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The optimal docking strength for reversibly tethered kinases

Authors: Mateusz Dyla, Nicolás S González Foutel, Daniel E Otzen, Magnus Kjaergaard

Journal: Proc Natl Acad Sci U S A

PubMed ID: 35696590

Doi: 10.1073/pnas.2203098119

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In silico screening and heterologous expression of soluble dimethyl sulfide monooxygenases of microbial origin in Escherichia coli

Authors: Prasanth Karaiyan, Catherine Ching Han Chang, Eng-Seng Chan, Beng Ti Tey, Ramakrishnan Nagasundara Ramanan, Chien Wei Ooi

Journal: Appl Microbiol Biotechnol

PubMed ID: 35713659

Doi: 10.1007/s00253-022-12008-8

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Independent pathways control muscle tissue size and sarcomere remodeling

Authors: David Brooks, Simranjot Bawa, Alexandria Bontrager, Marta Stetsiv, Yungui Guo, Erika R Geisbrecht

Journal: Dev Biol

PubMed ID: 35760368

Doi: 10.1016/j.ydbio.2022.06.014

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Preclinical study of formulated recombinant nucleocapsid protein, the receptor binding domain of the spike protein, and truncated spike (S1) protein as vaccine candidates against COVID-19 in animal models

Authors: Shahram Nazarian, Gholamreza Olad, Raziyeh Abdolhamidi, Mohammad Javad Motamedi, Rouhollah Kazemi, Emad Kordbacheh, Alireza Felagari, Hanieh Olad, Ali Ahmadi, Alireza Bahiraee, Parisa Farahani, Leila Haghighi, Faezeh Hassani, Vahideh Hajhassan, Mona Nadi, Abdolkarim Sheikhi, Jafar Salimian, Jafar Amani

Journal: Mol Immunol

PubMed ID: 35802999

Doi: 10.1016/j.molimm.2022.06.007

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Thymic epithelial cells co-opt lineage-defining transcription factors to eliminate autoreactive T cells

Authors: Daniel A Michelson, Koji Hase, Tsuneyasu Kaisho, Christophe Benoist, Diane Mathis

Journal: Cell

PubMed ID: 35714609

Doi: 10.1016/j.cell.2022.05.018

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Novel Mutations Associated With Epilepsy and Impacts on Neuronal Excitability

Authors: Changning Xie, Fangyun Liu, Hailan He, Fang He, Leilei Mao, Xiaole Wang, Fei Yin, Jing Peng

Journal: Front Mol Neurosci

PubMed ID: 35845605

Doi: 10.3389/fnmol.2022.870182

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EXPLICIT-Kinase: A gene expression predictor for dissecting the functions of the Arabidopsis kinome

Authors: Yuming Peng, Wanzhu Zuo, Hui Zhou, Fenfen Miao, Yu Zhang, Yue Qin, Yi Liu, Yu Long, Shisong Ma

Journal: J Integr Plant Biol

PubMed ID: 35446465

Doi: 10.1111/jipb.13267

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Betaine Alleviates High-Fat Diet-Induced Disruptionof Hepatic Lipid and Iron Homeostasis in Mice

Authors: Yanlin Li, Wenduo Jiang, Yue Feng, Lei Wu, Yimin Jia, Ruqian Zhao

Journal: Int J Mol Sci

PubMed ID: 35682942

Doi: 10.3390/ijms23116263

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Development of a universal antibiotic resistance screening reporter for improving efficiency of cytosine and adenine base editing

Authors: Lixia Ma, Jiani Xing, Qian Li, Zhiying Zhang, Kun Xu

Journal: J Biol Chem

PubMed ID: 35671823

Doi: 10.1016/j.jbc.2022.102103

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Effect of Solvation Conditions on Inter- and Intramolecular Assembly of Full-Length TDP-43

