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Production of the antidepressant orcinol glucoside in Yarrowia lipolytica with yields over 6,400-fold higher than plant extraction

Authors: Bihuan Chen , Xiaonan Liu , Yina Wang , Jie Bai , Xiangyu Liu , Guisheng Xiang , Wei Liu , Xiaoxi Zhu , Jian Cheng , Lina Lu , Guanghui Zhang , Ge Zhang, Zongjie Dai, Shuhui Zi , Shengchao Yang, Huifeng Jiang

Journal: PLoS Biol .

PubMed ID: 37279234

Doi: 10.1371/journal.pbio.3002131

Products & Services: Codon Optimization

Discovery of deaminase functions by structure-based protein clustering

Authors: Jiaying Huang , Qiupeng Lin , Hongyuan Fei , Zixin He , Hu Xu , Yunjia Li , Kunli Qu , Peng Han , Qiang Gao , Boshu Li , Guanwen Liu , Lixiao Zhang , Jiacheng Hu , Rui Zhang , Erwei Zuo , Yonglun Luo , Yidong Ran , Jin-Long Qiu , Kevin Tianmeng Zhao , Caixia Gao

Journal: Cell .

PubMed ID: 37379837

Doi: 10.1016/j.cell.2023.05.041

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Cell targeting and immunostimulatory properties of a novel Fcγ-receptor-independent agonistic anti-CD40 antibody in rhesus macaques

Authors: Xianglei Yan, Sebastian Ols, Rodrigo Arcoverde Cerveira, Klara Lenart, Fredrika Hellgren, Kewei Ye, Alberto Cagigi, Marcus Buggert, Falk Nimmerjahn, Jesper Falkesgaard Højen, Daniel Parera, Ulrich Pessara, Stephan Fischer, Karin Loré

Journal: Cell Mol Life Sci

PubMed ID: 37353664

Doi: 10.1007/s00018-023-04828-2

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Melatonin attenuates monocrotaline-induced hepatic sinusoidal obstruction syndrome in rats via activation of Sirtuin-3

Authors: Xiaofei Ren, Kui Xu, Jianming Xu, Qiao Mei

Journal: J Biochem Mol Toxicol

PubMed ID: 37350538

Doi: 10.1002/jbt.23422

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Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressor

Authors: Mudit Chaand, Chris Fiore, Brian Johnston, Anthony D'Ippolito, Diane H Moon, John P Carulli, Jeffrey R Shearstone

Journal: Commun Biol

PubMed ID: 37316562

Doi: 10.1038/s42003-023-05025-4

Products & Services: PCR Cloning and Subcloning

ATXN3 controls DNA replication and transcription by regulating chromatin structure

Authors: Esperanza Hernández-Carralero, Elisa Cabrera, Gara Rodríguez-Torres, Yeray Hernández-Reyes, Abhay N Singh, Cristina Santa-María, José Miguel Fernández-Justel, Roel C Janssens, Jurgen A Marteijn, Bernd O Evert, Niels Mailand, María Gómez, Kristijan Ramadan, Veronique A J Smits, Raimundo Freire

Journal: Nucleic Acids Res

PubMed ID: 36971114

Doi: 10.1093/nar/gkad212

Products & Services: Monoclonal Antibody Services

Detection of phosphorylation post-translational modifications along single peptides with nanopores

Authors: Ian C Nova, Justas Ritmejeris, Henry Brinkerhoff, Theo J R Koenig, Jens H Gundlach, Cees Dekker

Journal: Nat Biotechnol

PubMed ID: 37386295

Doi: 10.1038/s41587-023-01839-z

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Electrochemical biosensor strategy combining DNA entropy-driven technology to activate CRISPR-Cas13a activity and triple-stranded nucleic acids to detect SARS-CoV-2 RdRp gene

Authors: Chenyi Zhuo, Zichun Song, Jiuying Cui, Yuanxun Gong, Qianli Tang, Kai Zhang, Xinlei Song, Xianjiu Liao

Journal: Mikrochim Acta

PubMed ID: 37351704

Doi: 10.1007/s00604-023-05848-2

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Optimization of mammalian expression vector by cis-regulatory element combinations

