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A replicon RNA vaccine induces durable protective immunity from SARS-CoV-2 in nonhuman primates after neutralizing antibodies have waned

Authors: Megan A Oâ Connor, David W Hawman, Kimberly Meade-White, Shanna Leventhal, Wenjun Song, Samantha Randall, Jacob Archer, Thomas B Lewis, Brieann Brown, Naoto Iwayama, Chul Ahrens, William Garrison, Solomon Wangari, Kathryn A Guerriero, Patrick Hanley, Jamie Lovaglio, Greg Saturday, Paul T Edlefsen, Amit Khandhar, Heinz Feldmann, Deborah Heydenburg Fuller, Jesse H Erasmus

Journal: biorxiv

PubMed ID: 35982677

Doi: 10.1101/2022.08.08.503239

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A novel method to purify small RNAs from human tissues for methylation analysis by LC-MS/MS

Authors: Rong Yang, Jianfeng Li, Yifan Wu, Xinli Jiang, Shuang Qu, Qiang Wang, Hongwei Liang, Ke Zen

Journal: Front Mol Biosci

PubMed ID: 36111135

Doi: 10.3389/fmolb.2022.949181

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α-Synuclein Interaction with Lipid Bilayer Discs

Authors: Marija Dubackic, Yun Liu, Elizabeth G Kelley, Crispin Hetherington, Michael Haertlein, Juliette M Devos, Sara Linse, Emma Sparr, Ulf Olsson

Journal: Langmuir

PubMed ID: 35952001

Doi: 10.1021/acs.langmuir.2c01368

Products & Services: Bacterial Expression

Characterization of Treponema denticola Major Surface Protein (Msp) by Deletion Analysis and Advanced Molecular Modeling

Authors: M Paula Goetting-Minesky, Valentina Godovikova, Wei Zheng, J Christopher Fenno

Journal: J Bacteriol

PubMed ID: 35913147

Doi: 10.1128/jb.00228-22

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Biophysical characterization of light-gated ion channels using planar automated patch clamp

Authors: Elena G Govorunova, Oleg A Sineshchekov, Leonid S Brown, John L Spudich

Journal: Front Mol Neurosci

PubMed ID: 36017077

Doi: 10.3389/fnmol.2022.976910

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The key molecular pattern BxCDP1 of induces plant immunity and enhances plant defense response two small peptide regions

Authors: Long-Jiao Hu, Xiao-Qin Wu, Tong-Yue Wen, Jian-Ren Ye, Yi-Jun Qiu, Lin Rui, Yan Zhang

Journal: Front Plant Sci

PubMed ID: 35991456

Doi: 10.3389/fpls.2022.937473

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A Defect in Lipoprotein Modification by Lgt Leads to Abnormal Morphology and Cell Death in Escherichia coli That Is Independent of Major Lipoprotein Lpp

Authors: S Legood, D Seng, I G Boneca, N Buddelmeijer

Journal: J Bacteriol

PubMed ID: 35938851

Doi: 10.1128/jb.00164-22

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The Use of Reproductive Indicators for Conservation Purposes: The Case Study of in Two Fully Protected Areas and Their Surrounding Zones (Central-Western Mediterranean)

Authors: Cristina Porcu, Laura Carugati, Andrea Bellodi, Pierluigi Carbonara, Alessandro Cau, Danila Cuccu, Faustina Barbara Cannea, Martina Francesca Marongiu, Antonello Mulas, Alessandra Padiglia, Noemi Pascale, Paola Pesci, Maria Cristina Follesa

Journal: Biology (Basel)

PubMed ID: 36009815

Doi: 10.3390/biology11081188

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HGT is widespread in insects and contributes to male courtship in lepidopterans

Authors: Yang Li , Zhiguo Liu , Chao Liu , Zheyi Shi , Lan Pang , Chuzhen Chen , Yun Chen , Ronghui Pan , Wenwu Zhou , Xue-Xin Chen , Antonis Rokas , Jianhua Huang , Xing-Xing Shen

