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SARS-CoV-2 Serologic Assays Dependent on Dual-Antigen Binding Demonstrate Diverging Kinetics Relative to Other Antibody Detection Methods

Authors: Elitza S Theel, Patrick W Johnson, Katie L Kunze, Liang Wu, Amy P Gorsh, Dane Granger, Matthew M Roforth, Calvin R Jerde, Michael Lasho, Kylie J Andersen, Brenna M Murphy, Julie Harring, Douglas F Lake, Sergei A Svarovsky, Jonathon W Senefeld, Rickey E Carter, Michael J Joyner, Nikola A Baumann, John R Mills

Journal: J Clin Microbiol

PubMed ID: 34166066

Doi: 10.1128/JCM.01231-21

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Phage vB_PaeS-PAJD-1 Rescues Murine Mastitis Infected With Multidrug-Resistant

Authors: Zhaofei Wang, Yibing Xue, Ya Gao, Mengting Guo, Yuanping Liu, Xinwei Zou, Yuqiang Cheng, Jingjiao Ma, Hengan Wang, Jianhe Sun, Yaxian Yan

Journal: Front Cell Infect Microbiol

PubMed ID: 34178726

Doi: 10.3389/fcimb.2021.689770

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Castration-Induced Downregulation of SPARC in Stromal Cells Drives Neuroendocrine Differentiation of Prostate Cancer

Authors: Claudia Enriquez, Valeria Cancila, Renata Ferri, Roberta Sulsenti, Irene Fischetti, Matteo Milani, Paola Ostano, Ilaria Gregnanin, Maurizia Mello-Grand, Enrico Berrino, Marco Bregni, Giuseppe Renne, Claudio Tripodo, Mario P Colombo, Elena Jachetti

Journal: Cancer Res

PubMed ID: 34185677

Doi: 10.1158/0008-5472.CAN-21-0163

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Cell-Surface-Specific Monoclonal Antibodies Protect Mice against Invasive Infection

Authors: Jonothan Rosario-Colon, Karen Eberle, Abby Adams, Evan Courville, Hong Xin

Journal: Int J Mol Sci

PubMed ID: 34200478

Doi: 10.3390/ijms22116162

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Distinct responses of human peripheral blood cells to different misfolded protein oligomers

Authors: Magdalena Leal-Lasarte, Benedetta Mannini, Fabrizio Chiti, Michele Vendruscolo, Christopher M Dobson, Cintia Roodveldt, David Pozo

Journal: Immunology

PubMed ID: 34043816

Doi: 10.1111/imm.13377

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Inhibition of the 3CL Protease and SARS-CoV-2 Replication by Dalcetrapib

Authors: Eric J Niesor, Guy Boivin, Eric Rhéaume, Rong Shi, Véronique Lavoie, Nathalie Goyette, Marie-Eve Picard, Anne Perez, Fouzia Laghrissi-Thode, Jean-Claude Tardif

Journal: ACS Omega

PubMed ID: 34235330

Doi: 10.1021/acsomega.1c01797

Products & Services: Proteins, Expression, Isolation and Analysis

Characterization of an Atypical Hsp70 Demonstrates Its Cytosolic-Nuclear Localization and Modulation by Quercetin and Methylene Blue

Authors: Adélle Burger, Paula Macucule-Tinga, Stephen John Bentley, Michael Hans Ludewig, Ndumiso Nhlakanipho Mhlongo, Addmore Shonhai, Aileen Boshoff

Journal: Int J Mol Sci

PubMed ID: 34202520

Doi: 10.3390/ijms22136776

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Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Authors: Taylor R Jamieson, Joanna Poutou, Ricardo Marius, Xiaohong He, Reza Rezaei, Taha Azad, Carolina S Ilkow

Journal: J Vis Exp

PubMed ID: 34152313

Doi: 10.3791/62486

Products & Services: Proteins, Expression, Isolation and Analysis

Development of a Mini-Replicon-Based Reverse-Genetics System for Rice Stripe Tenuivirus

Authors: Mingfeng Feng, Luyao Li, Ruixiang Cheng, Yulong Yuan, Yongxin Dong, Minglong Chen, Rong Guo, Min Yao, Yi Xu, Yijun Zhou, Jianxiang Wu, Xin Shun Ding, Xueping Zhou, Xiaorong Tao

