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Rapamycin Inhibits Oxidized Low Density Lipoprotein Uptake in Human Umbilical Vein Endothelial Cells via mTOR/NF-κB/LOX-1 Pathway.

PLoS ONE. 2016; 
ZhouYan-De，CaoXue-Qin，LiuZhi-Hua，CaoYong-Jun，LiuChun-Feng，ZhangYan-Lin，Xie
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Gene Synthesis Total RNA was extracted with TRIzol (Invitrogen, Carlsbad, CA, USA). Each sample was reversely transcribed into complementary DNA (cDNA) using a cDNA Synthesis kit (Fermentas, Vilnius, Lithuania) according to the manufacturer’s instructions. Q-PCR were performed on the ABI 7500 system (Applied Biosystems, Foster City, CA, USA) using an SYBR® green PCR Master Mix (Invitrogen, Carlsbad, CA, USA), and the following primers (GenScript, Nanjing, China): human LOX-1 (forward, 5′-TTACTCTCCATGGTGGTGCC-3′; reverse, 5′-AGCTTCTTCTGCTTGTTGCC-3′) and 18S (forward: 5′-TCAACACGGGAAACCTCAC-3′; reverse: 5′-CGCTCCACCAACTAAGAAC-3′). Get A Quote

摘要

Lectin-like oxidized low-density lipoprotein-1 (LOX-1) is the major receptor for oxidized low density lipoprotein (ox-LDL) uptake in human umbilical vein endothelial cells (HUVECs). Previously， we found that rapamycin inhibited ox-LDL accumulation in HUVECs， and this effect was related to its role in increasing the activity of autophagy-lysosome pathway. In this study， we determined whether rapamycin could also reduce ox-LDL uptake in HUVECs and investigated the underlying signaling mechanisms.

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