至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Structural characterization and immunogenicity evaluation of amphibian-derived collagen type II from the cartilage of Chinese Giant Salamander ()

J Biomater Sci Polym Ed. 2020; 
Jianlin Luo, Xiaojing Yang, Yu Cao, Guoyong Li, Yonglu Meng, Can Li
Products/Services Used Details Operation
Molecular Biology Reagents … (Shanghai, China). Protein marker (5-245 kDa) and the standard CT-II from bovine nasal septum were purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). Precast gels of 4-12% were supported by Genscript Co., Ltd. (Nanjing, China) … Get A Quote

摘要

Collagen type II (CT-II) has unique biological activities and functions, yet the knowledge on amphibian-derived CT-II is rare. Herein, acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were successfully isolated and characterized from the cartilage of Chinese Giant Salamander (CGS). The immunogenicity of collagen was then evaluated and compared with that of the standard bovine CT-II (SCT-II) by T-lymphocyte cell proliferation activity. Results demonstrated that ASC and PSC were predominantly CT-II along with minor collagen type I and maintained intact triple-helical structure of nature collagen. Compared with SCT-II, higher glycine content (337.80 and 339.93 residues/1000 residues) and lower degree... More

关键词

biomedical material, Chinese Giant Salamander (Andrias davidianus), Collagen type II, cartilage, immunogenicity