Journal: J Virol

PubMed ID: 33952642

Doi: 10.1128/JVI.00589-21

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Identification of a dual orange/far-red and blue light photoreceptor from an oceanic green picoplankton

Authors: Yuko Makita, Shigekatsu Suzuki, Keiji Fushimi, Setsuko Shimada, Aya Suehisa, Manami Hirata, Tomoko Kuriyama, Yukio Kurihara, Hidefumi Hamasaki, Emiko Okubo-Kurihara, Kazutoshi Yoshitake, Tsuyoshi Watanabe, Masaaki Sakuta, Takashi Gojobori, Tomoko Sakami, Rei Narikawa, Haruyo Yamaguchi, Masanobu Kawachi, Minami Matsui

Journal: Nat Commun

PubMed ID: 34135337

Doi: 10.1038/s41467-021-23741-5

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miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3

Authors: Fengzhen Liu, Xia Zhang, You Peng, Liping Zhang, Yao Yu, Peng Hua, Peiying Zhu, Xinyu Yan, Yin Li, Liang Zhang

Journal: Cell Rep

PubMed ID: 34107258

Doi: 10.1016/j.celrep.2021.109225

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HIV-1 sequences in lentiviral vector genomes can be substantially reduced without compromising transduction efficiency

Authors: Helin Sertkaya, Mattia Ficarelli, Nathan P Sweeney, Hannah Parker, Conrad A Vink, Chad M Swanson

Journal: Sci Rep

PubMed ID: 34103612

Doi: 10.1038/s41598-021-91309-w

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Soluble Cytoplasmic Expression and Purification of Immunotoxin HER2(scFv)-PE24B as a Maltose Binding Protein Fusion

Authors: Sangsu Park, Minh Quan Nguyen, Huynh Kim Khanh Ta, Minh Tan Nguyen, Gunsup Lee, Chong Jai Kim, Yeon Jin Jang, Han Choe

Journal: Int J Mol Sci

PubMed ID: 34204265

Doi: 10.3390/ijms22126483

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Novel Bacterial Production of Two Different Bioactive Forms of Human Stem-Cell Factor

Authors: Eunyoung Lee, Michelle Novais de Paula, Sangki Baek, Huynh Kim Khanh Ta, Minh Tan Nguyen, Taeck-Hyun Jeong, Chong Jai Kim, Yeon Jin Jang, Han Choe

Journal: Int J Mol Sci

PubMed ID: 34198626

Doi: 10.3390/ijms22126361

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The small G-protein RalA promotes progression and metastasis of triple-negative breast cancer

Authors: Katie A Thies, Matthew W Cole, Rachel E Schafer, Jonathan M Spehar, Dillon S Richardson, Sarah A Steck, Manjusri Das, Arthur W Lian, Alo Ray, Reena Shakya, Sue E Knoblaugh, Cynthia D Timmers, Michael C Ostrowski, Arnab Chakravarti, Gina M Sizemore, Steven T Sizemore

Journal: Breast Cancer Res

PubMed ID: 34118960

Doi: 10.1186/s13058-021-01438-3

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A role for the Cockayne Syndrome B (CSB)-Elongin ubiquitin ligase complex in signal-dependent RNA polymerase II transcription

Authors: Juston C Weems, Brian D Slaughter, Jay R Unruh, Kyle J Weaver, Brandon D Miller, Kym M Delventhal, Joan W Conaway, Ronald C Conaway

Journal: J Biol Chem

PubMed ID: 34116057

Doi: 10.1016/j.jbc.2021.100862

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Machine learning analyses of antibody somatic mutations predict immunoglobulin light chain toxicity

Authors: Maura Garofalo, Luca Piccoli, Margherita Romeo, Maria Monica Barzago, Sara Ravasio, Mathilde Foglierini, Milos Matkovic, Jacopo Sgrignani, Raoul De Gasparo, Marco Prunotto, Luca Varani, Luisa Diomede, Olivier Michielin, Antonio Lanzavecchia, Andrea Cavalli

Journal: Nat Commun

PubMed ID: 34112780

Doi: 10.1038/s41467-021-23880-9

Products & Services: Mammalian Expression

Prdx1 Interacts with ASK1 upon Exposure to HO and Independently of a Scaffolding Protein

