客户发表文献

论文在线提交

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

开始时间
页码: 64/1027 每页显示数量
Spatiotemporal and global profiling of DNA–protein interactions enables discovery of low-affinity transcription factors
Authors: An-Di Guo , Ke-Nian Yan, Hao Hu , Linhui Zhai , Teng-Fei Hu , Haixia Su , Yijia Chi , Jinyin Zha , Yechun Xu , Dongxin Zhao , Xiaojie Lu , Yong-Jiang Xu , Jian Zhang , Minjia Tan, Xiao-Hua Chen
Journal: Nat Chem .
PubMed ID: 37106095
Doi: 10.1038/s41557-023-01196-z
Products & Services: Peptide Synthesis   
Homology-independent targeted insertion (HITI) enables guided CAR knock-in and efficient clinical scale CAR-T cell manufacturing
Authors: Hyatt Balke-Want, Vimal Keerthi, Nikolaos Gkitsas, Andrew G Mancini, Gavin L Kurgan, Carley Fowler, Peng Xu, Xikun Liu, Kyle Asano, Sunny Patel, Christopher J Fisher, Annie K Brown, Ramya H Tunuguntla, Shabnum Patel, Elena Sotillo, Crystal L Mackall, Steven A Feldman
Journal: Mol Cancer
PubMed ID: 37365642
Doi: 10.1186/s12943-023-01799-7
Genome-wide characterization of extrachromosomal circular DNA in gastric cancer and its potential role in carcinogenesis and cancer progression
Authors: Xianming Jiang, Xiaoguang Pan, Wenchao Li, Peng Han, Jiaying Yu, Jing Li, Haoran Zhang, Wei Lv, Ying Zhang, Yulong He, Xi Xiang
Journal: Cell Mol Life Sci
PubMed ID: 37369919
Doi: 10.1007/s00018-023-04838-0
CLDN182 and 4-1BB bispecific antibody givastomig exerts antitumor activity through CLDN182-expressing tumor-directed T-cell activation
Authors: Jing Gao, Zhengyi Wang, Wenqing Jiang, Yanni Zhang, Zhen Meng, Yanling Niu, Zhen Sheng, Chan Chen, Xuejun Liu, Xi Chen, Chanjuan Liu, Keren Jia, Cheng Zhang, Haiyan Liao, Jaeho Jung, Eunsil Sung, Hyejin Chung, Jingwu Z Zhang, Andrew X Zhu, Lin Shen
Journal: J Immunother Cancer
PubMed ID: 37364935
Doi: 10.1136/jitc-2023-006704
ENPP1 is an innate immune checkpoint of the anticancer cGAMP-STING pathway
Authors: Songnan Wang, Volker Böhnert, Alby J Joseph, Valentino Sudaryo, Jason Swinderman, Feiqiao B Yu, Xuchao Lyu, Gemini Skariah, Vishvak Subramanyam, Luke A Gilbert, Hani Goodarzi, Li Lingyin
Journal: biorxiv
PubMed ID: 37333273
Doi: 10.1101/2023.06.01.543353
Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-Müllerian hormone transgene
Authors: Lindsey M Vansandt, Marie-Charlotte Meinsohn, Philippe Godin, Nicholas Nagykery, Natalie Sicher, Motohiro Kano, Aki Kashiwagi, Maeva Chauvin, Hatice D Saatcioglu, Julie L Barnes, Amy G Miller, Amy K Thompson, Helen L Bateman, Elizabeth M Donelan, Raquel González, Jackie Newsom, Guangping Gao, Patricia K Donahoe, Dan Wang, William F Swanson, David Pépin
Journal: Nat Commun
PubMed ID: 37280258
Doi: 10.1038/s41467-023-38721-0
Products & Services: Codon Optimization   
Novel SPEA Superantigen Peptide Agonists and Peptide Agonist-TGFαL3 Conjugate In Vitro Study of Their Growth-Inhibitory Effects for Targeted Cancer Immunotherapy
Authors: Sara S Bashraheel, Sayed K Goda
Journal: Int J Mol Sci
PubMed ID: 37445686
