| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | Materials and Methods Preparation of proteins The gene encoding D2-L29, optimized for expression in Escherichia coli, was synthesized by Genscript, and cloned into the restriction sites between NcoI and NotI of the expression vector pHT1, a modified vector from pRA2 (Makabe et al. | Get A Quote |
Computer-guided library generation is a plausible strategy to optimize antibodies. Herein, we report the improvement of the affinity of a single-domain camelid antibody for its antigen using such approach. We first conducted experimental and computational alanine scanning to describe the precise energetic profile of the antibody-antigen interaction surface. Based on this characterization, we hypothesized that in-silico mutagenesis could be employed to guide the development of a small library for phage display with the goal of improving the affinity of an antibody for its antigen. Optimized antibody mutants were identified after three rounds of selection, in which an alanine residue at the core of the antibody-a... More