Authors: Abhilasha A Doke, Santosh Kumar Jha

Journal: J Phys Chem B

PubMed ID: 35758053

Doi: 10.1021/acs.jpcb.2c02203

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CRISPR-Cas9 Expressed in Stably Transduced Cell Lines Promotes Recombination and Selects for Herpes Simplex Virus Recombinants

Authors: Hyung Suk Oh, Fernando M Diaz, Changhong Zhou, Nicholas Carpenter, David M Knipe

Journal: Curr Res Virol Sci

PubMed ID: 36330462

Doi: 10.1016/j.crviro.2022.100023

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Structural basis for tunable affinity and specificity of LxCxE-dependent protein interactions with the retinoblastoma protein family

Authors: Sivasankar Putta, Lucia Alvarez, Stephan Lüdtke, Peter Sehr, Gerd A Müller, Samantha M Fernandez, Sarvind Tripathi, Joe Lewis, Toby J Gibson, Lucia B Chemes, Seth M Rubin

Journal: Structure

PubMed ID: 35716663

Doi: 10.1016/j.str.2022.05.019

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Ultrasensitive fluorometric oligonucleotide immunoassay for the simultaneous and efficient detection of two mycotoxins in agricultural products

Authors: Ming Li, Chenfei Han, Chuqin Yang, Mingna Sun, Jinsheng Duan, Xuchun Qiu, Daolin Du

Journal: Anal Methods

PubMed ID: 35583257

Doi: 10.1039/d2ay00367h

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An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations

Authors: Zhike Lu, Ke Ni, Yingying Wang, Yangfan Zhou, Yini Li, Jianfeng Yan, Qingkai Song, Min Liu, Yujun Xu, Zhenxing Yu, Tiannan Guo and Lijia Ma

Journal: Nucleic Acids Res

PubMed ID: 35670669

Doi: 10.1093/nar/gkac458

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Structure basis for inhibition of SARS-CoV-2 by the feline drug GC376

Authors: Xiao-dong Luan, Bin-xian Chen, Wei-juan Shang , Wan-chao Yin , Ye Jin , Lei-ke Zhang , H. Eric Xu and Shu-yang Zhang

Journal: Acta pharmacologica Sinica

Doi: 10.1038/s41401-022-00929-z

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Engineering substrate specificity of HAD phosphatases and multienzyme systems development for the thermodynamic-driven manufacturing sugars

Authors: Chaoyu Tian , Jiangang Yang , Cui Liu , Peng Chen , Tong Zhang , Yan Men , Hongwu Ma , Yuanxia Sun, Yanhe Ma

Journal: Nat Commun

PubMed ID: 35739124

Doi: doi.org/10.1038/s41467-022-31371-8

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WASp modulates RPA function on single-stranded DNA in response to replication stress and DNA damage

Authors: Seong-Su Han , Kuo-Kuang Wen , María L García-Rubio , Marc S Wold , Andrés Aguilera , Wojciech Niedzwiedz , Yatin M Vyas

Journal: Nat Commun

PubMed ID: 35768435

Doi: 10.1038/s41467-022-31415-z.

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Assembly checkpoint of the proteasome regulatory particle is activated by coordinated actions of proteasomal ATPase chaperones

Authors: Asrafun Nahar, Vladyslava Sokolova, Suganya Sekaran, James D Orth, Soyeon Park

Journal: Cell Reports

PubMed ID: 35675778

Doi: 10.1016/j.celrep.2022.110918

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Bioinformatics-Driven Identification of p62 as A Crucial Oncogene in Liver Cancer

Authors: Ling Wang, Culton R Hensley, Mary E Howell, Shunbin Ning

Journal: Front Oncol

PubMed ID: 35814476

Doi: 10.3389/fonc.2022.923009

Products & Services: GenCrispr Cas9 Genome Editing

Rnd3 Expression is Necessary to Maintain Mitochondrial Homeostasis but Dispensable for Autophagy

