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Expanding the druggable zinc-finger proteome defines properties of drug-induced degradation

Authors: Miko aj S abicki; Jiho Park; Rados aw P. Nowak; Shourya S. Roy Burman; Jesse Pellman; Charles Zou; Hlib Razumkov; Jeannie Carreiro; Simran Rastogi; Anna Goldstein; Marek M. Nagiec; Katherine A. Donovan; Jianwei Che; Moritz Hunkeler; Qixiang Geng; Chi-Lin Hsu; Megha Lakshminarayan; Chelsea Shu; Rebecca L. Zon; Zuzanna Kozicka; Paul M.C. Park; Jonathan M. Tsai; Hojong Yoon; Lyn H. Jones; Adam S. Sperling; Nathanael S. Gray; Eric S. Fischer; Benjamin L. Ebert

Journal: Molecular Cell

PubMed ID: 40845806

Doi: 10.1016/j.molcel.2025.07.019

Decoding the blueprint of receptor binding by filoviruses through large-scale binding assays and machine learning

Authors: Gorka Lasso; Michael Grodus; Estefania Valencia; Veronica DeJesus; Eliza Liang; Isabel Delwel; Rob H. Bortz; Dmitry Lupyan; Hanna Y. Ehrlich; Adrian A. Castellanos; Andrea Gazzo; Heather L. Wells; Supaporn Wacharapluesadee; Alexandre Tremeau-Bravard; Janine F.R. Seetahal; Tom Hughes; Jimmy Lee; Mei-Ho Lee; Anna R. Sjodin; Marike Geldenhuys; Marinda Mortlock; Isamara Navarrete-Macias; Kirsten Gilardi; Michael R. Willig; Alessandra F.D. Nava; Elisabeth H. Loh; Makda Asrat; Tierra Smiley-Evans; Walter S. Magesa; Sijali Zikankuba; David Wolking; Gerardo Suz n; Rafael Ojeda-Flores; Christine V.F. Carrington; Ariful Islam; Jonathan H. Epstein; Wanda Markotter; Christine K. Johnson; Tracey Goldstein; Barbara A. Han; Jonna A.K. Mazet; Rohit K. Jangra; Kartik Chandran; Simon J. Anthony

Journal: Cell Host & Microbe

PubMed ID: 39818205

Doi: 10.1016/j.chom.2024.12.016

Products & Services: Protein and Antibody Isolation

CRISPR/CasΦ2-mediated gene editing in wheat and rye

Authors: Sanzeng Zhao , Xueying Han , Yachen Zhu , Yuwei Han , Huiyun Liu , Zhen Chen , Huifang Li , Dan Wang , Chaofan Tian , Yuting Yuan , Yajie Guo , Xiaomin Si , Daowen Wang, Xiang Ji

Journal: J Integr Plant Biol

PubMed ID: 38351739

Doi: 10.1111/jipb.13624

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Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction

Authors: Yuzhi Sun 1, Renwang Sheng 2, Zhicheng Cao 1, Chuanquan Liu 2, Jiaxiang Li 1, Po Zhang 1, Yan Du 2, Qingyun Mo 2, Qingqiang Yao 1 3, Jialin Chen 2 3 4, Wei Zhang 2 3 4

Journal: Sci Adv

PubMed ID: 38657071

Doi: 10.1126/sciadv.adm7164

Products & Services: Recombinant Proteins

The Transpeptidase Sortase A Binds Nucleic Acids and Mediates Mammalian Cell Labeling

Authors: Yingzheng Liu, Zhike Lu, Panfeng Wu, Zhaohui Liang, Zhenxing Yu, Ke Ni, Lijia Ma

Journal: Adv Sci (Weinh)

PubMed ID: 38581131

Doi: 10.1002/advs.202305605

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The miR184-3p targets neuron-specific ecdysone inducible protein 78 to promote rice black streaked dwarf virus propagation in its planthopper vector

Authors: Haitao Wang, Yan Dong, Man Wang, Shuo Li, Yijun Zhou, Yinghua Ji

Journal: Pest Manag Sci

PubMed ID: 38676556

Doi: 10.1002/ps.8150

Products & Services: Protein and Antibody Isolation

Biomolecular investigations into BBI reveal an enzymatic mechanism for PUFA isomerisation in bifidobacterium CFA bioconversion strains

