| ￥3600 | |
| Z05239-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized ENPP-1, His, Human at 0.5μg/ml (100μl/well) on the plate can bind AntiENPP1 Antibody, hFc Tag. Test result was comparable to standard batch. Measured by its ability to hydrolyze thymidine 5'monophosphate pnitrophenyl ester. The specific activity is >40000 pmol/min/μg. Test result meets the standard. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 96.5 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 110-115 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized ENPP-1, His, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of 22.1 ng/ml determined by ELISA. »
ENPP-1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | Ectonucleotide pyrophosphatase/phosphodiesterase (ENPP)-1 is a membrane-bound protein that catalyzes the hydrolysis of extracellular nucleoside triphosphates to monophosphate and extracellular inorganic pyrophosphate (ePPi). Mechanical stimulation regulates ENPP-1 expression. |
| Synonyms | E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
