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Defective brown adipose tissue thermogenesis and impaired glucose metabolism in mice lacking Letmd1
Authors: Kyung-Mi Choi, Jung Hak Kim, Xiangmudong Kong, Meltem Isik, Jin Zhang, Hee-Woong Lim, John C Yoon
Journal: Cell Rep
PubMed ID: 34910916
Doi: 10.1016/j.celrep.2021.110104
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VLDLR and ApoER2 are receptors for multiple alphaviruses
Authors: Lars E Clark, Sarah A Clark, ChieYu Lin, Jianying Liu, Adrian Coscia, Katherine G Nabel, Pan Yang, Dylan V Neel, Hyo Lee, Vesna Brusic, Iryna Stryapunina, Kenneth S Plante, Asim A Ahmed, Flaminia Catteruccia, Tracy L Young-Pearse, Isaac M Chiu, Paula Montero Llopis, Scott C Weaver, Jonathan Abraham
Journal: Nature
PubMed ID: 34929721
Doi: 10.1038/s41586-021-04326-0
Products & Services: Mammalian Expression   
Bombyx mori Nucleopolyhedrovirus (BmNPV) Induces G2/M Arrest to Promote Viral Multiplication by Depleting
Authors: Qin Xiao, Zhan-Qi Dong, Yan Zhu, Qian Zhang, Xiu Yang, Miao Xiao, Peng Chen, Cheng Lu, Min-Hui Pan
Journal: Insects
PubMed ID: 34940186
Doi: 10.3390/insects12121098
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Foxp3+ CD4+ regulatory T cells control dendritic cells in inducing antigen-specific immunity to emerging SARS-CoV-2 antigens
Authors: Ryuta Uraki, Masaki Imai, Mutsumi Ito, Hiroaki Shime, Mizuyu Odanaka, Moe Okuda, Yoshihiro Kawaoka, Sayuri Yamazaki
Journal: PLoS Pathog
PubMed ID: 34882757
Doi: 10.1371/journal.ppat.1010085
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Phospholipid nanoparticles: Therapeutic potentials against atherosclerosis via reducing cholesterol crystals and inhibiting inflammation
Authors: Yonghong Luo, Yanhong Guo, Huilun Wang, Minzhi Yu, Kristen Hong, Dan Li, Ruiting Li, Bo Wen, Die Hu, Lin Chang, Jifeng Zhang, Bo Yang, Duxin Sun, Anna S Schwendeman, Y Eugene Chen
Journal: EBioMedicine
PubMed ID: 34879325
Doi: 10.1016/j.ebiom.2021.103725
Products & Services: Peptide Synthesis   
Protective effects of combining monoclonal antibodies and vaccines against the Plasmodium falciparum circumsporozoite protein
Authors: Lawrence T Wang, Lais S Pereira, Patience K Kiyuka, Arne Schön, Neville K Kisalu, Rachel Vistein, Marlon Dillon, Brian G Bonilla, Alvaro Molina-Cruz, Carolina Barillas-Mury, Joshua Tan, Azza H Idris, Joseph R Francica, Robert A Seder
Journal: PLoS Pathog
PubMed ID: 34871332
Doi: 10.1371/journal.ppat.1010133
Products & Services: Peptide Synthesis   
Oxidation of Various Kraft Lignins with a Bacterial Laccase Enzyme
Authors: Sebastian A Mayr, Raditya Subagia, Renate Weiss, Nikolaus Schwaiger, Hedda K Weber, Johannes Leitner, Doris Ribitsch, Gibson S Nyanhongo, Georg M Guebitz
Journal: Int J Mol Sci
PubMed ID: 34884966
Doi: 10.3390/ijms222313161
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A structurally conserved site in AUP1 binds the E2 enzyme UBE2G2 and is essential for ER-associated degradation
Authors: Christopher E Smith, Yien Che Tsai, Yu-He Liang, Domarin Khago, Jennifer Mariano, Jess Li, Sergey G Tarasov, Emma Gergel, Borong Tsai, Matthew Villaneuva, Michelle E Clapp, Valentin Magidson, Raj Chari, R Andrew Byrd, Xinhua Ji, Allan M Weissman
Journal: PLoS Biol
PubMed ID: 34879065
Doi: 10.1371/journal.pbio.3001474
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Metabolic drug survey highlights cancer cell dependencies and vulnerabilities
Authors: Tea Pemovska, Johannes W Bigenzahn, Ismet Srndic, Alexander Lercher, Andreas Bergthaler, Adrián César-Razquin, Felix Kartnig, Christoph Kornauth, Peter Valent, Philipp B Staber, Giulio Superti-Furga
Journal: Nat Commun
PubMed ID: 34907165
Doi: 10.1038/s41467-021-27329-x
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Calibration of two validated SARS-CoV-2 pseudovirus neutralization assays for COVID-19 vaccine evaluation
Authors: Yunda Huang, Oleg Borisov, Jia Jin Kee, Lindsay N Carpp, Terri Wrin, Suqin Cai, Marcella Sarzotti-Kelsoe, Charlene McDanal, Amanda Eaton, Rolando Pajon, John Hural, Christine M Posavad, Katherine Gill, Shelly Karuna, Lawrence Corey, M Juliana McElrath, Peter B Gilbert, Christos J Petropoulos, David C Montefiori
Journal: Sci Rep
PubMed ID: 34907214
Doi: 10.1038/s41598-021-03154-6
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Identification and Characterization Analysis of Transient Receptor Potential Mucolipin Protein of Fallén
Authors: Haitao Wang, Yan Dong, Baijie Wan, Yinghua Ji, Qiufang Xu
Journal: Insects
PubMed ID: 34940195
Doi: 10.3390/insects12121107
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The parasitophorous vacuole nutrient channel is critical for drug access in malaria parasites and modulates the artemisinin resistance fitness cost
Authors: Paolo Mesén-Ramírez, Bärbel Bergmann, Mourad Elhabiri, Lei Zhu, Heidrun von Thien, Carolina Castro-Peña, Tim-Wolf Gilberger, Elisabeth Davioud-Charvet, Zbynek Bozdech, Anna Bachmann, Tobias Spielmann