Doi: 10.3390/ijms241310507
Products & Services: Peptide Synthesis   
Development of MDCK-based quadrivalent split seasonal influenza virus vaccine with high safety and immunoprotection: A preclinical study
Authors: Jiayou Zhang, Xuanxuan Nian, Bo Liu, Zhegang Zhang, Wei Zhao, Xixin Han, Yumei Ma, Dongwu Jin, Hua Ma, Qingmei Zhang, Ran Qiu, Fang Li, Zheng Gong, Xuedan Li, Ying Yang, Yichao Tian, Li Zhou, Kai Duan, Xinguo Li, Zhongren Ma, Xiaoming Yang
Journal: Antiviral Res
PubMed ID: 37270159
Doi: 10.1016/j.antiviral.2023.105639
Products & Services: Molecular Biology Reagents   
Intact mass analysis reveals the novel O-linked glycosylation on the stalk region of PD-1 protein
Authors: Phanthakarn Tit-Oon, Arisa Wonglangka, Klaichan Boonkanta, Mathuros Ruchirawat, Mayuree Fuangthong, Ram Sasisekharan, Amnart Khongmanee
Journal: Sci Rep
PubMed ID: 37316505
Doi: 10.1038/s41598-023-36203-3
Products & Services: Molecular Biology Reagents   
Development of allosteric and selective CDK2 inhibitors for contraception with negative cooperativity to cyclin binding
Authors: Erik B Faber, Luxin Sun, Jian Tang, Emily Roberts, Sornakala Ganeshkumar, Nan Wang, Damien Rasmussen, Abir Majumdar, Laura E Hirsch, Kristen John, An Yang, Hira Khalid, Jon E Hawkinson, Nicholas M Levinson, Vargheese Chennathukuzhi, Daniel A Harki, Ernst Schönbrunn, Gunda I Georg
Journal: Nat Commun
PubMed ID: 37270540
Doi: 10.1038/s41467-023-38732-x
Products & Services: Molecular Biology Reagents   
Grafting Rhodobacter sphaeroides with red algae Rubisco to accelerate catalysis and plant growth
Authors: Yu Zhou, Laura H Gunn, Rosemary Birch, Inger Andersson, Spencer M Whitney
Journal: Nat Plants
PubMed ID: 37291398
Doi: 10.1038/s41477-023-01436-7
Products & Services: Molecular Biology Reagents   
Systematic Mining and Evaluation of the Sesquiterpene Skeletons as High Energy Aviation Fuel Molecules
Authors: Yanglei Huang, Ziling Ye, Xiukun Wan, Ge Yao, Jingyu Duan, Jiajia Liu, Mingdong Yao, Xiang Sun, Zixin Deng, Kun Shen, Hui Jiang, Tiangang Liu
Journal: Adv Sci (Weinh)
PubMed ID: 37271925
Doi: 10.1002/advs.202300889
Products & Services: Molecular Biology Reagents   
Glutamine synthetase (GS) knockout (KO) using CRISPR/Cpf1 diversely enhances selection efficiency of CHO cells expressing therapeutic antibodies
Authors: Witsanu Srila, Martina Baumann, Markus Riedl, Kuntalee Rangnoi, Nicole Borth, Montarop Yamabhai
Journal: Sci Rep
PubMed ID: 37380701
Doi: 10.1038/s41598-023-37288-6
An Inferred Ancestral CotA Laccase with Improved Expression and Kinetic Efficiency
Authors: Lei Lei, Lijun Zhao, Yiqia Hou, Chen Yue, Pulin Liu, Yanli Zheng, Wenfang Peng, Jiangke Yang
Journal: Int J Mol Sci
PubMed ID: 37446078
Doi: 10.3390/ijms241310901
Products & Services: Recombinant Proteins   
Author Correction: RNA conformational propensities determine cellular activity
Authors: Megan L Ken, Rohit Roy, Ainan Geng, Laura R Ganser, Akanksha Manghrani, Bryan R Cullen, Ursula Schulze-Gahmen, Daniel Herschlag, Hashim M Al-Hashimi
