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Development of a novel ultrasound- and biocrosslinking-enhanced immobilization strategy with application to food enzymes

Authors: Juan Han , Ting Zhang , Zhuoyue Zhou , Houjin Zhang

Journal: Food Chem .

PubMed ID: 36917903

Doi: 10.1016/j.foodchem.2023.135810

Products & Services: Proteins, Expression, Isolation and Analysis

Publisher Correction: High-throughput characterization of HLA-E-presented CD94/NKG2x ligands reveals peptides which modulate NK cell activation

Authors: Brooke D Huisman, Ning Guan, Timo Rückert, Lee Garner, Nishant K Singh, Andrew J McMichael, Geraldine M Gillespie, Chiara Romagnani, Michael E Birnbaum

Journal: Nat Commun

PubMed ID: 37644057

Doi: 10.1038/s41467-023-40946-y

Products & Services: Peptide Synthesis

Microbe-induced gene silencing boosts crop protection against soil-borne fungal pathogens

Authors: Han-Guang Wen, Jian-Hua Zhao, Bo-Sen Zhang, Feng Gao, Xue-Ming Wu, Yong-Sheng Yan, Jie Zhang, Hui-Shan Guo

Journal: Nat Plants

PubMed ID: 37653339

Doi: 10.1038/s41477-023-01507-9

Products & Services: Custom DNA/RNA Oligos

Effectiveness of Bivalent Omicron-Containing Booster Vaccines against SARS-CoV-2 Omicron Variant among Individuals with and without Prior SARS-CoV-2 Infection

Authors: Kristin Widyasari, Jieun Jang, Taejoon Kang, Sunjoo Kim

Journal: Viruses

PubMed ID: 37632098

Doi: 10.3390/v15081756

Products & Services: Monoclonal Antibody Services

RhoB promotes Salmonella survival by regulating autophagy

Authors: Marco Kirchenwitz, Jessica Halfen, Kristin von Peinen, Silvia Prettin, Jana Kollasser, Susanne Zur Lage, Wulf Blankenfeldt, Cord Brakebusch, Klemens Rottner, Anika Steffen, Theresia E B Stradal

Journal: Eur J Cell Biol

PubMed ID: 37703749

Doi: 10.1016/j.ejcb.2023.151358

Products & Services: Proteins, Expression, Isolation and Analysis

GmNAC039 and GmNAC018 activate the expression of cysteine protease genes to promote soybean nodule senescence

Authors: Haixiang Yu, Aifang Xiao, Jiashan Wu, Haoxing Li, Yan Duan, Qingshan Chen, Hui Zhu, Yangrong Cao

Journal: Plant Cell

PubMed ID: 37177994

Doi: 10.1093/plcell/koad129

Products & Services: Proteins, Expression, Isolation and Analysis

Anti-Melanogenic and Antioxidant Activity of Bifidobacterium longum Strain ZJ1 Extracts, Isolated from a Chinese Centenarian

Authors: Jing Wu , Funa Zhang , Haixia Yu , Shimei Qi , Yu Wu , Weihua Xiao

Journal: Int J Mol Sci .

PubMed ID: 37628988

Doi: 10.3390/ijms241612810

Products & Services: Peptide Synthesis

Design, synthesis and evaluation of ursodeoxycholic acid-cinnamic acid hybrids as potential anti-inflammatory agents by inhibiting Akt/NF-κB and MAPK signaling pathways

Authors: Xiaoxue Li, Yue Hu, Bingxin He, Lingyu Li, Yu Tian, Yingjie Xiao, Hai Shang, Zhongmei Zou

Journal: Eur J Med Chem

PubMed ID: 37678142

Doi: 10.1016/j.ejmech.2023.115785

Products & Services: Proteins, Expression, Isolation and Analysis

Recombinant spike protein vaccines coupled with adjuvants that have different modes of action induce protective immunity against SARS-CoV-2

Authors: Shiho Chiba, Peter J Halfmann, Shun Iida, Yuichiro Hirata, Yuko Sato, Makoto Kuroda, Tammy Armbrust, Samuel Spyra, Tadaki Suzuki, Yoshihiro Kawaoka

Journal: Vaccine

PubMed ID: 37635002

Doi: 10.1016/j.vaccine.2023.08.054

Products & Services: Peptide Synthesis

Homozygous COQ7 mutation: a new cause of potentially treatable distal hereditary motor neuropathy

