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Production, purification and characterization of a double-tagged TEV protease

Authors: Joaquín Dalla Rizza, Claudia Ortega, Federico Carrión, Martín Fló, Agustín Correa

Journal: Protein Expr Purif

PubMed ID: 34798273

Doi: 10.1016/j.pep.2021.106021

Products & Services: Gene Synthesis

Detection of Antibodies Against the SARS-CoV-2 Spike Protein and Analysis of the Peripheral Blood Mononuclear Cell Transcriptomic Profile, 15 Years After Recovery From SARS

Authors: Lili Zhao, Na Han, Yali Zheng, Huiying Rao, Jia Li, Yanwen Chen, Bing Yu, Yu Xu, Hongsong Chen, Zhancheng Gao, Baoguo Jiang

Journal: Front Cell Infect Microbiol

PubMed ID: 34869070

Doi: 10.3389/fcimb.2021.768993

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A binary effector module secreted by a type VI secretion system

Authors: Yasmin Dar, Biswanath Jana, Eran Bosis, Dor Salomon

Journal: EMBO Rep

PubMed ID: 34752000

Doi: 10.15252/embr.202153981

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High salt exacerbates acute kidney injury by disturbing the activation of CD5L/apoptosis inhibitor of macrophage (AIM) protein

Authors: Ching-Ting Wang, Tetsushi Tezuka, Naoki Takeda, Kimi Araki, Satoko Arai, Toru Miyazaki

Journal: PLoS ONE

PubMed ID: 34843572

Doi: 10.1371/journal.pone.0260449

Products & Services: Proteins, Expression, Isolation and Analysis

Cryo-EM snapshots of a native lysate provide structural insights into a metabolon-embedded transacetylase reaction

Authors: Christian Tüting, Fotis L Kyrilis, Johannes Müller, Marija Sorokina, Ioannis Skalidis, Farzad Hamdi, Yashar Sadian, Panagiotis L Kastritis

Journal: Nat Commun

PubMed ID: 34836937

Doi: 10.1038/s41467-021-27287-4

Products & Services: Proteins, Expression, Isolation and Analysis

A Novel Serum tsRNA for Diagnosis and Prediction of Nephritis in SLE

Authors: Ping Yang, Xiaoshan Zhang, Shanshan Chen, Yue Tao, Mingzhe Ning, Yijia Zhu, Jun Liang, Wei Kong, Bo Shi, Zhiyang Li, Han Shen, Yanbo Wang

Journal: Front Immunol

PubMed ID: 34867955

Doi: 10.3389/fimmu.2021.735105

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A novel chimeric protein with enhanced cytotoxic effects on breast cancer in vitro and in vivo

Authors: Fereshte Hazrati, Massoud Saidijam, Yaghoub Ahmadyousefi, Fatemeh Nouri, Hamidreza Ghadimipour, Mohammadreza Moradi, Rasool Haddadi, Meysam Soleimani

Journal: Proteins

PubMed ID: 34812523

Doi: 10.1002/prot.26285

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Diagnostic Performance of Self-Collected Saliva Versus Nasopharyngeal Swab for the Molecular Detection of SARS-CoV-2 in the Clinical Setting

Authors: Mohammad Khaja Mafij Uddin, Tahmina Shirin, Mohammad Enayet Hossain, Ahmed Nawsher Alam, Jenifar Quaiyum Ami, Rashedul Hasan, Mojnu Miah, Nusrat Jahan Shaly, Shahriar Ahmed, S M Mazidur Rahman, Mustafizur Rahman, Sayera Banu

Journal: Microbiol Spectr

PubMed ID: 34730436

Doi: 10.1128/Spectrum.00468-21

Products & Services: Gene Synthesis

Identification and evolutionary analysis of the metal-tolerance protein family in eight Cucurbitaceae species

Authors: Xinchen Jiang, Junliang Yin, Lixin Wang, Keyong Xi, Xiongmeng Zhu, Gang Li, Yongxing Zhu, Yiqing Liu

