StrepCaptureXP™ Resin Lite

金斯瑞 StrepCaptureXP 亲和介质 Lite 版（货号 L01114）是一款专为 Strep-tag®II 和 Twin-Strep-tag® 重组蛋白的纯化而设计的亲和层析介质。本品在常用的蛋白表达系统中表现出卓越的纯化性能，尤其适用于膜蛋白的纯化。

价格	￥520 在线订购包邮(港澳台除外)
产品编号	L01114-1
规格	1 ml Resin (Total 2 ml) 5 ml Resin (Total 10 ml) 10 ml Resin (Total 20 ml) 25 ml Resin (Total 50 ml) 大单询价
数量
参考货期


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产品概述

Product description

Strep-tag® II（WSHPQFEK）和 Twin-Strep-tag®（WSHPQFEKGGGSGGGSGGSAWSHPQFEK）是广泛应用于基础研究、诊断和生物制药领域中重组蛋白纯化的亲和标签。
金斯瑞（GenScript）的 StrepCaptureXP™ Resin Lite（货号：L01114）是一款新一代亲和层析介质，专为高效纯化 Strep-tag® II 和 Twin-Strep-tag® 融合蛋白而设计。该填料采用具有自主知识产权的 StrepCaptureXP 配体（一种基因优化的链霉亲和素变体）进行功能化修饰，能够从复杂的生物裂解液（包括哺乳动物、细菌和昆虫表达系统来源的裂解液）中高亲和力、高特异性地捕获目标蛋白。
该填料具有结合温和且可逆的洗脱条件，确保最大程度地回收具有生物活性的蛋白。适用于需要温和处理的应用场景，例如膜蛋白、跨膜蛋白的纯化。

Key Features

● 高特异性，背景结合低。
● 兼容中性 pH 值和温和洗脱缓冲液。
● 良好的去污剂兼容性，适用于膜蛋白纯化。
● 适用于针对重力流层析、FPLC（快速蛋白液相色谱）及自动化纯化系统。

Selection Guide

	StrepCaptureXP Magnetic Beads (Cat. No.: L01075)	StrepCaptureXP Resin FF (Cat. No.: L01091)	StrepCaptureXP Resin Lite (Cat. No.: L01114)
Recommended Applications	High-throughput screening & small-scale purification	Scalable purification & process development	Routine research purification & small-scale protein preparation
If You Need	✓ High-throughput screening ✓ Automated parallel purification ✓ AI-driven protein screening and parallel evaluation of multiple protein candidates ✓ Fast turnaround and high first-pass success rate	✓ Scale-up purification ✓ Compatible with ÄKTA™ systems ✓ High stability for repeated purification cycles ✓ Cost-effective for long-term manufacturing and process development	✓ Flexible for small-scale protein preparation ✓ Easy-to-use format for manual purification ✓ Lower entry cost for routine research applications ✓ Challenging proteins including membrane proteins
Recommended Workflow	Automated magnetic purification platforms and parallel purification workflows	ÄKTA™ chromatography systems and scalable purification processes	Manual purification or small-scale chromatography workflows
Typical Scale	Small volume purification (Recommended: <1 mL beads equivalent)	Medium- to large-scale purification	Small-scale laboratory purification
Automation Compatibility	Compatible with automated magnetic purification platforms (e.g., AmMag™ Quatro 1300 )	Compatible with ÄKTA™ chromatography systems	Compatible with commonly used chromatography systems, including ÄKTA™ systems under recommended operating conditions (refer to Product Manual)
Binding Capacity	20–40 mg/mL beads	10–30 mg/mL resin	8–15 mg/mL resin
Reusability	≥30 purification cycles	≥100 purification cycles	≥35 purification cycles
Purity of Eluted Protein	≥90% (SDS-PAGE)	≥90% (SDS-PAGE)	≥90% (SDS-PAGE)

产品属性

Formulation	50%沉降树脂悬浮于含0.1% ProClin300的PBS缓冲液（pH 7.4）中
Matrix	4%高交联琼脂糖
Average Particle Size	45-165 μm
Pressure	0.1 Mpa
Ligand	StrepCaptureXP
Binding Capacity	8-15 mg蛋白/mL沉降树脂。（结合载量因目标蛋白而异）
Elution Method	使用50 mM生物素进行竞争性洗脱
Reusability	按照推荐方法进行再生和储存，可重复使用≥35次纯化循环
Reagents Compatibility	与常用裂解试剂兼容。
Storage	本品自生产之日起，若在 2-8°C下储存，可稳定保存1年。请勿在0°C或低于0°C下储存，请勿冷冻。

图例

Four Strep-tagged proteins were purified using StrepCaptureXP™ Resin Lite (GenScript, L01114) and a commercial affinity resin from Competitor-ST under identical purification conditions. Elution fractions were analyzed by SDS-PAGE.