Journal: Cell

PubMed ID: 35853453

Doi: doi.org/10.1016/j.cell.2022.06.014

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Regulation of Host Immune Response against Proteins via Computational mRNA Vaccine Design through Transcriptional Modification

Authors: Muhammad Naveed, Khizra Jabeen, Rubina Naz, Muhammad Saad Mughal, Ali A Rabaan, Muhammed A Bakhrebah, Fahad M Alhoshani, Mohammed Aljeldah, Basim R Al Shammari, Mohammed Alissa, Amal A Sabour, Rana A Alaeq, Maha A Alshiekheid, Mohammed Garout, Mohammed S Almogbel, Muhammad A Halwani, Safaa A Turkistani, Naveed Ahmed

Journal: Microorganisms

PubMed ID: 36014038

Doi: 10.3390/microorganisms10081621

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Individualized, heterologous chimpanzee adenovirus and self-amplifying mRNA neoantigen vaccine for advanced metastatic solid tumors: phase 1 trial interim results

Authors: Christine D Palmer, Amy R Rappaport, Matthew J Davis, Meghan G Hart, Ciaran D Scallan, Sue-Jean Hong, Leonid Gitlin, Lauren D Kraemer, Sonia Kounlavouth, Aaron Yang, Lindsey Smith, Desiree Schenk, Mojca Skoberne, Kiara Taquechel, Martina Marrali, Jason R Jaroslavsky, Charmaine N Nganje, Elizabeth Maloney, Rita Zhou, Daniel Navarro-Gomez, Adrienne C Greene, Gijsbert Grotenbreg, Renee Greer, Wade Blair, Minh Duc Cao, Shawn Chan, Kyounghwa Bae, Alexander I Spira, Sameek Roychowdhury, David P Carbone, Brian S Henick, Charles G Drake, Benjamin J Solomon, Daniel H Ahn, Amit Mahipal, Steve B Maron, Benny Johnson, Raphael Rousseau, Roman Yelensky, Chih-Yi Liao, Daniel V T Catenacci, Andrew Allen, Andrew R Ferguson, Karin Jooss

Journal: Nat Med

PubMed ID: 35970920

Doi: 10.1038/s41591-022-01937-6

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Ancestral Sequence Reconstruction Identifies Structural Changes Underlying the Evolution of PETase and Variants with Improved Stability and Activity

Authors: Yvonne Joho, Vanessa Vongsouthi, Matthew A Spence, Jennifer Ton, Chloe Gomez, Li Lynn Tan, Joe A Kaczmarski, Alessandro T Caputo, Santana Royan, Colin J Jackson, Albert Ardevol

Journal: Biochemistry

PubMed ID: 35951410

Doi: 10.1021/acs.biochem.2c00323

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Rabbit hemorrhagic disease virus VP60 protein expressed in recombinant swinepox virus self-assembles into virus-like particles with strong immunogenicity in rabbits

Authors: Changjin Liu, Min Lin, Huanyi Hu, Xiaolan Liu, Yanchao Bian, Xiaohua Huang, Xiaoxiang Li, Wenyang Yu, Feng Luo, Shunzhou Deng

Journal: Front Microbiol

PubMed ID: 35992711

Doi: 10.3389/fmicb.2022.960374

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Characterization of interaction between blood coagulation factor VIII and LRP1 suggests dynamic binding by alternating complex contacts

Authors: Haarin Chun, James H Kurasawa, Philip Olivares, Ekaterina S Marakasova, Svetlana A Shestopal, Gabriela U Hassink, Elena Karnaukhova, Mary Migliorini, Juliet O Obi, Ally K Smith, Patrick L Wintrode, Prasannavenkatesh Durai, Keunwan Park, Daniel Deredge, Dudley K Strickland, Andrey G Sarafanov

Journal: J Thromb Haemost

PubMed ID: 35810466

Doi: 10.1111/jth.15817

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HIV-1-Infected CD4 T Cells Present MHC Class II-Restricted Epitope via Endogenous Processing

Authors: Mary M Addison, Gavin I Ellis, George J Leslie, Noah B Zawadzky, James L Riley, James A Hoxie, Laurence C Eisenlohr