Authors: Cristina Cueto-Ureña, Enric Mocholí, Josep Escrivá-Fernández, Susana González-Granero, Sabina Sánchez-Hernández, Amalia Solana-Orts, Begoña Ballester-Lurbe, Karim Benabdellah, Rosa M Guasch, José Manuel García-Verdugo, Francisco Martín, Paul J Coffer, Ignacio Pérez-Roger, Enric Poch

Journal: Front Cell Dev Biol

PubMed ID: 35832788

Doi: 10.3389/fcell.2022.834561

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The humoral immune response more than one year after SARS-CoV-2 infection: low detection rate of anti-nucleocapsid antibodies via Euroimmun ELISA

Authors: Gregor Paul, Philipp Strnad, Oliver Wienand, Ursula Krause, Thomas Plecko, Anja Effenberger-Klein, Katrin Elisabeth Giel, Florian Junne, Annette Galante-Gottschalk, Stefan Ehehalt, Jan Steffen Jürgensen

Journal: Infection

PubMed ID: 35648370

Doi: 10.1007/s15010-022-01830-x

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A Nanopore-Based Saccharide Sensor

Authors: Shanyu Zhang, Zhenyuan Cao, Pingping Fan, Yuqin Wang, Wendong Jia, Liying Wang, Kefan Wang, Yao Liu, Xiaoyu Du, Chengzhen Hu, Panke Zhang, Hong-Yuan Chen, Shuo Huang

Journal: Angew Chem Int Ed Engl

PubMed ID: 35718742

Doi: 10.1002/anie.202203769

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Early Life Stage Folic Acid Deficiency Delays the Neurobehavioral Development and Cognitive Function of Rat Offspring by Hindering De Novo Telomere Synthesis

Authors: Dezheng Zhou, Zhenshu Li, Yue Sun, Jing Yan, Guowei Huang and Wen Li

Journal: Int. J. Mol. Sci

Doi: doi.org/10.3390/ijms23136948

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Thymosin β4 increases cardiac cell proliferation, cell engraftment, and the reparative potency of human induced-pluripotent stem cell-derived cardiomyocytes in a porcine model of acute myocardial infarction

Authors: Shi Hua Tan , Sze Jie Loo , Yu Gao , Zhong Hao Tao , Li Ping Su , Chen Xu Wang , Sophia L Zhang , Yong Hui Mu , Ying Hua Cui , Desiree Abdurrachim , Wei Hsin Wang , Janise Lalic , Kheng Choon Lim , Jun Bu , Ru San Tan , Teck Hock Lee , Jianyi Zhang , Lei Ye

Journal: Theranostics

PubMed ID: 34335970

Doi: 10.7150/thno.56757

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Missense mutations affecting Ca-coordination in GCAP1 lead to cone-rod dystrophies by altering protein structural and functional properties

Authors: Giuditta Dal Cortivo, Valerio Marino, Francesco Bonì, Mario Milani, Daniele Dell'Orco

Journal: Biochim Biophys Acta Mol Cell Res

PubMed ID: 32650103

Doi: 10.1016/j.bbamcr.2020.118794

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Screening membraneless organelle participants with machine-learning models that integrate multimodal features

Authors: Zhaoming Chen, Chao Hou, Liang Wang, Chunyu Yu, Taoyu Chen, Boyan Shen, Yaoyao Hou, Pilong Li, Tingting Li

Journal: Proc Natl Acad Sci U S A

PubMed ID: 35687670

Doi: 10.1073/pnas.2115369119

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Codon Optimization Using a Recurrent Neural Network

Authors: Dennis R Goulet, Yongqi Yan, Palak Agrawal, Andrew B Waight, Amanda Nga-Sze Mak, Yi Zhu

Journal: J Comput Biol

PubMed ID: 35727687

Doi: 10.1089/cmb.2021.0458

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Human endogenous retrovirus-K (HERV-K) reverse transcriptase (RT) structure and biochemistry reveals remarkable similarities to HIV-1 RT and opportunities for HERV-K-specific inhibition