Authors: Yongchao Mei, Lulu Chang, Yibin Ji, Bo Yang, Jianxin Zhao, Haiqin Chen, Wei Chen

Journal: Int J Biol Macromol

PubMed ID: 38692553

Doi: 10.1016/j.ijbiomac.2024.131836

Products & Services: Molecular Biology Reagents

Precision miRNA profiling: Electrochemiluminescence powered by CRISPR-Cas13a and hybridization chain reaction

Authors: Jihua Wei, Jiayi Zhang, Wei Wang, Haidong Zhou, Huade Ma, Yuanxun Gong, Qianli Tang, Kai Zhang, Xianjiu Liao

Journal: Anal Chim Acta

PubMed ID: 38719418

Doi: 10.1016/j.aca.2024.342641

Products & Services: DNA Sequencing

IGFBP1a is a nutrient deficient response factor that can inhibit fish reproduction through the hypothalamus-pituitary-ovary axis†

Authors: Wei Li, Hangyu Li, Qiongyao Hu, Linlin Wang, Zhan Yin, Guangfu Hu

Journal: Biol Reprod

PubMed ID: 38374691

Doi: 10.1093/biolre/ioae009

Products & Services: Gene Synthesis

Identification and affinity enhancement of T-cell receptor targeting a KRAS cancer neoantigen

Authors: Mengyu Zhang, Wei Xu, Lingjie Luo, Fenghui Guan, Xiangyao Wang, Pei Zhu, Jianhua Zhang, Xuyu Zhou, Feng Wang, Sheng Ye

Journal: Commun Biol

PubMed ID: 38684865

Doi: 10.1038/s42003-024-06209-2

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Effector enrichment by Candidatus Liberibacter promotes Diaphorina citri feeding via Jasmonic acid pathway suppression

Authors: Hao Liu, Bin Deng, Feng-Hao Chen, Jia-Qi Suo, Ge-Cheng Ouyang, Hui-Lin Lu, Da-Song Chen, Xiang Meng

Journal: Pest Manag Sci

PubMed ID: 38554028

Doi: 10.1002/ps.8107

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Delivering large genes using adeno-associated virus and the CRE-lox DNA recombination system

Authors: Poppy Datta, Kun Do Rhee, Rylee J Staudt, Jacob M Thompson, Ying Hsu, Salma Hassan, Arlene V Drack, Seongjin Seo

Journal: Human Molecular Genetics

PubMed ID: 38645107

Doi: 10.1101/2024.04.10.588864

Products & Services: Molecular Biology Reagents ORF cDNA Clones/MolecularCloud

Artificial Extracellular Vesicles Generated from T Cells Using Different Induction Techniques

Authors: Ekaterina A Zmievskaya, Sabir A Mukhametshin, Irina A Ganeeva, Elvina M Gilyazova, Elvira T Siraeva, Marianna P Kutyreva, Artur A Khannanov, Youyong Yuan, Emil R Bulatov

Journal: Biomedicines

PubMed ID: 38672273

Doi: 10.3390/biomedicines12040919

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Barley yellow dwarf virus-GAV 17K protein disrupts thiamine biosynthesis to facilitate viral infection in plants

Authors: Xiaoyu Han, Xue Yang, Siyu Chen, He Wang, Xiaomin Liu, Daowen Wang, Jin Yang, Linlin Chen, Bingjian Sun, Honglian Li, Yan Shi

Journal: Plant J

PubMed ID: 38635415

Doi: 10.1111/tpj.16772

Products & Services: Protein and Antibody Isolation

Multiplexed gene editing in citrus by using a multi-intron containing Cas9 gene

Authors: Poulami Sarkar, Jorge Santiago Vazquez, Mingxi Zhou, Amit Levy, Zhonglin Mou, Vladimir Orbović

Journal: Transgenic Res

PubMed ID: 38564120

Doi: 10.1007/s11248-024-00380-2

Application of Cas12j for Editing

Authors: Lee Ling Tan, Elena Heng, Chung Yan Leong, Veronica Ng, Lay Kien Yang, Deborah Chwee San Seow, Lokanand Koduru, Yoganathan Kanagasundaram, Siew Bee Ng, Guangrong Peh, Yee Hwee Lim, Fong Tian Wong