Journal: J Immunol

PubMed ID: 36130133

Doi: 10.4049/jimmunol.2200145

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Streptococcal autolysin promotes dysfunction of swine tracheal epithelium by interacting with vimentin

Authors: Yu Meng, Qing Wang, Zhe Ma, Weiyi Li, Kai Niu, Ting Zhu, Huixing Lin, Chengping Lu, Hongjie Fan

Journal: PLoS Pathog

PubMed ID: 35921364

Doi: 10.1371/journal.ppat.1010765

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Host and viral determinants of airborne transmission of SARS-CoV-2 in the Syrian hamster

Authors: Julia R Port, Dylan H Morris, Jade C Riopelle, Claude Kwe Yinda, Victoria A Avanzato, Myndi G Holbrook, Trenton Bushmaker, Jonathan E Schulz, Taylor A Saturday, Kent Barbian, Colin A Russell, Rose Perry-Gottschalk, Carl I Shaia, Craig Martens, James O Lloyd-Smith, Robert J Fischer, Vincent J Munster

Journal: biorxiv

PubMed ID: 36032963

Doi: 10.1101/2022.08.15.504010

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Resolving the zinc binding capacity of honey bee vitellogenin and locating its putative binding sites

Authors: Vilde Leipart, Øyvind Enger, Diana Cornelia Turcu, Olena Dobrovolska, Finn Drabløs, Øyvind Halskau, Gro V Amdam

Journal: Insect Mol Biol

PubMed ID: 36054587

Doi: 10.1111/imb.12807

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Dysregulated naive B cells and de novo autoreactivity in severe COVID-19

Authors: Matthew C Woodruff, Richard P Ramonell, Natalie S Haddad, Fabliha A Anam, Mark E Rudolph, Tiffany A Walker, Alexander D Truong, Adviteeya N Dixit, Jenny E Han, Monica Cabrera-Mora, Martin C Runnstrom, Regina Bugrovsky, Jennifer Hom, Erin C Connolly, Igor Albizua, Vidhi Javia, Kevin S Cashman, Doan C Nguyen, Shuya Kyu, Ankur Singh Saini, Michael Piazza, Christopher M Tipton, Arezou Khosroshahi, Greg Gibson, Greg S Martin, Cheryl L Maier, Annette Esper, Scott A Jenks, F Eun-Hyung Lee, Ignacio Sanz

Journal: Nature

PubMed ID: 36044993

Doi: 10.1038/s41586-022-05273-0

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Riboflavin (Vitamin B2) Deficiency Induces Apoptosis Mediated by Endoplasmic Reticulum Stress and the CHOP Pathway in HepG2 Cells

Authors: Bo Zhang, Jun-Ting Cao, Yong-Bao Wu, Ke-Xin Gao, Ming Xie, Zheng-Kui Zhou, Jing Tang, Shui-Sheng Hou

Journal: Nutrients

PubMed ID: 36014863

Doi: 10.3390/nu14163356

Products & Services: Protein Electrophoresis and Western

Inhibitory effect of morin on aldolase 2 from Eimeria tenella

Authors: Junjing Hu, Mingfei Sun, Nanshan Qi, Asmaa M I Abuzeid, Juan Li, Haiming Cai, Minna Lv, Xuhui Lin, Shenquan Liao, Guoqing Li

Journal: Int J Parasitol Drugs Drug Resist

PubMed ID: 35952522

Doi: 10.1016/j.ijpddr.2022.07.002

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Rapid detection of Phytophthora cinnamomi based on a new target gene Pcinn13739

Authors: Zhenpeng Chen, Binbin Jiao, Jing Zhou, Haibin He, Tingting Dai

Journal: Frontiers in cellular and infection microbiology

Doi: 10.3389/fcimb.2022.923700

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Investigation of Transcript Variant 6 of as a Prognostic and Predictive Biomarker in Basal-Like MDA-MB-231 and MDA-MB-453 Cell Lines for Breast Cancer