M: Protein marker

Protein 1: AtmGFP-Strep II-Flag
Lane 1: GenScript StrepCaptureXP™ Resin Lite eluate (99.19% purity)
Lane 2: Competitor-ST eluate (79.03% purity)

Protein 2: AtmGFP-Strep II
Lane 3: GenScript StrepCaptureXP™ Resin Lite eluate (99.99% purity)
Lane 4: Competitor-ST eluate (99.99% purity)

Protein 3: Twin Strep-Brolucizumab
Lane 5: GenScript StrepCaptureXP™ Resin Lite eluate (97.13% purity)
Lane 6: Competitor-ST eluate (95.55% purity)

Protein 4: Strep-BAP-Flag
Lane 7: GenScript StrepCaptureXP™ Resin Lite eluate (98.90% purity)
Lane 8: Competitor-ST eluate (97.79% purity) »

Binding capacity was compared between StrepCaptureXP™ Resin Lite (GenScript, L01114) and a commercial affinity resin from Competitor-ST using six Strep-tagged proteins expressed in E. coli and CHO cells under identical purification conditions. »

SDS-PAGE analysis of HEK293-expressed ASIC2a membrane protein purification using StrepCaptureXP™ Resin Lite and a commercial affinity resin from Competitor-ST.

M: Protein marker
Lane 1: HEK293 cell lysate supernatant
Lanes 2–4: StrepCaptureXP™ Resin Lite purification (2: Flow Through; 3: Elution, 2 CV; 4: Elution, additional 3 CV)
Lanes 5–7: Competitor resin purification (5: Flow Through; 6: Elution, 2 CV; 7: Elution, additional 3 CV)
The target membrane protein (ASIC2a) has a theoretical molecular weight of 60.1 kDa.

Sample Preparation:
1. Cells were lysed in 20 mM HEPES, 150 mM NaCl (pH 7.4) containing 1–1.5% (w/v) LMNG for 2 h at 2–8°C.
2. The lysate was clarified by ultracentrifugation at 45,000 rpm for 45 min at 4°C.
3. 100 μL of resin was incubated with 12 mL of clarified membrane protein lysate for 2 h at 2–8°C.
4. After binding, washing and elution were performed according to the recommended purification protocol. Elution fractions were analyzed by SDS-PAGE. »

Binding capacity of StrepCaptureXP™ Resin Lite (GenScript, L01114) was evaluated after repeated regeneration with 0.1 M NaOH. No significant loss in binding capacity was observed after 40 regeneration cycles. »

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Terms of Use

本产品仅授予用户一项免费、不可转让且有限的使用许可，用户仅可按照本产品随附产品文件（包括但不限于使用说明书、用户手册、分析证书（CoA）、产品说明书、标签以及 GenScript 提供或引用的其他书面或电子资料）所规定的方式使用本产品，并仅可用于其声明的用途。任何超出上述范围的使用均可能需要另行获得 GenScript 的授权许可。本有限且不可转让的许可自用户通过 GenScript 或其授权经销商购买本产品时生效。
本许可声明不应被解释为对本产品适销性、特定用途适用性或商业用途适用性的任何保证。
未经 GenScript 事先书面授权，任何人不得复制、分发、修改、逆向工程、克隆、反向克隆、解析产品序列、进行再许可，或利用本产品开发任何与 GenScript 产品或服务具有竞争性的产品或服务。

第三方商标声明
本说明书中提及的所有产品及公司名称均为其各自所有者的商标或注册商标，包括但不限于：Strep-tag和Twin-Strep-tag为IBA GmbH的商标；ÄKTA为Global Life Sciences Solutions USA LLC及其关联公司或以Cytiva作为经营别称的关联公司的商标；Triton为The Dow Chemical Company的商标；Tween为Croda International Plc的商标。这些商标均归其各自所有者所有，提及仅为标识相关试剂或技术之目的。GenScript与上述商标所有者之间不存在任何赞助、背书或从属关系。

只可用于科研目的，不得用于人类或动物临床实验、诊断或治疗等医疗目的。