Journal: Nature
PubMed ID: 37296211
Doi: 10.1038/s41586-023-06286-z
Products & Services: Peptide Synthesis   
Combined lineage tracing and scRNA-seq reveals unexpected first heart field predominance of human iPSC differentiation
Authors: Francisco X Galdos, Carissa Lee, Soah Lee, Sharon Paige, William Goodyer, Sidra Xu, Tahmina Samad, Gabriela V Escobar, Adrija Darsha, Aimee Beck, Rasmus O Bak, Matthew H Porteus, Sean M Wu
Journal: Elife
PubMed ID: 37284748
Doi: 10.7554/eLife.80075
Products & Services: Molecular Biology Reagents   
The role of mechano-regulated YAP/TAZ in erectile dysfunction
Authors: Mintao Ji, Dongsheng Chen, Yinyin Shu, Shuai Dong, Zhisen Zhang, Haimeng Zheng, Xiaoni Jin, Lijun Zheng, Yang Liu, Yifei Zheng, Wensheng Zhang, Shiyou Wang, Guangming Zhou, Bingyan Li, Baohua Ji, Yong Yang, Yongde Xu, Lei Chang
Journal: Nat Commun
PubMed ID: 37353497
Doi: 10.1038/s41467-023-39009-z
Odorant-binding protein 19 in Monochamus alternatus involved in the recognition of a volatile strongly emitted from ovipositing host pines
Authors: Ruinan Yang, Dongzhen Li, Shancheng Yi, Yi Wei, Manqun Wang
Journal: Insect Sci
PubMed ID: 37358042
Doi: 10.1111/1744-7917.13238
Products & Services: Catalog Antibody   
The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins
Authors: Julie Lavie, Claude Lalou, Walid Mahfouf, Jean-William Dupuy, Aurélie Lacaule, Agata Ars Cywinska, Didier Lacombe, Anne-Marie Duchêne, Anne-Aurélie Raymond, Hamid Reza Rezvani, Richard Patryk Ngondo, Giovanni Bénard
Journal: Cell Rep
PubMed ID: 37267103
Doi: 10.1016/j.celrep.2023.112579
Products & Services: Catalog Peptides   
Modularity and diversity of target selectors in Tn7 transposons
Authors: Guilhem Faure, Makoto Saito, Sean Benler, Iris Peng, Yuri I Wolf, Jonathan Strecker, Han Altae-Tran, Edwin Neumann, David Li, Kira S Makarova, Rhiannon K Macrae, Eugene V Koonin, Feng Zhang
Journal: Mol Cell
PubMed ID: 37267947
Doi: 10.1016/j.molcel.2023.05.013
Products & Services: Molecular Biology Reagents   
Performance of commercial SARS-CoV-2 wild-type and Omicron BA1 antibody assays compared with pseudovirus neutralization tests
Authors: E Habermann, L M Frommert, K Ghannam, L Nguyen My, L Gieselmann, P Tober-Lau, J Klotsche, A N Arumahandi de Silva, A Ten Hagen, J Zernicke, F Kurth, L E Sander, F Klein, G R Burmester, R Biesen, F N Albach
Journal: J Clin Virol
PubMed ID: 37354690
Doi: 10.1016/j.jcv.2023.105518
Negatively charged, intrinsically disordered regions can accelerate target search by DNA-binding proteins
Authors: Xi Wang, Lavi S Bigman, Harry M Greenblatt, Binhan Yu, Yaakov Levy, Junji Iwahara
Journal: Nucleic Acids Res
PubMed ID: 36774964
Doi: 10.1093/nar/gkad045
Products & Services: Molecular Biology Reagents   
Circ_PIAS1 Promotes the Apoptosis of ALV-J Infected DF1 Cells by Up-Regulating miR-183
Authors: Ting Yang , Lingling Qiu , Shihao Chen , Zhixiu Wang , Yong Jiang , Hao Bai , Yulin Bi , Guohong Chen , Guobin Chang
Journal: Genes (Basel) .
