StrepCaptureXP Resin FF

GenScript’s StrepCaptureXP Resin FF (Cat. No. L01091) is a next-generation affinity chromatography medium engineered for the efficient purification of Strep-tag® II and Twin-Strep-tag® fusion proteins.

价格	￥980 在线订购包邮(港澳台除外)
产品编号	L01091-1
规格	1 ml Resin (Total 2 ml) 5 ml Resin (Total 10 ml) 10 ml Resin (Total 20 ml) 25 ml Resin (Total 50 ml) 大单询价
数量
参考货期


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产品概述

Product description

The Strep-tag® II (WSHPQFEK) and Twin-Strep-tag® (WSHPQFEKGGGSGGGSGGSAWSHPQFEK) are widely adopted affinity tags for the purification of recombinant proteins in basic research, diagnostics, and biomanufacturing.
GenScript’s StrepCaptureXP Resin FF (Cat. No. L01091) is a next-generation affinity chromatography medium engineered for the efficient purification of Strep-tag® II and Twin-Strep-tag® fusion proteins. Functionalized with an innovatively engineered StrepCaptureXP ligand—a genetically optimized streptavidin variant—this resin enables high-affinity, high-specificity capture proteins from complex biological lysates, including those derived from mammalian, bacterial, and insect expression systems.
The resin's high dynamic binding capacity, combined with mild and reversible elution conditions, ensures maximal recovery of biologically active proteins. It is especially suitable for applications requiring high purity and gentle handling, such as in the purification of membrane proteins, fragile multi-subunit complexes, or therapeutic candidates.

Key Features

● High specificity and low background binding
● Compatible with neutral pH and mild elution buffers
● Robust performance across a variety of expression hosts
● Optimized for gravity flow, FPLC, and automated purification systems

Selection Guide

	StrepCaptureXP Magnetic Beads (Cat. No.: L01075)	StrepCaptureXP Resin FF (Cat. No.: L01091)	StrepCaptureXP Resin Lite (Cat. No.: L01114)
Recommended Applications	High-throughput screening & small-scale purification	Scalable purification & process development	Routine research purification & small-scale protein preparation
If You Need	✓ High-throughput screening ✓ Automated parallel purification ✓ AI-driven protein screening and parallel evaluation of multiple protein candidates ✓ Fast turnaround and high first-pass success rate	✓ Scale-up purification ✓ Compatible with ÄKTA™ systems ✓ High stability for repeated purification cycles ✓ Cost-effective for long-term manufacturing and process development	✓ Flexible for small-scale protein preparation ✓ Easy-to-use format for manual purification ✓ Lower entry cost for routine research applications ✓ Challenging proteins including membrane proteins
Recommended Workflow	Automated magnetic purification platforms and parallel purification workflows	ÄKTA™ chromatography systems and scalable purification processes	Manual purification or small-scale chromatography workflows
Typical Scale	Small volume purification (Recommended: <1 mL beads equivalent)	Medium- to large-scale purification	Small-scale laboratory purification
Automation Compatibility	Compatible with automated magnetic purification platforms (e.g., AmMag™ Quatro 1300 )	Compatible with ÄKTA™ chromatography systems	Compatible with commonly used chromatography systems, including ÄKTA™ systems under recommended operating conditions (refer to Product Manual)
Binding Capacity	20–40 mg/mL beads	10–30 mg/mL resin	8–15 mg/mL resin
Reusability	≥30 purification cycles	≥100 purification cycles	≥35 purification cycles
Purity of Eluted Protein	≥90% (SDS-PAGE)	≥90% (SDS-PAGE)	≥90% (SDS-PAGE)

产品属性

Formulation	50% settled resin suspended in PBS (pH 7.2) with 0.1% ProClin300
Matrix	4% highly cross-linked agarose
Average Particle Size	90 μm (45-165 μm)
Pressure	0.3 MPa
Ligand	StrepCaptureXP
Binding Capacity	10-30 mg protein/mL settled resin. (Please note that the binding capacity is protein dependent)
Elution Method	Competitive elution with 50 mM Biotin
Reuse Capability	≥100 purification cycles when regenerated and stored as recommended.
Reagents Compatibility	Compatible with commonly used lysis reagents.
Application	purification of Strep-tag® II and Twin-strep-tag® proteins
Storage	Stored at 2°C~8°C. Do not store at 0°C or below 0°C to avoid freezing the resin.

图例

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Terms of Use

1) Intellectual Property Rights
This product may be covered by patents filed by GenScript Corporation. Purchase grants a non-transferable right to use the product and its components for research purposes only. Any resale, transfer, or commercial use is prohibited without prior authorization. For commercial licensing, contact product@genscript.com.

2) Third-Party Trademark Acknowledgement
All product and company names mentioned in this manual are trademarks or registered trademarks of their respective owners, including but not limited to: Strep-tag® II and Twin-Strep-tag® (registered trademarks of IBA GmbH), ÄKTA (registered trademark of Global Life Sciences Solutions USA LLC, Cytiva), Triton® X-100 and Triton® X-114 (registered trademarks of The Dow Chemical Company), Tween® 20 (registered trademark of Croda International Plc) , are the property of their respective owners and are used for identification purposes only. GenScript is not sponsored by, affiliated with, or endorsed by the owners of these trademarks.

For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.