substrates with a ping-pong kinetic mechanism
Authors: Camila Cimadamore-Werthein , Martin S King , Denis Lacabanne , Eva Pyrihová , Stephany Jaiquel Baron , Edmund Rs Kunji
Journal: EMBO J
PubMed ID: 38937634
Doi: 10.1038/s44318-024-00150-0.
Products & Services: Gene Synthesis   
Anattachment glycoprotein nanoparticle elicits broadly neutralizing antibodies and protects against lethal Nipah virus infection
Authors: Dan Zhou , Rao Cheng , Yanfeng Yao , Gan Zhang , Xin Li , Bingjie Wang , Yong Wang , Feiyang Yu , Shangyu Yang , Hang Liu , Ge Gao , Yun Peng , Miaoyu Chen , Zengqin Deng , Haiyan Zhao
Journal: npj Vaccines
PubMed ID: 39217188
Doi: 10.1038/s41541-024-00954-5
Cellular N-Myristoyl Transferases Are Required for Mammarenavirus Multiplication
Authors: Haydar Witwit , Carlos Alberto Betancourt , Beatrice Cubitt , Roaa Khafaji , Heinrich Kowalski , Nathaniel Jackson , Chengjin Ye , Luis Martinez-Sobrido , Juan C de la Torre
Journal: Viruses
PubMed ID: 39339839
Doi: 10.3390/v16091362
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Dynamics and implications of anti-drug antibodies against adalimumab using ultra-sensitive and highly drug-tolerant assays
Authors: Xiaoliang Ding, Ling Xue, Mingjun Wang, Shengxiong Zhu, Kouzhu Zhu, Sheng Jiang, Jian Wu, Liyan Miao
Journal: Frontiers in Immunology
PubMed ID: 39238635
Doi: 10.3389/fimmu.2024.1429544
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