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Engineering cell morphology by CRISPR interference in Acinetobacter baylyi ADP1

Authors: Jin Luo, Elena Efimova, Daniel Christoph Volke, Ville Santala, Suvi Santala

Journal: Microb Biotechnol

PubMed ID: 36005297

Doi: 10.1111/1751-7915.14133

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CRISPR-Cas9-directed gene tagging using a single integrase-defective lentiviral vector carrying a transposase-based Cas9 off switch

Authors: Emil Aagaard Thomsen, Kristian Alsbjerg Skipper, Sofie Andersen, Didde Haslund, Thomas Wisbech Skov, Jacob Giehm Mikkelsen

Journal: Mol Ther Nucleic Acids

PubMed ID: 36090759

Doi: 10.1016/j.omtn.2022.08.005

Products & Services: Gene Fragments

Expression of ENaC subunits in epithelia

Authors: Gustavo Frindt, Joel R Meyerson, Alexandra Satty, Joseph M Scandura, Lawrence G Palmer

Journal: J Gen Physiol

PubMed ID: 35939271

Doi: 10.1085/jgp.202213124

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Heterologous Expression and Characterization of a Full-length Protozoan Nitroreductase from Leishmania orientalis isolate PCM2

Authors: Panu Pimviriyakul, Yuvarun Kapaothong, Theerapat Tangsupatawat

Journal: Mol Biotechnol

PubMed ID: 36042106

Doi: 10.1007/s12033-022-00556-3

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Sappanone a prevents diabetic kidney disease by inhibiting kidney inflammation and fibrosis the NF-κB signaling pathway

Authors: Zhe Wang, Zhida Chen, Xinyi Wang, Yepeng Hu, Jing Kong, Jiabin Lai, Tiekun Li, Bibi Hu, Yikai Zhang, Xianan Zheng, Xiaoxian Liu, Shengyao Wang, Shu Ye, Qiao Zhou, Chao Zheng

Journal: Front Pharmacol

PubMed ID: 36052141

Doi: 10.3389/fphar.2022.953004

Products & Services: Protein Electrophoresis and Western

Repression of linc01555 up-regulates angiomotin-p130 via the microRNA-122-5p/clic1 axis to impact vasculogenic mimicry-mediated chemotherapy resistance in small cell lung cancer

Authors: Dan Li, YanWei Shen, Hui Ren, Li Wang, Jin Yang, Yuan Wang

Journal: Cell Cycle

PubMed ID: 36045598

Doi: 10.1080/15384101.2022.2112132

Products & Services: Gene Synthesis

Transcriptomic and Phenotypic Analysis of CRISPR/Cas9-Mediated Knockout in Zebrafish

Authors: Qianqian Yan, Wenhao Li, Xiaoting Gong, Ruiqin Hu, Liangbiao Chen

Journal: Genes (Basel)

PubMed ID: 36011351

Doi: 10.3390/genes13081441

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Endothelial Cell Infection by Guinea Pig Cytomegalovirus Is a Lytic or Persistent Infection Depending on Tissue Origin but Requires Viral Pentamer Complex and pp65 Tegument Protein

Authors: K Yeon Choi, Nadia El-Hamdi, Alistair McGregor

Journal: J Virol

PubMed ID: 36000848

Doi: 10.1128/jvi.00831-22

Products & Services: Protein and Antibody Isolation

KIF20A promotes the development of fibrosarcoma via PI3K-Akt signaling pathway

Authors: Zheng Jin, Shuang Tao, Chao Zhang, Damo Xu, Zhenhua Zhu

Journal: Exp Cell Res

PubMed ID: 36037925

Doi: 10.1016/j.yexcr.2022.113322

Products & Services: Protein and Antibody Isolation

Pericentrin interacts with Kinesin-1 to drive centriole motility

Authors: Matthew R Hannaford, Rong Liu, Neil Billington, Zachary T Swider, Brian J Galletta, Carey J Fagerstrom, Christian Combs, James R Sellers, Nasser M Rusan

Journal: J Cell Biol

PubMed ID: 35929834

Doi: 10.1083/jcb.202112097

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In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM

