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Origin of the reflectin gene and hierarchical assembly of its protein

American Society for Microbiology. 2016;

Katarzyna Kuczkowska*, Charlotte R. Kleiveland,, Rajna Minic, Lars F. Moen, Lise Øverland, Rannei Tjåland, Harald Carlsen, Tor Lea, Geir Mathiesen* and Vincent G. H. Eijsink

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Gene Synthesis	The ag85besat6 gene fragment encoding the Ag85B-ESAT6 fusion 122 (AgE6) was codon optimized for expression in L. plantarum, synthesized at Genscript 123 (Piscataway, NJ), and cloned into a pUC57 plasmid, yielding pUC-AgE6. The gene fragment 124 encoding AgE6 was amplified from pUC-AgE6 using the AgMluR and AgSalIF primers	Get A Quote