Journal: Plant Genome

PubMed ID: 34741493

Doi: 10.1002/tpg2.20167

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Cofactors and pathogens: Flavin mononucleotide and flavin adenine dinucleotide (FAD) biosynthesis by the FAD synthase from Brucella ovis

Authors: Andrea Moreno, Victor Taleb, María Sebastián, Ernesto Anoz-Carbonell, Marta Martínez-Júlvez, Milagros Medina

Journal: IUBMB Life

PubMed ID: 34813144

Doi: 10.1002/iub.2576

Products & Services: Gene Synthesis

Increase in Ischemia-Modified Albumin and Pregnancy-Associated Plasma Protein-A in COVID-19 Patients

Authors: Belén G Sanchez, Jose M Gasalla, Manuel Sánchez-Chapado, Alicia Bort, Inés Diaz-Laviada

Journal: J Clin Med

PubMed ID: 34884175

Doi: 10.3390/jcm10235474

Products & Services: Mammalian Expression

Antibody Generation and Immunogenicity Analysis of EBV gp42 N-Terminal Region

Authors: Junping Hong, Dongmei Wei, Qian Wu, Ling Zhong, Kaiyun Chen, Yang Huang, Wanlin Zhang, Junyu Chen, Ningshao Xia, Xiao Zhang, Yixin Chen

Journal: Viruses

PubMed ID: 34960650

Doi: 10.3390/v13122380

Products & Services: Proteins, Expression, Isolation and Analysis

The CIP2A-TOPBP1 axis safeguards chromosome stability and is a synthetic lethal target for BRCA-mutated cancer

Authors: Salomé Adam, Silvia Emma Rossi, Nathalie Moatti, Mara De Marco Zompit, Yibo Xue, Timothy F Ng, Alejandro Álvarez-Quilón, Jessica Desjardins, Vivek Bhaskaran, Giovanni Martino, Dheva Setiaputra, Sylvie M Noordermeer, Toshiro K Ohsumi, Nicole Hustedt, Rachel K Szilard, Natasha Chaudhary, Meagan Munro, Artur Veloso, Henrique Melo, Shou Yun Yin, Robert Papp, Jordan T F Young, Michael Zinda, Manuel Stucki, Daniel Durocher

Journal: Nat Cancer

PubMed ID: 35121901

Doi: 10.1038/s43018-021-00266-w

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Lipopolysaccharide O-antigen molecular and supramolecular modifications of plant root microbiota are pivotal for host recognition

Authors: Adele Vanacore, Giuseppe Vitiello, Alan Wanke, Domenico Cavasso, Luke A Clifton, Lisa Mahdi, María Asunción Campanero-Rhodes, Dolores Solís, Manfred Wuhrer, Simone Nicolardi, Antonio Molinaro, Roberta Marchetti, Alga Zuccaro, Luigi Paduano, Alba Silipo

Journal: Carbohydr Polym

PubMed ID: 34893256

Doi: 10.1016/j.carbpol.2021.118839

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Assessment of in vitro activities of novel modified antimicrobial peptides against clarithromycin resistant Mycobacterium abscessus

Authors: Phantitra Sudadech, Sittiruk Roytrakul, Orawee Kaewprasert, Auttawit Sirichoat, Ploenchan Chetchotisakd, Sakawrat Kanthawong, Kiatichai Faksri

Journal: PLoS ONE

PubMed ID: 34780520

Doi: 10.1371/journal.pone.0260003

Products & Services: Peptide Synthesis

A molecular dynamics-guided mutagenesis identifies two aspartic acid residues involved in the pH-dependent activity of OG-OXIDASE 1

Authors: Anna Scortica, Matteo Capone, Daniele Narzi, Mario Frezzini, Valentina Scafati, Moira Giovannoni, Francesco Angelucci, Leonardo Guidoni, Benedetta Mattei, Manuel Benedetti

Journal: Plant Physiol Biochem

PubMed ID: 34800821

Doi: 10.1016/j.plaphy.2021.11.011

Products & Services: Gene Synthesis

A super PPR cluster for restoring fertility revealed by genetic mapping, homocap-seq and de novo assembly in cotton