Journal: Cell Host Microbe
PubMed ID: 34863371
Doi: 10.1016/j.chom.2021.11.002
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The NSP14/NSP10 RNA repair complex as a Pan-coronavirus therapeutic target
Authors: Gergely Rona, Andras Zeke, Bearach Miwatani-Minter, Maren de Vries, Ramanjit Kaur, Austin Schinlever, Sheena Faye Garcia, Hailey V Goldberg, Hui Wang, Thomas R Hinds, Fabrice Bailly, Ning Zheng, Philippe Cotelle, Didier Desmaële, Nathaniel R Landau, Meike Dittmann, Michele Pagano
Journal: Cell Death Differ
PubMed ID: 34862481
Doi: 10.1038/s41418-021-00900-1
Products & Services: Codon Optimization   
Development and characterization of efficient xylose utilization strains of Zymomonas mobilis
Authors: Jiyun Lou, Jingwen Wang, Yongfu Yang, Qing Yang, Runxia Li, Mimi Hu, Qiaoning He, Jun Du, Xia Wang, Mian Li, Shihui Yang
Journal: Biotechnol Biofuels
PubMed ID: 34863266
Doi: 10.1186/s13068-021-02082-x
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GR-mediated transcriptional regulation of mA metabolic genes contributes to diet-induced fatty liver in hens
Authors: Yue Feng, Yanlin Li, Wenduo Jiang, Yun Hu, Yimin Jia, Ruqian Zhao
Journal: J Anim Sci Biotechnol
PubMed ID: 34872591
Doi: 10.1186/s40104-021-00642-7
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Amantadine has potential for the treatment of COVID-19 because it inhibits known and novel ion channels encoded by SARS-CoV-2
Authors: Trine Lisberg Toft-Bertelsen, Mads Gravers Jeppesen, Eva Tzortzini, Kai Xue, Karin Giller, Stefan Becker, Amer Mujezinovic, Bo Hjorth Bentzen, Loren B Andreas, Antonios Kolocouris, Thomas Nitschke Kledal, Mette Marie Rosenkilde
Journal: Commun Biol
PubMed ID: 34853399
Doi: 10.1038/s42003-021-02866-9
Products & Services: Gene Synthesis   
Immune responses in multiple hosts to Nucleocapsid Protein (NP) of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)
Authors: Elif Karaaslan, Nesibe Selma Çetin, Merve Kalkan-Yazıcı, Sevde Hasanoğlu, Faruk Karakeçili, Aykut Özdarendeli, Ahmet Kalkan, Ali Osman Kılıç, Mehmet Ziya Doymaz
Journal: PLoS Negl Trop Dis
PubMed ID: 34851958
Doi: 10.1371/journal.pntd.0009973
Products & Services: Bacterial Expression   
Division of labor between SOS and PafBC in mycobacterial DNA repair and mutagenesis
Authors: Oyindamola O Adefisayo, Pierre Dupuy, Astha Nautiyal, James M Bean, Michael S Glickman
Journal: Nucleic Acids Res
PubMed ID: 34871411
Doi: 10.1093/nar/gkab1169
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FOXN1 forms higher-order nuclear condensates displaced by mutations causing immunodeficiency
Authors: Ioanna A Rota, Adam E Handel, Stefano Maio, Fabian Klein, Fatima Dhalla, Mary E Deadman, Stanley Cheuk, Joseph A Newman, Yale S Michaels, Saulius Zuklys, Nicolas Prevot, Philip Hublitz, Philip D Charles, Athina Soragia Gkazi, Eleni Adamopoulou, Waseem Qasim, Edward Graham Davies, Imelda Hanson, Alistair T Pagnamenta, Carme Camps, Helene M Dreau, Andrea White, Kieran James, Roman Fischer, Opher Gileadi, Jenny C Taylor, Tudor Fulga, B Christoffer Lagerholm, Graham Anderson, Erdinc Sezgin, Georg A Holländer
Journal: Sci Adv
PubMed ID: 34860543
Doi: 10.1126/sciadv.abj9247
Mining Marine Metagenomes Revealed a Quorum-Quenching Lactonase with Improved Biochemical Properties That Inhibits the Food Spoilage Bacterium Pseudomonas fluorescens
Authors: Rami Haramati, Shlomit Dor, David Gurevich, Doron Levy, Dekel Freund, Giora Rytwo, Itai Sharon, Livnat Afriat-Jurnou
Journal: Appl Environ Microbiol
PubMed ID: 34910563
Doi: 10.1128/AEM.01680-21
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Isolation of Healthy F4/80+ Macrophages from Embryonic day E135 Mouse Fetal Liver Using Magnetic Nanoparticles for Single Cell Sequencing
Authors: Kaustav Mukherjee, James J Bieker
Journal: Bio Protoc
PubMed ID: 35005088
Doi: 10.21769/BioProtoc.4243
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Long non‑coding RNA LINC01748 exerts carcinogenic effects in non‑small cell lung cancer cell lines by regulating the microRNA‑520a‑5p/HMGA1 axis
Authors: Yinling Tan, Fengxia Xu, Lingling Xu, Jianying Cui
Journal: Int J Mol Med
PubMed ID: 34970695
Doi: 10.3892/ijmm.2021.5077
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Preparation and characterization of a fully human monoclonal antibody specific for human tumor necrosis factor alpha
Authors: Xinmei Liao, Hui Liang, Jian Pan, Qian Zhang, Jiaqi Niu, Cuili Xue, Jian Ni, Daxiang Cui
Journal: Bioengineered
PubMed ID: 34886761
Doi: 10.1080/21655979.2021.1967710
Fourier-Transform Approach for Reconstructing Macromolecular Mass Defect Profiles
Authors: Andrew K Swansiger, Michael T Marty, James S Prell
Journal: J Am Soc Mass Spectrom
PubMed ID: 34913687
Doi: 10.1021/jasms.1c00317
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HMGA2 promotes the migration and invasion of gallbladder cancer cells and HMGA2 knockdown inhibits angiogenesis via targeting VEGFA
