Powdery mildew, caused by f. sp. (), is one of the most important diseases on wheat worldwide and can lead to a large reduction in wheat production. Class III peroxidases (PODs), a kind of secretory enzyme and members of a multigene family in higher plants, have been linked to various plant physiological functions and defensive responses. However, the role of PODs in wheat resistance to remains unclear. , a class III POD gene, was identified from the proteomics sequencing of the incompatible interaction between wheat () cultivar Xingmin 318 and isolate E09. After transient expression of the TaPOD70-GFP fusion protein in leaves, TaPOD70 was located in the membrane region. Yeast secretion assay showed that T... More
Powdery mildew, caused by f. sp. (), is one of the most important diseases on wheat worldwide and can lead to a large reduction in wheat production. Class III peroxidases (PODs), a kind of secretory enzyme and members of a multigene family in higher plants, have been linked to various plant physiological functions and defensive responses. However, the role of PODs in wheat resistance to remains unclear. , a class III POD gene, was identified from the proteomics sequencing of the incompatible interaction between wheat () cultivar Xingmin 318 and isolate E09. After transient expression of the TaPOD70-GFP fusion protein in leaves, TaPOD70 was located in the membrane region. Yeast secretion assay showed that TaPOD70 was a secretory protein. Furthermore, Bax-induced programmed cell death was inhibited by transient expression of in . The transcript expression level of was significantly upregulated in the wheat- compatible interaction. More crucially, knocking down using virus-induced gene silencing increased wheat resistance to compared with the control plants. In response to , histological analyses indicated that hyphal development of was significantly reduced, whereas HO production was enhanced in -silenced leaves. These findings imply that TaPOD70 may act as a susceptibility factor, adversely regulating wheat resistance to .