Journal: Biomolecules

PubMed ID: 38672502

Doi: 10.3390/biom14040486

Products & Services: Gene Synthesis

Exploring immune evasion of SARS-CoV-2 variants using a pseudotyped system

Authors: Haixiao Duan, Ershuai Zhang, Ge Ren, Yining Cheng, Binfeng Yang, Lirong Liu, Normand Jolicoeur, Han Hu, Yan Xu, Binlei Liu

Journal: Heliyon

PubMed ID: 38699727

Doi: 10.1016/j.heliyon.2024.e29939

Products & Services: Gene Synthesis

Remodeling the Homologous Recombination Mechanism of for High-Level Biosynthesis of Squalene

Authors: Man Xu, Nan Yang, Jiang Pan, Qiang Hua, Chun-Xiu Li, Jian-He Xu

Journal: J Agric Food Chem

PubMed ID: 38635942

Doi: 10.1021/acs.jafc.4c01779

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Chitosan-LeoA-DNA Nanoparticles Promoted the Efficacy of Novel LeoA-DNA Vaccination on Mice Against Helicobacter pylori

Authors: Zahra Ahmadzadeh Chaleshtori, Ali Asghar Rastegari, Hashem Nayeri, Abbas Doosti

Journal: Curr Microbiol

PubMed ID: 38558085

Doi: 10.1007/s00284-024-03642-8

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Safe plant Hsp90 adjuvants elicit an effective immune response against SARS-CoV2-derived RBD antigen

Authors: Victor A Ramos-Duarte, Alejandro Orlowski, Carolina Jaquenod de Giusti, Mariana G Corigliano, Ariel Legarralde, Luisa F Mendoza-Morales, Agustín Atela, Manuel A Sánchez, Valeria A Sander, Sergio O Angel, Marina Clemente

Journal: Vaccine

PubMed ID: 38631949

Doi: 10.1016/j.vaccine.2024.04.036

Products & Services: Custom Vector Construction

Chemoproteomic discovery of a covalent allosteric inhibitor of WRN helicase

Authors: Kristen A Baltgalvis, Kelsey N Lamb, Kent T Symons, Chu-Chiao Wu, Melissa A Hoffman, Aaron N Snead, Xiaodan Song, Thomas Glaza, Shota Kikuchi, Jason C Green, Donald C Rogness, Betty Lam, Maria E Rodriguez-Aguirre, David R Woody, Christie L Eissler, Socorro Rodiles, Seth M Negron, Steffen M Bernard, Eileen Tran, Jonathan Pollock, Ali Tabatabaei, Victor Contreras, Heather N Williams, Martha K Pastuszka, John J Sigler, Piergiorgio Pettazzoni, Markus G Rudolph, Moritz Classen, Doris Brugger, Christopher Claiborne, Jean-Marc Plancher, Isabel Cuartas, Joan Seoane, Laurence E Burgess, Robert T Abraham, David S Weinstein, Gabriel M Simon, Matthew P Patricelli, Todd M Kinsella

Journal: Nature

PubMed ID: 38658751

Doi: 10.1038/s41586-024-07318-y

Products & Services: Recombinant Proteins

CRISPR Screen Identifies the RNA-Binding Protein Eef1a1 as a Key Regulator of Myogenesis

Authors: Weiwei Liu, Wei Wang, Zishuai Wang, Xinhao Fan, Wangchang Li, Yuxin Huang, Xiaogan Yang, Zhonglin Tang

Journal: Int J Mol Sci

PubMed ID: 38732031

Doi: 10.3390/ijms25094816

Products & Services: Custom DNA/RNA Oligos

Roles of a newly lethal cuticular structural protein, AaCPR100A, and its upstream interaction protein, G12-like, in Aedes aegypti

Authors: Jing Chen, Yuchen Wu, Jiukai Chen, Haoran Lu, Gong Cheng, Zhijian Jake Tu, Chenghong Liao, Qian Han

Journal: Int J Biol Macromol

PubMed ID: 38670198

Doi: 10.1016/j.ijbiomac.2024.131704

Products & Services: Protein and Antibody Isolation

High mobility group box 1 mediates inflammatory responses in malignant peritoneal mesothelioma