Authors: Lu-Yu Zhou, Shuang Zhang, Li-Yun Li, Guo-Yu Yang, Lei Zeng

Journal: Mol Genet Genomics

PubMed ID: 37318628

Doi: 10.1007/s00438-023-02042-0

Products & Services: Gene Synthesis

Rise of the RNA machines - self-amplification in mRNA vaccine design

Authors: Jerome D G Comes, Gorben P Pijlman, Tessy A H Hick

Journal: Trends Biotechnol

PubMed ID: 37328401

Doi: 10.1016/j.tibtech.2023.05.007

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Lymphocyte infiltration and antitumoral effect promoted by cytotoxic inflammatory proteins formulated as self-assembling, protein-only nanoparticles

Authors: Carlos Martínez-Torró, Lorena Alba-Castellón, Luis Miguel Carrasco-Díaz, Naroa Serna, Laura Imedio, Alberto Gallardo, Isolda Casanova, Ugutz Unzueta, Esther Vázquez, Ramón Mangues, Antonio Villaverde

Journal: Biomed Pharmacother

PubMed ID: 37276641

Doi: 10.1016/j.biopha.2023.114976

Products & Services: Proteins, Expression, Isolation and Analysis

Phage anti-CBASS protein simultaneously sequesters cyclic trinucleotides and dinucleotides

Authors: Xueli Cao, Yu Xiao, Erin Huiting, Xujun Cao, Dong Li, Jie Ren, Linlin Guan, Yu Wang, Lingyin Li, Joseph Bondy-Denomy, Yue Feng

Journal: biorxiv

PubMed ID: 37398474

Doi: 10.1101/2023.06.01.543220

Products & Services: Proteins, Expression, Isolation and Analysis

Enzyme assembly on nanoparticle scaffolds enhances cofactor recycling and improves coupled reaction kinetics

Authors: Joyce C Breger, Ellen R Goldman, Kimihiro Susumu, Eunkeu Oh, Christopher M Green, Shelby L Hooe, Meghna Thakur, Igor L Medintz, Gregory A Ellis

Journal: Nanoscale

PubMed ID: 37272342

Doi: 10.1039/d3nr00729d

Products & Services: Proteins, Expression, Isolation and Analysis

In Vitro and In Vivo Efficacy of a Stroma-Targeted, Tumor Microenvironment Responsive Oncolytic Adenovirus in Different Preclinical Models of Cancer

Authors: Ana Alfano, Eduardo G A Cafferata, Mariela Gangemi, Alejandro Nicola Candia, Cristian M Malnero, Ismael Bermudez, Mauricio Vargas Lopez, Gregorio David Ríos, Cecilia Rotondaro, Nicasio Cuneo, David T Curiel, Osvaldo L Podhajcer, Maria Veronica Lopez

Journal: Int J Mol Sci

PubMed ID: 37373140

Doi: 10.3390/ijms24129992

Products & Services: Proteins, Expression, Isolation and Analysis

Heme catabolism mediated by heme oxygenase in uninfected interstitial cells enables efficient symbiotic nitrogen fixation in Lotus japonicus nodules

Authors: Yu Zhou, Longlong Wang, Maria Carmen Rubio, Carmen Pérez-Rontomé, Yumiao Zhou, Yongmei Qi, Tao Tian, Weiqing Zhang, Qiuling Fan, Manuel Becana, Deqiang Duanmu

Journal: New Phytol

PubMed ID: 37329247

Doi: 10.1111/nph.19074

Products & Services: Proteins, Expression, Isolation and Analysis

NeuroMabSeq: high volume acquisition, processing, and curation of hybridoma sequences and their use in generating recombinant monoclonal antibodies and scFvs for neuroscience research

Authors: Keith G Mitchell, Belvin Gong, Samuel S Hunter, Diana Burkart-Waco, Clara E Gavira-O'Neill, Kayla M Templeton, Madeline E Goethel, Malgorzata Bzymek, Leah M MacNiven, Karl D Murray, Matthew L Settles, Lutz Froenicke, James S Trimmer

Journal: biorxiv

PubMed ID: 37425915

Doi: 10.1101/2023.06.28.546392

Products & Services: Proteins, Expression, Isolation and Analysis

N-terminal intrinsic disorder is an ancestral feature of Gγ subunits that influences the balance between different Gβγ signaling axes in yeast

