| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … K 15 )]. The sequences were purchased as synthetic genes, which were cloned into pUC57 from GenScript (Piscataway, NJ). Ends were designed to be flanked by SpeI and NheI restriction sites, respectively. pUC57 derivatives … | Get A Quote |
Non-viral gene delivery systems are important transport vehicles that can be safe and effective alternatives to currently available viral systems. A new family of multifunctional spider silk-based gene carriers was bioengineered and found capable of targeting human mesenchymal stem cells (hMSCs). These carriers successfully delivered DNA to the nucleus of these mammalian cells. The presence of specific functional sequences in the recombinant proteins, such as a nuclear localization sequence (NLS) of the large tumor (T) antigen of the Simian virus 40 (SV40 ), an hMSC high affinity binding peptide (HAB), and a translocation motif (TLM) of the hepatitis-B virus surface protein (PreS2), and their roles in mitigatio... More