Authors: Arnaud Jacquier, Julian Theuriet, Fanny Fontaine, Valentine Mosbach, Nicolas Lacoste, Shams Ribault, Valérie Risson, Julien Carras, Laurent Coudert, Thomas Simonet, Philippe Latour, Tanya Stojkovic, Juliette Piard, Anne Cosson, Gaëtan Lesca, Françoise Bouhour, Stéphane Allouche, Hélène Puccio, Antoine Pegat, Laurent Schaeffer

Journal: Brain

PubMed ID: 36454683

Doi: 10.1093/brain/awac453

Products & Services: Gene Synthesis

Optimization and Deoptimization of Codons in SARS-CoV-2 and Related Implications for Vaccine Development

Authors: Xinkai Wu, Ke-Jia Shan, Fuwen Zan, Xiaolu Tang, Zhaohui Qian, Jian Lu

Journal: Adv Sci (Weinh) .

PubMed ID: 37267926

Doi: 10.3390/v16020223

Products & Services: Gene Synthesis

Target delivery of a PD-1-TREM2 scFv by CAR-T cells enhances anti-tumor efficacy in colorectal cancer

Authors: Jian Chen, Tianchuan Zhu, Guanmin Jiang, Qi Zeng, Zhijian Li, Xi Huang

Journal: Mol Cancer

PubMed ID: 37563723

Doi: 10.1186/s12943-023-01830-x

Products & Services: Gene Synthesis

Immunogenicity, safety and reactogenicity of heterologous (third dose) booster vaccination with a full or fractional dose of two different COVID-19 vaccines: A phase 4, single-blind, randomized controlled trial in adults

Authors: Sue Ann Costa Clemens, Natalie Marchevsky, Sarah Kelly, Sally Felle, Ahmed Eldawi, Rupetha Rajasingam, Rawan Mahmud, Teresa Lambe, Merryn Voysey, Isabela Gonzalez, Eveline Pipolo Milan, Maria Cleonice Justino, Sagida Bibi, Parvinder Aley, Ralf Clemens, Andrew J Pollard

Journal: Hum Vaccin Immunother

PubMed ID: 37438960

Doi: 10.1080/21645515.2023.2233400

Products & Services: Proteins, Expression, Isolation and Analysis

Structural basis of histone H2A lysine 119 deubiquitination by Polycomb repressive deubiquitinase BAP1/ASXL1

Authors: Jonathan F Thomas, Marco Igor Valencia-Sánchez, Simone Tamburri, Susan L Gloor, Samantha Rustichelli, Victoria Godínez-López, Pablo De Ioannes, Rachel Lee, Stephen Abini-Agbomson, Kristjan Gretarsson, Jonathan M Burg, Allison R Hickman, Lu Sun, Saarang Gopinath, Hailey F Taylor, Zu-Wen Sun, Ryan J Ezell, Anup Vaidya, Matthew J Meiners, Marcus A Cheek, William J Rice, Vladimir Svetlov, Evgeny Nudler, Chao Lu, Michael-Christopher Keogh, Diego Pasini, Karim-Jean Armache

Journal: Sci Adv

PubMed ID: 37556531

Doi: 10.1126/sciadv.adg9832

Products & Services: Proteins, Expression, Isolation and Analysis

Photoaffinity labelling displacement assay using multiple recombinant protein domains

Authors: David J Fallon, Alex Phillipou, Christopher J Schofield, David House, Nicholas C O Tomkinson, Jacob T Bush

Journal: Biochem J

PubMed ID: 37401534

Doi: 10.1042/BCJ20230129

Products & Services: Proteins, Expression, Isolation and Analysis

SARS-CoV-2 neutralizing antibody bebtelovimab - a systematic scoping review and meta-analysis

Authors: Mabel Nyit Yi Liew, Kok Pim Kua, Shaun Wen Huey Lee, Kon Ken Wong

Journal: Front Immunol

PubMed ID: 37701439

Doi: 10.3389/fimmu.2023.1100263

Products & Services: Molecular Biology Reagents

Preclinical evaluation of manufacturable SARS-CoV-2 spike virus-like particles produced in Chinese Hamster Ovary cells

