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Metabolic Engineering of BD16 for Heterologous Expression of Synthetic Gene Cassette and L-Alanine Production in the Recombinant Strain Using Fed-Batch Fermentation

Authors: Anshula Sharma, Masafumi Noda, Masanori Sugiyama, Baljinder Kaur, Ajaz Ahmad

Journal: Foods

PubMed ID: 34441741

Doi: 10.3390/foods10081964

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miRNA-451 regulates the NF-κB signaling pathway by targeting IKKβ to inhibit glioma cell growth

Authors: Yang Nan, Liyun Guo, Yingwei Zhen, Le Wang, Bingcheng Ren, Xingjie Chen, Yalin Lu, Kai Yu, Yue Zhong, Qiang Huang

Journal: Cell Cycle

PubMed ID: 34463194

Doi: 10.1080/15384101.2021.1969496

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Litopenaeus vannamei peroxiredoxin 2-like is involved in WSSV infection by interaction with wsv089 and VP26

Authors: Xing-Chao Ren, Xiao-Ping Liu, Qing-Hui Liu

Journal: Dev Comp Immunol

PubMed ID: 34450129

Doi: 10.1016/j.dci.2021.104243

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A continuous spectrophotometric assay for glutathione-independent glyoxalases

Authors: Tomiris Mulikova, Zhanibek Bekkhozhin, Aizat Abdirassil, Darkhan Utepbergenov

Journal: Anal Biochem

PubMed ID: 34391725

Doi: 10.1016/j.ab.2021.114317

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Radiation-Induced Phosphorylation of a Prion-Like Domain Regulates Transformation by FUS-CHOP

Authors: Mark Chen, Joseph P Foster, Ian C Lock, Nathan H Leisenring, Andrea R Daniel, Warren Floyd, Eric Xu, Ian J Davis, David G Kirsch

Journal: Cancer Res

PubMed ID: 34385184

Doi: 10.1158/0008-5472.CAN-20-1497

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An ester bond underlies the mechanical strength of a pathogen surface protein

Authors: Hai Lei, Quan Ma, Wenfei Li, Jing Wen, Haibo Ma, Meng Qin, Wei Wang, Yi Cao

Journal: Nat Commun

PubMed ID: 34426584

Doi: 10.1038/s41467-021-25425-6

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Meet the authors: Michael Ranes and Sebastian Guettler

Authors:

Journal: Mol Cell

PubMed ID: 34416135

Doi: 10.1016/j.molcel.2021.08.002

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Dynamics of SARS-CoV-2 Spike Proteins in Cell Entry: Control Elements in the Amino-Terminal Domains

Authors: Enya Qing, Tom Kicmal, Binod Kumar, Grant M Hawkins, Emily Timm, Stanley Perlman, Tom Gallagher

Journal: MBio

PubMed ID: 34340537

Doi: 10.1128/mBio.01590-21

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Deep survey for designing a vaccine against SARS-CoV-2 and its new mutations

Authors: Bahareh Vakili, Ashkan Bagheri, Manica Negahdaripour

Journal: Biologia (Bratisl)

PubMed ID: 34421121

Doi: 10.1007/s11756-021-00866-y

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Compact RNA editors with small Cas13 proteins

Authors: Soumya Kannan, Han Altae-Tran, Xin Jin, Victoria J Madigan, Rachel Oshiro, Kira S Makarova, Eugene V Koonin, Feng Zhang

Journal: Nat Biotechnol

PubMed ID: 34462587

Doi: 10.1038/s41587-021-01030-2

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Structural mechanism of calcium-mediated hormone recognition and Gβ interaction by the human melanocortin-1 receptor

Authors: Shanshan Ma, Yan Chen, Antao Dai, Wanchao Yin, Jia Guo, Dehua Yang, Fulai Zhou, Yi Jiang, Ming-Wei Wang, H Eric Xu

Journal: Cell Res

PubMed ID: 34453129

Doi: 10.1038/s41422-021-00557-y

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Structure of the Human Cholesterol Transporter ABCG1

Authors: Liga Skarda, Julia Kowal, Kaspar P Locher

Journal: J Mol Biol

PubMed ID: 34461069

Doi: 10.1016/j.jmb.2021.167218

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Development of a chimeric protein based on a proteomic approach for the serological diagnosis of human tegumentary leishmaniasis