Authors: Xinya Su, Yui Tik Pang, Wei Li, J C Gumbart, Joshua Kelley, Matthew Torres

Journal: J Biol Chem

PubMed ID: 37354971

Doi: 10.1016/j.jbc.2023.104947

Products & Services: Peptide Synthesis

Exploration of T cell immune responses by expression of a dominant-negative SHP1 and SHP2

Authors: Julia Taylor, Anna Bulek, Isaac Gannon, Mathew Robson, Evangelia Kokalaki, Thomas Grothier, Callum McKenzie, Mohamed El-Kholy, Maria Stavrou, Charlotte Traynor-White, Wen Chean Lim, Panagiota Panagiotou, Saket Srivastava, Vania Baldan, James Sillibourne, Mathieu Ferrari, Martin Pule, Simon Thomas

Journal: Front Immunol

PubMed ID: 37334382

Doi: 10.3389/fimmu.2023.1119350

Products & Services: Peptide Synthesis

RNA-binding is an ancient trait of the Annexin family

Authors: Sudarshan S Patil, Vipul Panchal, Trude Røstbø, Sofya Romanyuk, Hanne Hollås, Ruth Brenk, Ann Kari Grindheim, Anni Vedeler

Journal: Front Cell Dev Biol

PubMed ID: 37397259

Doi: 10.3389/fcell.2023.1161588

Products & Services: Proteins, Expression, Isolation and Analysis

MAP2 caps tau fibrils and inhibits aggregation

Authors: Michael R Holden, Brad J Krzesinski, Hilary A Weismiller, Justin R Shady, Martin Margittai

Journal: J Biol Chem

PubMed ID: 37286038

Doi: 10.1016/j.jbc.2023.104891

Products & Services: Molecular Biology Reagents

A Novel Conserved Linear Neutralizing Epitope on the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein

Authors: Rong-Hong Hua, Shu-Jian Zhang, Bei Niu, Jin-Ying Ge, Ting Lan, Zhi-Gao Bu

Journal: Microbiol Spectr

PubMed ID: 37306579

Doi: 10.1128/spectrum.01190-23

Products & Services: Molecular Biology Reagents

The E3 Ubiquitin Ligase, CHIP/STUB1, Inhibits Aggregation of Phosphorylated Proteoforms of Microtubule-associated Protein Tau (MAPT)

Authors: Cory M Nadel, Aye C Thwin, Matthew Callahan, Kanghyun Lee, Emily Connelly, Charles S Craik, Daniel R Southworth, Jason E Gestwicki

Journal: J Mol Biol

PubMed ID: 37330289

Doi: 10.1016/j.jmb.2023.168026

Products & Services: Molecular Biology Reagents

Mutations F352A and Y528A in human HSP90α reduce fibronectin association and fibrillogenesis in cell-derived matrices

Authors: Abir Chakraborty, Ronald Tonui, Adrienne Lesley Edkins

Journal: Cell Stress Chaperones

PubMed ID: 37353709

Doi: 10.1007/s12192-023-01362-9

Products & Services: Proteins, Expression, Isolation and Analysis

Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastoma

Authors: Jasmine Y Lee, Hunter C Jonus, Arhanti Sadanand, Gianna M Branella, Victor Maximov, Suttipong Suttapitugsakul, Matthew J Schniederjan, Jenny Shim, Andrew Ho, Kiran K Parwani, Andrew Fedanov, Adeiye A Pilgrim, Jordan A Silva, Robert W Schnepp, Christopher B Doering, Ronghu Wu, H Trent Spencer, Kelly C Goldsmith

Journal: Cell Rep Med

PubMed ID: 37343516

Doi: 10.1016/j.xcrm.2023.101091

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The full spectrum of OCT1 (SLC22A1) mutations bridges transporter biophysics to drug pharmacogenomics

Authors: Sook Wah Yee, Christian Macdonald, Darko Mitrovic, Xujia Zhou, Megan L Koleske, Jia Yang, Dina Buitrago Silva, Patrick Rockefeller Grimes, Donovan Trinidad, Swati S More, Linda Kachuri, John S Witte, Lucie Delemotte, Kathleen M Giacomini, Willow Coyote-Maestas