Authors: Sergio P Alpuche-Lazcano, Matthew Stuible, Bassel Akache, Anh Tran, John Kelly, Sabahudin Hrapovic, Anna Robotham, Arsalan Haqqani, Alexandra Star, Tyler M Renner, Julie Blouin, Jean-Sébastien Maltais, Brian Cass, Kai Cui, Jae-Young Cho, Xinyu Wang, Daria Zoubchenok, Renu Dudani, Diana Duque, Michael J McCluskie, Yves Durocher

Journal: Commun Med (Lond)

PubMed ID: 37612423

Doi: 10.1038/s43856-023-00340-7

Products & Services: Proteins, Expression, Isolation and Analysis

Modified mRNA Formulation and Stability for Cardiac and Skeletal Muscle Delivery

Authors: Magdalena M Żak, Keerat Kaur, Jimeen Yoo, Ann Anu Kurian, Matthew Adjmi, Gayatri Mainkar, Seonghun Yoon, Lior Zangi

Journal: Pharmaceutics

PubMed ID: 37765147

Doi: 10.3390/pharmaceutics15092176

Products & Services: Molecular Biology Reagents

Divergent Pharmacology and Biased Signaling of the Four Melanocortin-4 Receptor Isoforms in Rainbow Trout ()

Authors: Ren-Lei Ji, Ting Liu, Zhi-Shuai Hou, Hai-Shen Wen, Ya-Xiong Tao

Journal: Biomolecules

PubMed ID: 37627313

Doi: 10.3390/biom13081248

Products & Services: Monoclonal Antibody Services

High resolution cryo-EM and crystallographic snapshots of the actinobacterial two-in-one 2-oxoglutarate dehydrogenase

Authors: Lu Yang, Tristan Wagner, Ariel Mechaly, Alexandra Boyko, Eduardo M Bruch, Daniela Megrian, Francesca Gubellini, Pedro M Alzari, Marco Bellinzoni

Journal: Nat Commun

PubMed ID: 37563123

Doi: 10.1038/s41467-023-40253-6

Products & Services: Proteins, Expression, Isolation and Analysis

High-throughput functional dissection of noncoding SNPs with biased allelic enhancer activity for insulin resistance-relevant phenotypes

Authors: Yuan-Yuan Duan, Xiao-Feng Chen, Ren-Jie Zhu, Ying-Ying Jia, Xiao-Ting Huang, Meng Zhang, Ning Yang, Shan-Shan Dong, Mengqi Zeng, Zhihui Feng, Dong-Li Zhu, Hao Wu, Feng Jiang, Wei Shi, Wei-Xin Hu, Xin Ke, Hao Chen, Yunlong Liu, Rui-Hua Jing, Yan Guo, Meng Li, Tie-Lin Yang

Journal: Am J Hum Genet

PubMed ID: 37506691

Doi: 10.1016/j.ajhg.2023.07.002

Products & Services: Gene Synthesis

Identification of sucrose synthase from to favor biocatalytic glycosylation

Authors: Kai Chen, Lei Lin, Ruiqi Ma, Jiajie Ding, Huayi Pan, Yehui Tao, Yan Li, Honghua Jia

Journal: Front Microbiol

PubMed ID: 37649634

Doi: 10.3389/fmicb.2023.1220208

Products & Services: Proteins, Expression, Isolation and Analysis

Conformational investigation of the asymmetric periplasmic domains of LptBFGC using SDSL CW EPR spectroscopy

Authors: Nicholas P Cina, Candice S Klug

Journal: Appl Magn Reson

PubMed ID: 38645307

Doi: 10.1007/s00723-023-01590-3

Products & Services: Gene Synthesis

GPCR activation and GRK2 assembly by a biased intracellular agonist

Authors: Jia Duan , Heng Liu , Fenghui Zhao , Qingning Yuan , Yujie Ji , Xiaoqing Cai , Xinheng He , Xinzhu Li , Junrui Li , Kai Wu , Tianyu Gao , Shengnan Zhu , Shi Lin , Ming-Wei Wang , Xi Cheng , Wanchao Yin , Yi Jiang , Dehua Yang , H Eric Xu

Journal: Nature .

