| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | ) with PEG-dithiol molecules (3400 MW, Laysan Biosciences, SH-PEG-SH-3400) 28 or a matrix metalloproteinase (MMP)-degradable peptide (KCGGPQGIAGQGCK; Genscript, >90% purity, C-terminus amidated) 57 . To promote cell adhesion, 3 4 mM CRGDS 52 (Genscript, >90% purity, C-terminus amidated) was incorporated through metalloproteinase (MMP)-degradable peptide (KCGGPQGIAGQGCK; Genscript, >90% purity, C-terminus amidated) 57 . To promote cell adhesion, 3 4 mM CRGDS 52 (Genscript, >90% purity, C-terminus amidated) was incorporated through the thiol of a terminal cysteine group. Frozen stock solutions of 8-arm PEG-NB were prepared | Get A Quote |
Botulinum neurotoxin (BoNT) detection provides a useful model for validating cell-based neurotoxicity screening approaches, as sensitivity is dependent on functionally competent neurons and clear quantitative endpoints are available for correlating results to approved animal testing protocols. Here, human induced pluripotent stem cell (iPSC)-derived neuronal cells were cultured on chemically-defined poly(ethylene glycol) (PEG) hydrogels formed by thiol-ene photopolymerization and tested as a cell-based neurotoxicity assay by determining sensitivity to active BoNT/A1. BoNT/A1 sensitivity was comparable to the approved in vivo mouse bioassay for human iPSC-derived neurons and neural stem cells (iPSC-NSCs) culture... More