Authors: Jun Yan, Peng Dai, Xueliang Qin, Yanping He, Yu Zhang
Journal: Mol Med Rep
PubMed ID: 34913073
Doi: 10.3892/mmr.2021.12570
Products & Services: Plasmid DNA Preparation   
Development of a recombinant nucleocapsid protein-based ELISA for the detection of IgM and IgG antibodies to SARS-CoV-2
Authors: Maryam Ranjbar, Marzieh Asadi, Marjan Nourigorji, Bahador Sarkari, Zohreh Mostafavi-Pour, Kamiar Zomorodian, Zahra Shabaninejad, Mortaza Taheri-Anganeh, Amir Maleksabet, Mohsen Moghadami, Amir Savardashtaki
Journal: Biotechnol Appl Biochem
PubMed ID: 34965611
Doi: 10.1002/bab.2308
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Loss of Neutralizing Antibody Response to mRNA Vaccination against SARS-CoV-2 Variants: Differing Kinetics and Strong Boosting by Breakthrough Infection
Authors: John P Evans, Cong Zeng, Claire Carlin, Gerard Lozanski, Linda J Saif, Eugene M Oltz, Richard J Gumina, Shan-Lu Liu
Journal: biorxiv
PubMed ID: 34909777
Doi: 10.1101/2021.12.06.471455
Products & Services: Stable Cell Lines   
Tanshinone IIA sodium sulfonate attenuates inflammation by upregulating circ-Sirt1 and inhibiting the entry of NF-κB into the nucleus
Authors: Jiao Lan, Ke Li, Austin Gresham, Jifei Miao
Journal: Eur J Pharmacol
PubMed ID: 34896110
Doi: 10.1016/j.ejphar.2021.174693
Products & Services: Endotoxin Detection & Removal System   
Fine-tuning of pathogenesis-related protein 1 (PR1) activity by the melatonin biosynthetic enzyme ASMT2 in defense response to cassava bacterial blight
Authors: Jingru Guo, Yujing Bai, Yunxie Wei, Yabin Dong, Hongqiu Zeng, Russel J Reiter, Haitao Shi
Journal: J Pineal Res
PubMed ID: 34936113
Doi: 10.1111/jpi.12784
Comparative analysis of mussel foot protein 3B co-expressed with tyrosinases provides a potential adhesive biomaterial
Authors: Lin Yao, Xinyi Wang, Rui Xue, Hong Xu, Rui Wang, Lujia Zhang, Sha Li
Journal: Int J Biol Macromol
PubMed ID: 34896153
Doi: 10.1016/j.ijbiomac.2021.11.208
Products & Services: Bacterial Expression   
Deferasirox combination with eltrombopag shows anti-myelodysplastic syndrome effects by enhancing iron deprivation-related apoptosis
Authors: Lei Huang, Mengyue Tian, Zhaoyun Liu, Chunyan Liu, Rong Fu
Journal: J Investig Med
PubMed ID: 34921125
Doi: 10.1136/jim-2021-002147
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Cryopreservation of network bioactivity and multi-lineage stromal cell differentiation potential within three-dimensional synthetic hydrogels
Authors:
Journal: Cryobiology
PubMed ID: 34922883
Doi: 10.1016/j.cryobiol.2021.12.003
Products & Services: Molecular Biology Reagents   
Developing a secretory AcGFP1-based IRES expression system for efficient production of mammalian recombinant proteins
Authors: Zhongcheng Zou, Matthew Spencer, Peter D Sun
Journal: Protein Expr Purif
PubMed ID: 34920134
Doi: 10.1016/j.pep.2021.106029
Products & Services: Codon Optimization   
Structural Insight into the Mechanism of PALB2 Interaction with MRG15
Authors: Jennifer Redington, Jaigeeth Deveryshetty, Lakshmi Kanikkannan, Ian Miller, Sergey Korolev
Journal: Genes (Basel)
PubMed ID: 34946951
Doi: 10.3390/genes12122002
Products & Services: Mutant Libraries   
Reversible myocardial injury aggravated by complex arrhythmias in three Toxoplasma gondii-positive dogs
Authors: Giovanni Romito, Elena Venturelli, Vanna Tintorri, Mario Cipone
Journal: J Vet Med Sci
PubMed ID: 34955461
Doi: 10.1292/jvms.21-0571
Products & Services: PCR and Cloning   
Engineered Sphingomonas sp KT-1 PahZ1 monomers efficiently degrade poly(aspartic acid)
Authors: Timothy Lamantia, Amanda Jansch, Justin D Marsee, Mitch H Weiland, Justin M Miller
Journal: Biophys Chem
PubMed ID: 34953381
Doi: 10.1016/j.bpc.2021.106745
Products & Services: Mutagenesis Services   
CASP5 and CR1 as potential biomarkers for Kawasaki disease: an Integrated Bioinformatics-Experimental Study
Authors: Yazdan Rahmati, Hasan Mollanoori, Sajad Najafi, Sajjad Esmaeili, Mohammad Reza Alivand
Journal: BMC Pediatr
PubMed ID: 34895171
Doi: 10.1186/s12887-021-03003-5
Products & Services: Molecular Biology Tools   
Hydroxysafflor yellow A inhibits the proliferation, migration, and invasion of colorectal cancer cells through the PPARγ/PTEN/Akt signaling pathway
Authors: Dan Su, Chunye Lv
Journal: Bioengineered
PubMed ID: 34889713
Doi: 10.1080/21655979.2021.2009965
Products & Services: Gene Synthesis   
Increased sensitivity of gp210 autoantibody detection using a newly designed gp210 antigen
Authors: Jing Xu, Haoyi Zhang, Chan Wang, Peng Jiang, Chongxu Han, Yaping Dai, Fang Qiu, Yuhua Gong, Yuzhang Jiang, Ping Xu, Mingming Zhang, Luyao Zhang, Xingjuan Shi, Sufang Chen, Ye Tian, Michael F Seldin, M Eric Gershwin, Xiangdong Liu, Li Li
Journal: J Immunol Methods
PubMed ID: 34971632
Doi: 10.1016/j.jim.2021.113211
Products & Services: Mammalian Expression   
Vulnerable exons, like ACADM exon 5, are highly dependent on maintaining a correct balance between splicing enhancers and silencers