PubMed ID: 37372440
Doi: 10.3390/genes14061260
Broad Sarbecovirus Neutralizing Antibodies Obtained by Computational Design and Synthetic Library Screening
Authors: Xuehua Yang, Huarui Duan, Xiuying Liu, Xinhui Zhang, Shengnan Pan, Fangyuan Zhang, Peixiang Gao, Bo Liu, Jian Yang, Xiaojing Chi, Wei Yang
Journal: J Virol
PubMed ID: 37367229
Doi: 10.1128/jvi.00610-23
Restriction of SARS-CoV-2 replication by receptor transporter protein 4 (RTP4)
Authors: Ramanjit Kaur, Takuya Tada, Nathaniel Roy Landau
Journal: MBio
PubMed ID: 37382452
Doi: 10.1128/mbio.01090-23
Products & Services: Molecular Biology Reagents   
Inhibition of chitin deacetylases to attenuate plant fungal diseases
Authors: Lin Liu, Yeqiang Xia, Yingchen Li, Yong Zhou, Xiaofeng Su, Xiaojing Yan, Yan Wang, Wende Liu, Hongmei Cheng, Yuanchao Wang, Qing Yang
Journal: Nat Commun
PubMed ID: 37385996
Doi: 10.1038/s41467-023-39562-7
Products & Services: Gene Synthesis   
Structural basis for the binding of DNP and purine nucleotides onto UCP1
Authors: Yunlu Kang, Lei Chen
Journal: Nature
PubMed ID: 37336486
Doi: 10.1038/s41586-023-06332-w
Products & Services: Gene Synthesis   
Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availability
Authors: Anil Kumar Vijjamarri, Xiao Niu, Matthew D Vandermeulen, Chisom Onu, Fan Zhang, Hongfang Qiu, Neha Gupta, Swati Gaikwad, Miriam L Greenberg, Paul J Cullen, Zhenguo Lin, Alan G Hinnebusch
Journal: Elife
PubMed ID: 37266577
Doi: 10.7554/eLife.85545
Products & Services: PCR Cloning and Subcloning   
Complementary methods for monitoring hole-hole homodimer associated with a WuXiBody-based asymmetric bispecific antibody
Authors: Wanyuan Dong, Yifeng Li
Journal: Protein Expr Purif
PubMed ID: 37271410
Doi: 10.1016/j.pep.2023.106316
Chimeric antigen receptor T cells targeting FcRH5 provide robust tumour-specific responses in murine xenograft models of multiple myeloma
Authors: Dongpeng Jiang, Haiwen Huang, Huimin Qin, Koukou Tang, Xiangru Shi, Tingting Zhu, Yuqing Gao, Ying Zhang, Xiaopeng Tian, Jianhong Fu, Weiwei Qu, Weilan Cai, Yang Xu, Depei Wu, Jianhong Chu
Journal: Nat Commun
PubMed ID: 37339964
Doi: 10.1038/s41467-023-39395-4
Products & Services: Recombinant Proteins   
KRT10 plays an important role in the release of viral genome from endosomes during H9N2 subtype AIV replication in HeLa cells
Authors: Xiangyu Huang, Guihu Yin, Bin Zhou, Yiqin Cai, Jianing Hu, Jingwen Huang, Zili Chen, Qingtao Liu, Xiuli Feng
Journal: Vet Microbiol
PubMed ID: 37406407
Doi: 10.1016/j.vetmic.2023.109824
Cytoplasmic aggregation of mutant FUS causes multistep RNA splicing perturbations in the course of motor neuron pathology
Authors: Alexander P Rezvykh, Aleksey A Ustyugov, Kirill D Chaprov, Ekaterina V Teterina, Vladimir O Nebogatikov, Daria S Spasskaya, Michael B Evgen'ev, Alexey V Morozov, Sergei Yu Funikov
Journal: Nucleic Acids Res
PubMed ID: 37115004
Doi: 10.1093/nar/gkad319
Schisandra extract ameliorates arthritis pathogenesis by suppressing the NF-κB and MAPK signalling pathways
Authors: Seong Jae Han, Hyemi Lee, Jiho Nam, Cheol-Ho Pan, Jimin Jeon, Siyoung Yang
Journal: J Cell Mol Med
PubMed ID: 37337779
Doi: 10.1111/jcmm.17814
Effects of the deletion and substitution of thioesterase on bacillomycin D synthesis