Authors: Cheng-Yu Huang, Piotr Draczkowski, Yong-Sheng Wang, Chia-Yu Chang, Yu-Chun Chien, Yun-Han Cheng, Yi-Min Wu, Chun-Hsiung Wang, Yuan-Chih Chang, Yen-Chen Chang, Tzu-Jing Yang, Yu-Xi Tsai, Kay-Hooi Khoo, Hui-Wen Chang, Shang-Te Danny Hsu

Journal: Nat Commun

PubMed ID: 35986008

Doi: 10.1038/s41467-022-32588-3

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Safety and Humoral and Cellular Immunogenicity of the BNT162b2 SARS-CoV-2 Vaccine in Liver-Transplanted Adolescents Compared to Healthy Adolescents

Authors: Palittiya Sintusek, Supranee Buranapraditkun, Siriporn Khunsri, Varattaya Saengchaisukhonkit, Preeyaporn Vichaiwattana, Donchida Srimuan, Thanunrat Thongmee, Yong Poovorawan

Journal: Vaccines (Basel)

PubMed ID: 36016212

Doi: 10.3390/vaccines10081324

Reduced Sensitivity of Commercial Spike-Specific Antibody Assays after Primary Infection with the SARS-CoV-2 Omicron Variant

Authors: David Niklas Springer, Thomas Perkmann, Claudia Maria Jani, Patrick Mucher, Katja Prüger, Rodrig Marculescu, Elisabeth Reuberger, Jeremy Vann Camp, Marianne Graninger, Christian Borsodi, Josef Deutsch, Oliver Lammel, Stephan Walter Aberle, Elisabeth Puchhammer-Stöckl, Helmuth Haslacher, Eva Höltl, Judith Helene Aberle, Karin Stiasny, Lukas Weseslindtner

Journal: Microbiol Spectr

PubMed ID: 36005839

Doi: 10.1128/spectrum.02129-22

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Pathological tau signatures and nuclear alterations in neurons, astrocytes and microglia in Alzheimer's disease, progressive supranuclear palsy, and dementia with Lewy bodies

Authors: Mauro Montalbano, Lajja Majmundar, Urmi Sengupta, Leiana Fung, Rakez Kayed

Journal: Brain Pathol

PubMed ID: 36054524

Doi: 10.1111/bpa.13112

Products & Services: Protein and Antibody Isolation

Evolution of transcriptional regulation of histidine metabolism in Gram-positive bacteria

Authors: German A Ashniev, Natalia V Sernova, Aleksei E Shevkoplias, Ivan D Rodionov, Irina A Rodionova, Alexey G Vitreschak, Mikhail S Gelfand, Dmitry A Rodionov

Journal: BMC Genomics

PubMed ID: 36008760

Doi: 10.1186/s12864-022-08796-y

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Design of Peptides for Membrane Insertion: The Critical Role of Charge Separation

Authors: Sydney C Povilaitis, Arman Fathizadeh, Molly Kogan, Ron Elber, Lauren J Webb

Journal: J Phys Chem B

PubMed ID: 35997537

Doi: 10.1021/acs.jpcb.2c04615

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Layer-by-Layer Delivery of Multiple Antigens Using Trimethyl Chitosan Nanoparticles as a Malaria Vaccine Candidate

Authors: Yang Xu, Ziyou Zhou, Brad Brooks, Tammy Ferguson, Judy Obliosca, Jing Huang, Izumi Kaneko, Shiroh Iwanaga, Masao Yuda, Yukiko Tsuji, Huitang Zhang, Christina C Luo, Xunqing Jiang, Xiang-Peng Kong, Moriya Tsuji, Christopher K Tison

Journal: Front Immunol

PubMed ID: 36059505

Doi: 10.3389/fimmu.2022.900080

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Aberrant protein expression of Appl1, Sortilin and Syndecan-1 during the biological progression of prostate cancer