Authors: Lise L Holm, Thomas K Doktor, Michael B Hansen, Ulrika S S Petersen, Brage S Andresen
Journal: Hum Mutat
PubMed ID: 34923709
Doi: 10.1002/humu.24321
Products & Services: Mutagenesis Services   
Increased sensitivity of gp210 autoantibody detection using a newly designed gp210 antigen
Authors: Jing Xu, Haoyi Zhang, Chan Wang, Peng Jiang, Chongxu Han, Yaping Dai, Fang Qiu, Yuhua Gong, Yuzhang Jiang, Ping Xu, Mingming Zhang, Luyao Zhang, Xingjuan Shi, Sufang Chen, Ye Tian, Michael F Seldin, M Eric Gershwin, Xiangdong Liu, Li Li
Journal: J Immunol Methods
PubMed ID: 34971632
Doi: 10.1016/j.jim.2021.113211
Products & Services: Mammalian Expression   
CTLA4-Ig treatment induces M1-M2 shift in cultured monocyte-derived macrophages from healthy subjects and rheumatoid arthritis patients
Authors: Maurizio Cutolo, Stefano Soldano, Emanuele Gotelli, Paola Montagna, Rosanna Campitiello, Sabrina Paolino, Carmen Pizzorni, Alberto Sulli, Vanessa Smith, Samuele Tardito
Journal: Arthritis Res Ther
PubMed ID: 34952630
Doi: 10.1186/s13075-021-02691-9
The microbial gbu gene cluster links cardiovascular disease risk associated with red meat consumption to microbiota L-carnitine catabolism
Authors: Jennifer A Buffa, Kymberleigh A Romano, Matthew F Copeland, David B Cody, Weifei Zhu, Rachel Galvez, Xiaoming Fu, Kathryn Ward, Marc Ferrell, Hong J Dai, Sarah Skye, Ping Hu, Lin Li, Mirjana Parlov, Amy McMillan, Xingtao Wei, Ina Nemet, Robert A Koeth, Xinmin S Li, Zeneng Wang, Naseer Sangwan, Adeline M Hajjar, Mohammed Dwidar, Taylor L Weeks, Nathalie Bergeron, Ronald M Krauss, W H Wilson Tang, Federico E Rey, Joseph A DiDonato, Valentin Gogonea, G Frank Gerberick, Jose Carlos Garcia-Garcia, Stanley L Hazen
Journal: Nat Microbiol
PubMed ID: 34949826
Doi: 10.1038/s41564-021-01010-x
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The ecdysteroid receptor regulates salivary gland degeneration through apoptosis in Rhipicephalus haemaphysaloides
Authors: Xiaojuan Lu, Zhipeng Zhang, Dongqi Yuan, Yongzhi Zhou, Jie Cao, Houshuang Zhang, Itabajara da Silva Vaz, Jinlin Zhou
Journal: Parasit Vectors
PubMed ID: 34930413
Doi: 10.1186/s13071-021-05052-2
The key regulator LcERF056 enhances salt tolerance by modulating reactive oxygen species-related genes in Lotus corniculatus
Authors: Dan Wang, Zhanmin Sun, Xinxu Hu, Junbo Xiong, Lizhen Hu, Yuandong Xu, Yixiong Tang, Yanmin Wu
Journal: BMC Plant Biol
PubMed ID: 34965872
Doi: 10.1186/s12870-021-03336-4
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Exposure to in Asian Elephants () in Thailand
Authors: Ruenruetai Udonsom, Yoshifumi Nishikawa, Ragab M Fereig, Thitirat Topisit, Natchakorn Kulkaweewut, Supitcha Chanamrung, Charoonluk Jirapattharasate
Journal: Pathogens
PubMed ID: 35055950
Doi: 10.3390/pathogens11010002
TIN2 is an architectural protein that facilitates TRF2-mediated trans- and cis-interactions on telomeric DNA
Authors: Parminder Kaur, Ryan Barnes, Hai Pan, Ariana C Detwiler, Ming Liu, Chelsea Mahn, Jonathan Hall, Zach Messenger, Changjiang You, Jacob Piehler, Robert C Smart, Robert Riehn, Patricia L Opresko, Hong Wang
Journal: Nucleic Acids Res
PubMed ID: 34883513
Doi: 10.1093/nar/gkab1142
Angiopoietin-2 silence alleviates lipopolysaccharide-induced inflammation, barrier dysfunction and endoplasmic reticulum stress of intestinal epithelial cells by blocking Notch signaling pathway
Authors: Liying Dai, Shuangshuang Jie, Shaohua Bi, Qing Qing, Jun Chen
Journal: Bioengineered
PubMed ID: 34565297
Doi: 10.1080/21655979.2021.1985341
A clubroot pathogen effector targets cruciferous cysteine proteases to suppress plant immunity
Authors: Edel Pérez-López, Md Musharaf Hossain, Yangdou Wei, Christopher D Todd, Peta C Bonham-Smith
Journal: Virulence
PubMed ID: 34515618
Doi: 10.1080/21505594.2021.1968684
Products & Services: Mutant Libraries   
Interaction of huntingtin (HTT) with PRMTs and its subsequent arginine methylation affects HTT solubility, phase transition behavior and neuronal toxicity
Authors: Tamara Ratovitski, Mali Jiang, Robert N O'Meally, Priyanka Rauniyar, Ekaterine Chighladze, Anikó Faragó, Siddhi V Kamath, Jing Jin, Alexey V Shevelkin, Robert N Cole, Christopher A Ross
Journal: Hum Mol Genet
PubMed ID: 34888656
Doi: 10.1093/hmg/ddab351
Products & Services: Gene Synthesis   
Impaired Immune Response to COVID-19 Vaccination in Patients with B-Cell Malignancies after CD19 CART Cell Therapy
Authors: Saurabh Dahiya, Tim Luetkens, Forat Lutfi, Stephanie V Avila, Thierry Iraguha, Philip J Margiotta, Kim G Hankey, Patricia Lesho, Jennie Y Law, Seung Tae Lee, John W Baddley, Mehmet Hakan Kocoglu, Jean Yared, Nancy M Hardy, Aaron P Rapoport, Djordje Atanackovic
Journal: Blood Adv
PubMed ID: 34941995
Doi: 10.1182/bloodadvances.2021006112
Products & Services: Gene Synthesis   
Pol IV and RDR2: A two-RNA-polymerase machine that produces double-stranded RNA
