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Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the Genus Tospovirus.

PLoS ONE. 2012; 
de OliveiraAthos Silva,MeloFernando Lucas,Inoue-NagataAlice Kazuko,NagataTatsuya,KitajimaElliot Watanabe,ResendeRenato Oliv
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Molecular Biology Reagents … agriculture in combination with the high prevalence of this virus in major soybean production areas in the United States has rendered … The PCR was performed in a Mastercycler ? (Eppendorf) using Taq polymerase (Genscript) at a concentration of 0.25 μl reaction consisting of 2 … Get A Quote

摘要

Tospoviruses (Genus Tospovirus, Family Bunyaviridae) are phytopathogens responsible for significant worldwide crop losses. They have a tripartite negative and ambisense RNA genome segments, termed S (Small), M (Medium) and L (Large) RNA. The vector-transmission is mediated by thrips in a circulative-propagative manner. For new tospovirus species acceptance, several analyses are needed, e.g., the determination of the viral protein sequences for enlightenment of their evolutionary history.

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