| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Pentetrazol (PTZ) was purchased from Sigma-Aldrich Trade Co. Ltd. Air-dried Dendrobium officinale purchased from Baise Biological Products Chain Co. Ltd. in Lingyun County, Baise, was used to prepare 0.15 g/l diluent based on Yu et al.’s method of extraction, purification, and concentration determination of DOPS [10]. IL-1β, TNF-α, and β-actin primers were synthesized by GenScript Biotechnology Co. Ltd. The primer sequences are seen in Table 1. Kits for total RNA extract were purchased from Shanghai Doctor Biotechnology Co. Ltd. Fluorescence quantitative PCR kits were purchased from TaKaRa Company. Rat IL-1β and TNF-α ELISA kits were purchased from Invitrogen Company. Rat p-ERK1/2, p-JNK, and p-p38 ELISA kits were purchased from RayBiotech Company. Rat p38, ERK1/2, and MKP-1 ELISA kits were purchased from LifeSpan BioSciences. Rat JNK ELISA kits were purchased from R&D Systems. | Get A Quote |
Objective. To investigate the effects of Dendrobium officinale polysaccharides (DOPS) on the expression of inflammatory factors IL-1β and TNF-α and the MKP-1/MAPK signal pathway. Methods. PTZ-induced epileptic rat models were established. The rats were randomly divided into four groups: the control group, the DOPS group, the model group, and the DOPS intervention group. RT-PCR was used to measure the mRNA expression of IL-1β and TNF-α in the hippocampi of all groups; western blot was used to measure the protein expression of IL-1β and TNF-α and phosphorylation of ERK1/2, JNK, p38, and MKP-1 in the hippocampi of all groups at weeks 1, 2, 3, and 4 after modeling. Results. At weeks 1, 2, 3, and 4 aft... More
