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And then, proteins were resolved in 4–12% Bis-Tris SurePAGE gel (Genscript) and transferred to 0.45 µm PVDF membrane (Millipore) for blotting.

Authors: Sitao Zhang, Zixuan Jiang, Rundong Huang, Wenting Huang, Siyuan Peng, Shaoxun Mo, Letao Zhu, Peiheng Li, Ziyi Zhang, Emily Pan, Xi Chen, Yunfei Long, Qi Liang, Jin Tang, Renjing Xu, Rui Qing

Journal: Advanced Science

Doi: 10.1002/advs.202502723

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High throughput cryo-EM provides structural understanding for modulators of the lysosomal ion channel TRPML1

Authors: Judith Reeks, Pravin Mahajan, Mellissa Clark, Suzanna R Cowan, Elena Di Daniel, Christopher P Earl, Samantha Fisher, Rhian S Holvey, Scott M Jackson, Emyr Lloyd-Evans, Carmine M Morgillo, Paul N Mortenson, Marc O'Reilly, Caroline J Richardson, Patrick Schöpf, Daniel M Tams, Helen Waller-Evans, Simon E Ward, Stuart Whibley, Pamela A Williams, Christopher N Johnson

Journal: Structure

PubMed ID: 40532704

Doi: 10.1016/j.str.2025.05.014

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Heterologous synthesis of a simplified nitrogenase analog in Escherichia coli

Authors: Yiling A Liu, Chi Chung Lee, Kamil Górecki, Martin T Stiebritz, Calder Duffin, Joseph B Solomon, Markus W Ribbe, Yilin Hu

Journal: SCIENCE ADVANCES

PubMed ID: 40315313

Doi: 10.1126/sciadv.adw6785

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Identification of naturally occurring drug-resistant mutations of SARS-CoV-2 papain-like protease

Authors: Haozhou Tan, Qianru Zhang, Kyriakos Georgiou, Siyu Zhang, Kan Li, George Lambrinidis, Antonios Kolocouris, Xufang Deng, Jun Wang

Journal: Nature Communications

PubMed ID: 40379662

Doi: 10.1038/s41467-025-59922-9

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A hidden cysteine in Fis1 targeted to prevent excessive mitochondrial fission and dysfunction under oxidative stress

Authors: Suman Pokhrel, Gwangbeom Heo, Irimpan Mathews, Shun Yokoi, Tsutomu Matsui, Ayori Mitsutake, Soichi Wakatsuki, Daria Mochly-Rosen

Journal: Nature Communications

PubMed ID: 40328741

Doi: 10.1038/s41467-025-59434-6

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Structural visualization of HECT-type E3 ligase Ufd4 accepting and transferring

Authors: Xiangwei Wu, Huasong Ai, Junxiong Mao, Hongyi Cai, Lu-Jun Liang, Zebin Tong, Zhiheng Deng, Qingyun Zheng, Lei Liu, Man Pan

Journal: nature COMMUNICATION

PubMed ID: 40341121

Doi: doi.org/10.1038/s41467-025-59569-6

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Fe(III)−H2O2 as an Alternative Oxidant for Catalytic Sulfoxidation in Cytochrome P450 Enzymes: Combined Theoretical and Experimental Evidence

Authors: Musen Li, Langxing Liao, Zhihui Jiang, Zongyu Bao, Shengbiao Ji, Binju Wang

Journal: ACS catalysis

Doi: doi.org/10.1021/acscatal.5c01673

Products & Services: Gene Synthesis Codon Optimization

Microtubules in Asgard archaea

Authors: Florian Wollweber, Jingwei Xu, Rafael I Ponce-Toledo, Florina Marxer, Thiago Rodrigues-Oliveira, Anja Pössnecker, Zhen-Hao Luo, Jessie James Limlingan Malit, Anastasiia Kokhanovska, Michal Wieczorek, Christa Schleper, Martin Pilhofer

Journal: Cell

PubMed ID: 40120574

Doi: 10.1016/j.cell.2025.02.027

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Authors: Jingjing Wang, Yong Ma, Zimu Li, Hang Yuan, Banghui Liu, Zexuan Li, Mengzhen Su, Gul Habib, Yutong Liu, Lutang Fu, Peiyi Wang, Mei Li, Jun He, Jing Chen, Peng Zhou, Zhengli Shi, Xinwen Chen, Xiaoli Xiong

Journal: SCIENCE ADVANCES

PubMed ID: 40085715

Doi: 10.1126/sciadv.adr8772

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Integrative in silico and biochemical analyses demonstrate direct Arl3-mediated ODA16 release from the intraflagellar transport machinery

Authors: Jiaolong Wang, Rune T Kidmose, Niels Boegholm, Nevin K Zacharia, Mads B Thomsen, Anni Christensen, Tara Malik, Karl Lechtreck, Esben Lorentzen

Journal: The Journal of biological chemistry

PubMed ID: 39880089

Doi: 10.1016/j.jbc.2025.108237

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