Journal: biorxiv

PubMed ID: 37333090

Doi: 10.1101/2023.06.06.543963

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Efficient production of fluorophore-labeled CC chemokines for biophysical studies using recombinant enterokinase and recombinant sortase

Authors: Wenyan Guan, Ning Zhang, Arjan Bains, Mourad Sadqi, Cynthia M Dupureur, Patricia J LiWang

Journal: Biopolymers

PubMed ID: 37341434

Doi: 10.1002/bip.23557

Products & Services: Molecular Biology Reagents

Nullomer peptide increases immune cell infiltration and reduces tumor metabolism in triple negative breast cancer mouse model

Authors: Nilufar Ali, Cody Wolf, Swarna Kanchan, Shivakumar R Veerabhadraiah, Laura Bond, Matthew W Turner, Cheryl L Jorcyk, Greg Hampikian

Journal: Res Sq

PubMed ID: 37461536

Doi: 10.21203/rs.3.rs-3097552/v1

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miR‑124 inhibits cardiomyocyte apoptosis in myocardial ischaemia‑reperfusion injury by activating mitochondrial calcium uniporter regulator 1

Authors: Linlin Guo, Chaoying Liu, Chunyan Jiang, Yanhan Dong, Lynn Htet Htet Aung, Han Ding, Yanyan Gao

Journal: Mol Med Rep

PubMed ID: 37326122

Doi: 10.3892/mmr.2023.13031

Products & Services: Molecular Biology Reagents

Photoperiod Induces DNA Methylation Changes in the Melatonin Receptor 1A Gene in Ewes

Authors: Xiaoyun He, Wei Wang, Wei Sun, Mingxing Chu

Journal: Animals (Basel)

PubMed ID: 37370427

Doi: 10.3390/ani13121917

Products & Services: Proteins, Expression, Isolation and Analysis

Three to four mRNA COVID-19 vaccines in multiple sclerosis patients on immunosuppressive drugs: Seroconversion and variant neutralization

Authors: Céline Louapre, Lisa Belin, Stéphane Marot, Amandine Hippolyte, Edouard Januel, Michella Ibrahim, Lina Jeantin, Karen Zafilaza, Isabelle Malet, Fanny Charbonnier-Beaupel, Michelle Rosenzwajg, Cathia Soulié, Anne-Geneviève Marcelin, Valérie Pourcher

Journal: Eur J Neurol

PubMed ID: 37310391

Doi: 10.1111/ene.15925

Products & Services: Proteins, Expression, Isolation and Analysis

Direct fluorescent labeling of NF186 and NaV16 in living primary neurons using bioorthogonal click chemistry

Authors: Nevena Stajković, Yuanyuan Liu, Aleksandra Arsić, Ning Meng, Hang Lyu, Nan Zhang, Dirk Grimm, Holger Lerche, Ivana Nikić-Spiegel

Journal: J Cell Sci

PubMed ID: 37288813

Doi: 10.1242/jcs.260600

Products & Services: Proteins, Expression, Isolation and Analysis

Identification of a damage-associated molecular pattern (DAMP) receptor and its cognate peptide ligand in sweet potato (Ipomoea batatas)

Authors: Hsueh-Han Lu, Anja K Meents, Judith Fliegmann, Ming-Jing Hwang, Ching-Shu Suen, Diana Masch, Georg Felix, Axel Mithöfer, Kai-Wun Yeh

Journal: Plant Cell Environ

PubMed ID: 37267124

Doi: 10.1111/pce.14633

Products & Services: Peptide Synthesis

Rapid and definitive treatment of phenylketonuria in variant-humanized mice with corrective editing

Authors: Dominique L Brooks, Manuel J Carrasco, Ping Qu, William H Peranteau, Rebecca C Ahrens-Nicklas, Kiran Musunuru, Mohamad-Gabriel Alameh, Xiao Wang

Journal: Nat Commun

PubMed ID: 37301931

Doi: 10.1038/s41467-023-39246-2

Products & Services: Molecular Biology Reagents

Efficient enzymatic synthesis of chiral 2,3-dihydro-1,4-benzodioxane motif using engineered lipase B

Authors: Zhiyun Wu, Weifeng Shi, Ming Jin, Wei Zhou

Journal: RSC Adv

PubMed ID: 37350861

Doi: 10.1039/d3ra02623j

Products & Services: Molecular Biology Reagents

Trypanosoma cruzi Tc24 Antigen Expressed and Orally Delivered by Schizochytrium sp Microalga is Immunogenic in Mice