Authors: Bin Gao, Gaofeng Ren, Tianwang Wen, Haiping Li, Xianlong Zhang, Zhongxu Lin

Journal: Theor Appl Genet

PubMed ID: 34811574

Doi: 10.1007/s00122-021-03990-0

Products & Services: Protein Tools

The σ1 Receptor and the HINT1 Protein Control α2δ1 Binding to Glutamate NMDA Receptors: Implications in Neuropathic Pain

Authors: María Rodríguez-Muñoz, Elsa Cortés-Montero, Yara Onetti, Pilar Sánchez-Blázquez, Javier Garzón-Niño

Journal: Biomolecules

PubMed ID: 34827679

Doi: 10.3390/biom11111681

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The Q61H mutation decouples KRAS from upstream regulation and renders cancer cells resistant to SHP2 inhibitors

Authors: Teklab Gebregiworgis, Yoshihito Kano, Jonathan St-Germain, Nikolina Radulovich, Molly L Udaskin, Ahmet Mentes, Richard Huang, Betty P K Poon, Wenguang He, Ivette Valencia-Sama, Claire M Robinson, Melissa Huestis, Jinmin Miao, Jen Jen Yeh, Zhong-Yin Zhang, Meredith S Irwin, Jeffrey E Lee, Ming-Sound Tsao, Brian Raught, Christopher B Marshall, Michael Ohh, Mitsuhiko Ikura

Journal: Nat Commun

PubMed ID: 34725361

Doi: 10.1038/s41467-021-26526-y

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Tasquinimod efficacy and S100A9 expression in glucose-treated HREC cells

Authors: Ji Jin, Ji Zhang, Shuyang Bu

Journal: Int Ophthalmol

PubMed ID: 34796432

Doi: 10.1007/s10792-021-02038-y

Products & Services: Gene Synthesis

Debulking SARS-CoV-2 in saliva using angiotensin converting enzyme 2 in chewing gum to decrease oral virus transmission and infection

Authors: Henry Daniell, Smruti K Nair, Nardana Esmaeili, Geetanjali Wakade, Naila Shahid, Prem Kumar Ganesan, Md Reyazul Islam, Ariel Shepley-McTaggart, Sheng Feng, Ebony N Gary, Ali R Ali, Manunya Nuth, Selene Nunez Cruz, Jevon Graham-Wooten, Stephen J Streatfield, Ruben Montoya-Lopez, Paul Kaznica, Margaret Mawson, Brian J Green, Robert Ricciardi, Michael Milone, Ronald N Harty, Ping Wang, David B Weiner, Kenneth B Margulies, Ronald G Collman

Journal: Mol Ther

PubMed ID: 34774754

Doi: 10.1016/j.ymthe.2021.11.008

Products & Services: Plasmid DNA Preparation

The myocardial infarction-associated transcript 2 inhibits lipid accumulation and promotes cholesterol efflux in oxidized low-density lipoprotein-induced THP-1-derived macrophages via inhibiting mitogen-activated protein kinase signaling and activating the nuclear factor erythroid-related factor 2 signaling pathway

Authors: Fangxiang Mu, Yuqing Wang, Hong Wu, Qingxia You, Daimin Zhang

Journal: Bioengineered

PubMed ID: 34783297

Doi: 10.1080/21655979.2021.2005932

Products & Services: Plasmid DNA Preparation

Inhibition of the hERG potassium channel by phenanthrene: a polycyclic aromatic hydrocarbon pollutant

Authors: Ehab Al-Moubarak, Holly A Shiels, Yihong Zhang, Chunyun Du, Oliver Hanington, Stephen C Harmer, Christopher E Dempsey, Jules C Hancox

Journal: Cell Mol Life Sci

PubMed ID: 34727194

Doi: 10.1007/s00018-021-03967-8

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Formation of nuclear condensates by the Mediator complex subunit Med15 in mammalian cells

Authors: Yuanyuan Shi, Jian Chen, Wei-Jie Zeng, Miao Li, Wenxue Zhao, Xing-Ding Zhang, Jie Yao

Journal: BMC Biol

PubMed ID: 34789250

Doi: 10.1186/s12915-021-01178-y

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DeepPhospho accelerates DIA phosphoproteome profiling through in silico library generation

