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Guide RNA engineering enables efficient CRISPR editing with a miniature Syntrophomonas palmitatica Cas12f1 nuclease

Authors: Yujue Wang , Yannan Wang ， Deng Pan , Haopeng Yu , Yifei Zhang , Weizhong Chen , Fan Li , Zhaowei Wu , Quanjiang Ji

Journal: Cell Rep

PubMed ID: 36170834

Doi: doi.org/10.1016/j.celrep.2022.111418

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Nilaparvata lugens salivary protein NlG14 triggers defense response in plants

Authors: Haoli Gao , Jianzheng Zou , Xumin Lin , Huihui Zhang , Na Yu , Zewen Liu

Journal: J Exp Bot

PubMed ID: 36056768

Doi: 10.1093/jxb/erac354

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Proposal to Consider Chemical/Physical Microenvironment as a New Therapeutic Off-Target Approach

Authors: Alessandro Giuliani, Stefano Fais

Journal: Pharmaceutics

PubMed ID: 36297518

Doi: 10.3390/pharmaceutics14102084

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Autophagic flux restoration of senescent T cells improves antitumor activity of TCR-engineered T cells

Authors: Chaoting Zhang, Yizhe Sun, Shance Li, Luyan Shen, Xia Teng, Yefei Xiao, Ping Zhou, Zheming Lu

Journal: Clin Transl Immunology

PubMed ID: 36188121

Doi: 10.1002/cti2.1419

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Development of a GalNAc-Tyrosine-Specific Monoclonal Antibody and Detection of Tyrosine -GalNAcylation in Numerous Human Tissues and Cell Lines

Authors: Li Xia, Tiffany R Bellomo, Ruslan Gibadullin, Molly D Congdon, Elijah F Edmondson, Mi Li, Alexander Wlodawer, Crystal Li, J Sebastian Temme, Pavan Patel, Donna Butcher, Jeffrey C Gildersleeve

Journal: J Am Chem Soc

PubMed ID: 36054098

Doi: 10.1021/jacs.2c04477

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Febuxostat Alleviates Allergic Rhinitis by Inhibiting Inflammation and Monocyte Adhesion in Human Nasal Epithelial Cells via Regulating KLF6

Authors: Yuting Yao, Ran Wei, Hui Jiang

Journal: Evid Based Complement Alternat Med

PubMed ID: 36118091

Doi: 10.1155/2022/9092311

Products & Services: Gene Synthesis

Mitochondrial derived peptide MOTS-c prevents the development of heart failure under pressure overload conditions in mice

Authors: Peng Zhong, Jianye Peng, Yewen Hu, Jun Zhang, Caijie Shen

Journal: J Cell Mol Med

PubMed ID: 36156853

Doi: 10.1111/jcmm.17551

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Simple and practical sialoglycan encoding system reveals vast diversity in nature and identifies a universal sialoglycan-recognizing probe derived from AB5 toxin B subunits

Authors: Aniruddha Sasmal, Naazneen Khan, Zahra Khedri, Benjamin P Kellman, Saurabh Srivastava, Andrea Verhagen, Hai Yu, Anders Bech Bruntse, Sandra Diaz, Nissi Varki, Travis Beddoe, Adrienne W Paton, James C Paton, Xi Chen, Nathan E Lewis, Ajit Varki

Journal: Glycobiology

PubMed ID: 36048714

Doi: 10.1093/glycob/cwac057

Products & Services: ORF cDNA Clones/MolecularCloud

Evidence of Orientation-Dependent Early States of Prion Protein Misfolded Structures from Single Molecule Force Spectroscopy

Authors: Andrea Raspadori, Valentina Vignali, Anna Murello, Gabriele Giachin, Bruno Samorì, Motomasa Tanaka, Carlos Bustamante, Giampaolo Zuccheri, Giuseppe Legname

Journal: Biology (Basel)

