至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Antibody-antigen kinetics constrain intracellular humoral immunity.

Sci Rep. 2016; 
BottermannMaria,LodeHeidrun Elisabeth,WatkinsonRuth E,FossStian,SandlieInger,AndersenJan Terje,JamesL
Products/Services Used Details Operation
Custom Vector Construction … Vectors encoding h9C12 IgG1 variants were constructed by synthesizing gene fragments containing the desired mutations (Genscript, USA) and sub-cloned into either h9C12 pLNOH2-hIgG1-WT-OriP or h9C12 pLNOκ-hLC using the restriction sites BsmI and BsiWI … Get A Quote

摘要

During infection with non-enveloped viruses, antibodies stimulate immunity from inside cells by activating the cytosolic Fc receptor TRIM21. This intracellular humoral response relies on opsonized viral particles reaching the cytosol intact but the antigenic and kinetic constraints involved are unknown. We have solved the structure of a potent TRIM21-dependent neutralizing antibody in complex with human adenovirus 5 hexon and show how these properties influence immune activity. Structure-guided mutagenesis was used to generate antibodies with 20,000-fold variation in affinity, on-rates that differ by ~50-fold and off-rates by >175-fold. Characterization of these variants during infection revealed that TRI... More

关键词