Authors: Xin Zhang, Daniel O'Brien, Xiaohui Zhang

Journal: Oxid Med Cell Longev

PubMed ID: 36071863

Doi: 10.1155/2022/7078787

Products & Services: Plasmid DNA Preparation

Expedient Synthesis of Ubiquitin-like Protein ISG15 Tools Through Chemo-Enzymatic Ligation Catalyzed by a Viral Protease Lbpro

Authors: Tian Wang , Chuntong Li , Meijing Wang , Jiachen Zhang , Qingyun Zheng , Lujun Liang , Guochao Chu , Xiaolin Tian , Haiteng Deng , Wei He , Lei Liu , Jinghong Li

Journal: Angew Chem Int Ed Engl

PubMed ID: 35962463

Doi: 10.1002/anie.202206205

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Enhancing the Catalytic Activity of Type II L-Asparaginase from Bacillus licheniformis through Semi-Rational Design

Authors: Yawen Zhou ,Linshu Jiao ,Juan Shen ,Huibing Chi ,Zhaoxin Lu ,Huawei Liu ,Fengxia Lu ,Ping Zhu

Journal: Int J Mol Sci

Doi: 10.3390/ijms23179663

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Dry powder inhalation technology for heart targeting applied to calcium phosphate nanoparticles loaded with active substances

Authors: Eride Quarta,Lorenzo Degli Esposti, Ruggero Bettini ,Fabio Sonvico ,Daniele Catalucci , Michele Iafisco ,Claudio De Luca ,Giovanna Trevisi ,Paolo Colombo ,Alessandra Rossi ,Francesca Buttini , Gaia Colombo

Journal: Drug Delivery Science and Technology

Doi: doi.org/10.1016/j.jddst.2022.103719

Products & Services: Peptide Synthesis

Receptor deorphanization in an echinoderm reveals kisspeptin evolution and relationship with SALMFamide neuropeptides

Authors: Nayeli Escudero Castelán , Dean C Semmens , Luis Alfonso Yañez Guerra , Meet Zandawala , Mario Dos Reis , Susan E Slade , James H Scrivens , Cleidiane G Zampronio , Alexandra M Jones , Olivier Mirabeau , Maurice R Elphick

Journal: BMC Biol

PubMed ID: 36002813

Doi: 10.1186/s12915-022-01387-z

Products & Services: Gene Synthesis

The neurodevelopmental gene MSANTD2 belongs to a gene family formed by recurrent molecular domestication of Harbinger transposons at the base of vertebrates

Authors: Ema Etchegaray, Dominique Baas, Magali Naville, Zofia Haftek-Terreau, Jean Nicolas Volff

Journal: Mol Biol Evol

PubMed ID: 35980103

Doi: 10.1093/molbev/msac173

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Human Claudin-Derived Peptides Block the Membrane Fusion Process of Zika Virus and Are Broad Flavivirus Inhibitors

Authors: Jim Zoladek, Julien Burlaud-Gaillard, Maxime Chazal, Sophie Desgraupes, Patricia Jeannin, Antoine Gessain, Nathalie Pardigon, Mathieu Hubert, Philippe Roingeard, Nolwenn Jouvenet, Philippe V Afonso

Journal: Microbiol Spectr

PubMed ID: 36040168

Doi: 10.1128/spectrum.02989-22

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TRPV1 drugs alter core body temperature via central projections of primary afferent sensory neurons

Authors: Wendy Wing Sze Yue, Lin Yuan, Joao M Braz, Allan I Basbaum, David Julius

Journal: Elife

PubMed ID: 35968676

Doi: 10.7554/eLife.80139

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Secretion of collagenases by Saccharomyces cerevisiae for collagen degradation

Authors: Han Xiao, Xiufang Liu, Yunzi Feng, Lin Zheng, Mouming Zhao, Mingtao Huang

Journal: Biotechnol Biofuels Bioprod

PubMed ID: 36031598

Doi: 10.1186/s13068-022-02186-y

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Preferential binding of DAP-PGs by major peptidoglycan recognition proteins found in cell-free hemolymph of Manduca sexta

