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MASP3 Deficiency in Mice Reduces but Does Not Abrogate Alternative Pathway Complement Activity Due to Intrinsic Profactor D Activity

Authors: Damodar Gullipalli, Takashi Miwa, Madhu Golla, Sayaka Sato, Sree Angampalli, Wen-Chao Song

Journal: J Immunol

PubMed ID: 36988282

Doi: 10.4049/jimmunol.2200932

Products & Services: Gene Synthesis

Development and characterization of nanobodies that specifically target the oncogenic Phosphatase of Regenerating Liver-3 (PRL-3) and impact its interaction with a known binding partner, CNNM3

Authors: Caroline N Smith, Kyle Kihn, Zachary A Williamson, K Martin Chow, Louis B Hersh, Konstantin V Korotkov, Daniel Deredge, Jessica S Blackburn

Journal: PLoS ONE

PubMed ID: 37220097

Doi: 10.1371/journal.pone.0285964

Products & Services: Proteins, Expression, Isolation and Analysis

Translation of non-canonical open reading frames as a cancer cell survival mechanism in childhood medulloblastoma

Authors: Damon A Hofman, Jorge Ruiz-Orera, Ian Yannuzzi, Rakesh Murugesan, Adam Brown, Karl R Clauser, Alexandra L Condurat, Jip T van Dinter, Sem A G Engels, Amy Goodale, Jasper van der Lugt, Tanaz Abid, Li Wang, Kevin N Zhou, Jayne Vogelzang, Keith L Ligon, Timothy N Phoenix, Jennifer A Roth, David E Root, Norbert Hubner, Todd R Golub, Pratiti Bandopadhayay, Sebastiaan van Heesch, John R Prensner

Journal: biorxiv

PubMed ID: 37205492

Doi: 10.1101/2023.05.04.539399

Products & Services: Proteins, Expression, Isolation and Analysis

Site-specific gene knock-in and bacterial phytase gene expression in Cas9 RNP-mediated HDR

Authors: Hassan Zadabbas Shahabadi, Arash Akbarzadeh, Hamideh Ofoghi, Saeid Kadkhodaei

Journal: Front Plant Sci

PubMed ID: 37275253

Doi: 10.3389/fpls.2023.1150436

Products & Services: Proteins, Expression, Isolation and Analysis

Hemophore-like proteins produced by periodontopathogens are recognized by the host immune system and react differentially with IgG antibodies

Authors: Michał Śmiga, Klaudia Siemińska, Soraya C Trindade, Isaac S Gomes-Filho, Ellen K Nobre Dos Santos, Teresa Olczak

Journal: J Oral Microbiol

PubMed ID: 37213663

Doi: 10.1080/20002297.2023.2214455

Products & Services: Peptide Synthesis

Hijacking of Host Plasminogen by () via GAPDH: an Important Virulence Mechanism To Promote Adhesion and Extracellular Matrix Degradation

Authors: Jiying Wang, Shiyang Li, Junhong Chen, Lanxi Gan, Jia Wang, Qiyan Xiong, Zhixin Feng, Quan Li, Zhibang Deng, Xiaomin Yuan, Yanfei Yu

Journal: Microbiol Spectr

PubMed ID: 37199643

Doi: 10.1128/spectrum.00218-23

Products & Services: Molecular Biology Reagents

Ionic displacement of Ca by Pb in calmodulin is affected by arrhythmia-associated mutations

Authors: Valerio Marino, Giuditta Dal Cortivo, Daniele Dell'Orco

Journal: Biochim Biophys Acta Mol Cell Res

PubMed ID: 37201768

Doi: 10.1016/j.bbamcr.2023.119490

Products & Services: Codon Optimization

BcMYB111 Responds to BcCBF2 and Induces Flavonol Biosynthesis to Enhance Tolerance under Cold Stress in Non-Heading Chinese Cabbage

Authors: Xiaoshan Chen , Ying Wu , Zhanghong Yu , Zhanyuan Gao , Qiang Ding , Sayyed Hamad Ahmad Shah , Wenyuan Lin , Ying Li , Xilin Hou

Journal: Int J Mol Sci .

PubMed ID: 37240015

Doi: 10.3390/ijms24108670

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Ovarian juvenile granulosa cell tumors with Ollier's disease in children with IDH1 gene somatic mutation

Authors: Jin Zhang, Renwu Hua, Lishuang Ma, Chao Liu, Yanxia Zhang, Xuemin Lü, Tianren Wang, Naijun Wan

Journal: Front Endocrinol (Lausanne)

PubMed ID: 37324278

Doi: 10.3389/fendo.2023.1093273

Products & Services: Proteins, Expression, Isolation and Analysis

The secretory IgA (sIgA) response in human milk against the SARS-CoV-2 Spike is highly durable and neutralizing for at least 1 year of lactation post-infection

Authors: Xiaoqi Yang, Alisa Fox, Claire DeCarlo, Nicole Pineda, Rebecca L R Powell

Journal: medrxiv

PubMed ID: 37293109

Doi: 10.1101/2023.05.19.23290192

Products & Services: Proteins, Expression, Isolation and Analysis

Characterisation of IL-23 receptor antagonists and disease relevant mutants using fluorescent probes

Authors: Charles S Lay, Albert Isidro-Llobet, Laura E Kilpatrick, Peter D Craggs, Stephen J Hill

Journal: Nat Commun

PubMed ID: 37208328

Doi: 10.1038/s41467-023-38541-2

Products & Services: Proteins, Expression, Isolation and Analysis

Molecular cloning and in silico analysis of chalcone isomerase from Polygonum minus

Authors: Fatin Lyana Azman Shah, Syarul Nataqain Baharum, Hoe-Han Goh, Thean Chor Leow, Ahmad Bazli Ramzi, Siti Nurbaya Oslan, Suriana Sabri

Journal: Mol Biol Rep

PubMed ID: 37148413

Doi: 10.1007/s11033-023-08417-1

Products & Services: Recombinant Proteins

Synthesis and Evaluation of Clinically Translatable Targeted Microbubbles Using a Microfluidic Device for In Vivo Ultrasound Molecular Imaging

Authors: Rakesh Bam, Arutselvan Natarajan, Farbod Tabesh, Ramasamy Paulmurugan, Jeremy J Dahl