Authors: Kun Huang, Xiao-Xian Wu, Cheng-Li Fang, Zhou-Geng Xu, Hong-Wei Zhang, Jian Gao, Chuan-Miao Zhou, Lin-Lin You, Zhan-Xi Gu, Wen-Hui Mu, Yu Feng, Jia-Wei Wang, Yu Zhang
Journal: Science
PubMed ID: 34941388
Products & Services: Gene Synthesis   
Blood virosphere in febrile Tanzanian children
Authors: Samuel Cordey, Florian Laubscher, Mary-Anne Hartley, Thomas Junier, Kristina Keitel, Mylène Docquier, Nicolas Guex, Christian Iseli, Gael Vieille, Philippe Le Mercier, Anne Gleizes, Josephine Samaka, Tarsis Mlaganile, Frank Kagoro, John Masimba, Zamzam Said, Hosiana Temba, Gasser H Elbanna, Caroline Tapparel, Marie-Celine Zanella, Ioannis Xenarios, Jacques Fellay, Valérie D'Acremont, Laurent Kaiser
Journal: Emerg Microbes Infect
PubMed ID: 33929935
Doi: 10.1080/22221751.2021.1925161
Products & Services: Gene Synthesis   
Long-term trajectories of SARS-CoV-2 neutralizing antibodies and predictive value of first dose vaccination-induced IgG-antibodies in hemodialysis patients
Authors: F P Tillmann, H Still, Philipp von Landenberg
Journal: Int Urol Nephrol
PubMed ID: 34860338
Doi: 10.1007/s11255-021-03076-2
Products & Services: Protein Reagents and Products   
Vascular endothelial growth factor-c regulates hematopoietic stem cell fate in the dorsal aorta
Authors: Rebecca K Schiavo, Owen J Tamplin
Journal: Development
PubMed ID: 34919128
Doi: 10.1242/dev.199498
Products & Services: Gene Synthesis   
LncRNA-IRAR-mediated regulation of insulin receptor transcripts in Drosophila melanogaster during nutritional stress
Authors: Jie Chen, Yuantai Huang, Guojun Qi
Journal: Insect Mol Biol
PubMed ID: 34923706
Doi: 10.1111/imb.12756
Products & Services: Gene Synthesis   
MicroRNA miR-188-5p as a mediator of long non-coding RNA MALAT1 regulates cell proliferation and apoptosis in multiple myeloma
Authors: Hui Liu, Zuofei Chi, Hong Jin, Wei Yang
Journal: Bioengineered
PubMed ID: 33944676
Doi: 10.1080/21655979.2021.1920325
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Targeted delivery of a vaccine protein to Langerhans cells in the human skin via the C-type lectin receptor Langerin
Authors: Lydia Bellmann, Helen Strandt, Claudia Zelle-Rieser, Daniela Ortner, Christoph H Tripp, Sandra Schmid, Julia Rühl, Giuseppe Cappellano, Sandra Schaffenrath, Anastasia Prokopi, Sarah Spoeck, Athanasios Seretis, Barbara Del Frari, Stephan Sigl, Johanna Krapf, Christine Heufler, Tibor Keler, Christian Münz, Nikolaus Romani, Patrizia Stoitzner
Journal: Eur J Immunol
PubMed ID: 34932821
Doi: 10.1002/eji.202149670
Products & Services: Plasmid DNA Preparation   
Sustained but Declining Humoral Immunity Against SARS-CoV-2 at 9 Months Postvaccination With BNT162b2: A Prospective Evaluation in 309 Healthy Individuals
Authors: Evangelos Terpos, Vangelis Karalis, Ioannis Ntanasis-Stathopoulos, Filia Apostolakou, Sentiljana Gumeni, Maria Gavriatopoulou, Dimitris Papadopoulos, Panagiotis Malandrakis, Eleni-Dimitra Papanagnou, Eleni Korompoki, Efstathios Kastritis, Ioannis Papassotiriou, Ioannis P Trougakos, Meletios A Dimopoulos
Journal: HemaSphere
PubMed ID: 34938959
Doi: 10.1097/HS9.0000000000000677
Products & Services: Protein Reagents and Products   
Dual-detection fluorescent immunochromatographic assay for quantitative detection of SARS-CoV-2 spike RBD-ACE2 blocking neutralizing antibody
Authors: Xuejun Duan, Yijun Shi, Xudong Zhang, Xiaoxiao Ge, Rong Fan, Jinghan Guo, Yubin Li, Guoge Li, Yaowei Ding, Rasha Alsamani Osman, Wencan Jiang, Jialu Sun, Xin Luan, Guojun Zhang
Journal: Biosens Bioelectron
PubMed ID: 34942543
Doi: 10.1016/j.bios.2021.113883
Products & Services: Protein Reagents and Products   
CryoEM of RUVBL1-RUVBL2-ZNHIT2, a complex that interacts with pre-mRNA-processing-splicing factor 8
Authors: Marina Serna, Ana González-Corpas, Sofía Cabezudo, Andrés López-Perrote, Gianluca Degliesposti, Eduardo Zarzuela, J Mark Skehel, Javier Muñoz, Oscar Llorca
Journal: Nucleic Acids Res
PubMed ID: 34951455
Doi: 10.1093/nar/gkab1267
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Local Charge Distributions, Electric Dipole Moments, and Local Electric Fields Influence Reactivity Patterns and Guide Regioselectivities in α-Ketoglutarate-Dependent Non-heme Iron Dioxygenases
Authors: Sam P de Visser, Gourab Mukherjee, Hafiz Saqib Ali, Chivukula V Sastri
Journal: Acc Chem Res
PubMed ID: 34915695
Doi: 10.1021/acs.accounts.1c00538
Products & Services: Custom Vector Construction   
SARS-CoV-2 Omicron has extensive but incomplete escape of Pfizer BNT162b2 elicited neutralization and requires ACE2 for infection
Authors: Sandile Cele, Laurelle Jackson, David S Khoury, Khadija Khan, Thandeka Moyo-Gwete, Houriiyah Tegally, James Emmanuel San, Deborah Cromer, Cathrine Scheepers, Daniel Amoako, Farina Karim, Mallory Bernstein, Gila Lustig, Derseree Archary, Muneerah Smith, Yashica Ganga, Zesuliwe Jule, Kajal Reedoy, Shi-Hsia Hwa, Jennifer Giandhari, Jonathan M Blackburn, Bernadett I Gosnell, Salim S Abdool Karim, Willem Hanekom, Anne von Gottberg, Jinal Bhiman, Richard J Lessells, Mahomed-Yunus S Moosa, Miles P Davenport, Tulio de Oliveira, Penny L Moore, Alex Sigal