Authors: Trung Nghia Vo, Julia Malo Pueyo, Khadija Wahni, Daria Ezeriņa, Jesalyn Bolduc, Joris Messens

Journal: Antioxidants (Basel)

PubMed ID: 34209102

Doi: 10.3390/antiox10071060

Products & Services: Mammalian Expression

Structural basis for SARS-CoV-2 envelope protein recognition of human cell junction protein PALS1

Authors: Jin Chai, Yuanheng Cai, Changxu Pang, Liguo Wang, Sean McSweeney, John Shanklin, Qun Liu

Journal: Nat Commun

PubMed ID: 34103506

Doi: 10.1038/s41467-021-23533-x

Products & Services: PCR Cloning and Subcloning

Novel Bisubstrate Inhibitors for Protein N-Terminal Acetyltransferase D

Authors: Youchao Deng, Sunbin Deng, Yi-Hsun Ho, Sarah M Gardner, Zhi Huang, Ronen Marmorstein, Rong Huang

Journal: J Med Chem

PubMed ID: 34110812

Doi: 10.1021/acs.jmedchem.1c00141

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The Bone Regeneration Capacity of BMP-2 + MMP-10 Loaded Scaffolds Depends on the Tissue Status

Authors: Patricia Garcia-Garcia, Ricardo Reyes, José Antonio Rodriguez, Tomas Martín, Carmen Evora, Patricia Díaz-Rodríguez, Araceli Delgado

Journal: Pharmaceutics

PubMed ID: 34209593

Doi: 10.3390/pharmaceutics13070979

Products & Services: Recombinant Proteins

CircRNA NALCN acts as an miR-493-3p sponge to regulate PTEN expression and inhibit glioma progression

Authors: Yi Liu, Simin Chen, Gang Peng, Yiwei Liao, Xuegong Fan, Zuping Zhang, Chenfu Shen

Journal: Cancer Cell Int

PubMed ID: 34112159

Doi: 10.1186/s12935-021-02001-y

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ACE2 interaction with cytoplasmic PDZ protein enhances SARS-CoV-2 invasion

Authors: Qiangmin Zhang, Julia Gefter, W Bruce Sneddon, Tatyana Mamonova, Peter A Friedman

Journal: iScience

PubMed ID: 34189428

Doi: 10.1016/j.isci.2021.102770

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A liposome-displayed hemagglutinin vaccine platform protects mice and ferrets from heterologous influenza virus challenge

Authors: Zachary R Sia, Xuedan He, Ali Zhang, Jann C Ang, Shuai Shao, Amal Seffouh, Wei-Chiao Huang, Michael R D'Agostino, Amir Teimouri Dereshgi, Sambhara Suryaprakash, Joaquin Ortega, Hanne Andersen, Matthew S Miller, Bruce A Davidson, Jonathan F Lovell

Journal: Proc Natl Acad Sci U S A

PubMed ID: 34050027

Doi: 10.1073/pnas.2025759118

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Surface-Shaving Proteomics of Mycobacterium marinum Identifies Biofilm Subtype-Specific Changes Affecting Virulence, Tolerance, and Persistence

Authors: Kirsi Savijoki, Henna Myllymäki, Hanna Luukinen, Lauri Paulamäki, Leena-Maija Vanha-Aho, Aleksandra Svorjova, Ilkka Miettinen, Adyary Fallarero, Teemu O Ihalainen, Jari Yli-Kauhaluoma, Tuula A Nyman, Mataleena Parikka

Journal: mSystems

PubMed ID: 34156290

Doi: 10.1128/mSystems.00500-21

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LINC_00355 promotes gastric cancer progression by upregulating PHF19 expression through sponging miR-15a-5p

Authors: Jishui Zhang, Wenhao Lv, Yagang Liu, Weihua Fu, Baosheng Chen, Qiutong Ma, Xin Gao

Journal: BMC Cancer

PubMed ID: 34078310

Doi: 10.1186/s12885-021-08227-3

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Significant Inactivation of SARS-CoV-2 In Vitro by a Green Tea Catechin, a Catechin-Derivative, and Black Tea Galloylated Theaflavins

