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Novel association of DJ-1 with HER3 potentiates HER3 activation and signaling in cancer

Oncotarget. 2016-10-01; 
Shu Zhang, Seema Mukherjee, Xuejun Fan, Ahmad Salameh, Kalpana Mujoo, Zhao Huang, Leike Li, Georgina To'a Salazar, Ningyan Zhang, Zhiqiang An
Products/Services Used Details Operation
Custom Vector Construction … For stable DJ-1 overexpressing cell line construction, the pcDNA3/FRT vector (GenScript) containing the human DJ-1 was used to transfect cancer cells (MCF-7 and T47-D). Transfected cells were selected by the addition of G418 (20 μg/ml) to culture medium for 3-4 weeks … Get A Quote

摘要

HER3/ErbB3 has emerged as a new therapeutic target for cancer. Currently, more than a dozen anti-HER3 antibodies are in clinical trials for treatment of various cancers. However, limited understanding of the complex HER3 signaling in cancer and lack of established biomarkers have made it challenging to stratify cancer patients who can benefit from HER3 targeted therapies. In this study, we identified DJ-1/PARK7 (Parkinson Protein 7) as a novel interaction partner of HER3 and demonstrated the potential of DJ-1 as a biomarker for anti-HER3 cancer therapy. DJ-1 association with HER3 protects HER3 from ubiquitination and degradation through the proteasomal pathway in breast cancer cells. However, neuregulin 1 (NRG-... More

关键词

DJ-1, HER3, anti-HER3 monoclonal antibody, biomarker, cancer