Journal: Int J Mol Sci

PubMed ID: 37240396

Doi: 10.3390/ijms24109048

Products & Services: Recombinant Proteins

Soluble monomeric human programmed cell death-ligand 1 inhibits the functions of activated T cells

Authors: Zhaoduan Liang, Wenfang Chen, Yunzhuo Guo, Yuefei Ren, Ye Tian, Wenxuan Cai, Yifeng Bao, Qi Liu, Peng Ding, Yi Li

Journal: Front Immunol

PubMed ID: 37266424

Doi: 10.3389/fimmu.2023.1133883

Products & Services: Stable Cell Lines

Identification of new conserved linear B-cell epitopes in the 3AB and 3C protein of Senecavirus A

Authors: Jian Li, Junfang Yan, Yanni Gao, Xing Liu, Haifeng Sun, Juan Bai, Ping Jiang

Journal: Vet Microbiol

PubMed ID: 37263042

Doi: 10.1016/j.vetmic.2023.109780

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FOSL2 promotes intertumoral infiltration of T cells and increases pathological complete response rates in locally advanced rectal cancer patients

Authors: Kailun Xu, Xiaoyang Yin, Biting Zhou, Xi Zheng, Hao Wang, Jing Chen, Xue Cai, Huanhuan Gao, Xiaoming Xu, Liuhong Wang, Li Shen, Tiannan Guo, Shu Zheng, Baosheng Li, Yingkuan Shao, Jian Wang

Journal: CANCER LETTERS

PubMed ID: 36997107

Doi: 10.1016/j.canlet.2023.216145

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Co-delivery of Cas9 mRNA and guide RNAs edits hepatitis B virus episomal and integration DNA in mouse and tree shrew models

Authors: Junzhu Yi, Xinlin Lei, Fangteng Guo, Qiubing Chen, Xueyong Chen, Kaitao Zhao, Chengliang Zhu, Xiaoming Cheng, Jiangwei Lin, Hao Yin, Yuchen Xia

Journal: Antiviral research

PubMed ID: 37142191

Doi: 10.1016/j.antiviral.2023.105618

Products & Services: CRISPR RNAs/Cas9 Proteins(INACTIVE)

Functionalized Metal–Organic Framework-Modified Hydrogel That Breaks the Vicious Cycle of Inflammation and ROS for Repairing of Diabetic Bone Defects

Authors: An Lao, Jiaqing Wu, Dejian Li, Aili Shen, Yaxin Li, Yu Zhuang, Kaili Lin, Jianyong Wu, Jiaqiang Liu

Journal: Small

PubMed ID: 37183293

Doi: smll.202206919

Products & Services: Proteins, Expression, Isolation and Analysis

Immunogenic SARS-CoV-2 S and N Protein Peptide and Cytokine Combinations as Biomarkers for Early Prediction of Fatal COVID-19

Authors: Ekaterina Martynova , Shaimaa Hamza , Maria Markelova , Ekaterina Garanina , Yuriy Davidyuk , Venera Shakirova , Neha Kaushal , Manoj Baranwal , Robert J Stott-Marshall , Toshana L Foster , Albert Rizvanov , Svetlana Khaiboullina

Journal: Front Immunol .

PubMed ID: 35386707

Doi: 10.3389/fimmu.2022.830715

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In vitro reconstitution of SARS-CoV-2 Nsp1-induced mRNA cleavage reveals the key roles of the N-terminal domain of Nsp1 and the RRM domain of eIF3g

Authors: Irina S. Abaeva, Yani Arhab, Anna Miscicka, Christopher U.T. Hellen, and Tatyana V. Pestova

Journal: bioRxiv .

PubMed ID: 37292671

Doi: 10.1101/gad.350829.123

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Topically Administered NOX4 Inhibitor, GLX7013114, Is Efficacious in Treating the Early Pathological Events of Diabetic Retinopathy

Authors: Stavroula Dionysopoulou, Per Wikstrom, Claudio Bucolo, Giovanni Luca Romano, Vincenzo Micale, Richard Svensson, Dimitris Spyridakos, Niki Mastrodimou, Spiros Georgakis, Panayotis Verginis, Erik Walum, Kyriaki Thermos

Journal: Diabetes

PubMed ID: 36821829

Doi: 10.2337/db22-0515

Products & Services: Proteins, Expression, Isolation and Analysis

The Nucleocapsid Proteins of SARS-CoV-2 and Its Close Relative Bat Coronavirus RaTG13 Are Capable of Inhibiting PKR- and RNase L-Mediated Antiviral Pathways

Authors: Kyle LeBlanc, Jessie Lynch, Christine Layne, Robert Vendramelli, Angela Sloan, Nikesh Tailor, Yvon Deschambault, Fushun Zhang, Darwyn Kobasa, David Safronetz, Yan Xiang, Jingxin Cao

Journal: Microbiol Spectr

PubMed ID: 37154717

Doi: 10.1128/spectrum.00994-23

Products & Services: Proteins, Expression, Isolation and Analysis

KRAS mutation-induced TOPK overexpression contributes to tumour progression in non-small cell lung cancer

Authors: Chang Cai, Shuo Yao, Yanmei Zou, Hui Lu, Xiuqiong Chen, Yali Wang, Kun Zheng, Feng Zhu, Yihua Wang, Hua Xiong, Junfei Zhu

Journal: J Cell Mol Med

PubMed ID: 37226642

Doi: 10.1111/jcmm.17640

Products & Services: Proteins, Expression, Isolation and Analysis

Redundant actions of neuropeptides encoded by the dh-pban gene for larval color pattern formation in the oriental armyworm Mythimnaseparata

Authors: Yu Shirai, Hajime Ono, Takaaki Daimon

Journal: Insect Biochem Mol Biol

PubMed ID: 37146697

Doi: 10.1016/j.ibmb.2023.103955

Products & Services: Peptide Synthesis

Detection of viral RNAs at ambient temperature via reporter proteins produced through the target-splinted ligation of DNA probes

Authors: Elizabeth A Phillips, Adam D Silverman, Aric Joneja, Michael Liu, Carl Brown, Paul Carlson, Christine Coticchia, Kristen Shytle, Alex Larsen, Nadish Goyal, Vincent Cai, Jason Huang, Jennifer E Hickey, Emily Ryan, Joycelynn Acheampong, Pradeep Ramesh, James J Collins, William J Blake