Authors: Lianfen Wang, Xuesong Wang, Ningning Sun, Weili Liu

Journal: Int Immunopharmacol

PubMed ID: 38613884

Doi: 10.1016/j.intimp.2024.112039

Products & Services: Molecular Biology Reagents

MSC-derived small extracellular vesicles mitigate diabetic retinopathy by stabilizing Nrf2 through miR-143-3p-mediated inhibition of neddylation

Authors: Yueqin Chen, Jun Tong, Cong Liu, Chang He, Jinjin Xiang, Genhong Yao, Huayong Zhang, Zhenggao Xie

Journal: Free Radic Biol Med

PubMed ID: 38604315

Doi: 10.1016/j.freeradbiomed.2024.04.216

Products & Services: Molecular Biology Reagents

Functional screening of the Arabidopsis 2C protein phosphatases family identifies PP2C15 as a negative regulator of plant immunity by targeting BRI1-associated receptor kinase 1

Authors: Zhihong Diao, Rongqian Yang, Yizhu Wang, Junmei Cui, Junhao Li, Qiqi Wu, Yaxin Zhang, Xiaosong Yu, Benqiang Gong, Yan Huang, Guozhi Yu, Huipeng Yao, Jinya Guo, Huaiyu Zhang, Jinbo Shen, Andrea A Gust, Yi Cai

Journal: Mol Plant Pathol

PubMed ID: 38561315

Doi: 10.1111/mpp.13447

Products & Services: Protein and Antibody Isolation

Photo-Responsive Decellularized Small Intestine Submucosa Hydrogels

Authors: Van Thuy Duong, Han Dang Nguyen, Ngoc Ha Luong, Chun-Yi Chang, Chien-Chi Lin

Journal: Advanced Functional Materials

Doi: 10.1002/adfm.202401952

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Detecting m6A at single-molecular resolution via direct RNA sequencing and realistic training data

Authors: Adrian Chan, Isabel S Naarmann-de Vries, Carolin P M Scheitl, Claudia Höbartner,Christoph Dieterich

Journal: Nature Communications

PubMed ID: 38637518

Doi: 10.1038/s41467-024-47661-2

Products & Services: Custom DNA/RNA Oligos

Assembly of SARS-CoV-2 nucleocapsid protein with nucleic acid

Authors: Huaying Zhao, Abdullah M Syed, Mir M Khalid, Ai Nguyen, Alison Ciling, Di Wu, Wai-Ming Yau, Sanjana Srinivasan, Dominic Esposito, Jennifer A Doudna, Grzegorz Piszczek, Melanie Ott, Peter Schuck

Journal: Nucleic Acids Res

PubMed ID: 38587193

Doi: 10.1093/nar/gkae256

Products & Services: Plasmid DNA Preparation

Assessing Weak Anion Binding to Small Peptides

Authors: Corinne L D Gibb, Thien H Tran, Bruce C Gibb

Journal: Journal of the American Chemical Society

PubMed ID: 38592238

Doi: 10.1021/acs.jpcb.4c00657

Products & Services: Peptide Synthesis

Structural Insights into the Unexpected Agonism of Tetracyclic Antidepressants at Serotonin Receptors 5-HT1eR and 5-HT1FR

Authors: Gregory Zilberg, Alexandra K Parpounas, Audrey L Warren, Bianca Fiorillo, Davide Provasi, Marta Filizola, Daniel Wacker

Journal: SCIENCE ADVANCES

PubMed ID: 37986777

Doi: 10.1101/2023.10.05.561100

Products & Services: Protein and Antibody Isolation

Immune microniches shape intestinal T function

Authors: Yisu Gu, Raquel Bartolomé-Casado, Chuan Xu, Alice Bertocchi, Alina Janney, Cornelia Heuberger, Claire F Pearson, Sarah A Teichmann, Emily E Thornton, Fiona Powrie

Journal: Nature

PubMed ID: 38570678

Doi: 10.1038/s41586-024-07251-0

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Antibacterial efficacy and mechanism of Cyprinus carpio chemokine-derived L-10 against multidrug-resistant Escherichia coli infections

Authors: Hao Lu, Chenchen Wang, Wenjia Lu, Xiaodan Li, Gaoyan Wang, Wenqi Dong, Xiangru Wang, Huanchun Chen, Chen Tan

