目录产品 » 蛋白分离纯化 » 蛋白分离磁珠 » StrepCaptureXP Magnetic Beads

StrepCaptureXP Magnetic Beads

GenScript StrepCaptureXP Magnetic Beads (Cat. No. L01075) is designed for the high-throughput, efficient and automated purification of Strep-tag® II and Twin-strep-tag® proteins from commonly used protein expression systems including bacteria, yeast and mammalian cells.
¥3800 在线订购包邮(港澳台除外)
L01075-1

咨询产品问题
Product Description Strep-tag® II(WSHPQFEK)和 Twin-Strep-tag®(WSHPQFEKGGGSGGGSGGSAWSHPQFEK)是广泛应用于基础研究、诊断和生物制药领域中重组蛋白纯化的亲和标签。
金斯瑞(GenScript)的 StrepCaptureXP Magnetic Beads(货号:L01075)是一款新一代亲和层析介质,专为高效纯化带有 Strep-tag® II 和 Twin-Strep-tag® 的重组蛋白而设计。该填料表面修饰的是具有自主知识产权的 StrepCaptureXP 配体(一种基因优化的链霉亲和素变体),可实现对多种表达系统(如大肠杆菌、昆虫细胞、哺乳动物细胞)来源蛋白的高特异性捕获,背景低、回收率高,兼容自动化高通量流程。
Key Features ● 高特异性结合,低非特异吸附;
● 高耐碱性,可支持0.1 M – 0.5 M NaOH再生;
● 支持高通量与自动化纯化流程;
● 温和可逆的洗脱条件(50 mM Biotin),保持蛋白活性;
● 广泛缓冲液兼容性(pH 4 – 10,含还原剂、EDTA、去垢剂等30种特殊试剂);
● 适用于多种表达系统样本的纯化,例如细菌、昆虫、哺乳动物细胞等;
● 操作简便,避免了镍磁珠所需的多步洗杂。
Selection Guide
StrepCaptureXP Magnetic Beads (Cat. No.: L01075) StrepCaptureXP Resin FF (Cat. No.: L01091) StrepCaptureXP Resin Lite (Cat. No.: L01114)
Recommended Applications High-throughput screening & small-scale purification Scalable purification & process development Routine research purification & small-scale protein preparation
If You Need ✓ High-throughput screening
✓ Automated parallel purification
✓ AI-driven protein screening and parallel evaluation of multiple protein candidates
✓ Fast turnaround and high first-pass success rate
✓ Scale-up purification
✓ Compatible with ÄKTA™ systems
✓ High stability for repeated purification cycles
✓ Cost-effective for long-term manufacturing and process development
✓ Flexible for small-scale protein preparation
✓ Easy-to-use format for manual purification
✓ Lower entry cost for routine research applications
Challenging proteins including membrane proteins
Recommended Workflow Automated magnetic purification platforms and parallel purification workflows ÄKTA™ chromatography systems and scalable purification processes Manual purification or small-scale chromatography workflows
Typical Scale Small volume purification
(Recommended: <1 mL beads equivalent)
Medium- to large-scale purification Small-scale laboratory purification
Automation Compatibility Compatible with automated magnetic purification platforms (e.g., AmMag™ Quatro 1300 ) Compatible with ÄKTA™ chromatography systems Compatible with commonly used chromatography systems, including ÄKTA™ systems under recommended operating conditions (refer to Product Manual)
Binding Capacity 20–40 mg/mL beads 10–30 mg/mL resin 8–15 mg/mL resin
Reusability ≥30 purification cycles ≥100 purification cycles ≥35 purification cycles
Purity of Eluted Protein ≥90% (SDS-PAGE) ≥90% (SDS-PAGE) ≥90% (SDS-PAGE)
返回

Product Content 50%沉降磁珠悬浮于含0.1% ProClin300的PBS缓冲液(pH 7.2)中
Binding Capacity 20-40 mg蛋白/mL沉降磁珠。(实际结合载量因目标蛋白、标签类型、标签位置及分子大小而异)
Base Matrix 磁性琼脂糖微球(Magnetic agarose beads)
Particle Size 10-37 μm
Affinity Ligand StrepCaptureXP
Elution Method 竞争性洗脱(推荐使用50 mM Biotin洗脱缓冲液)
Reagent Compatibility 兼容常见裂解试剂
Reuse Capability ≥30次纯化循环(需按推荐方法进行再生和储存)
Storage 2°C - 8°C保存,自生产之日起有效期1年。严禁冷冻,请勿保存在0°C或低于0°C。
返回

StrepCaptureXP Magnetic Beads

»

StrepCaptureXP Magnetic Beads

»

<
>

Terms of Use 所有提及的产品和公司名称,例如 Strep-tag® 或 Twin-strep-tag®(IBA Lifesciences GmbH 的注册商标),均为其各自所有者的财产,GenScript 未获得 IBA 的赞助或认可。这些名称仅用于识别目的。
返回

只可用于科研目的,不得用于人类或动物临床实验、诊断或治疗等医疗目的。