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A Rat Basophilic Leukaemia cell sensor for the detection of pathogenic viruses.

Biosens Bioelectron.. 2013-05; 
Qu M, Boruah BM, Zhang W, Li Y, Liu W, Bi Y, Gao GF, Yang R, Liu D, Gao B. a CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, Chinab University of Chinese Academy of Sciences, Beijing, Chinac China-Japan Joint Laboratory of Molecular Virology and Immunology, Chinese Academy of Sciences, Beijing, Chinad State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China
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Gene Synthesis The gene for mouse IgE-Fc fragment containing C3e and C4e domains was synthesized by Genscript and fused to selected sdAb with a poly-glycine linker. Get A Quote

摘要

A Rat Basophilic Leukemia (RBL) cell sensor is developed for the detection and identification of pathogenic viruses. Recombinant sdAb-Fc antibodies were constructed by linking virus-specific single domain antibody to mouse IgE-Fc fragment. The sdAb-Fc can bind to FcepsilonRI receptors on RBL cells and can be cross-linked by target viruses leading to cell activation and Ca2+ influx reflected by the increase of intracellular fluorescence. The responses of RBL cells to viruses in real time could be observed using fluorescence microscopy. 103 TCID50 of H5N1 viruses and 10 LD50 of rabies viruses could be detected in less than three minutes. An excess quantity of non-relevant viruses did not interfere with the recogn... More

关键词

Cell sensor; RBL cell; IgE; Single domain antibody; H5N1; Rabies virus
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