Authors: Guilherme Caetano Garcia, Ana Maria Ravena Severino Carvalho, Mariana Costa Duarte, Matheus Fernandes Costa E Silva, Fernanda Alvarenga Cardoso Medeiros, Eduardo Antonio Ferraz Coelho, Dênia Monteiro de Moura Franco, Denise Utsch Gonçalves, Tiago Antônio de Oliveira Mendes, Daniel Menezes-Souza

Journal: Appl Microbiol Biotechnol

PubMed ID: 34432132

Doi: 10.1007/s00253-021-11518-1

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Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux

Authors: Lin Lin, Junyu Chen, Ruchira Mitra, Quanxiu Gao, Feiyue Cheng, Tong Xu, Zhenqiang Zuo, Hua Xiang, Jing Han

Journal: Commun Biol

PubMed ID: 34433872

Doi: 10.1038/s42003-021-02541-z

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RhoC Modulates Cell Junctions and Type I Interferon Response in Aggressive Breast Cancers

Authors: Hannah G Abraham, Peter J Ulintz, Laura Goo, Joel A Yates, Andrew C Little, Liwei Bao, Zhifen Wu, Sofia D Merajver

Journal: Front Oncol

PubMed ID: 34513691

Doi: 10.3389/fonc.2021.712041

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Expressing Type I Interferon From Atlantic Salmon Enhances the Innate Antiviral Immune Response and

Authors: Carlos Muñoz, Josue González-Lorca, Mick Parra, Sarita Soto, Natalia Valdes, Ana María Sandino, Rodrigo Vargas, Alex González, Mario Tello

Journal: Front Immunol

PubMed ID: 34475871

Doi: 10.3389/fimmu.2021.696781

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Identifying the Basis for VirS/VirR Two-Component Regulatory System Control of Clostridium perfringens Beta-Toxin Production

Authors: Iman Mehdizadeh Gohari, Jihong Li, Bruce A McClane

Journal: J Bacteriol

PubMed ID: 34228498

Doi: 10.1128/JB.00279-21

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A novel RNA detection technique for point-of-care identification of pathogens

Authors: Mekbib Astatke, Olivia Tiburzi, Amy Connolly

Journal: J Immunoassay Immunochem

PubMed ID: 34355634

Doi: 10.1080/15321819.2021.1955380

Products & Services: Gene Synthesis

HIV-1 Nef interacts with the cyclin K/CDK13 complex to antagonize SERINC5 for optimal viral infectivity

Authors: Qingqing Chai, Sunan Li, Morgan K Collins, Rongrong Li, Iqbal Ahmad, Silas F Johnson, Dylan A Frabutt, Zhichang Yang, Xiaojing Shen, Liangliang Sun, Jian Hu, Judd F Hultquist, B Matija Peterlin, Yong-Hui Zheng

Journal: Cell Rep

PubMed ID: 34380030

Doi: 10.1016/j.celrep.2021.109514

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Rapid selection of HIV envelopes that bind to neutralizing antibody B cell lineage members with functional improbable mutations

Authors: Olivia Swanson, Brianna Rhodes, Avivah Wang, Shi-Mao Xia, Robert Parks, Haiyan Chen, Aja Sanzone, Melissa Cooper, Mark K Louder, Bob C Lin, Nicole A Doria-Rose, Mattia Bonsignori, Kevin O Saunders, Kevin Wiehe, Barton F Haynes, Mihai L Azoitei

Journal: Cell Rep

PubMed ID: 34407396

Doi: 10.1016/j.celrep.2021.109561

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Genome editing with CRISPR/Cas9 in Pinus radiata (D. Don)

Authors: Charleson Poovaiah, Lorelle Phillips, Barbara Geddes, Cathie Reeves, Mathias Sorieul and Glenn Thorlby

Journal: BMC Plant Biology

PubMed ID: 34376154

Doi: 10.1186/s12870-021-03143-x

Products & Services: Gene Synthesis

GRB2 enforces homology-directed repair initiation by MRE11.

Authors: Zu Ye, Shengfeng Xu, Yin Shi, Albino Bacolla, Aleem Syed, Davide Moiani, Chi-Lin Tsai, Qiang Shen, Guang Peng, Paul G Leonard, Darin E Jones, Bin Wang, John A Tainer, Zamal Ahmed

Journal: Sci Adv

Doi: 10.1126/sciadv.abe9254

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Molecular recognition of an acyl-peptide hormone and activation of ghrelin receptor

Authors: Yue Wang, Shimeng Guo, Youwen Zhuang, Ying Yun, Peiyu Xu, Xinheng He, Jia Guo, Wanchao Yin, H Eric Xu, Xin Xie, Yi Jiang

Journal: Nat Commun.