PubMed ID: 36138837

Doi: 10.3390/biology11091358

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Metabolic remodeling of pyrimidine synthesis pathway and serine synthesis pathway in human glioblastoma

Authors: Akira Nakamizo, Yuichiro Miyamatsu, Haruka Hirose, Toshiyuki Amano, Satoshi Matsuo, Minako Fujiwara, Teppei Shimamura, Koji Yoshimoto

Journal: Sci Rep

PubMed ID: 36175487

Doi: 10.1038/s41598-022-20613-w

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Body Weight is Inversely Associated with Anti-SARS-CoV-2 Antibody Levels after BNT162b2 mRNA Vaccination in Young and Middle Aged Adults

Authors: Su Youn Nam, Seong Woo Jeon, Deuk Kju Jung, Sung-Jae Heo

Journal: Infect Chemother

PubMed ID: 36196609

Doi: 10.3947/ic.2022.0089

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Antigenic characterization of the SARS-CoV-2 Omicron subvariant BA275

Authors: Qian Wang, Sho Iketani, Zhiteng Li, Yicheng Guo, Andre Yanchen Yeh, Michael Liu, Jian Yu, Zizhang Sheng, Yaoxing Huang, Lihong Liu, David D Ho

Journal: Cell Host Microbe

PubMed ID: 36108630

Doi: 10.1016/j.chom.2022.09.002

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Heterologous Expression and Catalytic Properties of Codon-Optimized Small-Sized Bromelain from MD2 Pineapple

Authors: Rafida Razali, Fikran Aranda Fahrudin, Vijay Kumar Subbiah, Kazufumi Takano, Cahyo Budiman

Journal: Molecules

PubMed ID: 36144767

Doi: 10.3390/molecules27186031

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A nuclear import pathway exploited by pathogenic noncoding RNAs

Authors: Junfei Ma, Shachinthaka D Dissanayaka Mudiyanselage, Woong June Park, Mo Wang, Ryuta Takeda, Bin Liu, Ying Wang

Journal: Plant Cell

PubMed ID: 35877068

Doi: 10.1093/plcell/koac210

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Activated AMP-activated protein kinase prevents hepatic steatosis, oxidative stress and inflammation in primary chicken hepatocytes

Authors: Yao Yao, Longlong Li, Huihui Wang, Ying Yang, Haitian Ma

Journal: Front Physiol

PubMed ID: 36160867

Doi: 10.3389/fphys.2022.974825

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ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation

Authors: Haruka Hiraoka, Jiewen Wang, Tadashi Nakano, Yasuhiro Hirano, Shinichi Yamazaki, Yasushi Hiraoka, Tokuko Haraguchi

Journal: FEBS Open Bio

PubMed ID: 36054629

Doi: 10.1002/2211-5463.13480

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Unveiling the Essential Role of Arkadia's Non-RING Elements in the Ubiquitination Process

Authors: Maria Birkou, Georgia N Delegkou, Konstantinos D Marousis, Nefeli Fragkaki, Tamara Toro, Vasso Episkopou, Georgios A Spyroulias

Journal: Int J Mol Sci

PubMed ID: 36142504

Doi: 10.3390/ijms231810585

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Cutaneous kinase activity correlates with treatment outcomes following PI3K delta inhibition in mice with experimental pemphigoid diseases

Authors: Saeedeh Ghorbanalipoor, Shirin Emtenani, Melissa Parker, Mayumi Kamaguchi, Colin Osterloh, Manuela Pigors, Natalie Gross, Stanislav Khil'chenko, Anika Kasprick, Sabrina Patzelt, Diana Wortmann, Ibrahim O Ibrahim, Kentaro Izumi, Stephanie Goletz, Katharina Boch, Kathrin Kalies, Katja Bieber, Paul Smith, Enno Schmidt, Ralf J Ludwig

Journal: Front Immunol

PubMed ID: 36248903

Doi: 10.3389/fimmu.2022.865241

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A bead-based method for high-throughput mapping of the sequence- and force-dependence of T cell activation

