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Antigen delivery format variation and formulation stability through use of a hybrid vector.

Vaccine X. 2019-04; 
BeitelsheesMarie,HillAndrew,LiYi,ChenMingfu,AhmadiMahmoud Kamal,SmithRandall J,AndreadisStelios T,RostamiPooya,JonesCharles H,PfeiferBlai
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Gene Synthesis … corresponding target fragments were purified, ligated with T4 DNA ligase (Fermentas, Canada), and named as pMDA5, pCARD, pRD, pCARD-RD, pΔCARD and pΔRD, respectively (Table 2). Each of them was sent to a commercial company (Genscript, China) for sequencing … Get A Quote

摘要

A hybrid biological-biomaterial antigen delivery vector comprised of a polymeric shell encapsulating an core was previously developed for antigen production and subsequent delivery. Due to the engineering capacity of the bacterial core, the hybrid vector provides unique opportunities for immunogenicity optimization through varying cellular localization (cytoplasm, periplasm, cellular surface) and type (protein or DNA) of antigen. In this work, three protein-based hybrid vector formats were compared in which the pneumococcal surface protein A (PspA) was localized to the cytoplasm, surface, and periplasmic space of the bacterial core for vaccination against pneumococcal disease. Furthermore, we te... More

关键词

APCs, antigen presenting cells,AS, aqueous storage,CDM, chemically defined bacterial growth medium,CFA, Complete Freund's Adjuvant,CHV, cytoplasmic hybrid vector,CPSs, capsular polysaccharides,ClyA, cytolysin A,DNA vaccine,DS, desiccated storage,EHV, empty hybrid vector,IN, intranasal,IP, intraperitoneal,LBVs, live bacterial vectors,LLO, listeriolysin O,NVT, non-vaccine type,PAMPs, pathogen-associated molecular patterns,PCVs, pneumococcal conjugate vaccines,PHV, periplasmic hybrid vector,PcpA, pneumococcal choline-binding protein A,PhtD, histidine triad protein D,Pneumococcal disease,Pneumococcal surface protein A (PspA),PspA, pneumococcal surface protein A,SC, subcutaneous,SHV, surface hybrid vector,Streptococcus pneumoniae,Vaccine delivery,pHV, plasmid hybrid ve