Authors: Ronghui Lou, Weizhen Liu, Rongjie Li, Shanshan Li, Xuming He, Wenqing Shui

Journal: Nat Commun

PubMed ID: 34795227

Doi: 10.1038/s41467-021-26979-1

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Long Non-Coding RNA Regulates Neuronal Differentiation of Neural Stem Cells During Brain Development

Authors: Elpinickie Ninou, Artemis Michail, Panagiotis K Politis

Journal: Front Cell Dev Biol

PubMed ID: 34900989

Doi: 10.3389/fcell.2021.726857

Products & Services: GenCrispr Cas9 Genome Editing

Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos

Authors: Raghuvir Viswanatha, Enzo Mameli, Jonathan Rodiger, Pierre Merckaert, Fabiana Feitosa-Suntheimer, Tonya M Colpitts, Stephanie E Mohr, Yanhui Hu, Norbert Perrimon

Journal: Nat Commun

PubMed ID: 34819517

Doi: 10.1038/s41467-021-27129-3

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An Efficient Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-Associated Protein 9 Mutagenesis System for Oil Palm ()

Authors: Wan-Chin Yeap, Muhammad Rashdan Muad, David Ross Appleton

Journal: Front Plant Sci

PubMed ID: 34880893

Doi: 10.3389/fpls.2021.773656

Products & Services: Gene Synthesis

SARS-CoV-2 Spike Protein and Its Receptor Binding Domain Promote a Proinflammatory Activation Profile on Human Dendritic Cells

Authors: Dante Barreda, César Santiago, Juan R Rodríguez, José F Rodríguez, José M Casasnovas, Isabel Mérida, Antonia Ávila-Flores

Journal: Cells

PubMed ID: 34943787

Doi: 10.3390/cells10123279

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DNA Matrix Operation Based on the Mechanism of the DNAzyme Binding to Auxiliary Strands to Cleave the Substrate

Authors: Shaoxia Xu, Yuan Liu, Shihua Zhou, Qiang Zhang, Nikola K Kasabov

Journal: Biomolecules

PubMed ID: 34944442

Doi: 10.3390/biom11121797

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Mast Cells and Basophils in the Defense against Ectoparasites: Efficient Degradation of Parasite Anticoagulants by the Connective Tissue Mast Cell Chymases

Authors: Zhirong Fu, Srinivas Akula, Anna-Karin Olsson, Jukka Kervinen, Lars Hellman

Journal: Int J Mol Sci

PubMed ID: 34884431

Doi: 10.3390/ijms222312627

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Nanovesicles derived from bispecific CAR-T cells targeting the spike protein of SARS-CoV-2 for treating COVID-19

Authors: Tianchuan Zhu, Yuchen Xiao, Xiaojun Meng, Lantian Tang, Bin Li, Zhaoyan Zhao, Qingqin Tan, Hong Shan, Lei Liu, Xi Huang

Journal: J Nanobiotechnology

PubMed ID: 34823562

Doi: 10.1186/s12951-021-01148-0

Products & Services: Proteins, Expression, Isolation and Analysis

The Excretory/Secretory and Surface Proteome Contains Putative Modulators of the Host Coagulation

Authors: Nina Gillis-Germitsch, Tobias Kockmann, Lars M Asmis, Lucienne Tritten, Manuela Schnyder

Journal: Front Cell Infect Microbiol

PubMed ID: 34796127

Doi: 10.3389/fcimb.2021.753320

Products & Services: Proteins, Expression, Isolation and Analysis

Epitope-coated polymer particles elicit neutralising antibodies against Plasmodium falciparum sporozoites

Authors: Benjamin J Evert, Shuxiong Chen, Robyn McConville, Ryan W J Steel, Julie Healer, Justin A Boddey, Lucas Huntimer, Bernd H A Rehm

Journal: npj Vaccines

PubMed ID: 34845267

Doi: 10.1038/s41541-021-00408-2

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Proliferation of Tau 304-380 Fragment Aggregates through Autocatalytic Secondary Nucleation

