至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Evaluating the role of retinal membrane guanylyl cyclase 1 (RetGC1) domains in binding guanylyl cyclase-activating proteins (GCAPs).

J Biol Chem. 2015; 
Peshenko IV, Olshevskaya EV, Dizhoor AM.
Products/Services Used Details Operation
Custom Vector Construction … the 5′-CCGCGGTACTGCCTGTTTGGAGACACGGTCAACACCGCCTCGCGCATGGAGTCC ACCGGGCTGGGAGGAGGAGGAGGAGGA sequence and followed by the 5′-TGACGGCCG sequence were chemically synthesized and inserted into the pUC-KAN vector (Genscript) … Get A Quote

摘要

Retinal membrane guanylyl cyclase 1 (RetGC1) regulated by guanylyl cyclase-activating proteins (GCAPs) controls photoreceptor recovery and when mutated causes blinding disorders. We evaluated the principal models of how GCAP1 and GCAP2 bind RetGC1: through a shared docking interface versus independent binding sites formed by distant portions of the cyclase intracellular domain. At near-saturating concentrations, GCAP1 and GCAP2 activated RetGC1 from HEK293 cells and RetGC2(-/-)GCAPs1,2(-/-) mouse retinas in a non-additive fashion. The M26R GCAP1, which binds but does not activate RetGC1, suppressed activation of recombinant and native RetGC1 by competing with both GCAP1 and GCAP2. Untagged GCAP1 displaced both ... More

关键词

Eye; Guanylate Cyclase (Guanylyl Cyclase); Neurobiology; Photoreceptor; Signal Transduction