Authors: Udeshika Kariyawasam, Mansi Gulati, Yang Wang, Haibo Bao, Tisheng Shan, Xiuru Li, Xiaolong Cao, Niranji Sumathipala, Yingxia Hu, Xiufeng Zhang, Geert-Jan Boons, Haobo Jiang

Journal: Insect Biochem Mol Biol

PubMed ID: 36007680

Doi: 10.1016/j.ibmb.2022.103827

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Development and application of a rapid all-in-one plasmid CRISPR-Cas9 system for iterative genome editing in Bacillus subtilis

Authors: Yu Zou, Lu Qiu, Aowen Xie, Wenyuan Han, Shangbo Zhang, Jinshan Li, Shumiao Zhao, Yingjun Li, Yunxiang Liang, Yongmei Hu

Journal: Microb Cell Fact

PubMed ID: 35999638

Doi: 10.1186/s12934-022-01896-0

Products & Services: Gene Fragments

N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein products

Authors: Kyusik Q Kim, Bhagyashri D Burgute, Shin-Cheng Tzeng, Crystal Jing, Courtney Jungers, Junya Zhang, Liewei L Yan, Richard D Vierstra, Sergej Djuranovic, Bradley S Evans, Hani S Zaher

Journal: Cell Rep

PubMed ID: 35988540

Doi: 10.1016/j.celrep.2022.111300

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Myoglobin diet affected the colonic mucus layer and barrier by increasing the abundance of several beneficial gut bacteria

Authors: Shuai Jiang, Dejiang Xue, Miao Zhang, Qian Li, Hui Liu, Di Zhao, Guanghong Zhou, Chunbao Li

Journal: Food Funct

PubMed ID: 35943300

Doi: 10.1039/d2fo01799g

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Transient Complexity of Lipidome Is Explained by Fatty Acyl Synthesis and Cyclopropanation

Authors: Nikolay V Berezhnoy, Amaury Cazenave-Gassiot, Liang Gao, Juat Chin Foo, Shanshan Ji, Viduthalai Rasheedkhan Regina, Pui Khee Peggy Yap, Markus R Wenk, Staffan Kjelleberg, Thomas William Seviour, Jamie Hinks

Journal: Metabolites

PubMed ID: 36144187

Doi: 10.3390/metabo12090784

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The Biosynthesis of 1-octene-3-ol by a Multifunctional Fatty Acid Dioxygenase and Hydroperoxide Lyase in

Authors: Tongfu Su, Yuannan Chen, Haohao Liu, Yuqian Gao, Jiawen Guo, Yanan Li, Yuancheng Qi, Liyou Qiu

Journal: J Fungi (Basel)

PubMed ID: 36012815

Doi: 10.3390/jof8080827

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Hydrogel Injection Molding to Generate Complex Cell Encapsulation Geometries

Authors: Amy E Emerson, Alec B McCall, Sarah R Brady, Emily M Slaby, Jessica D Weaver

Journal: ACS Biomater Sci Eng

PubMed ID: 36044604

Doi: 10.1021/acsbiomaterials.2c00640

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Immunoprophylaxis using polypeptide chimera vaccines plus adjuvant system promote Th1 response controlling the spleen parasitism in hamster model of visceral leishmaniasis

Authors: Miriã Rodrigues Gusmão, Thaís Lopes Valentim Di Paschoali Ostolin, Lívia Mendes Carvalho, Ana Flávia Pereira Costa, Gabriel José Lucas Moreira, Jamille Mirelle de Oliveira Cardoso, Rodrigo Dian de Oliveira Aguiar-Soares, Alexandre Barbosa Reis, Rory Cristiane Fortes de Brito, Bruno Mendes Roatt

Journal: Vaccine

PubMed ID: 35963820

Doi: 10.1016/j.vaccine.2022.08.005

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Ubiquitination at the lysine 27 residue of the Parkin ubiquitin-like domain is suggestive of a new mechanism of Parkin activation

Authors: Jun-Yi Liu, Tsuyoshi Inoshita, Kahori Shiba-Fukushima, Shigeharu Yoshida, Kosuke Ogata, Yasushi Ishihama, Yuzuru Imai, Nobutaka Hattori