Journal: Nat Biomed Eng

PubMed ID: 37142844

Doi: 10.1038/s41551-023-01028-y

Products & Services: Proteins, Expression, Isolation and Analysis

Modeling cell-mediated immunity in human type 1 diabetes by engineering autoreactive CD8 T cells

Authors: Leeana D Peters, Wen-I Yeh, Juan M Arnoletti, Matthew E Brown, Amanda L Posgai, Clayton E Mathews, Todd M Brusko

Journal: Front Immunol

PubMed ID: 37325626

Doi: 10.3389/fimmu.2023.1142648

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Soluble TREM-like Transcript-1 Acts as a Damage-Associated Molecular Pattern through the TLR4/MD2 Pathway Contributing to Immune Dysregulation during Sepsis

Authors: Chia-Ming Chang, Kuang-Hua Cheng, Tsai-Yin Wei, Meng-Ping Lu, Yi-Chen Chen, Yen-Ta Lu

Journal: J Immunol

PubMed ID: 36961438

Doi: 10.4049/jimmunol.2200222

Products & Services: Proteins, Expression, Isolation and Analysis

Leveraging a Structural Blueprint to Rationally Engineer the Rieske Oxygenase TsaM

Authors: Jiayi Tian, Alejandro Arcadio Garcia, Patrick H Donnan, Jennifer Bridwell-Rabb

Journal: Biochemistry

PubMed ID: 37188334

Doi: 10.1021/acs.biochem.3c00150

Products & Services: Proteins, Expression, Isolation and Analysis

Antigen-Induced IL-1RA Production Discriminates Active and Latent Tuberculosis Infection

Authors: Cesar Sanchez, Luis Jaramillo-Valverde, Silvia Capristano, Gilmer Solis, Alonso Soto, Julio Valdivia-Silva, Julio A Poterico, Heinner Guio

Journal: Microorganisms

PubMed ID: 37374887

Doi: 10.3390/microorganisms11061385

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Antibody blockade of Jagged1 attenuates choroidal neovascularization

Authors: Torleif Tollefsrud Gjølberg, Jonas Aakre Wik, Hanna Johannessen, Stig Krüger, Nicola Bassi, Panagiotis F Christopoulos, Malin Bern, Stian Foss, Goran Petrovski, Morten C Moe, Guttorm Haraldsen, Johanna Hol Fosse, Bjørn Steen Skålhegg, Jan Terje Andersen, Eirik Sundlisæter

Journal: Nat Commun

PubMed ID: 37253747

Doi: 10.1038/s41467-023-38563-w

Products & Services: Proteins, Expression, Isolation and Analysis

Small heterodimer partner interacting leucine zipper protein (SMILE) ameliorates autoimmune arthritis via AMPK signaling pathway and the regulation of B cell activation

Authors: JooYeon Jhun, Jeonghyeon Moon, Ji Ye Kwon, Keun-Hyung Cho, Seang Yoon Lee, Hyun Sik Na, Mi-La Cho, Jun-Ki Min

Journal: Cell Commun Signal

PubMed ID: 37143079

Doi: 10.1186/s12964-023-01054-y

Products & Services: Codon Optimization

Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrest

Authors: Bipul R Acharya, Jennifer S Fang, Erin D Jeffery, Nicholas W Chavkin, Gael Genet, Hema Vasavada, Elizabeth A Nelson, Gloria M Sheynkman, Martin J Humphries, Karen K Hirschi

Journal: Life Sci Alliance

PubMed ID: 37197981

Doi: 10.26508/lsa.202201685

Products & Services: Molecular Biology Reagents

Conserved cardiolipin-mitochondrial ADP/ATP carrier interactions assume distinct structural and functional roles that are clinically relevant

Authors: Nanami Senoo, Dinesh K Chinthapalli, Matthew G Baile, Vinaya K Golla, Bodhisattwa Saha, Oluwaseun B Ogunbona, James A Saba, Teona Munteanu, Yllka Valdez, Kevin Whited, Dror Chorev, Nathan N Alder, Eric R May, Carol V Robinson, Steven M Claypool

Journal: biorxiv

PubMed ID: 37205478

Doi: 10.1101/2023.05.05.539595

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GSK3 phosphorylates and regulates the Green Revolution protein Rht-B1b to reduce plant height in wheat

Authors: Huixue Dong, Danping Li, Ruizhen Yang, Lichao Zhang, Yunwei Zhang, Xu Liu, Xiuying Kong, Jiaqiang Sun

Journal: Plant Cell

PubMed ID: 36945740

Doi: 10.1093/plcell/koad090

Products & Services: Recombinant Proteins

A highly efficient transgene knock-in technology in clinically relevant cell types

Authors: Alexander G Allen, Samia Q Khan, Carrie M Margulies, Ramya Viswanathan, Swarali Lele, Laura Blaha, Sean N Scott, Kaitlyn M Izzo, Alexandra Gerew, Rithu Pattali, Nadire R Cochran, Carl S Holland, Amy H Zhao, Stephen E Sherman, Michael C Jaskolka, Meng Wu, Aaron C Wilson, Xiaoqi Sun, Dawn M Ciulla, Deric Zhang, Jacqueline D Nelson, Peisheng Zhang, Patrizia Mazzucato, Yan Huang, Georgia Giannoukos, Eugenio Marco, Michael Nehil, John A Follit, Kai-Hsin Chang, Mark S Shearman, Christopher J Wilson, John A Zuris

Journal: Nat Biotechnol

PubMed ID: 37127662

Doi: 10.1038/s41587-023-01779-8

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Deep learning models to predict the editing efficiencies and outcomes of diverse base editors

Authors: Nahye Kim, Sungchul Choi, Sungjae Kim, Myungjae Song, Jung Hwa Seo, Seonwoo Min, Jinman Park, Sung-Rae Cho, Hyongbum Henry Kim

Journal: Nat Biotechnol

PubMed ID: 37188916

Doi: 10.1038/s41587-023-01792-x

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Structure of a 10-23 Deoxyribozyme Exhibiting a Homodimer Conformation