Authors: Eriko Ohgitani, Masaharu Shin-Ya, Masaki Ichitani, Makoto Kobayashi, Takanobu Takihara, Masaya Kawamoto, Hitoshi Kinugasa, Osam Mazda

Journal: Molecules

PubMed ID: 34208050

Doi: 10.3390/molecules26123572

Products & Services: Recombinant Proteins

A novel mouse AAV6 hACE2 transduction model of wild-type SARS-CoV-2 infection studied using synDNA immunogens

Authors: Ebony N Gary, Bryce M Warner, Elizabeth M Parzych, Bryan D Griffin, Xizhou Zhu, Nikesh Tailor, Nicholas J Tursi, Mable Chan, Mansi Purwar, Robert Vendramelli, Jihae Choi, Kathy L Frost, Sophia Reeder, Kevin Liaw, Edgar Tello, Ali R Ali, Kun Yun, Yanlong Pei, Sylvia P Thomas, Amira D Rghei, Matthew M Guilleman, Kar Muthumani, Trevor Smith, Sarah K Wootton, Ami Patel, David B Weiner, Darwyn Kobasa

Journal: iScience

PubMed ID: 34124612

Doi: 10.1016/j.isci.2021.102699

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The ORF8 protein of SARS-CoV-2 mediates immune evasion through down-regulating MHC-Ι

Authors: Yiwen Zhang, Yingshi Chen, Yuzhuang Li, Feng Huang, Baohong Luo, Yaochang Yuan, Baijin Xia, Xiancai Ma, Tao Yang, Fei Yu, Jun Liu, Bingfeng Liu, Zheng Song, Jingliang Chen, Shumei Yan, Liyang Wu, Ting Pan, Xu Zhang, Rong Li, Wenjing Huang, Xin He, Fei Xiao, Junsong Zhang, Hui Zhang

Journal: Proc Natl Acad Sci U S A

PubMed ID: 34021074

Doi: 10.1073/pnas.2024202118

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Evolution of KIPPIS as a versatile platform for evaluating intracellularly functional peptide aptamers

Authors: Daiki Kashima, Masahiro Kawahara

Journal: Sci Rep

PubMed ID: 34083659

Doi: 10.1038/s41598-021-91287-z

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Aneuploid senescent cells activate NF-κB to promote their immune clearance by NK cells

Authors: Ruoxi W Wang, Sonia Viganò, Uri Ben-David, Angelika Amon, Stefano Santaguida

Journal: EMBO Rep

PubMed ID: 34105235

Doi: 10.15252/embr.202052032

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The Functional Consequences of the Novel Ribosomal Pausing Site in SARS-CoV-2 Spike Glycoprotein RNA

Authors: Olga A Postnikova, Sheetal Uppal, Weiliang Huang, Maureen A Kane, Rafael Villasmil, Igor B Rogozin, Eugenia Poliakov, T Michael Redmond

Journal: Int J Mol Sci

PubMed ID: 34204305

Doi: 10.3390/ijms22126490

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Dynamics of alternative splicing during somatic cell reprogramming reveals functions for RNA-binding proteins CPSF3, hnRNP UL1, and TIA1

Authors: Claudia Vivori, Panagiotis Papasaikas, Ralph Stadhouders, Bruno Di Stefano, Anna Ribó Rubio, Clara Berenguer Balaguer, Serena Generoso, Anna Mallol, José Luis Sardina, Bernhard Payer, Thomas Graf, Juan Valcárcel

Journal: Genome Biol

PubMed ID: 34082786

Doi: 10.1186/s13059-021-02372-5

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Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell

Authors: Maritza Puray-Chavez, Kyle M LaPak, Travis P Schrank, Jennifer L Elliott, Dhaval P Bhatt, Megan J Agajanian, Ria Jasuja, Dana Q Lawson, Keanu Davis, Paul W Rothlauf, Zhuoming Liu, Heejoon Jo, Nakyung Lee, Kasyap Tenneti, Jenna E Eschbach, Christian Shema Mugisha, Emily M Cousins, Erica W Cloer, Hung R Vuong, Laura A VanBlargan, Adam L Bailey, Pavlo Gilchuk, James E Crowe, Michael S Diamond, D Neil Hayes, Sean P J Whelan, Amjad Horani, Steven L Brody, Dennis Goldfarb, M Ben Major, Sebla B Kutluay