PubMed ID: 34417468

Doi: 10.1038/s41467-021-25364-2

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An EZH2-dependent transcriptional complex promotes aberrant epithelial remodelling after injury

Authors: Huy Q Le, Matthew A Hill, Ines Kollak, Martina Keck, Victoria Schroeder, Johannes Wirth, Wioletta Skronska-Wasek, Eva Schruf, Benjamin Strobel, Heiko Stahl, Franziska E Herrmann, Alexandre R Campos, Jun Li, Karsten Quast, Dagmar Knebel, Coralie Viollet, Matthew J Thomas, David Lamb, James P Garnett

Journal: EMBO Rep

PubMed ID: 34224201

Doi: 10.15252/embr.202152785

Products & Services: Gene Synthesis

Rapid CRISPR/Cas9 Editing of Genotype IX African Swine Fever Virus Circulating in Eastern and Central Africa

Authors: Hussein M Abkallo, Nicholas Svitek, Bernard Oduor, Elias Awino, Sonal P Henson, Samuel O Oyola, Stephen Mwalimu, Nacrya Assad-Garcia, Walter Fuchs, Sanjay Vashee, Lucilla Steinaa

Journal: Front Genet

PubMed ID: 34527025

Doi: 10.3389/fgene.2021.733674

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Three-layered control of mRNA poly(A) tail synthesis in

Authors: Matti Turtola, M Cemre Manav, Ananthanarayanan Kumar, Agnieszka Tudek, Seweryn Mroczek, Paweł S Krawczyk, Andrzej Dziembowski, Manfred Schmid, Lori A Passmore, Ana Casañal, Torben Heick Jensen

Journal: Genes Dev

PubMed ID: 34385261

Doi: 10.1101/gad.348634.121

Products & Services: Bacterial Expression

Comprehensive Proteome and Lysine Acetylome Analysis Reveals the Widespread Involvement of Acetylation in Cold Resistance of Pepper ( L)

Authors: Zhoubin Liu, Jingshuang Song, Wu Miao, Bozhi Yang, Zhuqing Zhang, Wenchao Chen, Fangjun Tan, Huan Suo, Xiongze Dai, Xuexiao Zou, Lijun Ou

Journal: Front Plant Sci

PubMed ID: 34512705

Doi: 10.3389/fpls.2021.730489

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Human Cystathionine γ-Lyase Is Inhibited by -Nitrosation: A New Crosstalk Mechanism between NO and HS

Authors: Dalila G F Fernandes, João Nunes, Catarina S Tomé, Karim Zuhra, João M F Costa, Alexandra M M Antunes, Alessandro Giuffrè, João B Vicente

Journal: Antioxidants (Basel)

PubMed ID: 34573023

Doi: 10.3390/antiox10091391

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DSB-3 reveals conservation and divergence among protein complexes promoting meiotic double-strand breaks

Authors: Albert W Hinman, Hsin-Yi Yeh, Baptiste Roelens, Kei Yamaya, Alexander Woglar, Henri-Marc G Bourbon, Peter Chi, Anne M Villeneuve

Journal: Proc Natl Acad Sci U S A

PubMed ID: 34389685

Doi: 10.1073/pnas.2109306118

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Immune responses in COVID-19 respiratory tract and blood reveal mechanisms of disease severity

Authors: Wuji Zhang, Brendon Chua, Kevin Selva, Lukasz Kedzierski, Thomas Ashhurst, Ebene Haycroft, Suzanne Shoffner, Luca Hensen, David Boyd, Fiona James, Effie Mouhtouris, Jason Kwong, Kyra Chua, George Drewett, Ana Copaescu, Julie Dobson, Louise Rowntree, Jennifer Habel, Lilith Allen, Hui-Fern Koay, Jessica Neil, Matthew Gartner, Christina Lee, Patiyan Andersson, Torsten Seemann, Norelle Sherry, Fatima Amanat, Florian Krammer, Sarah Londrigan, Linda Wakim, Nicholas King, Dale Godfrey, Laura Mackay, Paul Thomas, Suellen Nicholson, Kelly Arnold, Amy Chung, Natasha Holmes, Olivia Smibert, Jason Trubiano, Claire Gordon, Thi Nguyen, Katherine Kedzierska