Authors: Yinnian Feng, Xiang Zhao, Adam K White, K Christopher Garcia, Polly M Fordyce

Journal: Nat Methods

PubMed ID: 36064771

Doi: 10.1038/s41592-022-01592-2

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A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range

Authors: Madhusudan Hosamani, Shreya Gopinath, B P Sreenivasa, Subhasmita Behera, Suresh H Basagoudanavar, Ashok Boora, Durlav P Bora, Pankaj Deka, Veerakyathappa Bhanuprakash, Raj Kumar Singh, Aniket Sanyal, Klaas Weerdmeester, Aldo Dekker

Journal: Appl Microbiol Biotechnol

PubMed ID: 36089639

Doi: 10.1007/s00253-022-12151-2

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Synthetic evolution of herbicide resistance using a T7 RNAP-based random DNA base editor

Authors: Haroon Butt, Jose Luis Moreno Ramirez, Magdy Mahfouz

Journal: Life Sci Alliance

PubMed ID: 36171140

Doi: 10.26508/lsa.202201538

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Characterization of prefusion-F-specific antibodies elicited by natural infection with human metapneumovirus

Authors: Scott A Rush, Gurpreet Brar, Ching-Lin Hsieh, Emilie Chautard, Jennifer N Rainho-Tomko, Chris D Slade, Christine A Bricault, Ana Kume, James Kearns, Rachel Groppo, Sophia T Mundle, Linong Zhang, Danilo Casimiro, Tong-Ming Fu, Joshua M DiNapoli, Jason S McLellan

Journal: Cell Rep

PubMed ID: 36130517

Doi: 10.1016/j.celrep.2022.111399

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Role of lipopolysaccharide in antimicrobial and cell penetrating peptide membrane interactions probed by deuterium NMR of whole cells

Authors: Sarika Kumari, Michael R Morrow, Valerie Booth

Journal: Biochim Biophys Acta Biomembr

PubMed ID: 36155053

Doi: 10.1016/j.bbamem.2022.184053

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Immunoglobulin yolk targeting spike 1, receptor binding domain of spike glycoprotein and nucleocapsid of SARS-CoV-2 blocking RBD-ACE2 binding interaction

Authors: Meliana Eka Saputri, Siti Aisyah Rahmalia Effendi, Rifa Nadila, Syauqi Azzam Fajar, Retno Damajanti Soejoedono, Ekowati Handharyani, Okti Nadia Poetri

Journal: Int Immunopharmacol

PubMed ID: 36183680

Doi: 10.1016/j.intimp.2022.109280

Splice-switch oligonucleotide-based combinatorial platform prioritizes synthetic lethal targets CHK1 and BRD4 against MYC-driven hepatocellular carcinoma

Authors: Dexter Kai Hao Thng, Tan Boon Toh, Paolo Pigini, Lissa Hooi, Yock Young Dan, Pierce Kah-Hoe Chow, Glenn Kunnath Bonney, Masturah Bte Mohd Abdul Rashid, Ernesto Guccione, Dave Keng Boon Wee, Edward Kai-Hua Chow

Journal: Bioeng Transl Med

PubMed ID: 36684069

Doi: 10.1002/btm2.10363

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Personalized neoantigen vaccine combined with PD-1 blockade increases CD8+ tissue-resident memory T-cell infiltration in preclinical hepatocellular carcinoma models

Authors: Hengkai Chen, Zhenli Li, Liman Qiu, Xiuqing Dong, Geng Chen, Yingjun Shi, Linsheng Cai, Wenhan Liu,Honghao Ye,Yang Zhou,Jiahe Ouyang, Zhixiong Cai and Xiaolong Liu

Journal: J Immunother Cancer

PubMed ID: 36113894

Doi: 10.1136/jitc-2021-004389

Products & Services: Peptide Synthesis

Asymptomatic or symptomatic SARS-CoV-2 infection plus vaccination confers increased adaptive immunity to variants of concern