Authors: Ping Zhang, Ziyan Lv, Zhaoxin Lu, Wenjie Ma, Xiaomei Bie
Journal: Biotechnol Lett
PubMed ID: 37266877
Doi: 10.1007/s10529-023-03373-z
The putative role of the epipeptide EpeX in intra-species competition
Authors: Margarita Kalamara, James Abbott, Tetyana Sukhodub, Cait MacPhee, Nicola R Stanley-Wall
Journal: Microbiology (Reading)
PubMed ID: 37289492
Doi: 10.1099/mic.0.001344
Acentrosomal spindles assemble from branching microtubule nucleation near chromosomes in Xenopus laevis egg extract
Authors: Bernardo Gouveia, Sagar U Setru, Matthew R King, Aaron Hamlin, Howard A Stone, Joshua W Shaevitz, Sabine Petry
Journal: Nat Commun
PubMed ID: 37344488
Doi: 10.1038/s41467-023-39041-z
Epigenetic Iinsights into the Senescence of Porcine Fetal Fibroblasts induced by Passaging
Authors: Jiaying Bi, Dayu Wang, Fuquan Zhu, Xinyue Lu, Yan Xie, Huijun Liu, Meixia Wang, Xu He, Yuan Jiang, Ke Liu, Mingyue Zhao, Tingzhang Wang, Juan Li
Journal: Cell Cycle
PubMed ID: 37338871
Doi: 10.1080/15384101.2023.2222521
Products & Services: Molecular Biology Reagents   
Engineering BioBricks for Deoxysugar Biosynthesis and Generation of New Tetracenomycins
Authors: Heli Tirkkonen, Katelyn V. Brown, Magdalena Niemczura, Zélie Faudemer, Courtney Brown, Larissa V. Ponomareva, Yosra A. Helmy, Jon S. Thorson, S. Eric Nybo, Mikko Metsä-Ketelä, and Khaled A. Shaaban
Journal: ACS Omega
Doi: 10.1021/acsomega.3c02460
Products & Services: Gene Synthesis   PCR Cloning and Subcloning   
Structural insights into neurokinin 3 receptor activation by endogenous and analogue peptide agonists
Authors: Wenjing Sun , Fan Yang , Huanhuan Zhang , Qingning Yuan , Shenglong Ling , Yuanxia Wang , Pei Lv , Zelin Li , Yifan Luo , Dongsheng Liu , Wanchao Yin , Pan Shi , H Eric Xu, Changlin Tian
Journal: Cell Discov .
PubMed ID: 37391393
Doi: 10.1038/s41421-023-00564-w
Products & Services: Peptide Synthesis   
Structural and biochemical insights into the molecular mechanism of TRPT1 for nucleic acid ADP-ribosylation
Authors: Xiaoyun Yang , Jiaxu Wang, Simin Li , Xiaobing Li , Jingjing Gong , Zhenzhen Yan , Huan Zhou , Chen Wu , Xiuhua Liu
Journal: Nucleic Acids Res .
PubMed ID: 37334830
Doi: 10.1093/nar/gkad525
Products & Services: Codon Optimization   
Molecular Quantification and Mutation Detection as Essential Tools for the Clinical Management of Chronic Myeloid Leukemia Patients: Results from a Brazilian Single-Center Study
Authors: Anelis Maria Marin, Denise Kusma Wosniaki, Heloisa Bruna Soligo Sanchuki, Eduardo Cilião Munhoz, Jeanine Marie Nardin, Gabriela Silva Soares, Dhienifer Caroline Espinace, João Samuel de Holanda Farias, Bruna Veroneze, Luiz Felipe Becker, Guilherme Lima Costa, Olair Carlos Beltrame, Jaqueline Carvalho de Oliveira, Geison Cambri, Dalila Luciola Zanette, Mateus Nóbrega Aoki
Journal: Int J Mol Sci
PubMed ID: 37373266
Doi: 10.3390/ijms241210118
Products & Services: Molecular Biology Reagents   
Isolation of recombinant apolipoprotein E4 N-terminal domain by foam fractionation
Authors: Kyle Lethcoe, Colin A Fox, Anouar Hafiane, Robert S Kiss, Robert O Ryan
Journal: Protein Expr Purif
PubMed ID: 37290717
Doi: 10.1016/j.pep.2023.106319
