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Serine 25 phosphorylation inhibits RIPK1 kinase-dependent cell death in models of infection and inflammation.

Nat Commun. 2019-04; 
DondelingerYves,DelangheTom,PriemDario,Wynosky-DolfiMeghan A,SorobeteaDaniel,Rojas-RiveraDiego,GiansantiPiero,RoelandtRia,GropengiesserJulia,RuckdeschelKlaus,SavvidesSavvas N,HeckAlbert J R,VandenabeelePeter,BrodskyIgor E,BertrandMathieu
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Molecular Biology Reagents The sequences encoding WT RIPK1 and the mutated versions of RIPK1 were cloned into pENTR3C using the cloneEZ PCR cloning kit (GenScript). Get A Quote

摘要

RIPK1 regulates cell death and inflammation through kinase-dependent and -independent mechanisms. As a scaffold, RIPK1 inhibits caspase-8-dependent apoptosis and RIPK3/MLKL-dependent necroptosis. As a kinase, RIPK1 paradoxically induces these cell death modalities. The molecular switch between RIPK1 pro-survival and pro-death functions remains poorly understood. We identify phosphorylation of RIPK1 on Ser25 by IKKs as a key mechanism directly inhibiting RIPK1 kinase activity and preventing TNF-mediated RIPK1-dependent cell death. Mimicking Ser25 phosphorylation (S?>?D mutation) protects cells and mice from the cytotoxic effect of TNF in conditions of IKK inhibition. In line with their roles in IKK activatio... More

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