Authors: Peifang Sun, Irene Ramos, Camila H Coelho, Alba Grifoni, Corey A Balinsky, Sindhu Vangeti, Alison Tarke, Nathaniel I Bloom, Vihasi Jani, Silvia J Jakubski, David A Boulifard, Elizabeth Cooper, Carl W Goforth, Jan Marayag, Amethyst Marrone, Edgar Nunez, Lindsey White, Chad K Porter, Victor A Sugiharto, Megan Schilling, Avinash S Mahajan, Charmagne Beckett, Alessandro Sette, Stuart C Sealfon, Shane Crotty, Andrew G Letizia

Journal: iScience

PubMed ID: 36168391

Doi: 10.1016/j.isci.2022.105202

Products & Services: Gene Synthesis

Overexpression of NDST1 Attenuates Fibrotic Response in Murine Adipose-Derived Stem Cells

Authors: Takayoshi Otsuka, Ho-Man Kan, Timothy D Mason, Lakshmi S Nair, Cato T Laurencin

Journal: Stem Cells Dev

PubMed ID: 35920108

Doi: 10.1089/scd.2022.0053

Products & Services: Plasmid DNA Preparation

Disruption of grin2B, an ASD-associated gene, produces social deficits in zebrafish

Authors: Josiah D Zoodsma, Emma J Keegan, Gabrielle R Moody, Ashwin A Bhandiwad, Amalia J Napoli, Harold A Burgess, Lonnie P Wollmuth, Howard I Sirotkin

Journal: Mol Autism

PubMed ID: 36138431

Doi: 10.1186/s13229-022-00516-3

Products & Services: Gene Synthesis

Development and optimization of biologically contained Marburg virus for high-throughput antiviral screening

Authors: Bert Vanmechelen, Joren Stroobants, Winston Chiu, Lieve Naesens, Joost Schepers, Kurt Vermeire, Piet Maes

Journal: Antiviral Res

PubMed ID: 36183903

Doi: 10.1016/j.antiviral.2022.105426

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Bombyx mori transcription factor, E74A, beneficially affects BmNPV infection through direct interaction

Authors: Xin Wang, Jun-Li Lv, Shuang Cheng, Zhi-Hao Su, Sheng Qin, Xia Sun, Xu-Dong Tang, Qiu-Ning Liu, Mu-Wang Li, Xue-Yang Wang

Journal: Pest Manag Sci

PubMed ID: 36054174

Doi: 10.1002/ps.7152

Products & Services: Molecular Biology Tools

Utilization of the Abbott SARS-CoV-2 IgG II Quant Assay To Identify High-Titer Anti-SARS-CoV-2 Neutralizing Plasma against Wild-Type and Variant SARS-CoV-2 Viruses

Authors: Yi-Chan J Lin, David H Evans, Ninette F Robbins, Guillermo Orjuela, Queenie Hu, Reuben Samson, Kento T Abe, Bhavisha Rathod, Karen Colwill, Anne-Claude Gingras, Ashleigh Tuite, Qi-Long Yi, Sheila F O'Brien, Steven J Drews

Journal: Microbiol Spectr

PubMed ID: 36125288

Doi: 10.1128/spectrum.02811-22

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Investigation of the Neutralizing Antibody Response of Healthcare Workers at a Korean University Hospital Six Months After the Introduction of the COVID-19 Vaccine

Authors: Seong-Ho Choi, Ji Young Park, Joung Ha Park, Min-Chul Kim, Oh Joo Kweon, Jin-Won Chung

Journal: Ann Lab Med

PubMed ID: 35470282

Doi: 10.3343/alm.2022.42.5.612

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Nanobody-based RFP-dependent Cre recombinase for selective anterograde tracing in RFP-expressing transgenic animals

Authors: Ayumu Inutsuka, Sho Maejima, Hiroyuki Mizoguchi, Ryosuke Kaneko, Rei Nomura, Keiko Takanami, Hirotaka Sakamoto, Tatsushi Onaka

