| Products/Services Used | Details | Operation |
|---|---|---|
| Protein and Antibody Isolation | cells were harvested and lysed in buffer A (20 mM Tris, 100 mM NaCl, 10 mM imidazole, pH 8.0). The supernatant was loaded onto a nickel-chelating column (GenScript) washed with buffer A, followed by elution with buffer B (20 mM Tris, 100 mM NaCl, 250 mM imidazole, pH 8.0). The eluted protein solution was desalted | Get A Quote |
As the COVID-19 pathogen, SARS-CoV-2 relies on its main protease (M Pro ) for pathogenesis and replication. During the crystallographic analyses of M Pro crystals that were exposed to the air, a uniquely Y-shaped, S-O-N-O-S-bridged posttranslational crosslink that connects three residues C22, C44, and K61 at their side chains was frequently observed. As a novel covalent modification, this crosslink serves potentially as a redox switch to regulate the catalytic activity of M Pro , a demonstrated drug target of COVID-19. The formation of this linkage leads to a much more opened active site that can be potentially targeted for the development of novel SARS-CoV-2 antivirals. The structural rearrangement of M Pro by... More
