| ￥6400 | |
| Z03622 | |
|
|
|
|
|
|
|
|
|
The GenCRISPR™ Ultra eSpCas9 product line provides customers with a selection of research use, basic GMP and GMP compliant Cas9 nucleases. The Cas9 protein can be formed with the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex to perform gene editing has been shown to reduce the challenges encountered with other CRISPR gene editing techniques such as viral and plasmid delivery. Challenges include off-target effects, cell viability and transcription/translational challenges. GenCRISPR™ Ultra eSpCas9-2NLS is a mutant form of Cas9 nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with double-ended nuclear localization signal (NLS). GenCRISPR™ Ultra eSpCas9-2NLS delivers higher fidelity and less off-target activity than wild-type SpCas9 nuclease. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
| Key Features |
High knockout
efficiencies: Consistent high performance in in-vitro plasmid cleavage test. Tag-free: No extra tag amino acid Animal-free: Plant-based culture media, Enzyme-free chromatographic purification. DNA-free: No external DNA added to the system. |
|
gRNA-dependent double-stranded DNA cleavage |
| Expression System |
Recombinant
Cas9 with double-ended NLS expressed by E.coli |
| Species |
S. pyogenes |
| Tag |
Tag-free |
| Molecular Weight |
~160
kDa |
| Concentration |
10
mg/mL |
| Active temperature |
This
eSpCas9 is active at 37°C. |
| Formulation |
Supplied
as a solution of 25 mM Tris, 300 mM NaCl,
0.1 mM EDTA, 50% Glycerol pH 8.0 at 25°C. |
| Storage & Stability | This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles. |
| Appearance |
Clear, colorless liquid |
| Purity |
≥ 95% as analyzed by SDS-PAGE ≥ 90% as analyzed by SEC-HPLC |
| Concentration |
10 mg/ml±1 mg/ml |
| Bioactivity |
≥ 90% |
| Residual DNase |
Non-specific DNase activity |
| Residual RNase |
Non-specific RNase activity |
| Endotoxin Level |
≤ 10 EU/mg as analyzed by gel clotting method |
A 20 μl reaction in 1×Cas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng GenCRISPR™ Ultra eSpCas9-2NLS for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis. »