Authors: Evan Cramer, Sarah Starcovic, Rebekah Avey, Ali Kaya, Aaron Robart

Journal: Res Sq

PubMed ID: 37398199

Doi: 10.21203/rs.3.rs-2252941/v1

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Engineering Rapalog-Inducible Genetic Switches Based on Split-T7 Polymerase to Regulate Oncolytic Virus-Driven Production of Tumour-Localized IL-12 for Anti-Cancer Immunotherapy

Authors: Nikolas T Martin, Mathieu J F Crupi, Zaid Taha, Joanna Poutou, Jack T Whelan, Sydney Vallati, Julia Petryk, Ricardo Marius, Bradley Austin, Taha Azad, Mason Boulanger, Tamara Burgess, Ilson Sanders, Camille Victoor, Bryan C Dickinson, Jean-Simon Diallo, Carolina S Ilkow, John C Bell

Journal: Pharmaceuticals (Basel)

PubMed ID: 37242495

Doi: 10.3390/ph16050709

Products & Services: Recombinant Proteins

Evaluation of humoral and cellular immune responses induced by a cocktail of recombinant African swine fever virus antigens fused with OprI in domestic pigs

Authors: Guanglei Zhang, Wei Liu, Sicheng Yang, Shuai Song, Yunyun Ma, Guangqing Zhou, Xiaxia Liang, Chun Miao, Junhui Li, Yanhong Liu, Junjun Shao, Huiyun Chang

Journal: Virol J

PubMed ID: 37237390

Doi: 10.1186/s12985-023-02070-7

Products & Services: DNA Sequencing

Design and development of microformulations for rapid release of small molecules and oligonucleotides

Authors: T Brown, M Stanton, F Cros, S Cho, A Kiselyov

Journal: Eur J Pharm Sci

PubMed ID: 37220816

Doi: 10.1016/j.ejps.2023.106472

Products & Services: Proteins, Expression, Isolation and Analysis

Enhanced stability of the SARS CoV-2 spike glycoprotein following modification of an alanine cavity in the protein core

Authors: Pantelis Poumbourios, Christine Langer, Irene Boo, Tasnim Zakir, Rob J Center, Anouschka Akerman, Vanessa Milogiannakis, Anupriya Aggarwal, Bronte A Johnstone, Jungmin Ha, Fasséli Coulibaly, Stuart G Turville, Heidi E Drummer

Journal: PLoS Pathog

PubMed ID: 37200378

Doi: 10.1371/journal.ppat.1010981

Products & Services: Proteins, Expression, Isolation and Analysis

Rescue of secretion of a rare-disease associated mis-folded mutant glycoprotein in knock-out mammalian cells

Authors: Gábor Tax, Kevin P Guay, Tatiana Soldà, Charlie J Hitchman, Johan C Hill, Snežana Vasiljević, Andrea Lia, Carlos P Modenutti, Kees R Straatman, Angelo Santino, Maurizio Molinari, Nicole Zitzmann, Daniel N Hebert, Pietro Roversi, Marco Trerotola

Journal: biorxiv

PubMed ID: 37398215

Doi: 10.1101/2023.05.30.542711

Products & Services: Proteins, Expression, Isolation and Analysis

The E3 Ubiquitin Ligase SCF Cyclin F Promotes Sequestosome-1/p62 Insolubility and Foci Formation and is Dysregulated in ALS and FTD Pathogenesis

Authors: Jennilee M Davidson, Sharlynn S L Wu, Stephanie L Rayner, Flora Cheng, Kimberley Duncan, Carlo Russo, Michelle Newbery, Kunjie Ding, Natalie M Scherer, Rachelle Balez, Alberto García-Redondo, Alberto Rábano, Livia Rosa-Fernandes, Lezanne Ooi, Kelly L Williams, Marco Morsch, Ian P Blair, Antonio Di Ieva, Shu Yang, Roger S Chung, Albert Lee

Journal: Mol Neurobiol

PubMed ID: 37243816

Doi: 10.1007/s12035-023-03355-2

Products & Services: Proteins, Expression, Isolation and Analysis

saRNA vaccine expressing membrane-anchored RBD elicits broad and durable immunity against SARS-CoV-2 variants of concern

Authors: Mai Komori, Takuto Nogimori, Amber L Morey, Takashi Sekida, Keiko Ishimoto, Matthew R Hassett, Yuji Masuta, Hirotaka Ode, Tomokazu Tamura, Rigel Suzuki, Jeff Alexander, Yasutoshi Kido, Kenta Matsuda, Takasuke Fukuhara, Yasumasa Iwatani, Takuya Yamamoto, Jonathan F Smith, Wataru Akahata

Journal: Nat Commun

PubMed ID: 37208330

Doi: 10.1038/s41467-023-38457-x

Products & Services: Proteins, Expression, Isolation and Analysis

Fusion of the molecular adjuvant C3d to cleavage-independent native-like HIV-1 Env trimers improves the elicited antibody response

Authors: Shridhar Bale, Lifei Yang, Mehrdad Alirezaei, Richard Wilson, Takayuki Ota, Esmeralda D Doyle, Christopher A Cottrell, Javier Guenaga, Karen Tran, Wenjuan Li, Leonidas Stamatatos, David Nemazee, Andrew B Ward, Richard T Wyatt

Journal: Front Immunol

PubMed ID: 37283743

Doi: 10.3389/fimmu.2023.1180959

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CFTR and dystrophin encoding plasmids carrying both luciferase reporter gene, nuclear import specific sequences and triple helix sites

Authors: Delphine Maze, Caroline Girardin, Nathalie Benz, Tristan Montier, Chantal Pichon, Patrick Midoux

Journal: Plasmid

PubMed ID: 37207938

Doi: 10.1016/j.plasmid.2023.102686

Products & Services: Proteins, Expression, Isolation and Analysis

Inflammation Modifies miR-21 Expression Within Neuronal Extracellular Vesicles to Regulate Remyelination Following Spinal Cord Injury

Authors: Tianyu Han, Peiwen Song, Zuomeng Wu, Yunlei Liu, Wang Ying, Cailiang Shen

Journal: Stem Cell Rev Rep

PubMed ID: 37256514

Doi: 10.1007/s12015-023-10560-y

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Predictive data-driven modeling of C-terminal tyrosine function in the EGFR signaling network