Authors: Diana C Rodriguez Camargo, Eimantas Sileikis, Sean Chia, Emil Axell, Katja Bernfur, Rodrigo L Cataldi, Samuel I A Cohen, Georg Meisl, Johnny Habchi, Tuomas P J Knowles, Michele Vendruscolo, Sara Linse

Journal: ACS Chem Neurosci

PubMed ID: 34783519

Doi: 10.1021/acschemneuro.1c00454

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High-throughput and high-dimensional single-cell analysis of antigen-specific CD8 T cells

Authors: Ke-Yue Ma, Alexandra A Schonnesen, Chenfeng He, Amanda Y Xia, Eric Sun, Eunise Chen, Katherine R Sebastian, Yu-Wan Guo, Robert Balderas, Mrinalini Kulkarni-Date, Ning Jiang

Journal: Nat Immunol

PubMed ID: 34811538

Doi: 10.1038/s41590-021-01073-2

Products & Services: Proteins, Expression, Isolation and Analysis

Assessment of an LSDV-Vectored Vaccine for Heterologous Prime-Boost Immunizations against HIV

Authors: Ros Chapman, Michiel van Diepen, Nicola Douglass, Shireen Galant, Mohamed Jaffer, Emmanuel Margolin, Phindile Ximba, Tandile Hermanus, Penny L Moore, Anna-Lise Williamson

Journal: Vaccines (Basel)

PubMed ID: 34835214

Doi: 10.3390/vaccines9111281

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Patterns of Tetracapsuloides bryosalmonae infection of three salmonid species in large, deep Norwegian lakes

Authors: Tone Jøran Oredalen, Mona Saebø, Tor Atle Mo

Journal: J Fish Dis

PubMed ID: 34747501

Doi: 10.1111/jfd.13548

Products & Services: Plasmid DNA Preparation

Scaling DNA data storage with nanoscale electrode wells

Authors: Bichlien H Nguyen, Christopher N Takahashi, Gagan Gupta, Jake A Smith, Richard Rouse, Paul Berndt, Sergey Yekhanin, David P Ward, Siena D Ang, Patrick Garvan, Hsing-Yeh Parker, Rob Carlson, Douglas Carmean, Luis Ceze, Karin Strauss

Journal: Sci Adv

PubMed ID: 34818035

Doi: 10.1126/sciadv.abi6714

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A feminizing switch in a hemimetabolous insect

Authors: Ji-Chong Zhuo , Hou-Hong Zhang , Qing-Ling Hu , Jin-Li Zhang , Jia-Bao Lu , Han-Jing Li , Yu-Cheng Xie , Wei-Wei Wang , Yan Zhang , Hai-Qiang Wang , Hai-Jian Huang , Gang Lu , Jian-Ping Chen , Jun-Min Li , Zhi-Jian Tu , Chuan-Xi Zhang

Journal: Sci Adv

PubMed ID: 34826246

Doi: 10.1126/sciadv.abf9237

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A novel effector, CsSp1, from Bipolaris sorokiniana, is essential for colonization in wheat and is also involved in triggering host immunity

Authors: Wanying Zhang, Haiyang Li, Limin Wang, Shunpei Xie, Yuan Zhang, Ruijiao Kang, Mengjuan Zhang, Panpan Zhang, Yonghui Li, Yanfeng Hu, Min Wang, Linlin Chen, Hongxia Yuan, Shengli Ding, Honglian Li

Journal: Mol Plant Pathol

PubMed ID: 34741560

Doi: 10.1111/mpp.13155

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Recognition of Variants of Concern by Antibodies and T Cells Induced by a SARS-CoV-2 Inactivated Vaccine

Authors: Felipe Melo-González, Jorge A Soto, Liliana A González, Jorge Fernández, Luisa F Duarte, Bárbara M Schultz, Nicolás M S Gálvez, Gaspar A Pacheco, Mariana Ríos, Yaneisi Vázquez, Daniela Rivera-Pérez, Daniela Moreno-Tapia, Carolina Iturriaga, Omar P Vallejos, Roslye V Berríos-Rojas, Guillermo Hoppe-Elsholz, Marcela Urzúa, Nicole Bruneau, Rodrigo A Fasce, Judith Mora, Alba Grifoni, Alessandro Sette, Daniela Weiskopf, Gang Zeng, Weining Meng, José V González-Aramundiz, Pablo A González, Katia Abarca, Eugenio Ramírez, Alexis M Kalergis, Susan M Bueno

