| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | Generation of PMIM-NS-Full and PMIM-NS-mut. A 931-bp cDNA containing the wt IAV PR8 (Mount Sinai) NS gene (898 bp) or its cDNA variant with mutated splicing elements (the 59, 39 splicing sites and the branch point) with 16-bp upstream and 17-bp downstream nucleotide sequences containing restriction enzyme sites was designed (sequence not shown), synthesized, and cloned into pCU57 vector (GenScript, Nanjing, China). | Get A Quote |
The importance of antiviral CD8 T cell recognition of alternative reading frame (ARF)-derived peptides is uncertain. In this study， we describe an epitope (NS1-ARF2) present in a predicted 14-residue peptide encoded by the +1 register of NS1 mRNA in the influenza A virus (IAV). NS1-ARF2 elicits a robust， highly functional CD8 T cell response in IAV-infected BALB/c mice. NS1-ARF2 is presented from unspliced NS mRNA， likely from downstream initiation on a Met residue that comprises the P1 position of NS1-ARF2 Derived from a 14-residue peptide with no apparent biological function and negligible impacts on IAV infection， infectivity， and pathogenicity， NS1-ARF2 provides a clear demonstration of how immu... More