Journal: medrxiv
PubMed ID: 34909788
Doi: 10.1101/2021.12.08.21267417
Products & Services: Monoclonal Antibody Services   
Chlorogenic Acid-Induced Gut Microbiota Improves Metabolic Endotoxemia
Authors: Xiaolin Ye, Yang Liu, Jiajin Hu, Yanyan Gao, Yanan Ma, Deliang Wen
Journal: Front Endocrinol (Lausanne)
PubMed ID: 34975748
Doi: 10.3389/fendo.2021.762691
Products & Services: Endotoxin Detection & Removal System   
Cepharanthine Blocks Presentation of Thyroid and Islet Peptides in a Novel Humanized Autoimmune Diabetes and Thyroiditis Mouse Model
Authors: Cheuk Wun Li, Roman Osman, Francesca Menconi, Larissa C Faustino, Kookjoo Kim, Oliver B Clarke, Hanxi Hou, Yaron Tomer
Journal: Front Immunol
PubMed ID: 34987519
Doi: 10.3389/fimmu.2021.796552
Products & Services: Peptide Synthesis   
Novel Tyrosine Kinase-Mediated Phosphorylation With Dual Specificity Plays a Key Role in the Modulation of Physiology and Virulence
Authors: Sashi Kant, Vijay Pancholi
Journal: Front Microbiol
PubMed ID: 34950110
Doi: 10.3389/fmicb.2021.689246
Ultrasound triggered topical delivery of Bmp7 mRNA for white fat browning induction via engineered smart exosomes
Authors: Yitong Guo, Zhuo Wan, Ping Zhao, Mengying Wei, Yunnan Liu, Te Bu, Wenqi Sun, Zhelong Li, Lijun Yuan
Journal: J Nanobiotechnology
PubMed ID: 34863187
Doi: 10.1186/s12951-021-01145-3
Products & Services: Custom Vector Construction   
PHA-680626 Is an Effective Inhibitor of the Interaction between Aurora-A and N-Myc
Authors: Dalila Boi, Fani Souvalidou, Davide Capelli, Federica Polverino, Grazia Marini, Roberta Montanari, Giorgio Pochetti, Angela Tramonti, Roberto Contestabile, Daniela Trisciuoglio, Patrizia Carpinelli, Camilla Ascanelli, Catherine Lindon, Alessandro De Leo, Michele Saviano, Roberto Di Santo, Roberta Costi, Giulia Guarguaglini, Alessandro Paiardini
Journal: Int J Mol Sci
PubMed ID: 34884931
Doi: 10.3390/ijms222313122
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Antiviral RNA interference in disease vector (Asian longhorned) ticks
Authors: Yan Xu, Zhengwei Zhong, Yanxin Ren, Liting Ma, Zhi Ye, Chuang Gao, Jingwen Wang, Yang Li
Journal: PLoS Pathog
PubMed ID: 34860862
Doi: 10.1371/journal.ppat.1010119
Biosynthesis of the redox cofactor mycofactocin is controlled by the transcriptional regulator MftR and induced by long chain acyl-CoA species
Authors: Aigera Mendauletova, John A Latham
Journal: J Biol Chem
PubMed ID: 34896395
Doi: 10.1016/j.jbc.2021.101474
Products & Services: Custom Vector Construction   
Characterization of recombinant β subunit of human MUC4 mucin (rMUC4β)
Authors: Prakash G Kshirsagar, Mansi Gulati, Wade M Junker, Abhijit Aithal, Gaelle Spagnol, Srustidhar Das, Kavita Mallya, Shailendra K Gautam, Sushil Kumar, Paul Sorgen, Krishan K Pandey, Surinder K Batra, Maneesh Jain
Journal: Sci Rep
PubMed ID: 34887447
Doi: 10.1038/s41598-021-02860-5
Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance
Authors: Yalan Deng, Xianghou Xia, Yang Zhao, Zilong Zhao, Consuelo Martinez, Wenjuan Yin, Jun Yao, Qinglei Hang, Weiche Wu, Jie Zhang, Yang Yu, Weiya Xia, Fan Yao, Di Zhao, Yutong Sun, Haoqiang Ying, Mien-Chie Hung, Li Ma
Journal: Nat Commun
PubMed ID: 34873175
Doi: 10.1038/s41467-021-27349-7
Cockayne Syndrome B Protein Selectively Resolves and Interact with Intermolecular DNA G-Quadruplex Structures
Authors: Denise Liano, Souroprobho Chowdhury, Marco Di Antonio
Journal: J Am Chem Soc
PubMed ID: 34855372
Doi: 10.1021/jacs.1c10745
Transmembrane topology and oligomeric nature of an astrocytic membrane protein, MLC1
Authors: Junmo Hwang, Kunwoong Park, Ga-Young Lee, Bo Young Yoon, Hyunmin Kim, Sung Hoon Roh, Byoung-Cheol Lee, Kipom Kim, Hyun-Ho Lim
Journal: Open Biol
PubMed ID: 34847774
Doi: 10.1098/rsob.210103
Products & Services: Mammalian Expression   
Phosphate-mediated coanchoring of RBD immunogens and molecular adjuvants to alum potentiates humoral immunity against SARS-CoV-2
Authors: Kristen A Rodrigues, Sergio A Rodriguez-Aponte, Neil C Dalvie, Jeong Hyun Lee, Wuhbet Abraham, Diane G Carnathan, Luis E Jimenez, Julia T Ngo, Jason Y H Chang, Zeli Zhang, Jingyou Yu, Aiquan Chang, Catherine Nakao, Benjamin Goodwin, Christopher A Naranjo, Libin Zhang, Murillo Silva, Dan H Barouch, Guido Silvestri, Shane Crotty, J Christopher Love, Darrell J Irvine
Journal: Sci Adv
PubMed ID: 34878851
Doi: 10.1126/sciadv.abj6538
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MicroRNA-934 facilitates cell proliferation, migration, invasion and angiogenesis in colorectal cancer by targeting B-cell translocation gene 2
Authors: Bo Li, Xianyi Liu, Guogang Wu, Jiawen Liu, Shouliang Cai, Fuxin Wang, Chunyu Yang, Jisheng Liu
Journal: Bioengineered
PubMed ID: 34699325
Doi: 10.1080/21655979.2021.1996505
Products & Services: Custom DNA/RNA Oligos   
