资源 » 参考数据库 » 客户发表文献

至今，GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次，处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果，再次证明GenScript 有能力帮助业内科学家Make research easy.

Discovery and characterisation of the automethylation properties of PRDM9

Biochemical Journa. 2017;

Xiaoying Koh-Stenta , Anders Poulsen , Rong Li , John Liang Kuan Wee , Perlyn Zekui Kwek , Sin Yin Chew , Jianhe Peng , Liling Wu , Ernesto Guccione , Joma Joy and Jeffrey Hill

Products/Services Used	Details	Operation
Custom Vector Construction	A panel of lysine-to-alanine PRDM9 singlemutant plasmids (K207A, K226A, K297A, K338A, K368A, K372A, K374A and K375A) were generated through synthesis of DNA-coding sequences followed by insertion into the pET21a(+) expression vector via the NdeI and BamHI insertion sites (GenScript).Inhibition assays of PRDM9 automethylation were performed by pre-incubating 5 mM PRDM9 with varying concentrations of suramin (Sigma–Aldrich), sinefungin (Sigma–Aldrich) or H3pNle (synthesised by GenScript) for 30 min at room temperature before the addition of SAM.	Get A Quote