Authors: Carmela Martini, Jessica M Logan, Alexandra Sorvina, Colin Gordon, Andrew R Beck, Ben S-Y Ung, Maria C Caruso, Courtney Moore, Ashleigh Hocking, Ian R D Johnson, Ka Lok Li, Litsa Karageorgos, Ashley M Hopkins, Adrian J Esterman, Chelsea Huzzell, Robert D Brooks, Joanna Lazniewska, Shane M Hickey, Christie Bader, Emma Parkinson-Lawrence, Roberto Weigert, Michael J Sorich, Prerna Tewari, Cara Martin, Sharon O'Toole, Mark Bates, Mark Ward, Bashir Mohammed, Helen Keegan, William Watson, Sophie Prendergast, Sheena Heffernan, Sarah NiMhaolcatha, Roisin O'Connor, Victoria Malone, Marguerite Carter, Katie Ryan, Nathan Brady, Andres Clarke, Filip Sokol, Sarita Prabhakaran, Jürgen Stahl, Sonja Klebe, Hemamali Samaratunga, Brett Delahunt, Stavros Selemidis, Kim L Moretti, Lisa M Butler, John J O'Leary, Douglas A Brooks

Journal: Pathology

PubMed ID: 36089417

Doi: 10.1016/j.pathol.2022.08.001

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Disruption of nuclear envelope integrity as a possible initiating event in tauopathies

Authors: Marine Prissette, Wen Fury, Matthew Koss, Claudia Racioppi, Daria Fedorova, Ella Dragileva, Georgia Clarke, Taylor Pohl, John Dugan, Diana Ahrens, Joyce Chiu, Charleen Hunt, Chia-Jen Siao, Tara Young, Arijit Bhowmick, Vitaliy Rogulin, Mathieu Desclaux, Eric Y Hayden, Michael Podgorski, Min Gao, Lynn E Macdonald, David Frendewey, George D Yancopoulos, Brian Zambrowicz

Journal: Cell Rep

PubMed ID: 36001963

Doi: 10.1016/j.celrep.2022.111249

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A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA

Authors: Vincent V Pham, Michael Gao, Jennifer L Meagher, Janet L Smith, Victoria M D'Souza

Journal: Commun Biol

PubMed ID: 35970937

Doi: 10.1038/s42003-022-03734-w

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Revisiting the multisite phosphorylation that produces the M-phase supershift of key mitotic regulators

Authors: Tan Tan, Chuanfen Wu, Boye Liu, Bih-Fang Pan, David H Hawke, Zehao Su, Shuaishuai Liu, Wei Zhang, Ruoning Wang, Sue-Hwa Lin, Jian Kuang

Journal: Mol Biol Cell

PubMed ID: 35976701

Doi: 10.1091/mbc.E22-04-0118

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Targeted inhibition of ubiquitin signaling reverses metabolic reprogramming and suppresses glioblastoma growth

Authors: Rossella Delle Donne, Rosa Iannucci, Laura Rinaldi, Luca Roberto, Maria A Oliva, Emanuela Senatore, Domenica Borzacchiello, Luca Lignitto, Giorgio Giurato, Francesca Rizzo, Assunta Sellitto, Francesco Chiuso, Salvatore Castaldo, Giovanni Scala, Virginia Campani, Valeria Nele, Giuseppe De Rosa, Chiara D'Ambrosio, Corrado Garbi, Andrea Scaloni, Alessandro Weisz, Concetta Ambrosino, Antonella Arcella, Antonio Feliciello

Journal: Commun Biol

PubMed ID: 35918402

Doi: 10.1038/s42003-022-03639-8

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Defining the ultrastructure of the hematopoietic stem cell niche by correlative light and electron microscopy

Authors: Sobhika Agarwala, Keun-Young Kim, Sebastien Phan, Saeyeon Ju, Ye Eun Kong, Guillaume A Castillon, Eric A Bushong, Mark H Ellisman, Owen J Tamplin

Journal: Elife

PubMed ID: 35943143

Doi: 10.7554/eLife.64835

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Genetically Engineered Pigs as Efficient Salivary Gland Bioreactors for Production of Therapeutically Valuable Human Nerve Growth Factor

Authors: Fang Zeng, Sha Liao, Zhe Kuang, Qingchun Zhu, Hengxi Wei, Junsong Shi, Enqin Zheng, Zheng Xu, Sixiu Huang, Linjun Hong, Ting Gu, Jie Yang, Huaqiang Yang, Gengyuan Cai, Stefan Moisyadi, Johann Urschitz, Zicong Li, Zhenfang Wu

Journal: Cells

PubMed ID: 35954224

Doi: 10.3390/cells11152378

Products & Services: Plasmid DNA Preparation

Structural Study of SARS-CoV-2 Antibodies Identifies a Broad-Spectrum Antibody That Neutralizes the Omicron Variant by Disassembling the Spike Trimer

