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Engineering HIV-resistant, anti-HIV chimeric antigen receptor T cells

Molecular Therapy. 2016; 
Malika Hale, Taylor Mesojednik, Guillermo S. Romano Ibarra, Jaya Sahni, Alison Bernard, Karen Sommer, Andrew M. Scharenberg,,, David J. Rawlings,,, and Thor A. Wagner,
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Gene Synthesis HIV scFvs were exchanged with that of the CD19 scFv in this plasmid as follows. scFvs from the previously reported human anti-HIV bNAbs PGT145, PGT128, 10E8, and VRC07-52351–53 were gene-synthesized by GenScript (Figure S3).Expression of viral transgenes (BFP+ ) and surface expression of the CD19 and VRC07-523-CARs, detected by staining cells with biotinylated Protein L (GenScript) and phycoerythrin (PE)-streptavidin (BD Biosciences), was confirmed by flow cytometry on an LSR II (BD Biosciences) 5–10 days after LV transduction or gene editing. Get A Quote

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