Authors: Jacqueline S Gerritsen, Joseph S Faraguna, Rudy Bonavia, Frank B Furnari, Forest M White

Journal: Life Sci Alliance

PubMed ID: 37169593

Doi: 10.26508/lsa.202201466

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Deep sampling of gRNA in the human genome and deep-learning-informed prediction of gRNA activities

Authors: Heng Zhang, Jianfeng Yan, Zhike Lu, Yangfan Zhou, Qingfeng Zhang, Tingting Cui, Yini Li, Hui Chen, Lijia Ma

Journal: Cell Discov

PubMed ID: 37193681

Doi: 10.1038/s41421-023-00549-9

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Simultaneous enhancement of multiple functional properties using evolution-informed protein design

Authors: Benjamin Fram, Ian Truebridge, Yang Su, Adam J Riesselman, John B Ingraham, Alessandro Passera, Eve Napier, Nicole N Thadani, Samuel Lim, Kristen Roberts, Gurleen Kaur, Michael Stiffler, Debora S Marks, Christopher D Bahl, Amir R Khan, Chris Sander, Nicholas P Gauthier

Journal: biorxiv

PubMed ID: 37214973

Doi: 10.1101/2023.05.09.539914

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Preparation of magnetic polyacrylamide hydrogel with chitosan for immobilization of glutamate decarboxylase to produce γ-aminobutyric acid

Authors: Jianjun Wang, Luyao Wang, Chengli Yang, Yihui Zhu, Ziqian Chen, Guanya He, Kaishun Hu, Kaixuan Liu, Beibei Fang, Dali Li, Ruofu Shi

Journal: Prep Biochem Biotechnol

PubMed ID: 37184437

Doi: 10.1080/10826068.2023.2209884

Products & Services: Proteins, Expression, Isolation and Analysis

Reticular adhesions are assembled at flat clathrin lattices and opposed by active integrin α5β1

Authors: Laura Hakanpää, Amr Abouelezz, An-Sofie Lenaerts, Seyda Culfa, Michael Algie, Jenny Bärlund, Pekka Katajisto, Harvey McMahon, Leonardo Almeida-Souza

Journal: J Cell Biol

PubMed ID: 37233325

Doi: 10.1083/jcb.202303107

Products & Services: Proteins, Expression, Isolation and Analysis

Arabidopsis calmodulin-like proteins CML13 and CML14 interact with proteins that have IQ domains

Authors: Howard J Teresinski, Bryan Hau, Kyle Symonds, Ryan Kilburn, Kim A Munro, Nathan M Doner, Robert Mullen, Vivian H Li, Wayne A Snedden

Journal: Plant Cell Environ

PubMed ID: 37222394

Doi: 10.1111/pce.14616

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Fortuitous somatic mutations during antibody evolution endow broad neutralization against SARS-CoV-2 Omicron variants

Authors: Jianbo Wu, Zhenguo Chen, Yidan Gao, Zegen Wang, Jiarong Wang, Bing-Yu Chiang, Yunjiao Zhou, Yuru Han, Wuqiang Zhan, Minxiang Xie, Weiyu Jiang, Xiang Zhang, Aihua Hao, Anqi Xia, Jiaying He, Song Xue, Christian T Mayer, Fan Wu, Bin Wang, Lunan Zhang, Lei Sun, Qiao Wang

Journal: Cell Rep

PubMed ID: 37178120

Doi: 10.1016/j.celrep.2023.112503

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Design and application of artificial rare L-lysine codons in Corynebacterium glutamicum

Authors: Cuiping Yang , Zehao Peng , Lu Yang , Bowen Du , Chuanzhuang Guo , Songsen Sui , Jianbin Wang , Junlin Li , Junqing Wang , Nan Li

Journal: Front Bioeng Biotechnol

PubMed ID: 7324439

Doi: 10.3389/fbioe.2023.1194511

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JNK3 Overexpression in the Entorhinal Cortex Impacts on the Hippocampus and Induces Cognitive Deficiencies and Tau Misfolding

Authors: Carlos G Ardanaz, Amaia Ezkurdia, Arantza Bejarano, Beatriz Echarte, Cristian Smerdou, Eva Martisova, Iván Martínez-Valbuena, María-Rosario Luquin, María J Ramírez, Maite Solas

Journal: ACS Chem Neurosci

PubMed ID: 37236204

Doi: 10.1021/acschemneuro.3c00092

Products & Services: Proteins, Expression, Isolation and Analysis

CLASPs stabilize the pre-catastrophe intermediate state between microtubule growth and shrinkage

Authors: Elizabeth J Lawrence, Saptarshi Chatterjee, Marija Zanic

Journal: J Cell Biol

PubMed ID: 37184584

Doi: 10.1083/jcb.202107027

Products & Services: Proteins, Expression, Isolation and Analysis

Alpha-synuclein-associated changes in PINK1-PRKN-mediated mitophagy are disease context dependent

Authors: Xu Hou, Taylor Hsuan-Yu Chen, Shunsuke Koga, Jenny M Bredenberg, Ayman H Faroqi, Marion Delenclos, Guojun Bu, Zbigniew K Wszolek, Jonathan A Carr, Owen A Ross, Pamela J McLean, Melissa E Murray, Dennis W Dickson, Fabienne C Fiesel, Wolfdieter Springer

Journal: Brain Pathol

PubMed ID: 37259617

Doi: 10.1111/bpa.13175

Products & Services: Proteins, Expression, Isolation and Analysis

Single point mutations at the S129 residue of α-synuclein and their effect on structure, aggregation, and neurotoxicity

Authors: Esha Pandit, Lopamudra Das, Anoy Kumar Das, Sandip Dolui, Saumen Saha, Uttam Pal, Animesh Mondal, Joydeep Chowdhury, Subhas C Biswas, Nakul C Maiti

Journal: Front Chem

PubMed ID: 37304685

Doi: 10.3389/fchem.2023.1145877

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Massively parallel evaluation and computational prediction of the activities and specificities of 17 small Cas9s

Authors: Sang-Yeon Seo, Seonwoo Min, Sungtae Lee, Jung Hwa Seo, Jinman Park, Hui Kwon Kim, Myungjae Song, Dawoon Baek, Sung-Rae Cho, Hyongbum Henry Kim