Authors: Abel Ramos-Vega, Elizabeth Monreal-Escalante, Sergio Rosales-Mendoza, Bernardo Bañuelos-Hernández, Eric Dumonteil, Carlos Angulo

Journal: Mol Biotechnol

PubMed ID: 37344711

Doi: 10.1007/s12033-023-00763-6

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Varicella-zoster virus proteome-wide T-cell screening demonstrates low prevalence of virus-specific CD8 T-cells in latently infected human trigeminal ganglia

Authors: Michiel van Gent, Werner J D Ouwendijk, Victoria L Campbell, Kerry J Laing, Georges M G M Verjans, David M Koelle

Journal: J Neuroinflammation

PubMed ID: 37308917

Doi: 10.1186/s12974-023-02820-y

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Ceramide sensing by human SPT-ORMDL complex for establishing sphingolipid homeostasis

Authors: Tian Xie , Peng Liu , Xinyue Wu , Feitong Dong, Zike Zhang, Jian Yue, Usha Mahawar, Faheem Farooq, Hisham Vohra, Qi Fang, Wenchen Liu, Binks W Wattenberg, Xin Gong

Journal: Nat Commun.

PubMed ID: 37308477

Doi: 10.1038/s41467-023-39274-y

Products & Services: Proteins, Expression, Isolation and Analysis

Persistent serum protein signatures define an inflammatory subcategory of long COVID

Authors: Aarthi Talla, Suhas V Vasaikar, Gregory Lee Szeto, Maria P Lemos, Julie L Czartoski, Hugh MacMillan, Zoe Moodie, Kristen W Cohen, Lamar B Fleming, Zachary Thomson, Lauren Okada, Lynne A Becker, Ernest M Coffey, Stephen C De Rosa, Evan W Newell, Peter J Skene, Xiaojun Li, Thomas F Bumol, M Juliana McElrath, Troy R Torgerson

Journal: Nat Commun

PubMed ID: 37296110

Doi: 10.1038/s41467-023-38682-4

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Protein O-GlcNAcylation homeostasis regulates facultative heterochromatin to fine-tune sog-Dpp signaling during Drosophila early embryogenesis

Authors: Yaowen Zhang, Haibin Yu, Dandan Wang, Xiaoyun Lei, Yang Meng, Na Zhang, Fang Chen, Lu Lv, Qian Pan, Hongtao Qin, Zhuohua Zhang, Daan M F van Aalten, Kai Yuan

Journal: J Genet Genomics

PubMed ID: 37286164

Doi: 10.1016/j.jgg.2023.05.014

Products & Services: Proteins, Expression, Isolation and Analysis

Assessment of Broadly Reactive Responses in Patients With MERS-CoV Infection and SARS-CoV-2 Vaccination

Authors: Hadeel T Zedan, Maria K Smatti, Swapna Thomas, Gheyath K Nasrallah, Nahla M Afifi, Ali Ait Hssain, Laith J Abu Raddad, Peter V Coyle, Jean-Charles Grivel, Muna A Almaslamani, Asmaa A Althani, Hadi M Yassine

Journal: JAMA Netw Open

PubMed ID: 37389876

Doi: 10.1001/jamanetworkopen.2023.19222

Products & Services: Proteins, Expression, Isolation and Analysis

Atomic model for core modifying region of human fatty acid synthase in complex with Denifanstat

Authors: S M Naimul Hasan, Jennifer W Lou, Alexander F A Keszei, David L Dai, Mohammad T Mazhab-Jafari

Journal: Nat Commun

PubMed ID: 37308485

Doi: 10.1038/s41467-023-39266-y

Products & Services: Plasmid DNA Preparation

PGC7 regulates H3K27me3 modification by inhibiting the interaction of YY1 with PRC2

Authors: Weijie Hao, Chenyang Huang, Defu Liu, Hongyu Xu, Yingxiang Liu, Yian Guan, Bingxue Chen, Zekun Guo

Journal: Am J Physiol Cell Physiol

PubMed ID: 37306391

Doi: 10.1152/ajpcell.00041.2023

Three neutralizing mAbs induced by MPXV A29L protein recognizing different epitopes act synergistically against orthopoxvirus

Authors: Mengjun Li, Zuning Ren, Yuelin Wang, Yushan Jiang, Minghui Yang, Delin Li, Jiayin Chen, Zuxin Liang, Yuhao Lin, Zhujun Zeng, Rui Xu, Yiting Wang, Li Zhu, Weiwei Xiao, Qinghua Wu, Bao Zhang, Chengsong Wan, Yang Yang, Bo Wu, Jie Peng, Wei Zhao, Chenguang Shen

Journal: Emerging Microbes & Infections.