Journal: International journal of antimicrobial agents

PubMed ID: 38325720

Doi: 10.1016/j.ijantimicag.2024.107104

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Development of a multiplex droplet digital PCR method for detection and monitoring of Mycobacterium tuberculosis and drug-resistant tuberculosis

Authors: Yu Jeong Choi, Yoonjung Kim, Hye Jung Park, Dokyun Kim, Hyukmin Lee, Young Ah Kim, Kyung-A Lee

Journal: Ann Clin Microbiol Antimicrob

PubMed ID: 38581051

Doi: 10.1186/s12941-024-00687-2

Products & Services: Molecular Biology Reagents

Generation and characterization of a nanobody against the avian influenza virus H7 subtype

Authors: Xiuqin Huang, Weiye Li, Xuewei Cao, Qi Zhang, Yizhen Lin, Siqi Xu, Xinying Dong, Peiqi Liu, Yutong Liu, Ge He, Kaijian Luo, Saixiang Feng

Journal: Int J Biol Macromol

PubMed ID: 38593899

Doi: 10.1016/j.ijbiomac.2024.131458

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Dietary Hemin Remodels Gut Microbiota and Mediates Tissue Inflammation and Injury in the Small Intestine

Authors: Qian Li, Weixin Ke, Shuai Jiang, Miao Zhang, Kai Shan, Chunbao Li

Journal: Mol Nutr Food Res

PubMed ID: 38676468

Doi: 10.1002/mnfr.202300889

Products & Services: Molecular Biology Reagents

Unveiling orphan receptor-like kinases in plants: novel client discovery using high-confidence library predictions in the Kinase-Client (KiC) assay

Authors: Gabriel Lemes Jorge, Daewon Kim, Chunhui Xu, Sung-Hwan Cho, Lingtao Su, Dong Xu, Laura E Bartley, Gary Stacey, Jay J Thelen

Journal: Front Plant Sci

PubMed ID: 38633461

Doi: 10.3389/fpls.2024.1372361

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Dysfunction of the adhesion G protein-coupled receptor latrophilin 1 (ADGRL1/LPHN1) increases the risk of obesity

Authors: André Nguyen Dietzsch, Hadi Al-Hasani, Joachim Altschmied, Katharina Bottermann, Jana Brendler, Judith Haendeler, Susanne Horn, Isabell Kaczmarek, Antje Körner, Kerstin Krause, Kathrin Landgraf, Diana Le Duc, Laura Lehmann, Stefan Lehr, Stephanie Pick, Albert Ricken, Rene Schnorr, Angela Schulz, Martina Strnadová, Akhil Velluva, Heba Zabri, Torsten Schöneberg, Doreen Thor, Simone Prömel

Journal: Signal Transduct Target Ther

PubMed ID: 38664368

Doi: 10.1038/s41392-024-01810-7

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Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases

Authors: Konstantinos Makryniotis, Efstratios Nikolaivits, George Taxeidis, Jasmina Nikodinovic-Runic, Evangelos Topakas

Journal: Biotechnol J

PubMed ID: 38593303

Doi: 10.1002/biot.202400053

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Development and characterization of a bacterial enzyme cascade reaction system for efficient and stable PET degradation

Authors: Chengyong Wang, Rui Long, Xiran Lin, Wei Liu, Liying Zhu, Ling Jiang

Journal: J Hazard Mater

PubMed ID: 38703683

Doi: 10.1016/j.jhazmat.2024.134480

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Structure-Based Optimization of One Neutralizing Antibody against SARS-CoV-2 Variants Bearing the L452R Mutation

Authors: Yamin Chen, Jialu Zha, Shiqi Xu, Jiang Shao, Xiaoshan Liu, Dianfan Li, Xiaoming Zhang

Journal: Viruses

PubMed ID: 38675908

Doi: 10.3390/v16040566

Products & Services: Molecular Biology Reagents

Alternating Arenavirus Vector Immunization Generates Robust Polyfunctional Genotype Cross-Reactive Hepatitis B Virus-Specific CD8 T-Cell Responses and High Anti-Hepatitis B Surface Antigen Titers

Authors: Sarah Schmidt, Meron Mengistu, Stephane Daffis, Sarah Ahmadi-Erber, Daniela Deutschmann, Tetiana Grigoriev, Ruth Chu, Cleo Leung, Adrian Tomkinson, Mohammad Nizam Uddin, Safiehkhatoon Moshkani, Michael D Robek, Jason Perry, Henning Lauterbach, Klaus Orlinger, Simon P Fletcher, Scott Balsitis