Authors: Wuqiang Zhan, Xiaolong Tian, Xiang Zhang, Shenghui Xing, Wenping Song, Qianying Liu, Aihua Hao, Yuxia Hu, Meng Zhang, Tianlei Ying, Zhenguo Chen, Fei Lan, Lei Sun

Journal: J Virol

PubMed ID: 35924918

Doi: 10.1128/jvi.00480-22

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Screening of six cation exchange resins for high binding capacity, monomer purity and step yield: A case study

Authors: Wenwen Lu, Zhenzhen Zhang, Shengwei Zhang, Ting Zhang, Yan Wan, Yifeng Li

Journal: Protein Expr Purif

PubMed ID: 35970491

Doi: 10.1016/j.pep.2022.106155

Products & Services: Protein Electrophoresis and Western

Cis interactions between CD2 and its ligands on T cells are required for T cell activation

Authors: Bin Li, Yan Lu, Ming-Chao Zhong, Jin Qian, Rui Li, Dominique Davidson, Zhenghai Tang, Kaiwen Zhu, Jérémy Argenty, Anne Gonzalez de Peredo, Bernard Malissen, Romain Roncagalli, André Veillette

Journal: Sci Immunol

PubMed ID: 35930657

Doi: 10.1126/sciimmunol.abn6373

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Mechanistic insights into global suppressors of protein folding defects

Authors: Gopinath Chattopadhyay, Jayantika Bhowmick, Kavyashree Manjunath, Shahbaz Ahmed, Parveen Goyal, Raghavan Varadarajan

Journal: PLoS Genet

PubMed ID: 36037221

Doi: 10.1371/journal.pgen.1010334

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Self-Assembly of Porcine Parvovirus Virus-like Particles and Their Application in Serological Assay

Authors: Yanfei Gao, Haiwei Wang, Shanghui Wang, Mingxia Sun, Zheng Fang, Xinran Liu, Xuehui Cai, Yabin Tu

Journal: Viruses

PubMed ID: 36016450

Doi: 10.3390/v14081828

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A partnership between the lipid scramblase XK and the lipid transfer protein VPS13A at the plasma membrane

Authors: Andrés Guillén-Samander, Yumei Wu, S Sebastian Pineda, Francisco J García, Julia N Eisen, Marianna Leonzino, Berrak Ugur, Manolis Kellis, Myriam Heiman, Pietro De Camilli

Journal: Proc Natl Acad Sci U S A

PubMed ID: 35994651

Doi: 10.1073/pnas.2205425119

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Refolding and characterization of a diabody against Pfs25, a vaccine candidate of Plasmodium falciparum

Authors: Deepak K Jagannath, Ashwathi Valiyaparambil, Vysakh K Viswanath, Manjunath A Hurakadli, Neelagandan Kamariah, Alifia C Jafer, Chhaya Patole, Sabyasachi Pradhan, Naveen Kumar, Anirudha Lakshminarasimhan

Journal: Anal Biochem

PubMed ID: 35944694

Doi: 10.1016/j.ab.2022.114830

Products & Services: PCR Cloning and Subcloning

SOX transcription factors direct TCF-independent WNT/β-catenin responsive transcription to govern cell fate in human pluripotent stem cells

Authors: Shreyasi Mukherjee, David M Luedeke, Leslie McCoy, Makiko Iwafuchi, Aaron M Zorn

Journal: Cell Rep

PubMed ID: 36001974

Doi: 10.1016/j.celrep.2022.111247

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Defective bone repletion in aged Balb/cBy mice was caused by impaired osteoblastic differentiation

Authors: Matilda H-C Sheng, Kin-Hing William Lau, Charles H Rundle, Anar Alsunna, Sean M Wilson, David J Baylink

Journal: J Bone Miner Metab

PubMed ID: 35947191

Doi: 10.1007/s00774-022-01361-3

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Enhancing arginase 2 expression using target site blockers as a strategy to modulate macrophage phenotype

Authors: Chiara De Santi, Frances K Nally, Remsha Afzal, Conor P Duffy, Stephen Fitzsimons, Stephanie L Annett, Tracy Robson, Jennifer K Dowling, Sally-Ann Cryan, Claire E McCoy

