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Heterologous expression， chaperone mediated solubilization and purification of parasitic nematode-specific growth factor-like protein of Setaria digitata.

Asian Pac J Trop Med. 2014;

RodrigoW Wp，DassanayakeR S，KarunanayakeE H，GunawardeneY I N Silva，WeerasenaO V

Products/Services Used	Details	Operation
Gene Synthesis	… vector. PCR amplification was performed using 10 ng of plasmid DNA in a 25 毺L reaction containing 0.5 unit of Taq DNA polymerase (GenScript, USA), 160 毺M of each dNTP (Promega) and 80 pM of each primers. Reaction …	Get A Quote

摘要

To clone， express and purify a putative parasitic nematode specific protein of Setaria digitata (S. digitata)， filarial nematode that infects livestock and cause significant economic losses in Far East and Asia to be used for structural and functional analyses.