Products & Services: Molecular Biology Reagents   
Clostridium perfringens epsilon prototoxin mutant rpETX as a vaccine candidate against enterotoxemia
Authors: Jige Du, Tuanjie Wang, Lei Xu, Cong Wang, Ying Liu, Chenfan Pan, Xiaoyun Chen, Zhen Zhu, Yufeng Luo, Chunsheng Yin
Journal: Vaccine
PubMed ID: 37357076
Doi: 10.1016/j.vaccine.2023.06.044
Products & Services: Molecular Biology Reagents   
Poly(A)-binding protein is an ataxin-2 chaperone that regulates biomolecular condensates
Authors: Steven Boeynaems, Yanniv Dorone, Yanrong Zhuang, Victoria Shabardina, Guozhong Huang, Anca Marian, Garam Kim, Anushka Sanyal, Nesli-Ece Şen, Daniel Griffith, Roberto Docampo, Keren Lasker, Iñaki Ruiz-Trillo, Georg Auburger, Alex S Holehouse, Edor Kabashi, Yi Lin, Aaron D Gitler
Journal: Mol Cell
PubMed ID: 37295429
Doi: 10.1016/j.molcel.2023.05.025
Products & Services: Molecular Biology Reagents   
MitoNEET prevents iron overload-induced insulin resistance in H9c2 cells through regulation of mitochondrial iron
Authors: Eddie Tam, Hye K Sung, Gary Sweeney
Journal: J Cell Physiol
PubMed ID: 37269467
Doi: 10.1002/jcp.31044
Products & Services: Molecular Biology Reagents   
Revealing the impact of CD70 expression on the manufacture and functions of CAR-70 T-cells based on single-cell transcriptomics
Authors: Jiali Cheng, Yuyan Zhao, Hui Hu, Ling Tang, Yuhao Zeng, Xinyue Deng, Shengnan Ding, An-Yuan Guo, Qing Li, Xiaojian Zhu
Journal: Cancer Immunol Immunother
PubMed ID: 37382633
Doi: 10.1007/s00262-023-03475-7
Comparative assessment of homemade ELISA and lateral flow assay (LFA)in the rapid, specific and sensitive detection of SARS-CoV-2 anti-nucleocapsid protein in sera of Egyptian patients
Authors: Asmaa El-Shershaby, Nahla Hussein, Esraa Ali, Amr El-Hakim, Ashraf Tabll, Mohamed Shaheen, Ibrahim Ali, Mahmoud Elshall, Yasser Shahein
Journal: J Immunoassay Immunochem
PubMed ID: 37319429
Doi: 10.1080/15321819.2023.2224865
Products & Services: Molecular Biology Reagents   
SpyTag/SpyCatcher display of influenza M2e peptide on norovirus-like particle provides stronger immunization than direct genetic fusion
Authors: Vili Lampinen, Stina Gröhn, Saana Soppela, Vesna Blazevic, Vesa P Hytönen, Minna M Hankaniemi
Journal: Front Cell Infect Microbiol
PubMed ID: 37424789
Doi: 10.3389/fcimb.2023.1216364
Products & Services: Peptide Synthesis   
Recombinant Bacillus Calmette-Guérin Expressing SARS-CoV-2 Chimeric Protein Protects K18-hACE2 Mice against Viral Challenge
Authors: Fábio Mambelli, Fábio V Marinho, Juvana M Andrade, Ana C V S C de Araujo, Rodrigo P F Abuna, Victor M R Fabri, Bruno P O Santos, João S da Silva, Mariana T Q de Magalhães, E Jane Homan, Luciana C C Leite, Greicy B M Dias, Nicoli Heck, Daniel A G B Mendes, Daniel S Mansur, André Báfica, Sergio C Oliveira
Journal: J Immunol
PubMed ID: 37098890
Doi: 10.4049/jimmunol.2200731
Products & Services: Peptide Synthesis   
A MYB activator, DcMYB11c, regulates carrot anthocyanins accumulation in petiole but not taproot
Authors: Ao-Qi Duan, Yuan-Jie Deng, Shan-Shan Tan, Zhi-Sheng Xu, Ai-Sheng Xiong
Journal: Plant Cell Environ
PubMed ID: 37338208
Doi: 10.1111/pce.14653
Products & Services: PCR Cloning and Subcloning   

喜欢新升级的网站吗?

讨厌

不喜欢

一般

喜欢

非常喜欢

*