Journal: Res Sq

PubMed ID: 34462740

Doi: 10.21203/rs.3.rs-802084/v1

Products & Services: Proteins, Expression, Isolation and Analysis

SARS-CoV-2 Isolates Show Impaired Replication in Human Immune Cells but Differential Ability to Replicate and Induce Innate Immunity in Lung Epithelial Cells

Authors: Miao Jiang, Pekka Kolehmainen, Laura Kakkola, Sari Maljanen, Krister Melén, Teemu Smura, Ilkka Julkunen, Pamela Österlund

Journal: Microbiol Spectr

PubMed ID: 34378952

Doi: 10.1128/Spectrum.00774-21

Products & Services: Mammalian Expression

A newly characterized malaria antigen on erythrocyte and merozoite surfaces induces parasite inhibitory antibodies

Authors: Ian C Michelow, Sangshin Park, Shu-Whei Tsai, Bonnie Rayta, Charisse Flerida A Pasaje, Sara Nelson, Angela M Early, Anne P Frosch, George Ayodo, Dipak K Raj, Christina E Nixon, Christian P Nixon, Sunthorn Pond-Tor, Jennifer F Friedman, Michal Fried, Patrick E Duffy, Karine G Le Roch, Jacquin C Niles, Jonathan D Kurtis

Journal: J Exp Med

PubMed ID: 34342640

Doi: 10.1084/jem.20200170

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compensates for in mice in the amino-terminal acetylation pathway

Authors: Hyae Yon Kweon, Mi-Ni Lee, Max Dorfel, Seungwoon Seo, Leah Gottlieb, Thomas PaPazyan, Nina McTiernan, Rasmus Ree, David Bolton, Andrew Garcia, Michael Flory, Jonathan Crain, Alison Sebold, Scott Lyons, Ahmed Ismail, Elaine Marchi, Seong-Keun Sonn, Se-Jin Jeong, Sejin Jeon, Shinyeong Ju, Simon J Conway, Taesoo Kim, Hyun-Seok Kim, Cheolju Lee, Tae-Young Roh, Thomas Arnesen, Ronen Marmorstein, Goo Taeg Oh, Gholson J Lyon

Journal: Elife

PubMed ID: 34355692

Doi: 10.7554/eLife.65952

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Conformational equilibria in allosteric control of Hsp70 chaperones

Authors: Wei Wang, Qinglian Liu, Qun Liu, Wayne A Hendrickson

Journal: Mol Cell

PubMed ID: 34453889

Doi: 10.1016/j.molcel.2021.07.039

Products & Services: Proteins, Expression, Isolation and Analysis

Activated Alpha 2-Macroglobulin Is a Novel Mediator of Mesangial Cell Profibrotic Signaling in Diabetic Kidney Disease

Authors: Jackie Trink, Renzhong Li, Yaseelan Palarasah, Stéphan Troyanov, Thomas E Andersen, Johannes J Sidelmann, Mark D Inman, Salvatore V Pizzo, Bo Gao, Joan C Krepinsky

Journal: Biomedicines

PubMed ID: 34572299

Doi: 10.3390/biomedicines9091112

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Long Non-Coding RNA TRG-AS1 Promoted Proliferation and Invasion of Lung Cancer Cells Through the miR-224-5p/SMAD4 Axis

Authors: Mengyan Zhang, Weiguo Zhu, Mansour Haeryfar, Sumei Jiang, Xiang Jiang, Wei Chen, Jiancheng Li

Journal: Onco Targets Ther

PubMed ID: 34408438

Doi: 10.2147/OTT.S297336

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Ion Transport Activity Assay for Microbial Rhodopsin Expressed in Cells

Authors: Masae Konno, Keiichi Inoue, Hideki Kandori

Journal: Bio Protoc

PubMed ID: 34458409

Doi: 10.21769/BioProtoc.4115

Products & Services: Bacterial Expression

Insertion of prpoD_rpoS fragment enhances expression of recombinant protein by dps auto-inducible promoter in Escherichia coli

Authors: Dina Mulyanti, Sundani Nurono Soewandhi, Catur Riani

Journal: Mol Biol Rep

PubMed ID: 34342815

Doi: 10.1007/s11033-021-06562-z

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Interaction of Human C5a with the Major Peptide Fragments of C5aR1: Direct Evidence in Support of "Two-Site" Binding Paradigm

Authors: Aurosikha Das, Lalita Mohan Behera, Soumendra Rana

Journal: ACS Omega

PubMed ID: 34514259

Doi: 10.1021/acsomega.1c03400

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Engineering analysis of multienzyme cascade reactions for 3'-sialyllactose synthesis

Authors: Sabine Schelch, Manuel Eibinger, Stefanie Gross Belduma, Barbara Petschacher, Jürgen Kuballa, Bernd Nidetzky

Journal: Biotechnol Bioeng

PubMed ID: 34289079

Doi: 10.1002/bit.27898

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Does Differential Receptor Distribution Underlie Variable Responses to a Neuropeptide in the Lobster Cardiac System?