Journal: Cell Rep

PubMed ID: 34214467

Doi: 10.1016/j.celrep.2021.109364

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Effective Killing of Acute Myeloid Leukemia by TIM-3 Targeted Chimeric Antigen Receptor T Cells

Authors: Wen-Hsin Sandy Lee, Zhiyong Ye, Alice M S Cheung, Y P Sharon Goh, Hsueh Ling Janice Oh, Ravisankar Rajarethinam, Siok Ping Yeo, Mun Kuen Soh, Esther Hian Li Chan, Lip Kun Tan, Soo-Yong Tan, Charles Chuah, Wee Joo Chng, John E Connolly, Cheng-I Wang

Journal: Mol Cancer Ther

PubMed ID: 34158344

Doi: 10.1158/1535-7163.MCT-20-0155

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Design, synthesis and in vitro biological evaluation of a novel class of anti-adenovirus agents based on 3-amino-1,2-propanediol

Authors: Sarah Mazzotta, Judith Berastegui-Cabrera, Margarita Vega-Holm, María Del Rosario García-Lozano, Marta Carretero-Ledesma, Francesca Aiello, José Manuel Vega-Pérez, Jerónimo Pachón, Fernando Iglesias-Guerra, Javier Sánchez-Céspedes

Journal: Bioorg Chem

PubMed ID: 34175724

Doi: 10.1016/j.bioorg.2021.105095

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In vitro reconstitution of Sgk3 activation by phosphatidylinositol 3-phosphate

Authors: Daniel Pokorny, Linda Truebestein, Kaelin D Fleming, John E Burke, Thomas A Leonard

Journal: J Biol Chem

PubMed ID: 34181950

Doi: 10.1016/j.jbc.2021.100919

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A phase transition enhances the catalytic activity of SARM1, an NAD glycohydrolase involved in neurodegeneration

Authors: Heather S Loring, Victoria L Czech, Janneke D Icso, Lauren O'Connor, Sangram S Parelkar, Alexandra B Byrne, Paul R Thompson

Journal: Elife

PubMed ID: 34184985

Doi: 10.7554/eLife.66694

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Puma, noxa, p53, and p63 differentially mediate stress pathway induced apoptosis

Authors: Jun Wang, Holly R Thomas, Zhang Li, Nan Cher Florence Yeo, Hannah E Scott, Nghi Dang, Mohammed Iqbal Hossain, Shaida A Andrabi, John M Parant

Journal: Cell Death Dis

PubMed ID: 34193827

Doi: 10.1038/s41419-021-03902-6

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Structural basis of mechano-chemical coupling by the mitotic kinesin KIF14

Authors: Matthieu P M H Benoit, Ana B Asenjo, Mohammadjavad Paydar, Sabin Dhakal, Benjamin H Kwok, Hernando Sosa

Journal: Nat Commun

PubMed ID: 34131133

Doi: 10.1038/s41467-021-23581-3

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Assembly principles and stoichiometry of a complete human kinetochore module

Authors: Kai Walstein, Arsen Petrovic, Dongqing Pan, Birte Hagemeier, Dorothee Vogt, Ingrid R Vetter, Andrea Musacchio

Journal: Sci Adv

PubMed ID: 34193424

Doi: 10.1126/sciadv.abg1037

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Synthetic extracellular matrices with tailored adhesiveness and degradability support lumen formation during angiogenic sprouting

Authors: Jifeng Liu, Hongyan Long, Dagmar Zeuschner, Andreas F B Räder, William J Polacheck, Horst Kessler, Lydia Sorokin, Britta Trappmann

Journal: Nat Commun

PubMed ID: 34099677

Doi: 10.1038/s41467-021-23644-5

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Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota

Authors: Djalma S Lima-Junior, Siddharth R Krishnamurthy, Nicolas Bouladoux, Nicholas Collins, Seong-Ji Han, Erin Y Chen, Michael G Constantinides, Verena M Link, Ai Ing Lim, Michel Enamorado, Christophe Cataisson, Louis Gil, Indira Rao, Taylor K Farley, Galina Koroleva, Jan Attig, Stuart H Yuspa, Michael A Fischbach, George Kassiotis, Yasmine Belkaid

