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A highly tunable system for the simultaneous expression of multiple enzymes in Saccharomyces cerevisiae

ACS Synth Biol. 2015; 
Ito Y, Yamanishi M, Ikeuchi A, Matsuyama T.
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摘要

Control of the expression levels of multiple enzymes in transgenic yeasts is essential for the effective production of complex molecules through fermentation. Here, we propose a tunable strategy for the control of expression levels based on the design of terminator regions and other gene-expression control elements in Saccharomyces cerevisiae. Our genome-integrated system, which is capable of producing high expression levels over a wide dynamic range, will broadly enable metabolic engineering and synthetic biology. We demonstrated that the activities of multiple cellulases and the production of ethanol were doubled in a transgenic yeast constructed with our system compared with those achieved with a standard ex... More

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