Authors: Audrey J Muscato, Patrick Walsh, Sovannarath Pong, Alixander Pupo, Roni J Gross, Andrew E Christie, J Joe Hull, Patsy S Dickinson

Journal: Int J Mol Sci

PubMed ID: 34445418

Doi: 10.3390/ijms22168703

Products & Services: Proteins, Expression, Isolation and Analysis

Genome-Wide Identification and Characterization of KNOTTED-Like Homeobox (KNOX) Homologs in Garlic ( L) and Their Expression Profilings Responding to Exogenous Cytokinin and Gibberellin

Authors: Siyu Zhang, Yupeng Pan, Chengchen Zhi, Yujie Zheng, Xi'ao Wang, Xiaxia Li, Zhihui Cheng

Journal: Int J Mol Sci

PubMed ID: 34502163

Doi: 10.3390/ijms22179237

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Platelet-derived growth factor (PDGF)-BB protects dopaminergic neurons via activation of Akt/ERK/CREB pathways to upregulate tyrosine hydroxylase

Authors: Huan Chen, Yan Teng, Xingmin Chen, Zhihao Liu, Fan Geng, Yanzhuo Liu, Haisong Jiang, Ziyan Wang, Lu Yang

Journal: CNS Neurosci Ther

PubMed ID: 34346167

Doi: 10.1111/cns.13708

Products & Services: Mammalian Expression

Inhibition of NOD1 Attenuates Neonatal Hypoxia-Ischemia Induced Long-Term Cognitive Impairments in Mice Through Modulation of Autophagy-Related Proteins

Authors: Fang Liu, Mingyu Shao, Feng Xu, Fang Rong

Journal: Neuropsychiatr Dis Treat

PubMed ID: 34421301

Doi: 10.2147/NDT.S314884

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Evidence for UV-green dichromacy in the basal hymenopteran Sirex noctilio (Siricidae)

Authors: Quentin Guignard, Johannes Spaethe, Bernard Slippers, Martin Strube-Bloss, Jeremy D Allison

Journal: Sci Rep

PubMed ID: 34341410

Doi: 10.1038/s41598-021-95107-2

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Objective Measurements Associated with the Preferred Eating Qualities of Fermented Salamis

Authors: Jihan Kim, Scott Knowles, Raise Ahmad, Li Day

Journal: Foods

PubMed ID: 34574113

Doi: 10.3390/foods10092003

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Inhibition of Three Citrus Pathogenic Fungi by Peptide PAF56 Involves Cell Membrane Damage

Authors: Wenjun Wang, Guirong Feng, Xindan Li, Changqing Ruan, Jian Ming, Kaifang Zeng

Journal: Foods

PubMed ID: 34574141

Doi: 10.3390/foods10092031

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CRISPR-mediated Labeling of Cells in Chick Embryos Based on Selectively Expressed Genes

Authors: Masahito Yamagata, Joshua R Sanes

Journal: Bio Protoc

PubMed ID: 34458399

Doi: 10.21769/BioProtoc.4105

Products & Services: Recombinant Proteins

Butyryl/Caproyl-CoA:Acetate CoA-transferase: cloning, expression and characterization of the key enzyme involved in medium-chain fatty acid biosynthesis

Authors: Qingzhuoma Yang, Shengtao Guo, Qi Lu, Yong Tao, Decong Zheng, Qinmao Zhou, Jun Liu

Journal: Biosci Rep

PubMed ID: 34338280

Doi: 10.1042/BSR20211135

Products & Services: Proteins, Expression, Isolation and Analysis

Hydrolysis of a second Asp-Pro site at the N-terminus of NOTCH3 in inherited vascular dementia

Authors: Xiaojie Zhang, Soo Jung Lee, Michael M Wang

Journal: Sci Rep

PubMed ID: 34446744

Doi: 10.1038/s41598-021-96679-9

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