PubMed ID: 37532940

Doi: 10.1038/s41586-023-06395-9

Products & Services: Proteins, Expression, Isolation and Analysis

A non-toxic recombinant Clostridium septicum α toxin induces protective immunity in mice and rabbits

Authors: Jige Du, Irene Meki, Qianlin Li, Ying Liu, Zhen Zhu, Chenfan Pan, Yingju Xia, Lizhi Fu, Liu Yang, Suhui Zhang, Chunsheng Yin, Yufeng Luo, Tuanjie Wang, Bo Liu, Xiaoyun Chen

Journal: Toxicon

PubMed ID: 37543293

Doi: 10.1016/j.toxicon.2023.107234

Products & Services: Proteins, Expression, Isolation and Analysis

Directed evolution of TetR for constructing sensitive and broad-spectrum tetracycline antibiotics whole-cell biosensor

Authors: Shunlan Li, Dongdong Chen, Ziqing Liu, Shipin Tao, Tianyi Zhang, Yiwen Chen, Lingzhi Bao, Jie Ma, Yuee Huang, Shengmin Xu, Lijun Wu, Shaopeng Chen

Journal: J Hazard Mater

PubMed ID: 37633019

Doi: 10.1016/j.jhazmat.2023.132311

Products & Services: Proteins, Expression, Isolation and Analysis

Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors

Authors: Ao Zhang, Tiaofeng Shan, Yan Sun, Zhipeng Chen, Jianjian Hu, Zhichao Hu, Ziheng Ming, Zhitao Zhu, Xue Li, Jun He, Shijia Liu, Ling Jiang, Xiaoou Dong, Yufeng Wu, Yanpeng Wang, Yuqiang Liu, Chao Li, Jianmin Wan

Journal: Plant Biotechnol J

PubMed ID: 37571976

Doi: 10.1111/pbi.14156

Products & Services: Proteins, Expression, Isolation and Analysis

Identification of potential SLA-I-specific T-cell epitopes within the structural proteins of porcine deltacoronavirus

Authors: Yimin Wen, Rui Chen, Junpeng Yang, Enbo Yu, Weizhe Liu, Yijie Liao, Yiping Wen, Rui Wu, Qin Zhao, Senyan Du, Qigui Yan, Xinfeng Han, Sanjie Cao, Xiaobo Huang

Journal: Int J Biol Macromol

PubMed ID: 37579907

Doi: 10.1016/j.ijbiomac.2023.126327

Products & Services: Molecular Biology Reagents

A Systematic Survey of Reversibly Covalent Dipeptidyl Inhibitors of the SARS-CoV-2 Main Protease

Authors: Zhi Zachary Geng, Sandeep Atla, Namir Shaabani, Veerabhadra Vulupala, Kai S Yang, Yugendar R Alugubelli, Kaustav Khatua, Peng-Hsun Chen, Jing Xiao, Lauren R Blankenship, Xinyu R Ma, Erol C Vatansever, Chia-Chuan D Cho, Yuying Ma, Robert Allen, Henry Ji, Shiqing Xu, Wenshe Ray Liu

Journal: J Med Chem

PubMed ID: 37561993

Doi: 10.1021/acs.jmedchem.3c00221

Products & Services: Custom DNA/RNA Oligos

Structural basis for cross-group recognition of an influenza virus hemagglutinin antibody that targets postfusion stabilized epitope

Authors: Keisuke Tonouchi, Yu Adachi, Tateki Suzuki, Daisuke Kuroda, Ayae Nishiyama, Kohei Yumoto, Haruko Takeyama, Tadaki Suzuki, Takao Hashiguchi, Yoshimasa Takahashi

Journal: PLoS Pathog

PubMed ID: 37556494

Doi: 10.1371/journal.ppat.1011554

Products & Services: Peptide Synthesis

Binding and transport of LPS occurs through the coordinated combination of an array of sites across the entire Escherichia coli LPS transport protein LptA

Authors: Kathryn M Schultz, John R Schneider, Matthew A Fischer, Nicholas P Cina, Molly O Riegert, Dara W Frank, Candice S Klug

Journal: Protein Sci

PubMed ID: 37417889

Doi: 10.1002/pro.4724

Products & Services: Gene Synthesis

Optimization of an alum-anchored clinical HIV vaccine candidate

Authors: Kristen A Rodrigues, Christopher A Cottrell, Jon M Steichen, Bettina Groschel, Wuhbet Abraham, Heikyung Suh, Yash Agarwal, Kaiyuan Ni, Jason Y H Chang, Parisa Yousefpour, Mariane B Melo, William R Schief, Darrell J Irvine