Journal: J Infect Dis

PubMed ID: 37602681

Doi: 10.1093/infdis/jiad340

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Functionalizing Yeast Lipid Droplets as Versatile Biomaterials

Authors: Ankita Suri, Kevin K Y Hu, Tayyaba Younas, Geoff Dumsday, Victoria S Haritos

Journal: Small

PubMed ID: 38566530

Doi: 10.1002/smll.202308463

Products & Services: Protein Electrophoresis and Western

Elucidation of genes enhancing natural product biosynthesis through co-evolution analysis

Authors: Xinran Wang, Ningxin Chen, Pablo Cruz-Morales, Biming Zhong, Yangming Zhang, Jian Wang, Yifan Xiao, Xinnan Fu, Yang Lin, Suneil Acharya, Zhibo Li, Huaxiang Deng, Yuhui Sun, Linquan Bai, Xiaoyu Tang, Jay D Keasling, Xiaozhou Luo

Journal: Nat Metab

PubMed ID: 38609677

Doi: 10.1038/s42255-024-01024-9

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Broadly neutralizing antibodies against emerging delta-coronaviruses

Authors: Megi Rexhepaj, Young-Jun Park, Lisa Perruzza, Daniel Asarnow, Mathew Mccallum, Katja Culap, Christian Saliba, Giada Leoni, Alessio Balmelli, Courtney N Yoshiyama, Miles S Dickinson, Joel Quispe, Jack Taylor Brown, M Alejandra Tortorici, Kaitlin R Sprouse, Ashley L Taylor, Tyler N Starr, Davide Corti, Fabio Benigni, David Veesler

Journal: biorxiv

PubMed ID: 38617231

Doi: 10.1101/2024.03.27.586411

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Pharmacology of PACAP and VIP receptors in the spinal cord highlights the importance of the PAC receptor

Authors: Zoe Tasma, Tayla A Rees, Song Guo, Sheryl Tan, Simon J O'Carroll, Richard L M Faull, Maurice A Curtis, Sarah L Christensen, Debbie L Hay, Christopher S Walker

Journal: Br J Pharmacol

PubMed ID: 38616050

Doi: 10.1111/bph.16376

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Inhibition of USP7 enhances CD8 T cell activity in liver cancer by suppressing PRDM1-mediated FGL1 upregulation

Authors: Lin-Lin Sun, Li-Na Zhao, Jiao Sun, Hong-Feng Yuan, Yu-Fei Wang, Chun-Yu Hou, Pan Lv, Hui-Hui Zhang, Guang Yang, Ning-Ning Zhang, Xiao-Dong Zhang, Wei Lu

Journal: Acta Pharmacol Sin

PubMed ID: 38589688

Doi: 10.1038/s41401-024-01263-2

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Probing altered receptor specificities of antigenically drifting human H3N2 viruses by chemoenzymatic synthesis, NMR, and modeling

Authors: Luca Unione, Augustinus N A Ammerlaan, Gerlof P Bosman, Elif Uslu, Ruonan Liang, Frederik Broszeit, Roosmarijn van der Woude, Yanyan Liu, Shengzhou Ma, Lin Liu, Marcos Gómez-Redondo, Iris A Bermejo, Pablo Valverde, Tammo Diercks, Ana Ardá, Robert P de Vries, Geert-Jan Boons

Journal: Nat Commun

PubMed ID: 38582892

Doi: 10.1038/s41467-024-47344-y

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LINC02605 involved in paediatric Mycoplasma pneumoniae pneumonia complicated with diarrhoea via miR-539-5p/CXCL1 axis

Authors: Yang Zhang, Zeming Wu

Journal: Eur J Clin Invest

PubMed ID: 38662581

Doi: 10.1111/eci.14234

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Structural basis of human Na15 gating mechanisms

Authors: Krishna Chinthalapudi, Rupam Biswas, Ana López-Serrano, Hsiang-Ling Huang, Angelina Ramirez-Navarro, Giovanna Grandinetti, Sarah Heissler, Isabelle Deschênes

Journal: Res Sq

PubMed ID: 38659812

Doi: 10.21203/rs.3.rs-3985999/v1

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