Journal: Commun Biol

PubMed ID: 36114373

Doi: 10.1038/s42003-022-03944-2

Products & Services: Codon Optimization

OsAMT1;1 and OsAMT1;2 Coordinate Root Morphological and Physiological Responses to Ammonium for Efficient Nitrogen Foraging in Rice

Authors: Xiangyu Wu, Xiaoxiao Xie, Shan Yang, Qianyu Yin, Huairong Cao, Xiaonan Dong, Jing Hui, Zhi Liu, Zhongtao Jia, Chuanzao Mao, Lixing Yuan

Journal: Plant&cell physiology

PubMed ID: 35861152

Doi: 10.1093/pcp/pcac104

Products & Services: Antibody Modification and Purification Services

The gene regulates dimorphic sexual and aggressive behaviors in

Authors: Caihong Han, Qionglin Peng, Mengshi Sun, Xinyu Jiang, Xiangbin Su, Jiangtao Chen, Mingze Ma, Huan Zhu, Xiaoxiao Ji, Yufeng Pan

Journal: Proc Natl Acad Sci U S A

PubMed ID: 36067320

Doi: 10.1073/pnas.2201513119

Products & Services: Polyclonal Antibody Services

Accounting for small variations in the tracrRNA sequence improves sgRNA activity predictions for CRISPR screening

Authors: Peter C DeWeirdt, Abby V McGee, Fengyi Zheng, Ifunanya Nwolah, Mudra Hegde, John G Doench

Journal: Nat Commun

PubMed ID: 36068235

Doi: 10.1038/s41467-022-33024-2

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Function identification and characterization of ZRT and IRT-like proteins computationally for nutrition and biofortification in rice

Authors: Khaled Fathy Abdelmotelb Mohammed, Tanushri Kaul, Pawan Kumar Agrawal, Arulprakash Thangaraj, Rashmi Kaul, Sudhir K Sopory

Journal: J Biomol Struct Dyn

PubMed ID: 36111599

Doi: 10.1080/07391102.2022.2118169

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Lymphocyte Subpopulations Associated with Neutralizing Antibody Levels of SARS-CoV-2 for COVID-19 Vaccination

Authors: Wan-Ting Huang, Shao-Wen Weng, Hong-Tai Tzeng, Feng-Chun Yen, Yu-Shao Chiang, Huey-Ling You

Journal: Vaccines (Basel)

PubMed ID: 36146627

Doi: 10.3390/vaccines10091550

Products & Services: Recombinant Proteins

Single-chain insulin analogs threaded by the insulin receptor αCT domain

Authors: Nicholas A Smith, John G Menting, Michael A Weiss, Michael C Lawrence, Brian J Smith

Journal: Biophys J

PubMed ID: 36181268

Doi: 10.1016/j.bpj.2022.09.038

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Oral or intranasal immunization with recombinant Lactobacillus plantarum displaying head domain of Swine Influenza A virus hemagglutinin protects mice from H1N1 virus

Authors: Yufei Zhang, Li Yang, Jiali Zhang, Kun Huang, Xiaomei Sun, Ying Yang, Ting Wang, Qiang Zhang, Zhong Zou, Meilin Jin

Journal: Microb Cell Fact

PubMed ID: 36085207

Doi: 10.1186/s12934-022-01911-4

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Chemoenzymatic Synthesis of SARS-CoV-2 Homogeneous O-Linked Glycopeptides for Exploring Their Inhibition Functions

Authors: Yongheng Rong, Xingyun Wang, Weian Mao, Fang Yuan, Min Chen, Shengjun Wang, Peng George Wang, Zhigang Wu, Yunjiao He, Yun Kong

Journal: ACS Infect Dis

PubMed ID: 36095241

Doi: 10.1021/acsinfecdis.2c00383

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Identification of a UDP-glucosyltransferase conferring deoxynivalenol resistance in Aegilops tauschii and wheat