Journal: npj Vaccines

PubMed ID: 37573422

Doi: 10.1038/s41541-023-00711-0

Products & Services: Recombinant Proteins

Evaluating the effect of food components on the digestion of dietary nucleic acids in human gastric juice in vitro

Authors: Yanfang Zhang, Jingyi Dong, Jingxian Chen, Xiaoming Pan

Journal: Food Sci Nutr

PubMed ID: 37823154

Doi: 10.1002/fsn3.3599

Products & Services: Custom DNA/RNA Oligos

Toward non-factor therapy in hemophilia: an antithrombin insensitive Gla-domainless factor Xa as tissue factor pathway inhibitor bait

Authors: Marie-Claire Dagher, Atanur Ersayin, Landry Seyve, Mathieu Castellan, Cyril Moreau, Luc Choisnard, Nicole Thielens, Raphaël Marlu, Benoît Polack, Aline Thomas

Journal: Res Pract Thromb Haemost

PubMed ID: 37841511

Doi: 10.1016/j.rpth.2023.102175

Products & Services: Combinatorial DNA Libraries

Immobilization of α-transglucosidase on silica-coated magnetic nanoparticles and its application for production of isomaltooligosaccharide from the potato peel

Authors: Rohit Maurya, Usman Ali, Sunaina Kaul, Raja Bhaiyya, Ravindra Pal Singh, Koushik Mazumder

Journal: Sci Rep

PubMed ID: 37543692

Doi: 10.1038/s41598-023-38266-8

Products & Services: Molecular Biology Reagents

CircMIB2 therapy can effectively treat pathogenic infection by encoding a novel protein

Authors: Weiwei Zheng, Linchao Wang, Shang Geng, Liyuan Yang, Xing Lv, Shiying Xin, Tianjun Xu

Journal: Cell Death Dis

PubMed ID: 37652905

Doi: 10.1038/s41419-023-06105-3

Products & Services: Custom RNAi Services

Exosomal PGAM1 promotes prostate cancer angiogenesis and metastasis by interacting with ACTG1

Authors: Jun-Qi Luo, Tao-Wei Yang, Jun Wu, Hou-Hua Lai, Li-Bin Zou, Wen-Bin Chen, Xu-Min Zhou, Dao-Jun Lv, Sheng-Ren Cen, Zi-Ning Long, Yi-You Mao, Peng-Xiang Zheng, Xiao-Hong Su, Zhi-Yong Xian, Fang-Peng Shu, Xiang-Ming Mao

Journal: Cell Death Dis

PubMed ID: 37542027

Doi: 10.1038/s41419-023-06007-4

Products & Services: Catalog Antibody

The SlWRKY6-SlPROPEP-SlPep module confers tomato resistance to Phytophthora infestans

Authors: Ruirui Yang , Jie Liu , Zhicheng Wang , Lei Zhao , Tingting Xue , Jun Meng , Yushi Luan

Journal: Scientia Horticulturae

Doi: 10.1016/j.scienta.2023.112117

Products & Services: Peptide Synthesis

Mechanosensitive accumulation of non-muscle myosin IIB during mitosis requires its translocation activity

Authors: Chao Wang , Jingjing Ding , Qiaodong Wei , Shoukang Du , Xiaobo Gong , Ting Gang Chew

Journal: iScience .

PubMed ID: 37720093

Doi: 10.1016/j.isci.2023.107773

Products & Services: Proteins, Expression, Isolation and Analysis

Using a synthetic machinery to improve carbon yield with acetylphosphate as the core

Authors: Likun Guo , Min Liu , Yujia Bi , Qingsheng Qi, Mo Xian , Guang Zhao

Journal: Nat Commun .

