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Import of a major mitochondrial enzyme depends on synergy between two distinct helices of its presequence.

Biochem J. 2016; 
Kalef-Ezra E, Kotzamani D, Zaganas I, Katrakili N, Plaitakis A, Tokatlidis K.
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Proteins, Expression, Isolation and Analysis Visualization of protein bands following western blotting was performed with the SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific, Rockford) and the LumiSensor (GenScript). Get A Quote

摘要

Mammalian glutamate dehydrogenase (GDH), a nuclear-encoded enzyme central to cellular metabolism, is among the most abundant mitochondrial proteins (constituting up to 10% of matrix proteins). To attain such high levels, GDH depends on very efficient mitochondrial targeting that, for human isoenzymes hGDH1 and hGDH2, is mediated by an unusually long cleavable presequence (N53). Here, we studied the mitochondrial transport of these proteins using isolated yeast mitochondria and human cell lines. We found that both hGDHs were very rapidly imported and processed in isolated mitochondria, with their presequences (N53) alone being capable of directing non-mitochondrial proteins into mitochondria. These presequences ... More

关键词

glutamate dehydrogenase; human; mitochondria; presequence; protein import