CD8+ T cells play a pathogenic role in the development of murine experimental cerebral malaria (ECM) induced by P. berghei ANKA (PbA) infection in C57BL/6 mice. Only a limited number of CD8+ epitopes have been described. Here, we report the identification of a new epitope from the bergheilysin protein recognized by PbA-specific CD8+ T cells. Induction and functionality of these specific CD8+ T cells were investigated in parallel with previously reported epitopes using new tools such as tetramers and reporter cell lines that were developed for this study. We demonstrate that CD8+ T cells of diverse specificities induced during PbA infection share many characteristics. They express cytolytic markers (IFNγ, ... More
CD8+ T cells play a pathogenic role in the development of murine experimental cerebral malaria (ECM) induced by P. berghei ANKA (PbA) infection in C57BL/6 mice. Only a limited number of CD8+ epitopes have been described. Here, we report the identification of a new epitope from the bergheilysin protein recognized by PbA-specific CD8+ T cells. Induction and functionality of these specific CD8+ T cells were investigated in parallel with previously reported epitopes using new tools such as tetramers and reporter cell lines that were developed for this study. We demonstrate that CD8+ T cells of diverse specificities induced during PbA infection share many characteristics. They express cytolytic markers (IFNγ, granzyme B) and chemokine receptors (CXCR3, CCR5) and damage the blood-brain barrier in vivo. Our earlier finding that brain microvessels in mice infected with PbA, but not non-ECM-causing strains, cross-presented a shared epitope was generalizable to these additional epitopes. Suppressing the induction of specific CD8+ T cells through tolerization with high-dose peptide injection was unable to confer protection against ECM, suggesting that CD8+ T cells of other specificities participate in this process. The tools that we developed can be used to further investigate the heterogeneity of CD8+ T cell responses that are involved in ECM.
