Establishment of a scalable manufacturing platform for in-silico-derived ancestral adeno-associated virus vectors
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Cells were seeded 1 day (shake flask) or 2–3 days (3 l and 50 l SUB) before transfection, to target achieving a cell density from 1E+06 to 3E+06 vc/ml at transfection. The plasmids used for the transfection were pHelper (Takara Bio, Moun - tain View, CA), pAAV-GFP (Cell Biolabs, San Diego, CA) and pRep/ Cap for Anc80 (Vandenberghe’s lab, Boston, MA) or AAV8 (Synthe - sized by GenScript, Piscataway, NJ, USA). |
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