| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Amplified PCR products were analysed on a 1% agarose gel stained with 3 µl (0.5 µg ml-1) ethidium bromide in 1× TAE buffer at 70V for 1.5 hours. The gel was then visualised under a UV trans-illuminator. The amplicons of expected sizes of approximately 200 bp were scored on the gel and purified using Quick Clean II gel Purification kit (GenScript, UK), following manufacturer’s instructions. The purified PCR products were sequenced using respective IYSV forward and reverse primers. Sequencing of the PCR product was performed by the dideoxynucleotide chain termination method (Sangler et al., 1977) at Macrogen, Seoul, Korea. Consensus sequence was generated using Geneious Pro 5.5.6 Software (Bio matters Ltd., Auckland, NZ). | Get A Quote |
Thrips-transmitted Iris yellow spot virus (IYSV) (Family Tospoviridae, Genus Orthotospovirus) is a major constraint to onion (Allium cepa L.) production in Kenya. Determining seasonal patterns of the vector and alternate hosts of the virus could help onion farmers plan Integrated Pest Management strategies; while allowing them to move away from calendar-based applications of insecticides. The objective of this study was to determine the distribution, seasonal variations and alternate hosts of vector and IYSV. For distribution, a survey was carried out on a network of farms in all onion growing areas in Kenya; while for seasonality, surveys were done in two areas; Loitoktok and Naivasha. Data were collected on I... More