Journal: Mol Ther Nucleic Acids

PubMed ID: 36090747

Doi: 10.1016/j.omtn.2022.08.004

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Structure of a nucleosome-bound MuvB transcription factor complex reveals DNA remodelling

Authors: Marios G Koliopoulos , Reyhan Muhammad , Theodoros I Roumeliotis , Fabienne Beuron , Jyoti S Choudhary , Claudio Alfieri

Journal: Nat Commun

PubMed ID: 36038598

Doi: 10.1038/s41467-022-32798-9

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A specific anti-IFITM2 antibody bars the way to SARS-CoV-2 entry into host cells.

Authors: Anna Basile , Carla Zannella , Margot De Marco , Gianluigi Franci , Massimiliano Galdiero , Giuseppina Sanna , Aldo Manzin , Massimiliano Chetta , Maria Caterina Turco , Alessandra Rosati , Liberato Marzullo

Journal: biorxiv

Doi: doi.org/10.1101/2022.08.04.502768

Products & Services: Monoclonal Antibody Services Recombinant Antibody Expression

Omicron BA4/BA5 escape neutralizing immunity elicited by BA1 infection

Authors: Khadija Khan, Farina Karim, Yashica Ganga, Mallory Bernstein, Zesuliwe Jule, Kajal Reedoy, Sandile Cele, Gila Lustig, Daniel Amoako, Nicole Wolter, Natasha Samsunder, Aida Sivro, James Emmanuel San, Jennifer Giandhari, Houriiyah Tegally, Sureshnee Pillay, Yeshnee Naidoo, Matilda Mazibuko, Yoliswa Miya, Nokuthula Ngcobo, Nithendra Manickchund, Nombulelo Magula, Quarraisha Abdool Karim, Anne von Gottberg, Salim S Abdool Karim, Willem Hanekom, Bernadett I Gosnell, Richard J Lessells, Tulio de Oliveira, Mahomed-Yunus S Moosa, Alex Sigal

Journal: Nat Commun

PubMed ID: 35948557

Doi: 10.1038/s41467-022-32396-9

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Chemical shift assignments of calmodulin bound to a C-terminal site (residues 1120-1147) in the β-subunit of a retinal cyclic nucleotide-gated channel (CNGB1)

Authors: Aritra Bej, James B Ames

Journal: Biomol NMR Assign

PubMed ID: 35986879

Doi: 10.1007/s12104-022-10101-7

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Deletion of the Plasmodium falciparum exported protein PTP7 leads to Maurer's clefts vesiculation, host cell remodeling defects, and loss of surface presentation of EMP1

Authors: Olivia M S Carmo, Gerald J Shami, Dezerae Cox, Boyin Liu, Adam J Blanch, Snigdha Tiash, Leann Tilley, Matthew W A Dixon

Journal: PLoS Pathog

PubMed ID: 35930605

Doi: 10.1371/journal.ppat.1009882

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Identification and characterization of a novel peptide from rainbow trout (Oncorhynchus mykiss) with antimicrobial activity against Streptococcus iniae

Authors: Joseph F A Varga, Sascha R Brunner, Grant Cheng, Daniel Min, Marc G Aucoin, Andrew C Doxey, Brian Dixon

Journal: Dev Comp Immunol

PubMed ID: 36044968

Doi: 10.1016/j.dci.2022.104518

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Krüppel-like factor 5 rewires NANOG regulatory network to activate human naive pluripotency specific LTR7Ys and promote naive pluripotency

Authors: Zhipeng Ai, Xinyu Xiang, Yangquan Xiang, Iwona Szczerbinska, Yuli Qian, Xiao Xu, Chenyang Ma, Yaqi Su, Bing Gao, Hao Shen, Muhammad Nadzim Bin Ramli, Di Chen, Yue Liu, Jia-Jie Hao, Huck Hui Ng, Dan Zhang, Yun-Shen Chan, Wanlu Liu, Hongqing Liang

Journal: Cell Rep

PubMed ID: 36001968

Doi: 10.1016/j.celrep.2022.111240

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Case report: A novel loss-of-function pathogenic variant in the KCNA1 cytoplasmic N-terminus causing carbamazepine-responsive type 1 episodic ataxia