Journal: Hum Mol Genet

PubMed ID: 35313349

Doi: 10.1093/hmg/ddac064

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Neuroprotection of Bone Marrow-Derived Mesenchymal Stem Cell-Derived Extracellular Vesicle-Enclosed miR-410 Correlates with HDAC4 Knockdown in Hypoxic-Ischemic Brain Damage

Authors: Mingqi Shen, Rongxiu Zheng, Xuan Kan

Journal: Neurochem Res

PubMed ID: 36028735

Doi: 10.1007/s11064-022-03670-5

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Pathway elucidation of bioactive rhamnosylated ginsenosides in Panax ginseng and their de novo high-level production by engineered Saccharomyces cerevisiae

Authors: Chaojing Li, Xing Yan, Zhenzhen Xu, Yan Wang, Xiao Shen, Lei Zhang, Zhihua Zhou, Pingping Wang

Journal: Commun Biol

PubMed ID: 35918414

Doi: 10.1038/s42003-022-03740-y

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Identification of the extracellular metallo-endopeptidases ADAM and ADAMTS in the yellow fever mosquito Aedes aegypti

Authors: Christie S Herd, Xiudao Yu, Yingjun Cui, Alexander W E Franz

Journal: Insect Biochem Mol Biol

PubMed ID: 35932972

Doi: 10.1016/j.ibmb.2022.103815

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Development of a pentavalent broadly protective nucleoside-modified mRNA vaccine against influenza B viruses

Authors: Norbert Pardi, Juan Manuel Carreño, George O'Dell, Jessica Tan, Csaba Bajusz, Hiromi Muramatsu, Willemijn Rijnink, Shirin Strohmeier, Madhumathi Loganathan, Dominika Bielak, Molly M H Sung, Ying K Tam, Florian Krammer, Meagan McMahon

Journal: Nat Commun

PubMed ID: 35945226

Doi: 10.1038/s41467-022-32149-8

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Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus

Authors: Natael M Wayllace, Nicolas Hedín, María V Busi, Diego F Gomez-Casati

Journal: Biotechnol Lett

PubMed ID: 35997915

Doi: 10.1007/s10529-022-03296-1

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Microfluidic-Derived Detection of Protein-Facilitated Copper Flux Across Lipid Membranes

Authors: Kamil Górecki, Jesper S Hansen, Ping Li, Niloofar Nayeri, Karin Lindkvist-Petersson, Pontus Gourdon

Journal: Anal Chem

PubMed ID: 35969432

Doi: 10.1021/acs.analchem.2c02081

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Reduced sister chromatid cohesion acts as a tumor penetrance modifier

Authors: Jun Wang, Holly R Thomas, Yu Chen, Stefanie M Percival, Stephanie C Waldrep, Ryne C Ramaker, Robert G Thompson, Sara J Cooper, Zechen Chong, John M Parant

Journal: PLoS Genet

PubMed ID: 35994499

Doi: 10.1371/journal.pgen.1010341

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Novel Hits for N-Myristoyltransferase Inhibition Discovered by Docking-Based Screening

Authors: Danislav S Spassov, Mariyana Atanasova, Irini Doytchinova

Journal: Molecules

PubMed ID: 36080246

Doi: 10.3390/molecules27175478

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Water-organizing motif continuity is critical for potent ice nucleation protein activity

Authors: Jordan Forbes, Akalabya Bissoyi, Lukas Eickhoff, Naama Reicher, Thomas Hansen, Christopher G Bon, Virginia K Walker, Thomas Koop, Yinon Rudich, Ido Braslavsky, Peter L Davies

Journal: Nat Commun

PubMed ID: 36028506

Doi: 10.1038/s41467-022-32469-9

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Second-Generation SuptoxR Strains for High-Level Recombinant Membrane Protein Production

Authors: Eleni Vasilopoulou, Artemis Giannakopoulou, Charalampos Kapsalis, Myrsini Michou, Aristeidis Michoglou-Sergiou, Fragiskos N Kolisis, Georgios Skretas

Journal: ACS Synth Biol

PubMed ID: 35922033

Doi: 10.1021/acssynbio.1c00598

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