| Products/Services Used | Details | Operation |
|---|---|---|
| Protein Electrophoresis and Western | The protein amounts were adjusted across samples, and then 4 × LDS sample buffer (GenScript, M00676-10) was added. Samples were boiled at 95 °C for 5 min. Proteins were separated on GenScript’s SurePAGETM Bis-Tris gels and blotted on the Immobilon-P transfer membrane (Millipore). | Get A Quote |
Testis-specific transcript 10 (Tex10) is highly expressed in the testis, embryonic stem cells (ESCs), and primordial germ cells (PGCs). We previously generated a Tex10 knockout mouse model demonstrating its critical roles in ESC pluripotency and preimplantation development. Here, using conditional knockout mice and dTAG-degron ESCs, we show Tex10 is required for spermatogenesis and ESC-to-PGCLC differentiation. Specifically, Tex10-null spermatocytes arrest at metaphase I, compromising round spermatid formation. Tex10 depletion and overexpression compromise and enhance ESC-to-PGCLC differentiation, respectively. Mechanistically, bulk and single-cell RNA sequencing reveals that Tex10 depletion downregulates genes... More