Journal: Cell

PubMed ID: 34166614

Doi: 10.1016/j.cell.2021.05.020

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Directed evolution of AAV accounting for long-term and enhanced transduction of cardiovascular endothelial cells

Authors: Y B Liu, B C Xu, Y T Chen, X Yuan, J Y Liu, T Liu, G Z Du, W Jiang, Y Yang, Y Zhu, L J Chen, B S Ding, Y Q Wei, L Yang

Journal: Mol Ther Methods Clin Dev

PubMed ID: 34485601

Doi: 10.1016/j.omtm.2021.05.015

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A single dose of the SARS-CoV-2 vaccine BNT162b2 elicits Fc-mediated antibody effector functions and T cell responses

Authors: Alexandra Tauzin, Manon Nayrac, Mehdi Benlarbi, Shang Yu Gong, Romain Gasser, Guillaume Beaudoin-Bussières, Nathalie Brassard, Annemarie Laumaea, Dani Vézina, Jérémie Prévost, Sai Priya Anand, Catherine Bourassa, Gabrielle Gendron-Lepage, Halima Medjahed, Guillaume Goyette, Julia Niessl, Olivier Tastet, Laurie Gokool, Chantal Morrisseau, Pascale Arlotto, Leonidas Stamatatos, Andrew T McGuire, Catherine Larochelle, Pradeep Uchil, Maolin Lu, Walther Mothes, Gaston De Serres, Sandrine Moreira, Michel Roger, Jonathan Richard, Valérie Martel-Laferrière, Ralf Duerr, Cécile Tremblay, Daniel E Kaufmann, Andrés Finzi

Journal: Cell Host Microbe

PubMed ID: 34133950

Doi: 10.1016/j.chom.2021.06.001

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Absolute protein assay for the simultaneous quantification of two epoxide hydrolases in rats by mass spectrometry-based targeted proteomics

Authors: Ting Wu, Xiaoyun Xi, Ying Chen, Chao Jiang, Qian Zhang, Guoliang Dai, Yongtao Bai, Weidong Zhang, Ting Ni, Jiandong Zou, Wenzheng Ju, Meijuan Xu

Journal: J Sep Sci

PubMed ID: 34008891

Doi: 10.1002/jssc.202100066

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Sphingomonas sp. KT‑1 PahZ2 Structure Reveals a Role for Conformational Dynamics in Peptide Bond Hydrolysis

Authors: Chad A. BrambleyChad A. Brambley Department of Chemistry, Middle Tennessee State University, 1301 East Main Street, Murfreesboro, Tennessee 37132, United States More by Chad A. Brambley , Tarah J. Yared, Marriah Gonzalez, Amanda L. Jansch, Jamie R. Wallen, Mitch H. Weiland*, and Justin M. Miller*

Journal: The Journal of Physical Chemistry B

PubMed ID: 34060838

Doi: doi.org/10.1021/acs.jpcb.1c01216

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Molecular basis of anticoagulant and anticomplement activity of the tick salivary protein Salp14 and its homologs

Authors: Stepan S. Denisov, Johannes H. Ippel, Elisabetta Castoldi, Ben J. Mans, Tilman M. Hackeng, and Ingrid Dijkgraaf

Journal: Journal of Biological Chemistry

PubMed ID: 34118237

Doi: 10.1016/j.jbc.2021.100865

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Phosphatidylserine externalized on the colonic capillaries as a novel pharmacological target for IBD therapy

Authors: Xuerui Zhang, Lulu Song, Lin Li, Banghui Zhu, Lina Huo, Zhaoqing Hu, Xinran Wang, Jie Wang, Mengyue Gao, Jing Zhang and Zichun Hua

Journal: Signal transduction and targeted therapy

PubMed ID: 34131110

Doi: 10.1038/s41392-021-00626-z

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Aggrecan modulates the expression and phosphorylation of tau in a novel bigenic TauP301L - Acan mouse model

Authors: Schmidt S,Stapf C,Schmutzler S,Lachmann I,Arendt T,Holzer M,Sonntag M,Morawski M

Journal: The European journal of neuroscience

PubMed ID: 32737917

Doi: 10.1111/ejn.14923

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