Authors: Eric T Baldwin, Matthias Götte, Egor P Tchesnokov, Eddy Arnold, Margit Hagel, Charles Nichols, Pam Dossang, Marieke Lamers, Paul Wan, Stefan Steinbacher, Donna L Romero

Journal: Proc Natl Acad Sci U S A

PubMed ID: 35771941

Doi: 10.1073/pnas.2200260119

Products & Services: Codon Optimization

Comparison of the Immunogenic Properties of Carrying the Mycobacterial Ag85B-ESAT-6 Antigen at Various Cellular Localizations

Authors: Kamilla Wiull, Preben Boysen, Katarzyna Kuczkowska, Lars Fredrik Moen, Harald Carlsen, Vincent G H Eijsink, Geir Mathiesen

Journal: Front Microbiol

PubMed ID: 35722346

Doi: 10.3389/fmicb.2022.900922

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Targeting the interaction of GABA receptors with CaMKII with an interfering peptide restores receptor expression after cerebral ischemia and inhibits progressive neuronal death in mouse brain cells and slices

Authors: Karthik Balakrishnan, Mohammad Hleihil, Musadiq A Bhat, Robert P Ganley, Markus Vaas, Jan Klohs, Hanns Ulrich Zeilhofer, Dietmar Benke

Journal: Brain Pathol

PubMed ID: 35698024

Doi: 10.1111/bpa.13099

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MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells

Authors: Fang Wang, Jürgen van Baal, Lu Ma, Xuejun Gao, Jan Dijkstra, Dengpan Bu

Journal: Anim Nutr

PubMed ID: 35891685

Doi: 10.1016/j.aninu.2022.06.001

Products & Services: Proteins, Expression, Isolation and Analysis

Structural and mechanistic insights into the inhibition of type I-F CRISPR-Cas system by anti-CRISPR protein AcrIF23

Authors: Junhui Ren, Hao Wang, Lingguang Yang, Feixue Li, Yao Wu, Zhipu Luo, Zeliang Chen, Yi Zhang, Yue Feng

Journal: J Biol Chem

PubMed ID: 35697070

Doi: 10.1016/j.jbc.2022.102124

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Immunoinformatics Aided Design and Validation of a Cross-Reactive Peptide Based Multi-Epitope Vaccine Targeting Multiple Serotypes of Dengue Virus

Authors: Vikas Kaushik, Sunil Krishnan G, Lovi Raj Gupta, Utkarsh Kalra, Abdul Rajjak Shaikh, Luigi Cavallo, Mohit Chawla

Journal: Front Immunol

PubMed ID: 35799781

Doi: 10.3389/fimmu.2022.865180

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Recombinant Carrying Endostatin Protein Alleviates Dextran Sodium Sulfate-Induced Colitis and Colon Cancer in Rats

Authors: Zhiqian Bi, Enqing Cui, Yingying Yao, Xiaoyao Chang, Xiaoyang Wang, Yuhui Zhang, Gen-Xing Xu, Hongqin Zhuang, Zi-Chun Hua

Journal: Front Microbiol

PubMed ID: 35847065

Doi: 10.3389/fmicb.2022.927277

Products & Services: Mammalian Expression

CRISPR/CasRx-Mediated RNA Knockdown Reveals That ACE2 Is Involved in the Regulation of Oligodendroglial Cell Morphological Differentiation

Authors: Yukino Kato, Kenji Tago, Shoya Fukatsu, Miyu Okabe, Remina Shirai, Hiroaki Oizumi, Katsuya Ohbuchi, Masahiro Yamamoto, Kazushige Mizoguchi, Yuki Miyamoto, Junji Yamauchi

Journal: Noncoding RNA

PubMed ID: 35736639

Doi: 10.3390/ncrna8030042

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Lipases secreted by a gut bacterium inhibit arbovirus transmission in mosquitoes