Journal: Nat Methods

PubMed ID: 37188955

Doi: 10.1038/s41592-023-01875-2

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The effects of free Cys residues on the structure, activity, and tetrameric stability of mammalian uricase

Authors: Yong Guo, Jingjing Huo, Runchao Bai, Jingyuan Zhang, Jipeng Yao, Kaijie Ma, Zengtao Zhang, Haigang Li, Chun Zhang

Journal: Appl Microbiol Biotechnol

PubMed ID: 37256327

Doi: 10.1007/s00253-023-12597-y

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An efficient transformation method for genome editing of elite bread wheat cultivars

Authors: Akshaya K Biswal, L Ruben B Hernandez, Ana I R Castillo, Juan M Debernardi, Kanwarpal S Dhugga

Journal: Front Plant Sci

PubMed ID: 37275249

Doi: 10.3389/fpls.2023.1135047

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L-Lactate treatment by photosynthetic cyanobacteria expressing heterogeneous L-lactate dehydrogenase

Authors: Yuichi Kato, Kosuke Inabe, Yuji Haraguchi, Tatsuya Shimizu, Akihiko Kondo, Tomohisa Hasunuma

Journal: Sci Rep

PubMed ID: 37142758

Doi: 10.1038/s41598-023-34289-3

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Vesicular Stomatitis Virus (VSV) G Glycoprotein Can Be Modified to Create a Her2/Neu-Targeted VSV That Eliminates Large Implanted Mammary Tumors

Authors: Yanhua Gao, Ira Bergman

Journal: J Virol

PubMed ID: 37199666

Doi: 10.1128/jvi.00372-23

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Computational engineering of previously crystallized pyruvate formate-lyase activating enzyme reveals insights into SAM binding and reductive cleavage

Authors: James D Moody, Sarah Hill, Maike N Lundahl, Aubrianna J Saxton, Amanda Galambas, William E Broderick, C Martin Lawrence, Joan B Broderick

Journal: J Biol Chem

PubMed ID: 37156396

Doi: 10.1016/j.jbc.2023.104791

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Identification of a Transmembrane Protein Involved in Shear Stress Signaling and Hepatocarcinogenesis After a Sustained Virological Response to Hepatitis C Virus

Authors: Masashi Nishikawa, Hikari Okada, Kazunori Kawaguchi, Tetsuro Shimakami, Kouki Nio, Kuniaki Arai, Tatsuya Yamashita, Motoko Sasaki, Shuichi Kaneko, Taro Yamashita, Masao Honda

Journal: Cell Mol Gastroenterol Hepatol

PubMed ID: 37146715

Doi: 10.1016/j.jcmgh.2023.04.006

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Modular Hydrogel Vaccine for Programmable and Coordinate Elicitation of Cancer Immunotherapy

Authors: Panpan Ji, Wenqi Sun, Siyan Zhang, Yuqi Xing, Chen Wang, Mengying Wei, Qiuyun Li, Gang Ji, Guodong Yang

Journal: Adv Sci (Weinh)

PubMed ID: 37222047

Doi: 10.1002/advs.202301789

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The free-living flagellate Paratrimastix pyriformis uses a distinct mitochondrial carrier to balance adenine nucleotide pools

Authors: Justyna Zítek, Martin S King, Priscila Peña-Diaz, Eva Pyrihová, Alannah C King, Edmund R S Kunji, Vladimír Hampl

Journal: Arch Biochem Biophys

PubMed ID: 37192692

Doi: 10.1016/j.abb.2023.109638

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Multiplexed Gene Engineering Based on dCas9 and gRNA-tRNA Array Encoded on Single Transcript

Authors: Chaoqian Jiang, Lishuang Geng, Jinpeng Wang, Yingjuan Liang, Xiaochen Guo, Chang Liu, Yunjing Zhao, Junxue Jin, Zhonghua Liu, Yanshuang Mu

Journal: Int J Mol Sci

PubMed ID: 37239880

Doi: 10.3390/ijms24108535

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Functional characterization of novel or yet uncharacterized ATP7B missense variants detected in patients with clinical Wilson's disease

Authors: Amelie Stalke, Annika Behrendt, Finja Hennig, Holger Gohlke, Nicole Buhl, Thea Reinkens, Ulrich Baumann, Brigitte Schlegelberger, Thomas Illig, Eva-Doreen Pfister, Britta Skawran

Journal: Clin Genet

PubMed ID: 37157876

Doi: 10.1111/cge.14352

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Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases

Authors: Guangxue Liu, Jimin Li, Boxue He, Jiaqi Yan, Jingyu Zhao, Xuejie Wang, Xiaocong Zhao, Jingyan Xu, Yeyao Wu, Simin Zhang, Xiaoli Gan, Chun Zhou, Xiangpan Li, Xinghua Zhang, Xuefeng Chen

Journal: Nat Commun

PubMed ID: 37230987

Doi: 10.1038/s41467-023-38617-z

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Antimicrobial activity of the Flo peptide produced in Scenedesmus acutus and Nannochloropsis oculata

Authors: Saraí Damaris Dávalos-Guzmán, Fidel Martinez-Gutierrez, Luzmila Martínez-González, Jesús Josafath Quezada-Rivera, Ana Cecilia Lorenzo-Leal, Horacio Bach, José Francisco Morales-Domínguez, Ruth Elena Soria-Guerra

Journal: World J Microbiol Biotechnol

PubMed ID: 37249711

Doi: 10.1007/s11274-023-03664-7

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Circ_0069094 regulates malignant phenotype and paclitaxel resistance in breast cancer cells via targeting the miR-136-5p/YWHAZ axis

Authors: Zhihua Kong, Qi Han, Bisheng Zhu, Long Wan, Enrong Feng

Journal: Thorac Cancer

PubMed ID: 37192740

Doi: 10.1111/1759-7714.14928

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Resin and loading condition screening for effective and robust byproducts removal by anion exchange chromatography: A case study

Authors: Wei Chen, Peter K Wang, Chien-Chun Liao, Yifeng Li, Yan Wan

Journal: Protein Expr Purif

PubMed ID: 37209930

Doi: 10.1016/j.pep.2023.106297

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Efficient precise integration of large DNA sequences with 3′-overhang dsDNA donors using CRISPR/Cas9