Authors: Rizky Pasthika Kirana, Kumar Gaurav, Sanu Arora, Gerlinde Wiesenberger, Maria Doppler, Sebastian Michel, Simone Zimmerl, Magdalena Matic, Chinedu E Eze, Mukesh Kumar, Ajla Topuz, Marc Lemmens, Rainer Schuhmacher, Gerhard Adam, Brande B H Wulff, Hermann Buerstmayr, Barbara Steiner

Journal: Plant Biotechnol J

PubMed ID: 36121345

Doi: 10.1111/pbi.13928

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Comparative transcriptome analysis of longissimus dorsi tissues with different intramuscular fat contents from Guangling donkeys

Authors: Wufeng Li, Lixia Qiu, Jiawei Guan, Yutong Sun, Jingwei Zhao, Min Du

Journal: BMC Genomics

PubMed ID: 36085018

Doi: 10.1186/s12864-022-08857-2

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Immunogenicity after a Third COVID-19 mRNA Booster in Solid Cancer Patients Who Previously Received the Primary Heterologous CoronaVac/ChAdOx1 Vaccine

Authors: Sutima Luangdilok, Passakorn Wanchaijiraboon, Nussara Pakvisal, Thiti Susiriwatananont, Nicha Zungsontiporn, Virote Sriuranpong, Panot Sainamthip, Nungruthai Suntronwong, Preeyaporn Vichaiwattana, Nasamon Wanlapakorn, Yong Poovorawan, Nattaya Teeyapun, Suebpong Tanasanvimon

Journal: Vaccines (Basel)

PubMed ID: 36298478

Doi: 10.3390/vaccines10101613

A megadiverse naïve library derived from numerous camelids for efficient and rapid development of VHH antibodies

Authors: Meiniang Wang, Likun Wei, Haitao Xiang, Bingzhao Ren, Xiaopan Liu, Lin Jiang, Naibo Yang, Jiahai Shi

Journal: Anal Biochem

PubMed ID: 36108795

Doi: 10.1016/j.ab.2022.114871

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A gain-of-function filamin A mutation in mouse platelets induces thrombus instability

Authors: Frédéric Adam, Alexandre Kauskot, Lamia Lamrani, Jean Solarz, Christelle Soukaseum, Christelle Repérant, Cécile V Denis, Hana Raslova, Jean-Philippe Rosa, Marijke Bryckaert

Journal: J Thromb Haemost

PubMed ID: 36006037

Doi: 10.1111/jth.15864

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Herpes Simplex Virus 1 Glycoproteins Differentially Regulate the Activity of Costimulatory Molecules and T Cells

Authors: Harry H Matundan, Ujjaldeep Jaggi, Homayon Ghiasi

Journal: mSphere

PubMed ID: 36094100

Doi: 10.1128/msphere.00382-22

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A 1-aminocyclopropane-1-carboxylic-acid (ACC) dipeptide elicits ethylene responses through ACC-oxidase mediated substrate promiscuity

Authors: John Vaughan-Hirsch, Dongdong Li, Albert Roig Martinez, Stijn Roden, Jolien Pattyn, Shu Taira, Hitomi Shikano, Yoko Miyama, Yukari Okano, Arnout Voet, Bram Van de Poel

Journal: Front Plant Sci

PubMed ID: 36172554

Doi: 10.3389/fpls.2022.995073

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A Cephalosporin-Tripodalamine Conjugate Inhibits Metallo-β-Lactamase with High Efficacy and Low Toxicity

Authors: Hongyan Tian, Yiwan Wang, Yang Dai, Anwen Mao, Wanqing Zhou, Xiaoli Cao, Hui Deng, Hao Lu, Lin Ding, Han Shen, Xiaojian Wang

Journal: Antimicrob Agents Chemother

PubMed ID: 36094199

Doi: 10.1128/aac.00352-22

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