Authors: Xi Yu, Liangqin Tong, Liming Zhang, Yun Yang, Xiaoping Xiao, Yibin Zhu, Penghua Wang, Gong Cheng

Journal: PLoS Pathog

PubMed ID: 35679229

Doi: 10.1371/journal.ppat.1010552

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PBP1 plays key roles in sex pheromone reception of the fall armyworm

Authors: Yongzhi Zhong, Minghui Xie, Zhongjuan Di, Fengqi Li, Jiaqi Chen, Xiangxin Kong, Lulu Lin, Weihua Su, Lina Xu, Feng Zhang, Rui Tang, Haoliang Chen

Journal: Int J Biol Macromol

PubMed ID: 35709868

Doi: 10.1016/j.ijbiomac.2022.06.068

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Characterization, expression and function of the pyrokinins (PKs) in the giant freshwater prawn, Macrobrachium rosenbergii

Authors: Tipsuda Thongbuakaew, Sirirak Mukem, Arada Chaiyamoon, Kanjana Khornchatri, Thanapong Kruangkum, Scott F Cummins, Prasert Sobhon

Journal: J Exp Biol

PubMed ID: 35578905

Doi: 10.1242/jeb.243742

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Expression optimization, purification, and biophysical characterization of a GluN2D-containing NMDA receptor

Authors: Aram Chang, Justin M Liu, Katrina Nguyen, P Rajesh Kumar

Journal: Protein Expr Purif

PubMed ID: 35752385

Doi: 10.1016/j.pep.2022.106129

Products & Services: Mammalian Expression

Inactivated whole-virion vaccine BBV152/Covaxin elicits robust cellular immune memory to SARS-CoV-2 and variants of concern

Authors: Rajesh Vikkurthi, Asgar Ansari, Anupama R Pai, Someshwar Nath Jha, Shilpa Sachan, Suvechchha Pandit, Bhushan Nikam, Anurag Kalia, Bimal Prasad Jit, Hilal Ahmad Parray, Savita Singh, Pallavi Kshetrapal, Nitya Wadhwa, Tripti Shrivastava, Poonam Coshic, Suresh Kumar, Pragya Sharma, Nandini Sharma, Juhi Taneja, Anil K Pandey, Ashok Sharma, Ramachandran Thiruvengadam, Alba Grifoni, Daniela Weiskopf, Alessandro Sette, Shinjini Bhatnagar, Nimesh Gupta

Journal: Nat Microbiol

PubMed ID: 35681012

Doi: 10.1038/s41564-022-01161-5

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Long Intergenic Noncoding RNA MIAT as a Regulator of Human Th17 Cell Differentiation

Authors: Mohd Moin Khan, Meraj Hasan Khan, Ubaid Ullah Kalim, Sofia Khan, Sini Junttila, Niklas Paulin, Lingjia Kong, Omid Rasool, Laura L Elo, Riitta Lahesmaa

Journal: Front Immunol

PubMed ID: 35784351

Doi: 10.3389/fimmu.2022.856762

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Glucosamine amends CNS pathology in mucopolysaccharidosis IIIC mouse expressing misfolded HGSNAT

Authors: Xuefang Pan, Mahsa Taherzadeh, Poulomee Bose, Rachel Heon-Roberts, Annie L A Nguyen, TianMeng Xu, Camila Pará, Yojiro Yamanaka, David A Priestman, Frances M Platt, Shaukat Khan, Nidhi Fnu, Shunji Tomatsu, Carlos R Morales, Alexey V Pshezhetsky

Journal: J Exp Med

PubMed ID: 35704026

Doi: 10.1084/jem.20211860

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Increasing the resilience of plant immunity to a warming climate

Authors: Jong Hum Kim, Christian Danve M Castroverde, Shuai Huang, Chao Li, Richard Hilleary, Adam Seroka, Reza Sohrabi, Diana Medina-Yerena, Bethany Huot, Jie Wang, Kinya Nomura, Sharon K Marr, Mary C Wildermuth, Tao Chen, John D MacMicking, Sheng Yang He

