| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | Induction of Tfh Cells In Vitro Naive OVA-specific CD4+ T cells purified from OT-II mice were co-cultured with sorted CD8a+ or CD8a DCs (1:10 ratio of DC to T cells) for 1–6 days in the presence of 25 mg/ml poly(I:C), 100 ng/ml LPS, Flagellin, or R848 (Invivo- gen) ± 2 mM of OVA peptide (aa 323–339) (Genscript). | Get A Quote |
Recent studies on T follicular helper (Tfh) cells have significantly advanced our understanding of T cell-dependent B cell responses. However， little is known about the early stage of Tfh cell commitment by dendritic cells (DCs)， particularly by the conventional CD8α(+) and CD8α(-) DC subsets. We show that CD8α(-) DCs localized at the interfollicular zone play a pivotal role in the induction of antigen-specific Tfh cells by upregulating the expression of Icosl and Ox40l through the non-canonical NF-κB signaling pathway. Tfh cells induced by CD8α(-) DCs function as true B cell helpers， resulting in significantly increased humoral immune responses against various human pathogenic antigens， including ... More