PubMed ID: 37648707

Doi: 10.1038/s41467-023-41135-7

Products & Services: Codon Optimization

Novel "GaEl Antigenic Patches" Identified by a "Reverse Epitomics" Approach to Design Multipatch Vaccines against NIPAH Infection, a Silent Threat to Global Human Health

Authors: Sukrit Srivastava, Michael Kolbe

Journal: ACS Omega

PubMed ID: 37692250

Doi: 10.1021/acsomega.3c01909

Products & Services: Proteins, Expression, Isolation and Analysis

Peripheral substitution effects on unnatural base pairs: A case of brominated TPT3 to enhance replication fidelity

Authors: Bianbian Huo, Chao Wang, Xiaoqi Hu, Honglei Wang, Gongming Zhu, Anlian Zhu, Lingjun Li

Journal: Bioorg Chem

PubMed ID: 37683537

Doi: 10.1016/j.bioorg.2023.106827

Products & Services: Proteins, Expression, Isolation and Analysis

Context-dependent role of Pho binding sites in Polycomb complex recruitment in Drosophila

Authors: Janet Lesley Brown, Joshua D Price, Maksim Erokhin, Judith A Kassis

Journal: Genetics

PubMed ID: 37216193

Doi: 10.1093/genetics/iyad096

Products & Services: Proteins, Expression, Isolation and Analysis

Time-resolved cryo-EM using a combination of droplet microfluidics with on-demand jetting

Authors: Stefania Torino, Mugdha Dhurandhar, Annelore Stroobants, Raf Claessens, Rouslan G Efremov

Journal: Nat Methods

PubMed ID: 37592181

Doi: 10.1038/s41592-023-01967-z

Products & Services: Proteins, Expression, Isolation and Analysis

Enhancement of Neutralization Responses through Sequential Immunization of Stable Env Trimers Based on Consensus Sequences from Select Time Points by Mimicking Natural Infection

Authors: Mingming Wan, Xiao Yang, Jie Sun, Elena E Giorgi, Xue Ding, Yan Zhou, Yong Zhang, Weiheng Su, Chunlai Jiang, Yaming Shan, Feng Gao

Journal: Int J Mol Sci

PubMed ID: 37628824

Doi: 10.3390/ijms241612642

Products & Services: Gene Synthesis

Rice-produced classical swine fever virus glycoprotein E2 with herringbone-dimer design to enhance immune responses

Authors: Qianru Xu, Fanshu Ma, Daichang Yang, Qingmei Li, Liming Yan, Jiquan Ou, Longxian Zhang, Yunchao Liu, Quan Zhan, Rui Li, Qiang Wei, Hui Hu, Yanan Wang, Xueyang Li, Shenli Zhang, Jifei Yang, Shujun Chai, Yongkun Du, Li Wang, Erqin Zhang, Gaiping Zhang

Journal: Plant Biotechnol J

PubMed ID: 37572354

Doi: 10.1111/pbi.14152

Products & Services: Gene Synthesis

Nanoparticles carrying paclitaxel and anti-miR-221 for breast cancer therapy triggered by ultrasound

Authors: Libo Zhang, Zhen Ren, Jinhui Lü, Xinhai Mo, Jie Lin, Ya Li, Wenjing Ma, Pengfei Liu, Yajing Shen, Qian Zhao, Lu Qian, Xiaoxin Cheng, Zuoren Yu, Bo Zhang

Journal: Cell Death Discov

PubMed ID: 37582832

Doi: 10.1038/s41420-023-01594-9

Products & Services: Custom DNA/RNA Oligos

Precision Genome Engineering in Based on a Broad-Host-Range Vector and CRISPR-Cas9 Technology

Authors: Alex Gussak, Maria Laura Ferrando, Mels Schrama, Peter van Baarlen, Jerry Mark Wells

Journal: ACS Synth Biol

PubMed ID: 37602730

Doi: 10.1021/acssynbio.3c00110

Products & Services: Peptide Synthesis

Structure and design of Langya virus glycoprotein antigens

Authors: Zhaoqian Wang, Matthew McCallum, Lianying Yan, William Sharkey, Young-Jun Park, Ha V Dang, Moushimi Amaya, Ashley Person, Christopher C Broder, David Veesler

Journal: biorxiv

PubMed ID: 37645760

Doi: 10.1101/2023.08.20.554025

Products & Services: ORF cDNA Clones/MolecularCloud

Structural mechanism of synergistic targeting of the nucleosome by PU1 and C/EBPα

Authors: Tengfei Lian, Ruifang Guan, Bing-Rui Zhou, Yawen Bai

Journal: biorxiv

PubMed ID: 37790476

Doi: 10.1101/2023.08.25.554718

Products & Services: Gene Synthesis

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