Authors: Rían W Manville, Richard Sidlow, Geoffrey W Abbott

Journal: Front Neurol

PubMed ID: 36016548

Doi: 10.3389/fneur.2022.975849

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Immunogenicity and Safety of SARS-CoV-2 mRNA Vaccines in a Cohort of Patients With Type 1 Diabetes

Authors: Francesca D'Addio, Gianmarco Sabiu, Vera Usuelli, Emma Assi, Ahmed Abdelsalam, Anna Maestroni, Andy Joe Seelam, Moufida Ben Nasr, Cristian Loretelli, Davide Mileto, Giada Rossi, Ida Pastore, Laura Montefusco, Paola S Morpurgo, Laura Plebani, Antonio Rossi, Enrica Chebat, Andrea M Bolla, Maria Elena Lunati, Chiara Mameli, Maddalena Macedoni, Spinello Antinori, Stefano Rusconi, Maurizio Gallieni, Cesare Berra, Franco Folli, Massimo Galli, Maria Rita Gismondo, Gianvincenzo Zuccotti, Paolo Fiorina

Journal: Diabetes

PubMed ID: 35551366

Doi: 10.2337/db22-0053

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Rapid Point-of-Care Test Kit for Bacterial Vaginosis: Detection of Vaginolysin and Clue Cells Using Paper Strips and a Smartphone

Authors: Devon C Pawley, Emre Dikici, Sapna K Deo, Patricia Raccamarich, Margaret A Fischl, Maria Alcaide, Sylvia Daunert

Journal: Anal Chem

PubMed ID: 35943181

Doi: 10.1021/acs.analchem.2c02094

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Understanding the separation of timescales in bacterial proteasome core particle assembly

Authors: Pushpa Itagi, Anupama Kante, Leonila Lagunes, Eric J Deeds

Journal: Biophys J

PubMed ID: 36016496

Doi: 10.1016/j.bpj.2022.08.022

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IL11 Stimulates IL33 Expression and Proinflammatory Fibroblast Activation across Tissues

Authors: Anissa A Widjaja, Sonia Chothani, Sivakumar Viswanathan, Joyce Wei Ting Goh, Wei-Wen Lim, Stuart A Cook

Journal: Int J Mol Sci

PubMed ID: 36012165

Doi: 10.3390/ijms23168900

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In the footsteps of sea stars: deciphering the catalogue of proteins involved in underwater temporary adhesion

Authors: Morgane Algrain, Elise Hennebert, Philip Bertemes, Ruddy Wattiez, Patrick Flammang, Birgit Lengerer

Journal: Open Biol

PubMed ID: 35975651

Doi: 10.1098/rsob.220103

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Neuroimmune Responses in a New Experimental Animal Model of Cerebral Aspergillosis

Authors: Brianne N Sullivan, Mia A Baggett, Christa Guillory, MaryJane Jones, Chad Steele

Journal: MBio

PubMed ID: 36040029

Doi: 10.1128/mbio.02254-22

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Targeting the extra domain A of fibronectin for cancer therapy with CAR-T cells

Authors: Celia Martín-Otal, Aritz Lasarte-Cia, Diego Serrano, Noelia Casares, Enrique Conde, Flor Navarro, Inés Sánchez-Moreno, Marta Gorraiz, Patricia Sarrión, Alfonso Calvo, Carlos E De Andrea, José Echeveste, Amaia Vilas, Juan Roberto Rodriguez-Madoz, Jesús San Miguel, Felipe Prosper, Sandra Hervas-Stubbs, Juan Jose Lasarte, Teresa Lozano

Journal: J Immunother Cancer

PubMed ID: 35918123

Doi: 10.1136/jitc-2021-004479

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Dual role of pseudogene TMEM198B in promoting lipid metabolism and immune escape of glioma cells

Authors: Ying Zhan, Wei Qiao, Bolong Yi, Xinyu Yang, Miaomiao Li, Lu Sun, Lian Ji, Peng Su, Xin Wang, Furong Zhang, Rui Zhang, Mingjun Gao, Wujun Zhao, Yichen Song

Journal: Oncogene

PubMed ID: 36038663

Doi: 10.1038/s41388-022-02445-0

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