Journal: Front Immunol

PubMed ID: 34858404

Doi: 10.3389/fimmu.2021.747830

Products & Services: Recombinant Antibody Expression

Alternative Methods to Detect Severe Acute Respiratory Syndrome Coronavirus 2 Antibodies

Authors: Rashmi Patel, Siddharth Khare, Vinay S Mahajan

Journal: Clin Lab Med

PubMed ID: 35153048

Doi: 10.1016/j.cll.2021.10.007

A Mycobacteriophage-Based Vaccine Platform: SARS-CoV-2 Antigen Expression and Display

Authors: Krista G Freeman, Katherine S Wetzel, Yu Zhang, Kira M Zack, Deborah Jacobs-Sera, Sara M Walters, Dominique J Barbeau, Anita K McElroy, John V Williams, Graham F Hatfull

Journal: Microorganisms

PubMed ID: 34946016

Doi: 10.3390/microorganisms9122414

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Reprogramming NK cells and macrophages via combined antibody and cytokine therapy primes tumors for elimination by checkpoint blockade

Authors: Chensu Wang, Ang Cui, Maurice Bukenya, Aereas Aung, Dikshant Pradhan, Charles A Whittaker, Yash Agarwal, Ayush Thomas, Simon Liang, Parastoo Amlashi, Heikyung Suh, Stefani Spranger, Nir Hacohen, Darrell J Irvine

Journal: Cell Rep

PubMed ID: 34818534

Doi: 10.1016/j.celrep.2021.110021

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Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling

Authors: In-Chi Young, Bogang Wu, Jaclyn Andricovich, Sung-Ting Chuang, Rong Li, Alexandros Tzatsos, Ray-Chang Wu, Mei-Yi Wu

Journal: Cell Rep

PubMed ID: 34818550

Doi: 10.1016/j.celrep.2021.110036

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Nanoparticular CpG-adjuvanted SARS-CoV-2 S1 protein elicits broadly neutralizing and Th1-biased immunoreactivity in mice

Authors: Hui-Tsu Lin, Cheng-Cheung Chen, Der-Jiang Chiao, Tein-Yao Chang, Xin-An Chen, Jenn-Jong Young, Szu-Cheng Kuo

Journal: Int J Biol Macromol

PubMed ID: 34774590

Doi: 10.1016/j.ijbiomac.2021.11.020

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Nanovaccines Mediated Subcutis-to-Intestine Cascade for Improved Protection against Intestinal Infections

Authors: Xiaofang Zhong, Guangsheng Du, Xuanyu Wang, Yangsen Ou, Hairui Wang, Yining Zhu, Xinyan Hao, Zhiqiang Xie, Yuandong Zhang, Tao Gong, Zhirong Zhang, Xun Sun

Journal: Small

PubMed ID: 34825482

Doi: 10.1002/smll.202105530

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Baculovirus Vectors Induce the Production of Interferons in Swine: Their Potential in the Development of Antiviral Strategies

Authors: Guido Nicolás Molina, Sabrina Amalfi, Ignacio Otero, Oscar Taboga, María Paula Molinari

Journal: Vet Sci

PubMed ID: 34822651

Doi: 10.3390/vetsci8110278

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Metallothionein 3-Zinc Axis Suppresses Caspase-11 Inflammasome Activation and Impairs Antibacterial Immunity

Authors: Debabrata Chowdhury, Jason C Gardner, Abhijit Satpati, Suba Nookala, Santhosh Mukundan, Aleksey Porollo, Julio A Landero Figueroa, Kavitha Subramanian Vignesh

Journal: Front Immunol

PubMed ID: 34867993

Doi: 10.3389/fimmu.2021.755961

Products & Services: Proteins, Expression, Isolation and Analysis

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