PubMed ID: 37288876

Doi: 10.1080/22221751.2023.2223669

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Identification of Anti-Influenza A Compounds Inhibiting the Viral Non-Structural Protein 1 (NS1) Using a Type I Interferon-Driven Screening Strategy

Authors: Giulia Marsili, Chiara Acchioni, Anna Lisa Remoli, Donatella Amatore, Rossella Sgarbanti, Marta De Angelis, Roberto Orsatti, Marta Acchioni, Andrea Astolfi, Nunzio Iraci, Simona Puzelli, Marzia Facchini, Edvige Perrotti, Violetta Cecchetti, Stefano Sabatini, Fabiana Superti, Mariangela Agamennone, Maria Letizia Barreca, John Hiscott, Lucia Nencioni, Marco Sgarbanti

Journal: Int J Mol Sci

PubMed ID: 37445672

Doi: 10.3390/ijms241310495

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The SnRK23-AREB1-TST1/2 cascade activated by cytosolic glucose regulates sugar accumulation across tonoplasts in apple and tomato

Authors: Lingcheng Zhu, Yanzhen Li, Chengcheng Wang, Zhiqi Wang, Wenjing Cao, Jing Su, Yunjing Peng, Baiyun Li, Baiquan Ma, Fengwang Ma, Yong-Ling Ruan, Mingjun Li

Journal: Nat Plants

PubMed ID: 37291399

Doi: 10.1038/s41477-023-01443-8

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Lymphocyte transformation test in the diagnosis of adult fish-induced enterocolitis syndrome

Authors: Ana Entrala, Javier Domínguez-Ortega, Elisa Pulido Lucas, Itsaso Losantos, Magdalena Lluch-Bernal, Santiago Quirce, Rosa Rodríguez-Perez

Journal: J Allergy Clin Immunol Pract

PubMed ID: 37329953

Doi: 10.1016/j.jaip.2023.06.013

Products & Services: Molecular Biology Reagents

LC-MS/MS-Based Absolute Quantitation of Hemoglobin Subunits from Dried Blood Spots Reveals Novel Biomarkers for α-Thalassemia Silent Carriers

Authors: Zhe Ren, Guoying Sun, Qianqian Zhang, Shaomin Zou, Jianhong Chen, Weining Zhao, Guixue Hou, Zeyan Zhong, Jialong Li, Yuhua Ye, Xiangmin Xu, Liang Lin

Journal: Anal Chem

PubMed ID: 37285171

Doi: 10.1021/acs.analchem.3c00895

Products & Services: Molecular Biology Reagents

Hepatic transcriptome analysis provides new insights into ghrelin regulation of the liver in Nile tilapia ()

Authors: Huan Zhong, Chenyi Lou, Bingxin Ren, Jiamin Pi, Tao Dai, Weiling Qin, Yi Zhou

Journal: Front Vet Sci

PubMed ID: 37388466

Doi: 10.3389/fvets.2023.1192195

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Novel mechanisms for the removal of strong replication-blocking HMCES- and thiazolidine-DNA adducts in humans

Authors: Yohei Sugimoto, Yuji Masuda, Shigenori Iwai, Yumi Miyake, Rie Kanao, Chikahide Masutani

Journal: Nucleic Acids Res

PubMed ID: 37021581

Doi: 10.1093/nar/gkad246

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Improved extracellular vesicle-based mRNA delivery for familial hypercholesterolemia treatment

Authors: Zheng Yang, Panpan Ji, Zhelong Li, Rongxin Zhang, Mengying Wei, Yang Yang, Lijun Yuan, Yan Han, Guodong Yang

Journal: Theranostics

PubMed ID: 37351166

Doi: 10.7150/thno.82873

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