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Surface-enhanced Raman scattering activity of negatively charged bio-analytes from a modified silver colloid

2018 Impact Factor 1.081 Spectroscopy Letters. 2016-02; 
C. Garrido, Boris E. Weiss-López & M. M. Campos Vallette
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Peptide Synthesis … Here, we study the SERS spectrum obtained with a new colloid. Experimental Materials Synthetic water-soluble peptides CGR, CGK, GEGEG, and GEKEG (98.8% purity) were purchased from (GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854 USA … Get A Quote

摘要

A modification to the traditional synthesis of reduced silver metal nanoparticles with hydroxylamine hydrochloride is proposed. The new colloid, obtained by decreasing the concentration of hydroxide and chloride ions, is stable in a large pH range. The low negative surface charge density of the nanoparticles allowed to obtain surface-enhanced Raman scattering (SERS) spectral activity of bio-analytes containing negatively charged groups in aqueous solution. The new colloidal system, characterized by electronic spectroscopy, atomic force microscopy, and zeta potential measurements, was used in the SERS study of the bio-analytes aspartic acid, four oligopeptides, one polypeptide and a probe, the organic dye fluore... More

关键词

Bio-analytes, negatively charged colloidal surface, silver nanoparticles, surface-enhanced Raman scattering