Cyclic nucleotide-gated ion channel 6 is involved in extracellular ATP signaling and plant immunity
Authors: Ha N Duong, Sung-Hwan Cho, Limin Wang, An Q Pham, Julia M Davies, Gary Stacey
Journal: Plant J
PubMed ID: 34919778
Doi: 10.1111/tpj.15636
Products & Services: Catalog Peptides   
Variation in Severe Acute Respiratory Syndrome Coronavirus 2 Bioaerosol Production in Exhaled Breath
Authors: Renu Verma, Eugene Kim, Nicholas Degner, Katharine S Walter, Upinder Singh, Jason R Andrews
Journal: Open Forum Infect Dis
PubMed ID: 35028332
Doi: 10.1093/ofid/ofab600
Products & Services: Plasmid DNA Preparation   
Protein S-Nitrosylation Regulates Postmortem Beef Apoptosis through the Intrinsic Mitochondrial Pathway
Authors: Qin Hou, Qiongniu Zhu, Wenwei Lu, Wangang Zhang
Journal: J Agric Food Chem
PubMed ID: 34968404
Doi: 10.1021/acs.jafc.1c06516
GST-Perfringolysin O production for the localization and quantification of membrane cholesterol in human and mouse brain and liver
Authors: Leire Goicoechea, Fabian Arenas, Fernanda Castro, Susana Nuñez, Sandra Torres, Carmen Garcia-Ruiz, José C Fernandez-Checa
Journal: STAR Protoc
PubMed ID: 35024626
Doi: 10.1016/j.xpro.2021.101068
Products & Services: Custom DNA/RNA Oligos   
CTLA4-Ig treatment induces M1-M2 shift in cultured monocyte-derived macrophages from healthy subjects and rheumatoid arthritis patients
Authors: Maurizio Cutolo, Stefano Soldano, Emanuele Gotelli, Paola Montagna, Rosanna Campitiello, Sabrina Paolino, Carmen Pizzorni, Alberto Sulli, Vanessa Smith, Samuele Tardito
Journal: Arthritis Res Ther
PubMed ID: 34952630
Doi: 10.1186/s13075-021-02691-9
The microbial gbu gene cluster links cardiovascular disease risk associated with red meat consumption to microbiota L-carnitine catabolism
Authors: Jennifer A Buffa, Kymberleigh A Romano, Matthew F Copeland, David B Cody, Weifei Zhu, Rachel Galvez, Xiaoming Fu, Kathryn Ward, Marc Ferrell, Hong J Dai, Sarah Skye, Ping Hu, Lin Li, Mirjana Parlov, Amy McMillan, Xingtao Wei, Ina Nemet, Robert A Koeth, Xinmin S Li, Zeneng Wang, Naseer Sangwan, Adeline M Hajjar, Mohammed Dwidar, Taylor L Weeks, Nathalie Bergeron, Ronald M Krauss, W H Wilson Tang, Federico E Rey, Joseph A DiDonato, Valentin Gogonea, G Frank Gerberick, Jose Carlos Garcia-Garcia, Stanley L Hazen
Journal: Nat Microbiol
PubMed ID: 34949826
Doi: 10.1038/s41564-021-01010-x
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Cryo-EM structure of constitutively active human Frizzled 7 in complex with heterotrimeric Gs
Authors: Lu Xu, Bo Chen, Hannes Schihada, Shane C Wright, Ainoleena Turku, Yiran Wu, Gye-Won Han, Maria Kowalski-Jahn, Pawel Kozielewicz, Carl-Fredrik Bowin, Xianjun Zhang, Chao Li, Michel Bouvier, Gunnar Schulte, Fei Xu
Journal: cell research
PubMed ID: 34239071
Doi: 10.1038/s41422-021-00525-6
Omicron extensively but incompletely escapes Pfizer BNT162b2 neutralization
Authors: Sandile Cele, Laurelle Jackson, David S Khoury, Khadija Khan, Thandeka Moyo-Gwete, Houriiyah Tegally, James Emmanuel San, Deborah Cromer, Cathrine Scheepers, Daniel G Amoako, Farina Karim, Mallory Bernstein, Gila Lustig, Derseree Archary, Muneerah Smith, Yashica Ganga, Zesuliwe Jule, Kajal Reedoy, Shi-Hsia Hwa, Jennifer Giandhari, Jonathan M Blackburn, Bernadett I Gosnell, Salim S Abdool Karim, Willem Hanekom, Anne von Gottberg, Jinal N Bhiman, Richard J Lessells, Mahomed-Yunus S Moosa, Miles P Davenport, Tulio de Oliveira, Penny L Moore, Alex Sigal
Journal: Nature
PubMed ID: 35016196
Doi: 10.1038/s41586-021-04387-1
Products & Services: Monoclonal Antibody Services   
Modulation of anti-angiogenic activity using ultrasound-activated nutlin-loaded piezoelectric nanovectors
Authors: Özlem Şen, Attilio Marino, Carlotta Pucci, Gianni Ciofani
Journal: Mater Today Bio
PubMed ID: 35005600
Doi: 10.1016/j.mtbio.2021.100196
Products & Services: GenParts™ DNA Fragments   
A human cell-based SARS-CoV-2 vaccine elicits potent neutralizing antibody responses and protects mice from SARS-CoV-2 challenge
Authors: Xiangchuan He, Longfei Ding, Kangli Cao, Haoran Peng, Chenjian Gu, Yutang Li, Duoduo Li, Lanlan Dong, Xiujing Hong, Xiangwei Wang, Meilan Fu, Chenli Qiu, Cuisong Zhu, Ziling Zhang, Shu Song, Chenguang Wang, Zhengfan Jiang, Youhua Xie, Zhongtian Qi, Chen Zhao, Ping Zhao, Xiaoyan Zhang, Jianqing Xu
Journal: Emerg Microbes Infect
PubMed ID: 34304724
Doi: 10.1080/22221751.2021.1957400
Products & Services: Recombinant Antibody Expression   
Heterologous prime-boost immunizations with chimpanzee adenoviral vectors elicit potent and protective immunity against SARS-CoV-2 infection
Authors: Jiaojiao Liu, Kun Xu, Man Xing, Yue Zhuo, Jingao Guo, Meng Du, Qi Wang, Yaling An, Jinhe Li, Ping Gao, Yihan Wang, Furong He, Yingying Guo, Mingxi Li, Yuchao Zhang, Linqi Zhang, George F Gao, Lianpan Dai, Dongming Zhou
Journal: Cell Discov
PubMed ID: 34923570
Doi: 10.1038/s41421-021-00360-4
