| RC00001 | |
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CRISPR (clustered regularly interspaced short palindromic repeat) is an
adaptive immune system that provides protection against mobile genetic
elements (viruses, transposable elements and conjugative plasmids).
CRISPR clusters contain spacers, sequences complementary to antecedent
mobile elements, and target invading nucleic acids. CRISPR clusters are
transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR
systems correct processing of pre-crRNA requires a trans-encoded small
RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
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Genome editing with CRISPR T-Cells, Stem Cells, etc. |
| Appearance | Clear liquid |
| Purity |
≥ 95.0% by SEC-HPLC, ≥ 95.0% by Bis-Tris PAGE |
| Quality |
Antibiotic-free and animal-free production |
| Concentration |
9.5-12.5 mg/mL |
| Bioactivity |
cleavage activity>85% |
| Residual RNase |
Negative |
| Endotoxin Level |
≤ 10.0EU/mg |
| Synonyms |
CRISPR-associated endonuclease Cas9/Csn1, cas9, SpCas9, SpyCas9 |
| Source |
This product has undergone codon optimization, nuclear localization signal (NLS) design, and optimization of E. coli expression and purification. |
| Species |
Streptococcus pyogenes |
| Tag | His tag |
| Molecular Weight |
163
kDa |
| Concentration |
10 mg/ml |
| Active temperature |
This Cas9 is active at 37°C. |
| Formulation |
Supplied as a solution of 30mM Tris-HCl, 0.3M NaCl, 50% Glycerol, 0.1 mM EDTA, pH 7.4 |
| Storage & Stability |
This product remains stable for up to 18 months at -20±5°C. Avoid repeated freeze-thaw cycles. |
