GenCRISPR™ Cas9 Enzymes
DNA-free gene editing can be achieved by using purified Cas9 enzymes with gRNA and transfecting them directly into your cells or protoplasts of interest. GenCRISPR™ Cas9 nuclease is the recombinant Streptococcus pyogenes Cas9 protein purified from E. coli and can be used for genome editing by inducing site-specific double stranded breaks in double stranded DNA. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). Presence of a nuclear localization signal (NLS) fusion with Cas9 enzyme ensures nuclear localization.
Enzyme activity validation guarantee
The GenCRISPR™ Gene editing technology has been licensed-in from the Broad Institute. The activity of all GenCRISPR™ Cas9 nucleases has been validated by in-vitro or in-vivo assays. The bioactivity data for all enzymes is included in the protocol document.
|Nuclear localization and in vivo gene-editing||Double Strand Break||
|Single Strand Break||
|In vitro target DNA cleavage||GenCRISPR™ Cas9 nuclease|
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