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Platelet Endothelial Cell Adhesion Molecule 1 (CD31) Is Essential for Beta-Toxin Mediated Cytotoxicity in Human Endothelial and Monocytic Cells
Authors: Basma Tarek, Julia Bruggisser, Filippo Cattalani, Horst Posthaus
Journal: Toxins (Basel)
PubMed ID: 34941730
Doi: 10.3390/toxins13120893
Products & Services: Recombinant Antibody Expression   
β-galactosidase GALA from with high transgalactosylation activity
Authors: Yaru Yan, Weishi Guan, Xiaoyi Li, Kaier Gao, Xinxin Xu, Bo Liu, Wei Zhang, Yuhong Zhang
Journal: Bioengineered
PubMed ID: 34606421
Doi: 10.1080/21655979.2021.1988370
Products & Services: Plasmid DNA Preparation   
Small molecule splicing modifiers with systemic HTT-lowering activity
Authors: Anuradha Bhattacharyya, Christopher R Trotta, Jana Narasimhan, Kari J Wiedinger, Wencheng Li, Kerstin A Effenberger, Matthew G Woll, Minakshi B Jani, Nicole Risher, Shirley Yeh, Yaofeng Cheng, Nadiya Sydorenko, Young-Choon Moon, Gary M Karp, Marla Weetall, Amal Dakka, Vijayalakshmi Gabbeta, Nikolai A Naryshkin, Jason D Graci, Thomas Tripodi, Amber Southwell, Michael Hayden, Joseph M Colacino, Stuart W Peltz
Journal: Nat Commun
PubMed ID: 34911927
Doi: 10.1038/s41467-021-27157-z
Products & Services: Gene Synthesis   
Curcumin modulates airway remodelling-contributing genes-the significance of transcription factors
Authors: Joanna Wieczfinska, Przemysław Sitarek, Tomasz Kowalczyk, Piotr Rieske, Rafal Pawliczak
Journal: J Cell Mol Med
PubMed ID: 34939316
Doi: 10.1111/jcmm.17102
Development of a high-throughput RT-PCR based viral infectivity assay for monitoring the stability of a replicating recombinant Lymphocytic Choriomeningitis viral vector
Authors: Vineet Gupta, Lorena R Antunez, Soraia Saleh-Birdjandi, Ozan S Kumru, Richard Pospisil, Anders Lilja, Gerhard Fuhrmann, Lee Smith, David B Volkin, Sangeeta B Joshi
Journal: J Virol Methods
PubMed ID: 34954306
Doi: 10.1016/j.jviromet.2021.114440
MiR-31 targets HSD17B14 and FSHR, and miR-20b targets HSD17B14 to affect apoptosis and steroid hormone metabolism of porcine ovarian granulosa cells
Authors: Siyuan Gao, Jing Zhao, Qinglei Xu, Yanli Guo, Mingzheng Liu, Chunlei Zhang, Allan P Schinckel, Bo Zhou
Journal: Theriogenology
PubMed ID: 34959084
Doi: 10.1016/j.theriogenology.2021.12.014
Djulis Hull Improves Insulin Resistance and Modulates the Gut Microbiota in High-Fat Diet (HFD)-Induced Hyperglycaemia
Authors: Yu-Tang Tung, Jun-Lan Zeng, Shang-Tse Ho, Jin-Wei Xu, I-Hsuan Lin, Jyh-Horng Wu
Journal: Antioxidants (Basel)
PubMed ID: 35052549
Doi: 10.3390/antiox11010045
Products & Services: Endotoxin Detection & Removal System   
In vivo architecture of the telomerase RNA catalytic core in Trypanosoma brucei
Authors: Abhishek Dey, Anais Monroy-Eklund, Kaitlin Klotz, Arpita Saha, Justin Davis, Bibo Li, Alain Laederach, Kausik Chakrabarti
Journal: Nucleic Acids Res
PubMed ID: 34850114
Doi: 10.1093/nar/gkab1042
Products & Services: Catalog Antibody   
Species-selective targeting of pathogens revealed by the atypical structure and active site of Trypanosoma cruzi histone deacetylase DAC2
Authors: Martin Marek, Elizabeth Ramos-Morales, Gisele F A Picchi-Constante, Theresa Bayer, Carina Norström, Daniel Herp, Policarpo A Sales-Junior, Eloise P Guerra-Slompo, Kristin Hausmann, Alokta Chakrabarti, Tajith B Shaik, Annika Merz, Edouard Troesch, Karin Schmidtkunz, Samuel Goldenberg, Raymond J Pierce, Marina M Mourão, Manfred Jung, Johan Schultz, Wolfgang Sippl, Nilson I T Zanchin, Christophe Romier
Journal: Cell Rep
PubMed ID: 34936867
Doi: 10.1016/j.celrep.2021.110129
Products & Services: Plasmid DNA Preparation   
Multiple RNA virus matrix proteins interact with SLD5 to manipulate host cell cycle
Authors: Li Zhu, Xinyu Li, Henan Xu, Lifeng Fu, George Fu Gao, Wenjun Liu, Linqing Zhao, Xiaojun Wang, Wei Jiang, Min Fang
Journal: J Gen Virol
PubMed ID: 34882534
Doi: 10.1099/jgv.0.001697
Products & Services: Polyclonal Antibody Services   
ICAM-1 Targeted Drug Combination Nanoparticles Enhanced Gemcitabine-Paclitaxel Exposure and Breast Cancer Suppression in Mouse Models
Authors: Linxi Zhu, Qingxin Mu, Jesse Yu, James I Griffin, Xiaolin Xu, Rodney J Y Ho
Journal: Pharmaceutics
PubMed ID: 35056985
Doi: 10.3390/pharmaceutics14010089
Products & Services: Gene Synthesis   
Comparative analysis of mussel foot protein 3B co-expressed with tyrosinases provides a potential adhesive biomaterial
Authors: Lin Yao, Xinyi Wang, Rui Xue, Hong Xu, Rui Wang, Lujia Zhang, Sha Li
Journal: Int J Biol Macromol
PubMed ID: 34896153
Doi: 10.1016/j.ijbiomac.2021.11.208
Products & Services: Bacterial Expression   
Key factors determining competitions between double-stranded RNAs in Tribolium castaneum
Authors: Xu Shen, Yingchuan Peng, Huifang Song, Jinda Wang, Jun Zhao, Peian Tang, Zhaojun Han, Kangxu Wang
Journal: Pestic Biochem Physiol
PubMed ID: 35082032
Doi: 10.1016/j.pestbp.2021.105009
Products & Services: Gene Synthesis   

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