Authors: Wenjie Han, Zhigang Li, Yijun Guo, Kaining He, Wenqing Li, Caoling Xu, Lishuang Ge, Miao He, Xue Yin, Junxiang Zhou, Chengxu Li, Dongbao Yao, Jianqiang Bao, Haojun Liang

Journal: Proceedings of the national academy of sciences of the USA

PubMed ID: 37216515

Doi: 10.1073/pnas.2221127120

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Full-length versus truncated α-factor secretory signal sequences for expression of recombinant human insulin precursor in yeast Pichia pastoris: a comparison

Authors: Nuruliawaty Utami, Dini Nurdiani, Hariyatun Hariyatun, Eko Wahyu Putro, Fadillah Putri Patria, Wien Kusharyoto

Journal: J Genet Eng Biotechnol

PubMed ID: 37212962

Doi: 10.1186/s43141-023-00521-w

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A glycan receptor kinase facilitates intracellular accommodation of arbuscular mycorrhiza and symbiotic rhizobia in the legume Lotus japonicus

Authors: Simon Kelly, Simon B Hansen, Henriette Rübsam, Pia Saake, Emil B Pedersen, Kira Gysel, Eva Madland, Shunliang Wu, Stephan Wawra, Dugald Reid, John T Sullivan, Zuzana Blahovska, Maria Vinther, Artur Muszynski, Parastoo Azadi, Mikkel B Thygesen, Finn L Aachmann, Clive W Ronson, Alga Zuccaro, Kasper R Andersen, Simona Radutoiu, Jens Stougaard

Journal: PLoS Biol

PubMed ID: 37200394

Doi: 10.1371/journal.pbio.3002127

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The adhesion GPCRs CELSR1-3 and LPHN3 engage G proteins via distinct activation mechanisms

Authors: Duy Lan Huong Bui, Andrew Roach, Jingxian Li, Sumit J Bandekar, Elizabeth Orput, Ritika Raghavan, Demet Araç, Richard C Sando

Journal: Cell Rep

PubMed ID: 37224017

Doi: 10.1016/j.celrep.2023.112552

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Structural basis of epitope selectivity and potent protection from malaria by PfCSP antibody L9

Authors: Gregory M Martin, Monica L Fernández-Quintero, Wen-Hsin Lee, Tossapol Pholcharee, Lisa Eshun-Wilson, Klaus R Liedl, Marie Pancera, Robert A Seder, Ian A Wilson, Andrew B Ward

Journal: Nat Commun

PubMed ID: 37198165

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A rapidly evolving single copy histone H1 variant is associated with male fertility in a parasitoid wasp

Authors: Bo Yuan, Yi Yang, Zhichao Yan, Chun He, Yu H Sun, Fei Wang, Beibei Wang, Jiamin Shi, Shan Xiao, Fang Wang, Qi Fang, Fei Li, Xinhai Ye, Gongyin Ye

Journal: Front Cell Dev Biol

PubMed ID: 37325562

Doi: 10.3389/fcell.2023.1166517

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Antagonizing the irreversible thrombomodulin-initiated proteolytic signaling alleviates age-related liver fibrosis via senescent cell killing

Authors: Christopher C Pan, Raquel Maeso-Díaz, Tylor R Lewis, Kun Xiang, Lianmei Tan, Yaosi Liang, Liuyang Wang, Fengrui Yang, Tao Yin, Calvin Wang, Kuo Du, De Huang, Seh Hoon Oh, Ergang Wang, Bryan Jian Wei Lim, Mengyang Chong, Peter B Alexander, Xuebiao Yao, Vadim Y Arshavsky, Qi-Jing Li, Anna Mae Diehl, Xiao-Fan Wang

Journal: Cell Res

PubMed ID: 37169907

Doi: 10.1038/s41422-023-00820-4

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Improved biosynthesis of heme in Bacillus subtilis through metabolic engineering assisted fed-batch fermentation

Authors: Shaomei Yang, Anlong Wang, Jiachang Li, Yunhang Shao, Fengjie Sun, Shucheng Li, Kai Cao, Hongliang Liu, Peng Xiong, Zhengquan Gao

Journal: Microb Cell Fact

PubMed ID: 37198628

Doi: 10.1186/s12934-023-02077-3

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Transcriptome Analysis and Identification of Sesquiterpene Synthases in Liverwort Jungermannia exsertifolia

Authors: Xiaoguang Yan, Yukun Li, Weiguo Li, Dongmei Liang, Shengxin Nie, Ruiqi Chen, Jianjun Qiao, Mingzhang Wen, Qinggele Caiyin

Journal: Bioengineering (Basel) .

PubMed ID: 37237639

Doi: 10.3390/bioengineering10050569

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Early microglial response, myelin deterioration and lethality in mice deficient for very long chain ceramide synthesis in oligodendrocytes

Authors: Jonathan D Teo , Oana C Marian , Alanna G Spiteri , Madeline Nicholson , Huitong Song , Jasmine X Y Khor , Holly P McEwen , Anjie Ge , Monokesh K Sen , Laura Piccio , Jessica L Fletcher , Nicholas J C King , Simon S Murray , Jens C Brüning , Anthony S Don

Journal: Glia .

PubMed ID: 36583573

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A molecular study on recombinant pullulanase type I from Metabacillus indicus.

Authors: Zahraa Z Al-Mamoori , Amira M Embaby , Ahmed Hussein , Hoda E Mahmoud

Journal: AMB Express

PubMed ID: 37119334

Doi: 10.1186/s13568-023-01545-8.