Journal: Nature

PubMed ID: 35768511

Doi: 10.1038/s41586-022-04902-y

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TAPBPR employs a ligand-independent docking mechanism to chaperone MR1 molecules

Authors: Andrew C McShan, Christine A Devlin, Georgia F Papadaki, Yi Sun, Adam I Green, Giora I Morozov, George M Burslem, Erik Procko, Nikolaos G Sgourakis

Journal: Nat Chem Biol

PubMed ID: 35725941

Doi: 10.1038/s41589-022-01049-9

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Biosynthetic potential of the global ocean microbiome

Authors: Lucas Paoli, Hans-Joachim Ruscheweyh, Clarissa C Forneris, Florian Hubrich, Satria Kautsar, Agneya Bhushan, Alessandro Lotti, Quentin Clayssen, Guillem Salazar, Alessio Milanese, Charlotte I Carlström, Chrysa Papadopoulou, Daniel Gehrig, Mikhail Karasikov, Harun Mustafa, Martin Larralde, Laura M Carroll, Pablo Sánchez, Ahmed A Zayed, Dylan R Cronin, Silvia G Acinas, Peer Bork, Chris Bowler, Tom O Delmont, Josep M Gasol, Alvar D Gossert, André Kahles, Matthew B Sullivan, Patrick Wincker, Georg Zeller, Serina L Robinson, Jörn Piel, Shinichi Sunagawa

Journal: Nature

PubMed ID: 35732736

Doi: 10.1038/s41586-022-04862-3

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Detection of the SARS-CoV-2 D614G Mutation Using VirSNiP SARS-CoV-2 Spike D614G Assay

Authors: Chih-Chieh Chen, Tze-Kiong Er

Journal: Clin Lab

PubMed ID: 35704738

Doi: 10.7754/Clin.Lab.2021.211009

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Development and Evaluation of Rapid and Accurate CRISPR/Cas13-Based RNA Diagnostics for Pneumonia

Authors: Yangqing Zhan, Xiaoqing Gao, Shaoqiang Li, Yeqi Si, Yuanxiang Li, Xu Han, Wenjun Sun, Zhengtu Li, Feng Ye

Journal: Front Cell Infect Microbiol

PubMed ID: 35782118

Doi: 10.3389/fcimb.2022.904485

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Identification and Characterization of an RRM-Containing, RNA Binding Protein in

Authors: Caterina Ciani, Anna Pérez-Ràfols, Isabelle Bonomo, Mariachiara Micaelli, Alfonso Esposito, Chiara Zucal, Romina Belli, Vito Giuseppe D'Agostino, Irene Bianconi, Vito Calderone, Linda Cerofolini, Orietta Massidda, Michael Bernard Whalen, Marco Fragai, Alessandro Provenzani

Journal: Biomolecules

PubMed ID: 35883478

Doi: 10.3390/biom12070922

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Immunogenicity of candidate SARS-CoV-2 DNA vaccines based on the spike protein

Authors: Heeji Lim, Se Eun Kim, Yun Ha Lee, Yun-Ho Hwang, Su Hwan Kim, Mi Young Kim, Gyung Tae Chung, You-Jin Kim, Dokeun Kim, Jung-Ah Lee

Journal: Virology

PubMed ID: 35751974

Doi: 10.1016/j.virol.2022.06.006

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Anti-spike protein antibody responses to BNT162b2 mRNA vaccine: A single-center survey in a COVID-19 non-epidemic area in Japan

Authors: Sho Mokuda, Namiki Kawanishi, Shintaro Kuroshima, Junko Kono, Hirofumi Nakayama, Hiroshi Mieno, Masashi Kawamoto

Journal: Vaccine X

PubMed ID: 35692460

Doi: 10.1016/j.jvacx.2022.100173

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