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Adjuvant-Free COVID-19 Vaccine with Glycoprotein Antigen Oxidized by Periodate Rapidly Elicits Potent Immune Responses

Authors: Ru-Yan Zhang, Shi-Hao Zhou, Ran-Ran Feng, Yu Wen, Dong Ding, Zhi-Ming Zhang, Hua-Wei Wei, Jun Guo

Journal: ACS Chemical Biology

PubMed ID: 37009726

Doi: 10.1021/acschembio.3c00050

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LvCD14L Acts as a Novel Pattern Recognition Receptor and a Regulator of the Toll Signaling Pathway in Shrimp

Authors: Xinjia Lv, Shihao Li 1,2,3, Yang Yu, Songjun Jin, Xiaojun Zhang, Fuhua Li

Journal: International Journal of Molecular Sciences

Doi: 10.3390/ijms24097770

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Cryo-EM structures and binding of mouse and human ACE2 to SARS-CoV-2 variants of concern indicate that mutations enabling immune escape could expand host range

Authors: Dongchun Ni, Priscilla Turelli, Bertrand Beckert, Sergey Nazarov, Emiko Uchikawa, Alexander Myasnikov, Florence Pojer, Didier Trono, Henning Stahlberg, Kelvin Lau

Journal: PLoS pathogens

PubMed ID: 37018380

Doi: 10.1371/journal.ppat.1011206

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Knockdown of the salivary protein gene NlG14 caused displacement of the lateral oviduct secreted components and inhibited ovulation in Nilaparvata lugens

Authors: Haoli Gao, Huihui Zhang, Xiaowei Yuan, Xumin Lin, Jianzheng Zou, Na Yu, Zewen Liu

Journal: PLOS Genetics

PubMed ID: 37011098

Doi: 10.1371/journal.pgen.1010704

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Precise integration of large DNA sequences in plant genomes using PrimeRoot editors

Authors: Chao Sun, Yuan Lei, Boshu Li, Qiang Gao, Yunjia Li, Wen Cao, Chao Yang, Hongchao Li, Zhiwei Wang, Yan Li, Yanpeng Wang, Jun Liu, Kevin Tianmeng Zhao, Caixia Gao

Journal: Nature Biotechnology

PubMed ID: 37095350

Doi: 10.1038/s41587-023-01769-w

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Protein Delivery to Insect Epithelial Cells In Vivo: Potential Application to Functional Molecular Analysis of Proteins in Butterfly Wing Development

Authors: Yugo Nakazato, Joji M Otaki

Journal: BioTech (Basel)

PubMed ID: 37092472

Doi: 10.3390/biotech12020028

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Is Penguin Circovirus Circulating Only in the Antarctic Circle? Lack of Viral Detection in Namibia

Authors: Laura C Roberts, Umberto Molini, Lauren M Coetzee, Siegfried Khaiseb, Jean-Paul Roux, Jessica Kemper, David G Roberts, Katrin Ludynia, Marcus Doherr, Darrell Abernethy, Giovanni Franzo

Journal: Animals (Basel)

PubMed ID: 37174486

Doi: 10.3390/ani13091449

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Novel targeting assay uncovers targeting information within peroxisomal ABC transporter Pxa1

Authors: Renate L M Jansen, Marco van den Noort, Arjen M Krikken, Chen Bibi, Astrid Böhm, Maya Schuldiner, Einat Zalckvar, Ida J van der Klei

Journal: Biochim Biophys Acta Mol Cell Res

PubMed ID: 37028652

Doi: 10.1016/j.bbamcr.2023.119471

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A novel anti-membrane CD30 single-chain variable fragment discovered from the human phage library: A potential targeted immunotherapy

Authors: Thanida Chanpong, Watee Seesuay, Wararat Chiangjong, Piamsiri Jiramornimit, Sarinthip Preedagasamzin, Korakot Atjanasuppat, Bunyada Jittorntrum, Somsak Prasongtanakij, Supannikar Tawinwung, Sulada Pukiat, Chonticha Saisawang, Suparerk Borwornpinyo, Khanit Sa-Ngiamsuntorn, Wanpen Chaichumpa, Suradej Hongeng, Usanarat Anurathapan

Journal: PLoS ONE

PubMed ID: 37079526

Doi: 10.1371/journal.pone.0284708

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FTO promotes cervical cancer cell proliferation, colony formation, migration and invasion via the regulation of the BMP4/Hippo/YAP1/TAZ pathway

Authors: Jinyuan Huang, Jing Yang, Yudi Zhang, Dan Lu, Yinmei Dai

Journal: Exp Cell Res

PubMed ID: 37030332

Doi: 10.1016/j.yexcr.2023.113585

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Chemical shift assignments of calmodulin bound to a cytosolic domain of GluN2A (residues 1004-1024) from the NMDA receptor

Authors: Aritra Bej, James B Ames

Journal: Biomol NMR Assign

PubMed ID: 37029330

Doi: 10.1007/s12104-023-10125-7

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Constitutively activated AMPKα1 protects against skeletal aging in mice by promoting bone-derived IGF-1 secretion

Authors: Yiqi Yang, Kai Yuan, Yihao Liu, Qishan Wang, Yixuan Lin, Shengbing Yang, Kai Huang, Tianyou Kan, Yuxin Zhang, Mingming Xu, Zhifeng Yu, Qiming Fan, Yugang Wang, Hanjun Li, Tingting Tang

Journal: Cell Prolif

PubMed ID: 37042047

Doi: 10.1111/cpr.13476

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ATG8-dependent LMX1B-autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience

Authors: Natalia Jiménez-Moreno, Madhu Kollareddy, Petros Stathakos, Joanna J Moss, Zuriñe Antón, Deborah K Shoemark, Richard B Sessions, Ralph Witzgall, Maeve Caldwell, Jon D Lane

Journal: J Cell Biol

PubMed ID: 37014324

Doi: 10.1083/jcb.201910133

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Tuning the immune response: sulfated archaeal glycolipid archaeosomes as an effective vaccine adjuvant for induction of humoral and cell-mediated immunity towards the SARS-CoV-2 Omicron variant of concern

Authors: Tyler M Renner, Bassel Akache, Matthew Stuible, Nazanin Rohani, Yuneivy Cepero-Donates, Lise Deschatelets, Renu Dudani, Blair A Harrison, Jason Baardsnes, Izel Koyuturk, Jennifer J Hill, Usha D Hemraz, Sophie Régnier, Anne E G Lenferink, Yves Durocher, Michael J McCluskie

Journal: Frontiers in Immunology

PubMed ID: 37122746

Doi: 10.3389/fimmu.2023.1182556

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Baseline corticosterone levels in spadefoot toads reflect alternate larval diets one year later

Authors: Cristina C Ledón-Rettig, Katie M Lo, Sarah R Lagon

Journal: Gen Comp Endocrinol

PubMed ID: 37094616

Doi: 10.1016/j.ygcen.2023.114291

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